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Exploring the genetic control of sweat gland characteristics in beef cattle for enhanced heat tolerance

Journal of Animal Science and Biotechnology 2024年第5期15卷 1820-1831页

作者： Aakilah S.Hernandez Gabriel A.Zayas Eduardo E.Rodriguez kaitlyn m.sarlo davila Fahad Rafiq Andrea N.Nunez Cristiane Goncalves Titto Raluca G.mateescu Department of Animal Science North Carolina State UniversityRaleighNCUSA Department of Animal Sciences University of FloridaGainesvilleFLUSA. Ruminant Diseases and Immunology Research Unit National Animal Disease CentersUnited States Department of Agriculture1920 Dayton AvenueAmesIA 50010USA. Department of Animal Science Iowa StateAmesIAUSA School of Animal Sciences and Food Engineering University of Sao PauloAv Duque de Caxias Norte 225PirassunungaSao Paulo 13635‑900Brazil

Background Thermal stress in subtropical regions is a major limiting factor in beef cattle production systems with around$369 million being lost annually due to reduced *** stress causes numerous physiological and behavioral disturbances including reduced feed intake and decreased production *** utilize various physiological mechanisms such as sweating to regulate internal *** in these traits can help identify genetic variants that control sweat gland properties and subsequently allow for genetic selection of cattle with greater *** This study used 2,401 Brangus cattle from two commercial ranches in *** phenotypes that contribute to an animal's ability to manage heat stress were calculated from skin biopsies and included sweat gland area,sweat gland depth,and sweat gland *** animals were genotyped with the Bovine GGP F250K,and BLUPF90 software was used to estimate genetic parameters and for Genome Wide Association *** Sweat gland phenotypes heritability ranged from 0.17 to 0.42 indicating a moderate amount of the phenotypic variation is due to genetics,allowing producers the ability to select for favorable sweat gland properties.A weighted single-step GWAS using sliding 10 kb windows identified multiple quantitative trait loci(QTLs)explaining a significant amount of genetic *** located on BTA7 and BTA12 explained over 1.0%of genetic variance and overlap the ADGRV1 and CCDC168 genes,*** variants identified in this study are implicated in processes related to immune function and cellular proliferation which could be relevant to heat *** of Origin Alleles(BOA)were predicted using local ancestry in admixed populations(LAmP-LD),allowing for identification of markers'origin from either Brahman or Angus ancestry.A BOA GWAS was performed to identify regions inherited from particular ancestral breeds that might have a significant impact on sweat gland pheno

关键词： GWAS Heritability Skin Thermotolerance

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Ethyl methanesulfonate as Inductor of Somaclonal Variants in Different Crops

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Phyton-International Journal of Experimental Botany 2020年第4期89卷 835-850页

作者： Jose Gregorio Joya-davila F.A.Gutierrez-miceli Plant Biotechnology Laboratory Tecnologico Nacional de MexicoInstituto Tecnologico de Tuxtla GutierrezTuxtla Gutierrez29050Mexico

Ethyl methanesulfonate is a chemical mutagen,which is currently being used in plant breeding,to increase genetic variability in genes of agronomic interest,of species useful in *** primarily causes single base point mutations by inducing guanine alkylation,resulting in GC to AT *** effect is different between clones of a genotype and between genotypes of the same *** review presents the results obtained in recent research,where its effect on plant tissues,callus,and cells in suspension has been *** in the phenotypic expression of somaclonal variants were reported,involving morphology,production of secondary metabolites,changes in metabolic routes of resistance,tolerance to stress,increased seed yield,among *** addition,this review compiles the doses and guidelines to consider before using this mutagen,which can serve as a guide for future trials in deciding the response variables,the type of plant explants and the selection of the study *** lines have allowed plant breeders to have a collection of plants with different characteristics,in places where the cultivar does not have its center of *** is important to note that it is still necessary to continue evaluating the heritability of mutations and their behaviour in the environment where they will be established,in order to obtain new varieties of plants that can be cultivated with uniformity in their genetic response.

关键词： Geneticvariability plantbreeding pointmutation guaninealkylation chemical mutagen

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Swiss pumped hydro storage potential for Germany’s electricity system under high penetration of intermittent renewable energy

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Journal of modern Power Systems and Clean Energy 2016年第4期4卷 542-553页

作者： Aagje J.H.van mEERWIJK Reném.J.BENDERS Alejandro davila-mARTINEZ Gideon A.H.LAUGS IVEm(ESRIG) University of GroningenGroningenThe Netherlands

In order to cut greenhouse-gas emissions and increase energy security,the European Commission stimulates the deployment of intermittent renewable energy sources(IRES) towards *** an electricity system with high shares of IRES implemented in the network,energy balancing like storage is needed to secure grid stability and smooth demand *** hydro storage(PHS) is at this moment the best option for large scale *** has strong ambitions to further develop their PHS sector and become the battery of *** this research,the potential of the Swiss PSH plants is explored,whilst taking inflow into the upper reservoirs of the PHS plants into *** simulate electricity imbalance,Germany is used as a case *** already has a high penetration of IRES and has plans to increase installed IRES *** using an energy planning model(Power Plan),three future scenarios of the German electricity system were designed,each with a different set of IRES installed(solar,mixed and wind).Results show that the Swiss battery ambition offers most benefits to a wind-oriented scenario,reducing both shortages as well as *** inflow in Swiss PHS-reservoirs is of minor importance when looking at security of supply,although it was shown that the solarscenario profits more from inflow in terms of system ***,a potential conflict was observed in the solar-scenario between the need for electricity storage and the storage of natural inflow,resulting in more surpluses in the system when inflow was taken into account.

关键词： Pumped hydro storage(PHS) Local hydrology Germany Switzerland Intermittent renewable energy sources(IRES) Scenarios

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Precipitation analysis of as-cast HK40 steel after isothermal aging

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International Journal of minerals,metallurgy and materials 2017年第10期24卷 1125-1133页

作者： m.L. Saucedo-munoz A. Ortiz-mariscal V.m. Lopez-Hirata J.D. Villegas-Cardenas Orlando Soriano-Vargas Erika O. Avila-davila Instituto Politecnico Nacional (ESIQIE) Apdo. Postal 118-395 Mexico D.F. 07300 Universidad Politecnica Metepec y Tultitlan Edo. Mex. Mexico Tecnológico de Estudios Superiores de Jocotitlan Jocotitlan Edo. Mex. Mexico

As-cast HK40 steel was aged at 700, 800, or 900℃ for times as long as 2000 h. microstructural characterization showed that the primary m_7C_3 carbide network contained a substantial content of manganese, in agreement with the microsegregation of manganese calculated by Thermo-Calc using the Scheil–Gulliver module. The dissolution of primary carbides caused the solute supersaturation of austenite and subsequent precipitation of fine m_(23)C_6 carbides in the austenite matrix for aged specimens. During prolonged aging, the carbide size increased with increasing time because of the coarsening process. A time–temperature–precipitation diagram for m_(23)C_6 carbides was calculated using the Thermo-CalcPRISmA software; this diagram showed good agreement with the experimental growth kinetics of precipitation. The fine carbide precipitation caused an increase in hardness; however, the coarsening process of carbides promoted a decrease in hardness. Nanoindentation tests of the austenite matrix indicated an increase in ductility with increasing aging time.

关键词： as-cast HK40 steel carbide precipitation aging precipitation simulation

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Did maize domestication and early spread mediate the population genetics of corn leafhopper?

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Insect Science 2019年第3期26卷 569-586页

作者： Julio S. Bernal Amanda m. davila-Flores Raul F. medina Yolanda H. Chen Kyle E. Harrison Kimberly A. Berrier Department of Entomology Texas A&M University College Station Texas USA Department of Plant and Soil Sciences University of Vermont Burlington Vermont USA

Investigating how crop domestication and early farming mediated crop attributes, distributions, and interactions with antagonists may shed light on today's agricultural pest problems. Crop domestication generally involved artificial selection for traits desirable to early farmers, for example, in creased productivity or yield, and enhanced qualities, though invariably it altered the interactions between crops and insects, and expanded the geographical ranges of crops. Thus, some studies suggest that with crop domestication and spread, insect populations on wild crop ancestors gave rise to pestiferous insect populations on crops. Here, we addressed whether the emergence of corn leafhopper (Dalbulus ma id is) as an agricultural pest may be associated with domestication and early spread of maize (Zea mays mays). We used AFLP markers and mitochondrial COI sequences to assess population genetic structuring and haplotype relationships among corn leafhopper samples from maize and its wild relative Zea diploperennis from multiple locations in mexico and Argentina. We uncovered seven corn leafhopper haplotypes contained within two haplogroups, one haplogroup containing haplotypes associated with maize and the other containing haplotypes associated with Z. diploperennis in a mountainous habitat. Within the first haplogroup, one haplotype was predominant across mexican locations, and another across Argentinean locations;both were considered pestiferous. We suggested that the divergence times of the maize-associated haplogroup and of the "pestiferous" haplotypes are correlated with the chronology of maize spread following its domestication. Overall, our results support a hypothesis positing that maize domestication favored corn leafhopper genotypes preadapted for exploiting maize so that they became pestiferous, and that with the geographical expansi on of maize farming, corn leafhopper colonized Z. diploperennis, a host exclusive to secluded habitats that serves as a refuge fo

关键词： Dalbulus maidis haplotype network analysis perennial teosinte population genetic structuring Zea diploperennis Zea mays mays

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Effect of homogenization process on the hardness of Zn-Al-Cu alloys

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International Journal of minerals,metallurgy and materials 2015年第10期22卷 1076-1081页

作者： Jose D.Villegas-Cardenas maribel L.Saucedo-munoz Victor m.Lopez-Hirata Antonio De Ita-De la Torre Erika O.Avila-davila Jorge Luis Gonzalez-Velazquez Department of metallurgy and materials Instituto Politecnico Nacional(IPN-ESIQIE)Mexico City 07300Mexico Department of Industrial Engineering Universidad Politecnica del Valle de MexicoMexico CityMexico Department of materials Universidad Autonoma Metropolitana(UAM)Unidad AzcapotzalcoMexico

The effect of a homogenizing treatment on the hardness of as-cast Zn–Al–Cu alloys was investigated. Eight alloy compositions were prepared and homogenized at 350 °C for 180 h, and their Rockwell 'B' hardness was subsequently measured. All the specimens were analyzed by X-ray diffraction and metallographically prepared for observation by optical microscopy and scanning electron microscopy. The results of the present work indicated that the hardness of both alloys(as-cast and homogenized) increased with increasing Al and Cu contents; this increased hardness is likely related to the presence of the θ and τ′ phases. A regression equation was obtained to determine the hardness of the homogenized alloys as a function of their chemical composition and processing parameters, such as homogenization time and temperature, used in their preparation.

关键词： zinc–aluminum–copper alloys casting hardness homogenization phases microstructure

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Dynamic control of high-voltage actuator arrays by light-pattern projection on photoconductive switches

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microsystems & Nanoengineering 2023年第3期9卷 387-396页

作者： Vesna Bacheva Amir Firouzeh Edouard Leroy Aiste Balciunaite Diana davila Israel Gabay Federico Paratore moran Bercovici Herbert Shea Govind Kaigala Faculty of mechanical Engineering Technion-Israel Institute of Technology3200003 HaifaIsrael IBm Research Europe-Zurich Säumerstrasse 48803 RüschlikonSwitzerland Soft Transducers Laboratory(LmTS) Ecole Polytechnique Fédérale de Lausanne(EPFL)2000 NeuchâtelSwitzerland Present address:Laboratory of Soft materials and Interfaces ETH Zürich8093 ZürichSwitzerland Present address:School of Biomedical Engineering Vancouver Prostate CentreLife Sciences InstituteUniversity of British ColumbiaVancouverCanada

The ability to control high-voltage actuator arrays relies, to date, on expensive microelectronic processes or on individual wiring of each actuator to a single off-chip high-voltage switch. Here we present an alternative approach that uses on-chip photoconductive switches together with a light projection system to individually address high-voltage actuators. Each actuator is connected to one or more switches that are nominally OFF unless turned ON using direct light illumination. We selected hydrogenated amorphous silicon (a-Si:H) as our photoconductive material, and we provide a complete characterization of its light to dark conductance, breakdown field, and spectral response. The resulting switches are very robust, and we provide full details of their fabrication processes. We demonstrate that the switches can be integrated into different architectures to support both AC and DC-driven actuators and provide engineering guidelines for their functional design. To demonstrate the versatility of our approach, we demonstrate the use of the photoconductive switches in two distinctly different applications—control of µm-sized gate electrodes for patterning flow fields in a microfluidic chamber and control of cm-sized electrostatic actuators for creating mechanical deformations for haptic displays.

关键词： projection light sized

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In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria

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Journal of Forestry Research 2019年第5期30卷 1765-1777页

作者： Jessica de Cassia Tomasi Juliana Degenhardt-Goldbach Renata Lucia Grunennvaldt Germana davila dos Santos Vania Aparecida Vicente Luziane Franciscon Cleusa Bona marguerite Quoirin Department of Crop Production and Protection Federal University of Parana1540 Rua dos Funciona′riosCuritibaPR 80035-060Brazil Brazilian Agricultural Research Corporation(EmBRAPA)Forestry ColomboPRBrazil Department of Basic Pathology Federal University of ParanaCuritibaBrazil Department of Botany Federal University of ParanaCuritibaBrazil

Yerba mate(Ilex paraguariensis ***.-Hil.)is a species of great economic,social and environmental importance for the southern regions of Brazil,Uruguay and *** the most diverse products are obtained from mate leaves,including mate *** objective of this study was to establish shoot meristem cultures(meristematic dome and a few primordia)of elite clones and identify the endophytic bacteria present in the *** tested the effect of clones(F1,F2,A03 and A07),culture media(mS,1/2mS,1/4mS and WPm),cytokinins(kinetin,BA and 2iP),activated charcoal(1,2 and 3 g L^-1),and disinfecting agent(sodium hypochlorite and mercuric chloride)on in vitro establishment.F1 and F2 clones were the most responsive for shoot meristem in vitro *** medium supplemented with 8.8 lm 2iP,0.2 lm NAA and 3 g L^-1 activated charcoal was the most suitable for the in vitro establishment of the F1 *** phytotoxic effect of the disinfectant was observed and some meristems *** isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium *** conclude,we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response.

关键词： Yerba mate Tissue culture 2iP Activated charcoal Endophytic bacteria Agrobacterium larrymoorei Oxidation

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Candelilla Wax as Natural Slow-Release matrix for Fertilizers Encapsulated by Spray Chilling

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Journal of Renewable materials 2018年第3期6卷 226-236页

作者： Noe Navarro-Guajardo Edgar miguel Garcia-Carrillo Carlos Espinoza-Gonzalez Ruben Tellez-Zablah Fatima davila-Hernandez Jorge Romero-Garcia Antonio Ledezma-Perez Jesus Alfonso mercado-Silva Claudia Anahi Perez Torres Nicolaza Pariona Technological Institute of Saltillo Blvd.Venustiano Carranza 2400C.P.25280SaltilloCoahuilaMéxico Research Center for Applied Chemistry Sonomimetikos Research GroupBlvd.Enrique Reyna Hermosillo 140C.P.25294SaltilloCoahuilaMéxico Research Center for Applied Chemistry National Laboratory of GrapheneBlvd.Enrique Reyna Hermosillo 140C.P.25294SaltilloCoahuilaMéxico CONACyT–Institute of Ecology A.C. Carretera antigua a Coatepec 351El HayaC.P.91070XalapaVeracruzMéxico Network for Advanced molecular Studies Institute of Ecology A.C.Carretera antigua a Coatepec 351El HayaC.P.91070XalapaVeracruzMéxico

Efforts to improve crop yields with efficient use of fertilizers are needed to guarantee global food *** slow-release fertilizer systems(SRFs)encapsulated in biodegradable matrices are being developed to address this global *** a wide range of strategies for SRFs development,we explored a natureinspired solution based on the plant cuticle model and its function as a membrane for water and nutrient transport ***,Candelilla wax,extracted from Candelilla wild plants(Euphorbia antisyphilitica),is studied as a renewable slow-release matrix for fertilizers encapsulated by a modified spray chilling *** this process,microencapsulates containing 40 wt%of phosphorus fertilizer are obtained with distinctive sizes and chemical characteristics,presenting a slow-release *** the abovementioned features,novel insights into fertilizer release mechanisms based on plant cuticle models are discussed.

关键词： Candelilla wax encapsulation phosphorus slow-release fertilizers(SRFs) spray chilling

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Cerebral dopamine neurotrophic factor transfection in dopamine neurons using neurotensin-polyplex nanoparticles reverses 6-hydroxydopamine-induced nigrostriatal neurodegeneration

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Neural Regeneration Research 2022年第4期17卷 854-866页

作者： manuel A.Fernandez-Parrilla David Reyes-Corona Yazmin m.Flores-martinez Rasajna Nadella michael J.Bannon Lourdes Escobedo minerva maldonado-Berny Jaime Santoyo-Salazar Luis O.Soto-Rojas Claudia Luna-Herrera Jose Ayala-davila Juan A.Gonzalez-Barrios Gonzalo Flores maria E.Gutierrez-Castillo Armando JEspadas-Alvarez Irma A.martínez-Dávila Porfirio Nava Daniel martinez-Fong Departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa Institucional de Biomedicina molecular Escuela Nacional de Medicina y HomeopatíaInstituto Politécnico NacionalCiudad de MéxicoMéxico Department of Biosciences IIIT-SrikakulamRajiv Gandhi University of Knowledge Technologies(RGUKT)Andhra PradeshIndia Department of Pharmacology Wayne State University School of MedicineDetroitMIUSA Departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de MéxicoTlalnepantla de BazEdo.de MéxicoMéxico Departamento de Fisiología Escuela Nacional de Ciencias BiológicasCiudad de MéxicoMéxico Laboratorio de medicina Genómica Hospital Regional“1°de Octubre”ISSSTECiudad de MéxicoMéxico Laboratorio de Neuropsiquiatría Instituto de FisiologíaBenemérita Universidad Autónoma de PueblaPueblaPueblaMéxico Departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Programa de Nanociencias y nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Overexpression of neurotrophic factors in nigral dopamine neurons is a promising approach to reverse neurodegeneration of the nigrostriatal dopamine system,a hallmark in Parkinson's *** human cerebral dopamine neurotrophic factor(h CDNF)has recently emerged as a strong candidate for Parkinson's disease *** study shows that h CDNF expression in dopamine neurons using the neurotensinpolyplex nanoparticle system reverses 6-hydroxydopamine-induced morphological,biochemical,and behavioral *** independent electron microscopy techniques showed that the neurotensin-polyplex nanoparticles containing the h CDNF gene,ranging in size from 20 to 150 nm,enabled the expression of a secretable h CDNF in *** injection in the substantia nigra compacta on day 21 after the 6-hydroxydopamine lesion resulted in detectable h CDNF in dopamine neurons,whose levels remained constant throughout the study in the substantia nigra compacta and *** with the lesioned group,tyrosine hydroxylase-positive(TH^(+))nigral cell population and TH+fiber density rose in the substantia nigra compacta and striatum after h CDNF *** increase inβIII-tubulin and growth-associated protein 43 phospho-S41(GAP43 p)followed TH^(+)cell recovery,as well as dopamine and its catabolite *** reversal(80%)of drugactivated circling behavior and full recovery of spontaneous motor and non-motor behavior were ***-derived neurotrophic factor recovery in dopamine neurons that also occurred suggests its participation in the neurotrophic *** findings support the potential of nanoparticle-mediated h CDNF gene delivery to develop a disease-modifying treatment against Parkinson's *** Institutional Animal Care and Use Committee of Centro de Investigación y de Estudios Avanzados approved our experimental procedures for animal use(authorization No.162-15)on June 9,2019.

关键词： axonal growth brain-derived neurotrophic factor gene delivery nanoparticles neuritogenesis neuronal cytoskeleton neuroregeneration neurorestoration neurotrophic therapy Parkinson's disease reinnervation substantia nigra

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