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Promotion on the differentiation of retinal Müller cells into retinal ganglion cells by Brn-3b

International Journal of Ophthalmology(English edition) 2016年第7期9卷 948-954页

作者： Zhen-kai Wu Lan Cao Xue-Yong Zhang Wei-Tao Song xiao-Bo xia Department of Ophthalmology Xiangya HospitalCentral South UniversityChangsha 410008 Hunan ProvinceChina

AIM： To investigate the role of Brn-3b in differentiation process of stem cells derived from retinal Muller cells into the ganglion cell. METHODS： The passage culture method of Muller cells from retina of newborn Sprague Dawley rats was carried out by repeated incomplete pancreatic enzyme digestion method. The cells were detected by fluorescence- activated ceil sorter （FACS）, immunohistochemistry technology and reverse transcription-polymerase chain reaction （RT-PCR） to determine the purity. The third passage of cells was induced in the serum-free dedifferentiation medium. The expression of the specific markers Ki-67 and nestin of retinal stem cells was measured by RT-PCR and Western blot. The cell proliferation of retinal stem cells was detected by 5- Ethynyl-2＇-deoxyuridine （Edu） staining. The cells were randomly divided into 5 groups as follows： group A： Brn-3bsiRNA group; group B： Brn-3b control siRNA group; group C： pGC-Brn-3b-green fluorescent protein （GFP） group; group D： pGC-GFP group; group E： control group （without any handling）. The purified Muller cells were cultured for 3-7d, then, the percentage of ganglion cells was counted by immunofluorescence staining. RESULTS： FACS demonstrated the purity of retinal Muller cells was more 97.44%. A few spherical cell spheres appeared. Immunofluorescence staining showed that stem cells within the spheres were positive for retinal stem cell-specific markers nestin （red fluorescence, 92.94%±6.48%） and Ki-67 （green fluorescence, 85.96%± 6.04% ）. Meanwhile, RT-PCR analysis showed cell spheres in the culture to have expressed a battery of transcripts characteristic of stem cells such as nestin and Ki-67, which were absent in the Muller cells. Western blot analysis further confirmed the expression of nestin and Ki-67 in the cell spheres but not in the Muller cells. Edu staining showed most of the nuclei within the cell spheres were stained red （82.80%±6.65%）, suggesting the new cell spheres had the capacity for effectiv

关键词： Mtiller cells retinal ganglion cells Brn-3b stem cells differentiation

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Electrochemiluminescence of 1,8-Naphthalimide-Modified Carbon Nitride for Cu^(2+) Detection

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Journal of Analysis and Testing 2022年第3期6卷 296-307页

作者： Feng-Yu Liu Tong-kai Zhang Yi-Long Zhao Hong-xia Ning Fu-Sheng Li State Key Laboratory of Fine Chemicals School of ChemistryDalian University of TechnologyDalian 116024China State Key Laboratory of Fine Chemicals Institute of Artif cial PhotosynthesisDUT-KTH Joint Education and Research Centre on Molecular DevicesDalian University of TechnologyDalian 116024China

It is essential to screen novel luminophores and co-reactants for the development of electrochemiluminescence(ECL)*** this work,a series of 1,8-naphthalimide-modified graphitic carbon nitride(CN/1,8-NDI_(x))ECL luminophores were successfully developed for the quantitative determination of Cu^(2+)with K_(2)S_(2)O_(8)as a *** CN/1,8-NDI_(x)was immobilized on the surface of the glassy carbon electrode(GCE),a strong ECL signal could be produced(at a potential of-1.3 V ***/AgCl),which was threefold stronger than that of unmodified g-C_(3)N_(4).The intensity of ECL could be linearly quenched by Cu^(2+)over a concentration range from 5×10^(-9)to 1×10^(-4)mol/L,and with a low limit of detection(LOD)of0.86 nmol/*** results may provide a new perspective on the novel ECL platforms development.

关键词： Electrochemiluminescence Carbon nitride Quantification Cu^(2+)ion detection

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IONIC CONDUCTIVITY AND CATALYTIC PERFORMANCE OF A2BO4/La2O3 CATALYSTS FOR OXIDATIVE COUPLING OF METHANE

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Journal of Energy Chemistry 1993年第3期 203-211页

作者： BI Ying-Li xia xin-Rui LI Li-Tao ZHEN kai-Ji Department of Chemistry, Jilin University Changchun, Jilin 130023

A series of La2O3cartalysts modified by A2BO4compounds with supe-rionic conductivity were prepared. It was observed that the addition of A2BO4compounds to La2O3enhances C2yields, and the C2yields over the modified cat-alysts are intimately related with their conductivities. The effects of A2BO4compounds on the conductivity and catalytic performance were discussed. Onlywhen ion A has high conductivity, can the catalyst show good catalytic perfor-mance. A mechanism for interpreting the significant effects of alkali metals wasproposed.

关键词： IONIC CONDUCTIVITY AND CATALYTIC PERFORMANCE OF A2BO4/La2O3 CATALYSTS FOR OXIDATIVE COUPLING OF METHANE BO La

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Rehabilitation and pharmacotherapy of neuromyelitis optica spectrum disorder:A case report

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World Journal of Clinical Cases 2021年第16期9卷 3951-3959页

作者： xiao-Ju Wang Peng xia Ting Yang kai Cheng An-Liang Chen Xue-Ping Li Department of Rehabilitation Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006Jiangsu ProvinceChina

BACKGROUND Neuromyelitis optica spectrum disorder(NMOSD)is a demyelinating autoimmune disease that affects the central nervous *** typically manifests as optic neuritis or extensive longitudinal myelitis,with or without the presence of anti-aquaporin protein 4 autoantibodies(immunoglobulin G).CASE SUMMARY We report the case of a 45-year-old woman with a history of Sjogren's syndrome who was diagnosed with NMOSD accompanied by spinal cord injury and left calf intermuscular vein *** patient received hormone shock and gamma globulin therapy in the acute phase and standard rehabilitation treatment during *** discharge,the patient was able to control urination and defecation,stand independently,and walk short distances with the aid of a *** This case suggests that pharmacotherapy and standard rehabilitation treatment can improve the prognosis of NMSOD patients.

关键词： Neuromyelitis optica spectrum disorder Spinal cord injury Pharmacotherapy Rehabilitation Sjogren's syndrome Case report

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Zwitterionic Matrix with Highly Delocalized Anionic Structure as an Efficient Lithium Ion Conductor

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CCS Chemistry 2023年第7期5卷 1612-1622页

作者： Shuaishuai Yan Yang Lu Fengxiang Liu Yingchun xia Qiao Li kai Liu State Key Laboratory of Chemical Engineering Department of Chemical EngineeringTsinghua UniversityBeijing 100084

The leakage and volatilization of liquid electrolytes raise potential safety risks in the development of electrochemical energy storage devices with high energy ***,novel solid-state zwitterionic materials containing sulfonyl imide as a highly delocalized anionic structure were synthesized for highly targeted lithium ion conducting *** influences of the molecular structure characteristics on thermal behavior and electrochemical property were investigated *** to the weak Coulomb interaction between the sulfonyl imide moiety and cationic species,the rationally designed zwitterionic electrolytes showed a high conductivity of 0.44 mS cm^(-1).And the obtained high lithium ion transference number of 0.43 is four times higher than that of the widely employed sulfonate ***,excellent cycling stability of the lithium plating/stripping process and super resistance to electrochemical oxidation(up to 5.5 V)were *** molecular engineering strategy for lithium ion conductor advances new possibilities for developing solvent-free and non-migrating electrolyte matrix materials for lithium metal batteries.

关键词： zwitterion ion conductor energy storage electrolyte lithium battery

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Cavitation erosion behavior of CLAM steel weld joint in liquid lead-bismuth eutectic alloy

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Journal of Iron and Steel Research International 2017年第9期24卷 935-942页

作者： Yu-cheng Lei xiao-kai Guo Hong-xia Chang Tian-qing Li Qiang Zhu Gang Chen Long-ren xiao School of Material Science and Engineering Jiangsu University State Key Laboratory of Advanced Welding and Joining Harbin Institute of Technology

The cavitation erosion of weld joint and base metal of China low activation martensitic（CLAM）steel in liquid lead-bismuth eutectic alloy（LBE）at 550°C was investigated to simulate the cavitation erosion of the first wall and the nuclear main pump impeller in the accelerator driven sub-critical system（ADS）.A suit of ultrasonic cavitation facility was self-designed to study the cavitation *** studying the surface micro topography,roughness and mean pit depth of the tested specimens,it was found that some crater clusters and large scale cracks appeared on the tested specimen surface after the formation of numerous single craters,and the base metal exhibited much better cavitation erosion resistance than the weld bead due to the difference in their mechanical properties and *** addition,by comparing the results of static corrosion and cavitation erosion,it could be concluded that the cavitation erosion and the dissolution and oxidation corrosion in liquid LBE would accelerate mutually.

关键词： Cavitation erosion CLAM steel Lead-bismuth eutectic alloy Weld joint Dissolution Oxidation

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Anti-N-methyl-D-aspartate receptor encephalitis that aggravates after acinetobacter baumannii pneumonia:A case report

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World Journal of Clinical Cases 2017年第9期5卷 368-372页

作者： Cheng C Wang Da J Li Yi Q xia kai Liu Center of Infectious Diseases West China Hospital of Sichuan University Emergency Department West China Hospital of Sichuan University

We report an atypical case of anti-N-methyl-D-aspartate receptor encephalitis(ANMDARE). A 27-year-old man diagnosed with ANMDARE received immunotherapy and had a good recovery. However, within one month, he developed severe status epilepticus and decreased level of conscience with new hyperpyrexia and dyspnea, and was admitted to the emergency intensive care unit. Acinetobacter baumanii were found in the sputum culture; and anti-NMDAR antibodies were positive(titer: 1/80) in the cerebrospinal fluid. Repeated immunotherapy was administered with antibacterial agents, and the patient recovered except for mild psychiatric sequelae. This is the first report of ANMDARE that aggravates after acinetobacter baumannii pneumonia. Awareness and knowledge of this disorder should be extended, especially in the emergency medicine community.

关键词： Anti-N-methyl-D-aspartate receptor encephalitis Acinetobacter baumannii pneumonia Emergency

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Calculation of Circumferential Modal Frequencies of Permanent Magnet Synchronous Motor

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Journal of Harbin Institute of Technology(New Series) 2019年第1期26卷 77-87页

作者： Shenbo Yu Shuangshuang Zhong Haining Zhao Pengpeng xia kai Guo School of Mechanical Engineering Shenyang University of Technology

Accurate calculation of the natural frequencies of permanent magnet synchronous motors( PMSMs) is essential to the prediction of vibration and noise of PMSMs. The analytical and numerical methods are respectively used to calculate natural frequencies of stator yoke,stator core,stator core with windings,motor casing and stator system. It is found that the relative errors between calculated results with traditional analytical formulas and numerical results are very large. For the sake of reducing relative errors,novel analytical formulas of calculating circumferential modal frequencies of stator core,stator core with windings,motor casing and stator system are proposed. Compared with the results of numerical and experimental modal analysis,the effectiveness of novel analytical formulas is verified.

关键词： natural frequency permanent magnet synchronous motor analytical method numerical method experimental method

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Modulating reaction pathways of formic acid oxidation for optimized electrocatalytic performance of PtAu/CoNC

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Nano Research 2022年第2期15卷 1221-1229页

作者： Mengchao Liang Tianyu xia Han Gao kai Zhao Tianqi Cao Meng Deng xiaoyan Ren Shunfang Li Haizhong Guo Rongming Wang Key Laboratory of Material Physics Ministry of EducationSchool of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China Collaborative Innovation Center of Light Manipulations and Applications Shandong Normal UniversityJinanShandong 250358China Beijing Advanced Innovation Center for Materials Genome Engineering Beijing Key Laboratory for Magneto-Photoelectrical Composite and Interface ScienceSchool of Mathematics and PhysicsUniversity of Science and Technology BeijingBeijing 100083China

Formic acid oxidation(FAO)is a typical anode reaction in fuel cells that can be facilitated by modulating its direct and indirect reaction ***,PtAu bimetallic nanoparticles loaded onto Co and N co-doping carbon nanoframes(CoNC NFs)were designed to improve the selectivity of the direct reaction pathway for efficient *** on these subtle nanomaterials,the influences of elemental composition and carbon-support materials on the two pathways of FAO were investigated in *** results of fuel cell tests verified that the appropriate amount of Au in PtAu/CoNC can promote a direct reaction pathway for FAO,which is crucial for enhancing the oxidation efficiency of formic *** particular,the obtained PtAu/CoNC with an optimal Pt/Au atomic ratio of 1:1(PtAu/CoNC-3)manifests the best catalytic performance among the analogous obtained Pt-based *** FAO mass activity of the PtAu/CoNC-3 sample reached 0.88 A·mg_(Pt)^(-1),which is 26.0 times higher than that of Pt/*** results of first-principles calculation and CO stripping jointly demonstrate that the CO adsorption of PtAu/CoNC is considerably lower than that of Pt/CoNC and PtAu/C,which indicates that the synergistic effect of Pt,Au,and CoNC NFs is critical for the resistance of Pt to CO *** work is of great significance for a deeper understanding of the oxidation mechanism of formic acid and provides a feasible and promising strategy for enhancing the catalytic performance of the catalyst by improving the direct reaction pathway for FAO.

关键词： formic acid oxidation direct oxidation pathway metal organic framework co-doping CO binding energy

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Association of XRCC1 gene polymorphisms with idiopathic azoospermia in a Chinese population

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Asian Journal of Andrology 2007年第6期9卷 781-786页

作者： Ai-Hua Gu, Jie Liang, Ning-xia Lu, Bin Wu, Yan-kai xia, Chun-Cheng Lu, Lin Song, Shou-Lin Wang, xin-Ru WangAi-Hua Gu Jie Liang Ning-xia Lu Bin Wu Yan-kai xia Chun-Cheng Lu Lin Song Shou-Lin Wang xin-Ru Wang Jiangsu Key Laboratory of Reproductive Medicine Institute of Toxicology Nanjing Medical University Nanjing 210029 China

Aim： To assess the possible role of genetic polymorphisms in DNA repair gene XRCC 1 （X-ray repair cross-comple- menting group l） during spermatogenesis by investigating the associations of one promoter polymorphism （T-77C） and two exonic polymorphisms （Argl94Trp and Arg399Gln） in XRCC1 gene with risk of idiopathic azoospermia in a Chinese population. Methods： The genotype and allele frequencies of three observed polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism based on a Chinese population consisting of 171 idiopathic azoospermia subjects and 247 normal-spermatogenesis controls. Results： In our study, all the observed genotype frequencies were in agreement with Hardy-Weinberg equilibrium. The 399A （GA＋AA） allele frequency for idiopathic azoospermia subjects and controls was 0.216 and 0.269, respectively. Compared with GG genotype, the AA genotype of Arg399Gln showed a significant association with a decreased risk of idiopathic azoospermia （odds ratio = 0.315; 95% confidence interval = 0.12-0.86）. However, no significant differences were found between the cases and controls for T-77C and Arg194Trp polymorphisms. The major haplotypes of XRCC1 gene were TCG, TrG and TCA, whereas no haplotypes appeared to be significantly associated with idiopathic azoospermia based on the cutoff of P 〈 0.05. Conclusion： In a selected Chinese population, AA genotype of Arg399Gln appears to contribute to a decreased risk of idiopathic azoospermia, while we have not any evidence of involvement of XRCC1 T-77C and Arg194Trp polymorphisms in idiopathic azoospermia. （Asian J Androl 2007 Nov; 9： 843-848）

关键词： DNA repair XRCC1 polymorphism male infertility idiopathic azoospermia

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