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Effects of maternal nutrition on the expression of genomic imprinted genes inovine fetuses

Effects of maternal nutrition on the expression of genomic i...

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第十四届中国实验动物科学年会

作者： Jingyue (Ellie) Duan 张名媛 kaleigh flock Sahar Al Seesi Ion Mandoiu Amanda Jones Elizabeth Johnson Sambhu Pillai Maria Hoffman Katelyn McFadden Hesheng Jiang Sarah Reed Kristen Govoni Steve Zinn Zongliang Jiang Xiuchun (Cindy) Tian Department of Animal Science University of Connecticut College of Animal Science and Technology Guangxi University Laboratory Animal Center Guangxi Medical University Department of Computer Science University of Connecticut School of Animal Science Louisiana StateUniversity Agricultural Center

Genomic imprinting is an epigenetic phenomenon of differential allelic expression based on parentalorigin. To date, 263 imprinted genes have been identified among all investigated mammalian species.However, only 21 have been described in sheep, of which 11 are annotated in the current ovinegenome. Here, we aimtoi) use DNA/RNA high throughput sequencing to identify new monoallelicallyexpressed and imprinted genes in day 135 ovine fetuses and ii) determine whether maternal diet(100%, 60%, or 140% of National Research Council Total Digestible Nutrients) influences expression ofimprinted genes. We also reported strategies to solve technical challenges in the data analysispipeline. We identified 80 monoallelically expressed, 13 new putative imprinted genes, and fiveknown imprinted genes in sheep using the 263 genes stated above as a guide. Sanger sequencingconfirmed allelic expression of seven genes, CASD1, COPG2, DIRAS3, INPP5 F, PLAGL1, PPP1 R9 A, and SLC22 A18. Among the 13 putative imprinted genes, five were localized in the known sheep imprintingdomains of MEST on chromosome 4, DLK1/GTL2 on chromosome 18 and KCNQ1 on chromosome 21,and three were in a novel sheep imprinted cluster on chromosome 4, known in other species asPEG10/SGCE. The expression of DIRAS3, IGF2, PHLDA2, and SLC22 A18 was altered by maternal diet,albeit without allelic expression reversal. Together, our results expanded the list of sheep imprintedgenes to 34 and demonstrated that while the expression levels of four imprinted genes were changedby maternal diet, the allelic expression patterns were un-changed for all imprinted genes studied.

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