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Calcium silicate bioactive ceramics induce osteogenesis through oncostatin M

Bioactive Materials 2021年第3期6卷 810-822页

作者： Panyu Zhou Demeng Xia Zhexin Ni Tianle Ou Yang Wang Hongyue Zhang Lixia Mao Kaili Lin Shuogui Xu Jiaqiang Liu Department of Emergency Changhai HospitalNaval Medical UniversityShanghaiChina Department of Gynecology of Traditional Chinese Medicine Changhai HospitalNaval Medical UniversityShanghaiChina Department of Clinical Medicine the Naval Medical UniversityShanghaiChina Department of Oral&Cranio-Maxillofacial Surgery Shanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineShanghai Key Laboratory of StomatologyShanghai Research Institute of StomatologyShanghaiChina

Immune reactions are a key factor in determining the destiny of bone substitute materials after implantation.Macrophages,the most vital factor in the immune response affecting implants,are critical in bone formation,as well as bone biomaterial-mediated bone repair.Therefore,it is critical to design materials with osteoimmunomodulatory properties to reduce host-to-material inflammatory responses by inducing macrophage polarization.Our previous study showed that calcium silicate(CS)bioceramics could significantly promote osteogenesis.Herein,we further investigated the effects of CS on the behavior of macrophages and how macrophages regulated osteogenesis.Under CS extract stimulation,the macrophage phenotype was converted to the M2 extreme.Stimulation by a macrophage-conditioned medium that was pretreated by CS extracts resulted in a significant enhancement of osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs),indicating the important role of macrophage polarization in biomaterial-induced osteogenesis.Mechanistically,oncostatin M(OSM)in the macrophage-conditioned medium promoted osteogenic differentiation of BMSCs through the ERK1/2 and JAK3 pathways.This in vivo study further demonstrated that CS bioceramics could stimulate osteogenesis better thanβ-TCP implants by accelerating new bone formation at defective sites in the femur.These findings improve our understanding of immune modulation of CS bioactive ceramics and facilitate strategies to improve the in vitro osteogenesis capability of bone substitute materials.

关键词： Osteoimmune Osteogenesis Calcium silicate Macrophage Macrophage polarization

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Getting insights into gas-phase sulfation effect on catalytic performance of praseodymium oxides in NH_(3)-SCR of NO

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Journal of Rare Earths 2023年第6期41卷 952-958,I0005页

作者： Yandi Cai Peng Yang Qinglong Liu kaili Ma Wenxin Ma Wang Song Qiuhui Qian Fei Gao Wei Tan lin Dong State Key Laboratory of Pollution Control and Resource Reuse School of EnvironmentJiangsu Key Laboratory of Vehicle Emissions ControlCenter of Modern AnalysisKey Laboratory of Mesoscopic Chemistry of MOESchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Analysis and Testing Center Southeast UniversityNanjing 211189China National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology School of Environmental Science and EngineeringSuzhou University of Science and TechnologySuzhou 215009China

Sulfation treatment has been widely used to promote the catalytic performance of ceria(CeO_(2))based catalysts for the selective catalytic reduction of NO by NH_(3)(NH_(3)-SCR of NO).Praseodymium oxide(PrO_(x)),another commonly used rare earth material with similar structural properties as CeO_(2),also shows satistactory redox properties due to the facile redox cycle of Pr^(3+)■Pr^(4+).In this work,gas phase sulfation treatment with varied duration was performed on PrO_(x) at 200℃,and the NH_(3)-SCR activity of sulfated PrO_(x) was evaluated.Based on the results of systematic characterizations(e.g.,N_(2)-physisorption,NH_(3) oxidation,NO oxidation,in situ diffuse Fourier transform infrared spectroscopy),it is revealed that the catalytic performance of sulfated PrO_(x)is highly dependent on the sulfation time(or the amount of sulfate species deposited on PrO_(x)),which has a significant impact on the competitive reaction between NH_(3) oxidation and NH_(3)-SCR of NO,thus determining the NH_(3)-SCR activity of PrO_(x).This work provides new insight into tuning the interaction between PrO_(x) surface and reactants(NO,NH_(3))via sulfation treatment,which cam guide the design and application of PrO_(x)based catalysts for NH_(3)-SCR of NO in the future.

关键词： NH_(3)-SCR Praseodymium oxides Sulfation treatment Surface acidity NH_(3)oxidation Rare earths

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Development and challenges of cells-and materials-based tooth regeneration

Engineered Regeneration

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Engineered Regeneration 2022年第2期3卷 163-181页

作者： Zeyu Fu Yu Zhuang Jinjie Cui Ruilong Sheng Helena Tomás João Rodrigues Bin Zhao Xudong Wang Kaili Lin Department of Oral and Cranio-Maxillofacial Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyResearch Unit of Oral and Maxillofacial Regenerative MedicineChinese Academy of Medical SciencesShanghai 200011China School of Materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China CQM-Centro de Quimica da Madeira MMRGUniversidade da MadeiraCampus da Penteada9000-390 FunchalMadeiraPortugal School of Materials Science and Engineering Center for Nano Energy MaterialsNorthwestern Polytechnical UniversityXi’an710072China

Tooth defect and loss are common clinical diseases in stomatology.With the extension of life expectancy,there is an increasing demand for tooth tissue and whole tooth regeneration.Compared with traditional oral prosthetic treatment,tooth regeneration has unique advantages and has become one of the hotspots towards oral biomedical treatment.In this review,we discussed the development and challenges of tooth regeneration based on cells and materials,including tooth enamel,dentin,dental pulp,cementum,dentin-pulp complex,and the whole tooth regeneration,in order to provide a comprehensive,up-to-date,illustrative overview of tooth regeneration issues.The mechanisms of the regeneration were also summarized and discussed.Moreover,this review hints the future perspective and research direction of tooth regeneration in the challenging field of regenerative dentistry.

关键词： Tooth regeneration Cells Materials Regenerative dentistry

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Coarsening kinetics of γ' precipitates in a Re-containing Ni-based single crystal superalloy during long-term aging

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Journal of Materials Science & Technology 2021年第3期62卷 1-10页

作者： Jiachen Zhang lin Liu Taiwen Huang Jia Chen kaili Cao Xinxin Liu Jun Zhang Hengzhi Fu State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China

The morphological evolution and coarsening kinetics ofγ'precipitates in a Re-containing Ni-based single crystal superalloy were investigated during isothermal aging at 900,950 and 1000℃.After heat treatment,well-defined cuboidalγ'precipitates with low misfit was obtained within the experimental alloy.Then coarsening rate constants and particle size distribution(PSD)ofγ'phases were calculated and specified based on the measured precipitate sizes for va rying periods of aging times from 100 to 2000 h.After aging for 2000 h,γ'precipitates maintained cubical shape at 900℃,while exhibited sphere at 950 and 1000℃.Coarsening models based on diffusion-controlled process with a functional relationship of r^(3) vs.t(classic Lifshitz-Slyozov-Wagner coarsening model)and interface-controlled model with a function of r^(2) vs.t(trans-interface diffusion-controlled coarsening model)were investigated to fit between the experimental results and theoretical analysis.It was found that Re as the slowest diffusing solute in the alloy constituted the rate-limited step for coarsening based on LSW model,while the process limiting coarsening as governed by an interface diffusion process could possibly be related to the Al diffusion through theγ/γ'interface.The PSDs and coarsening exponent were discussed by comparing the experimental data with predictions of LSW and TIDC models.Finally,coarsening mechanism could be divided into four regimes:(i)coarsening by diffusion-controlled;(ii)coarsening by diffusion and interface cocontrolled;(iii)coarsening by interface-controlled;(iv)coarsening by interface-controlled accompanied withγ'coalescence.

关键词： Precipitate coarsening Three-dimensional atom probe(3DAP) Interfacial energy Diffusion Particle size distribution Ni-base single crystal superalloys

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多细胞构建血管化组织工程骨在骨修复中的应用

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中国组织工程研究 2022年第27期26卷 4386-4392页

作者：赵豆豆林开利上海理工大学材料科学与工程学院上海市200093 上海交通大学医学院附属第九人民医院上海市200011

背景:组织工程骨构建体的血管化性能不足是限制骨组织工程用于修复大尺寸骨缺损临床应用的主要挑战。目的:对近年来利用骨形成细胞和血管生成细胞构建组织工程构建体(基于支架或无支架)在骨修复中的应用进行了概述,以期实现组织工程骨... 详细信息

背景:组织工程骨构建体的血管化性能不足是限制骨组织工程用于修复大尺寸骨缺损临床应用的主要挑战。目的:对近年来利用骨形成细胞和血管生成细胞构建组织工程构建体(基于支架或无支架)在骨修复中的应用进行了概述,以期实现组织工程骨可持续的血管生成及生成功能完善的血管,从而提高骨组织工程在大尺寸骨缺损修复应用中的细胞存活率、并为促进骨的形成和重塑提供参考。方法:应用计算机对中国知网、PubMed及Web of Science数据库2000-2021年发表的文献进行了检索,中文检索词为“骨组织工程、成骨成血管、多细胞”,英文检索词为“Bone Tissue Engineering,Osteogenesis and angiogenesis,Multicellular”,根据纳入和排除标准,最终纳入63篇文献进行结果分析。结果与结论:①目前最常用的骨形成细胞主要有间充质干细胞、脂肪干细胞和成骨细胞,常用的血管生成细胞有人脐静脉内皮细胞和内皮祖细胞。②支架内包封细胞在实现各类细胞的精准定位方面优于在支架上接种细胞。③将两种单一细胞膜片相叠加或者共培养单层细胞膜片相叠加的方法,可以调控各类细胞的位置,从而构建血管化网络。④目前,基于支架的组织工程技术还需克服支架降解速率与组织再生速率的不匹配性、细胞与生物材料相互作用不可控等问题,而基于无支架细胞膜片的组织工程还需要克服力学强度低的难题。⑤未来研究需构建具有临床所需大小的功能性3D血管化组织工程骨,还需关注包括细胞培养和生物学作用机制;结合组织工程、细胞工程和基因工程构建组织工程骨,有望实现刺激早期血管生成、保持血液循环,防止构建体内部的细胞死亡,并起到加速构建体修复临床大体积骨缺损的作用。

关键词：血管化骨组织工程多细胞共培养成骨成血管支架细胞膜片综述

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Influence of Hardcopy Types on Extraction Quality of Holographic Watermark

Influence of Hardcopy Types on Extraction Quality of Hologra...

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第30届中国控制与决策会议

作者： XIE Yong LIU lin WANG kaili Hunan University of Technology

In this paper, firstly the high-frequency coefficient of Fourier hologram watermark was embedded into the middle-frequency of the host image subject to wavelet transform for twice. Then four common hardcopy types（inkjet, laser, offset printing and digital printing） were used to output watermarked images. Finally the watermark extraction quality of the hardcopy images was evaluated from paper surface quality, color space conversions of images and output resolution by using NC parameters. Experiment results showed that the watermark extraction qualities between digital image, presswork and printing are very different, and the rank is: inkjet printing ＞ offset printing ＞ digital printing and laser printing. The printed substrate paper with higher whiteness and gloss is beneficial to watermark extraction. The color space conversions of watermarked image in input and output process affected color distribution and image reproduction quality of original image, then the watermark extraction quality was influenced. The hardcopy resolution has little effect on watermark extraction quality on the premise of satisfying the image reproduction quality. Therefore, actual hardcopy type should be used instead of simulated attacks or desktop printing when studying the anti-print/scan robustness of watermarking algorithm. The substrate paper with high surface quality facilitate the improvement of watermark extraction quality. And the resolution is appropriate as long as the output reproduction quality is satisfied when verifying the anti-print/scan robustness of watermark scheme.

关键词： Hardcopy style Watermark extraction quality Paper printability Color space conversions Output resolution

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Visualizing nonlinear resonance in nanomechanical systems via single-electron tunneling

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Nano Research 2021年第4期14卷 1156-1161页

作者： Xinhe Wang lin Cong Dong Zhu Zi Yuan Xiaoyang lin Weisheng Zhao Zaiqiao Bai Wenjie Liang Ximing Sun Guang-Wei Deng kaili Jiang Fert Beijing Research Institute School of Microelectronics&Beijing Advanced Innovation Centre for Big Data and Brain Computing(BDBC)Beihang UniversityBeijing 100191China State Key Laboratory of Low-Dimensional Quantum Physics Department of Physics and Tsinghua-Foxconn Nanotechnology Research CenterTsinghua UniversityBeijing 100084China Key Laboratory of Quantum Information CASUniversity of Science and Technology of ChinaHefei 230026China Department of Physics Beijing Normal UniversityBeijing 100875China Institute of Physics Chinese Academy of SciencesBeijing 100080China Institute of Nuclear and New Energy Technology Collaborative Innovation Center of Advanced Nuclear Energy TechnologyKey Laboratory of Advanced Reactor EngineeringTsinghua UniversityBeijing 100084China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054China

Numerous reports have elucidated the importance of mechanical resonators comprising quantum-dot-embedded carbon nanotubes(CNTs)for studying the effects of single-electron transport.However,there is a need to investigate the single-electron transport that drives a large amplitude into a nonlinear regime.Herein,a CNT hybrid device has been investigated,which comprises a gate-defined quantum dot that is embedded into a mechanical resonator under strong actuation conditions.The Coulomb peak positions synchronously oscillate with the mechanical vibrations,enabling a single-electron Chopper*1 mode.Conversely,the vibration amplitude of the CNT versus its frequency can be directly visualized via detecting the time-averaged single-electron tunneling current.To understand this phenomenon,a general formula is derived for this time-averaged single-electron tunneling current,which agrees well with the experimental results.By using this visualization method,a variety of nonlinear motions of a CNT mechanical oscillator have been directly recorded,such as Duffing nonlinearity,parametric resonance,and double-,fractional-,mixed-frequency excitations.This approach opens up burgeoning opportunities for investigating and understanding the nonlinear motion of a nanomechanical system and its interactions with electron transport in quantum regimes.

关键词： carbon nanotube mechanical resonator quantum dot nonlinear coupling

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A correlative multidimensional study ofγ’precipitates with Ta addition in Re-containing Ni-based single crystal superalloys

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Journal of Materials Science & Technology 2021年第16期75卷 68-77页

作者： Jiachen Zhang Taiwen Huang kaili Cao Jia Chen Huajing Zong Dong Wang Jian Zhang Jun Zhang lin Liu State Key Laboratory of Solidification Processing Northwestern Poly technical UniversityXi'an710072PR China Shenyang National Laboratory for Materials Science Institute of Metal ResearchShenyang110016PR China

The microstructural evolution in Re-containing Ni-based single crystal superalloys with different Tantalum(Ta)content(2 Ta,5 Ta and 8 Ta in wt%)was investigated.Ta addition significantly affected theγ’precipitate morphology,γ/γ’lattice misfit and microstructural stability during long-term aging.Results showed that the partitioning behaviors of solutes were enhanced by Ta addition,meanwhile,the reversal partitioning behavior of W was triggered which partitioned fromγ’precipitate to matrix.The elemental concentration redistribution caused variations in lattice misfit from positive to negative,the values of lattice misfit were measured to be 0.16%for 2 Ta alloy,then decreased to-0.07%for 5 Ta alloy and negatively increased to-0.23%for 8 Ta alloy.These variations in the lattice misfit were reflected on the transition ofγ’morphology from round-cornered cuboidal shape to cuboidal with sharp corners,accomplished with increasing shape parameter ratioη.Consequently,the optimalγ’shape could be obtained at lattice misfit of approximately 0.3%.Theγ’coarsening investigation at 900℃(up to 2000 h)indicated that Ta addition was beneficial for improving the microstructural stability by reducing the coarsening rate and interfacial energy,accompanied by the enhanced capability of resistingγ’coalescence.By incorporating the calculated interfacial energy,computational modeling,Thermo-Calc and PrecipiCalc,were employed to elucidate theγ’kinetic pathways,the simulation results agreed with experiments,indicating that the model and parameters were reasonable.Additionally,it was found that there was no overlap betweenγ’nucleation and coarsening when theγ/γ’interfacial energy increased to a critical value.

关键词： Tantalum Ni-based superalloys Atom probe tomography Lattice misfit Coarsening kinetics

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Direct discrimination between semiconducting and metallic single-walled carbon nanotubes with high spatial resolution by SEM

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Nano Research 2017年第6期10卷 1896-1902页

作者： Dongqi Li Yang Wei Jin Zhang Jiangtao Wang Yinghong lin Peng Liu Shoushan Fan kaili Jiang State Key Laboratory of Low-Dimensional Quantum Physics Department of Physics and Tsinghua-Foxconn Nanotechnology Research Center Tsinghua University Beijing 100084 China Collaborative Innovation Center of Quantum Matter Beijing 100084 China FEI Company Building No. 8 No. 399 Shengxia Road Pudong Shanghai 201210 China

Single-walled carbon nanotube （SWCNT） films with a high density exhibit broad functionality and great potential in nanodevices, as SWCNTs can be either metallic or semiconducting in behavior. The films greatly benefit from characterization technologies that can efficiently identify and group SWCNTs based on metallic or semiconducting natures with high spatial resolution. Here, we developed a facile imaging technique using scanning electron microscopy （SEM） to discriminate between semiconducting and metallic SWCNTs based on black and white colors. The average width of the single-SWCNT image was reduced to -9 nm, -1/5 of previous imaging results. These achievements were attributed to reduced surface charging on the SiOdSi substrate under enhanced accelerating voltages. With this identification technique, a CNT transistor with an on/off ratio of 〉10s was fabricated by identifying and etching out the white metallic SWCNTs. This improved SEM imaging technique can be widely applied in evaluating the selective growth and sorting of SWCNTs.

关键词： single-walled carbonnanotube,scanning electronmicroscopy,surface charging,transistor

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SEM imaging of insulating specimen through a transparent conducting veil of carbon nanotube

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Nano Research 2022年第7期15卷 6407-6415页

作者： Xinyu Gao Guo Chen He Ma Yuchen Ju Ke Zhang lin Cong Wen Ning Zi Yuan Zebin Liu lina Zhang Peng Liu Shoushan Fan kaili Jiang State Key Laboratory of Low-Dimensional Quantum Physics Department of Physics and Tsinghua-Foxconn Nanotechnology Research CenterTsinghua UniversityBeijing 100084China College of Applied Sciences Beijing University of TechnologyBeijing 100124China Frontier Science Center for Quantum Information Beijing 100084China Beijing Advanced Innovation Center for Future Chips Beijing 100084China

Observing the morphology of insulating specimen in scanning electron microscope(SEM)is of great significance for the nanoscale semiconductor devices and biological tissues.However,the charging effect will cause image distortion and abnormal contrast when observing insulating specimen in SEM.A typical solution to this problem is using metal coating or water-removable conductive coating.Unfortunately,in both cases the surface of the specimen is covered by a thin layer of conductive material which hides the real surface morphology and is very difficult to be completely removed after imaging.Here we show a convenient,residue-free,and versatile method to observe real surface morphology of insulating specimen without charging effect in SEM with the help of a nanometer-thick film of super-aligned carbon nanotube(SACNT).This thin layer of SACNT film,like metal,can conduct the surface charge on insulating specimen through the sample stage to the ground,thus eliminating the charging effect.SACNT film can also be used as the conductive tape to carry and immobilize insulating powder or particles during SEM imaging.Different from the metal coating,SACNT film is transparent,so that the real microstructure of the insulating specimen surface can be observed.In addition,SACNT film can be easily attached to and peeled off from the surface of specimen without any residue.This convenient,residue-free,and versatile method can open up new possibilities in nondestructive SEM imaging of a wide variety of insulating materials,semiconductor devices,and biological tissues.

关键词： insulating specimen scanning electron microscope(SEM) super-aligned carbon nanotube(SACNT)film real morphology residue-free non-destructive imaging

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