检索结果-南通市图书馆

An efficient and safe strategy for germ cell-specific automatic excision of foreign DNA in F1 hybrid transgenic silkworms

引用

Insect Science 2024年第1期31卷 28-46页

作者： Dingpei Long Rongpeng Liu Yang Huang Anyao fu Yuli Zhang Zhanzhang Hao qiang Li Hanfu Xu Zhonghuai Xiang Aichun Zhao State Key Laboratory of Resource Insects Institute of Sericulture and Systems BiologySouthwest UniversityChongqingChina Department of Biology Georgia State UniversityAtlantaGeorgiaUSA Guangxi Institute of Sericulture Science NanningGuangxiChina Department of Mathematics and Statistics Georgia State UniversityAtlantaGeorgiaUSA

The safety of transgenic technology is a major obstacle in the popularization and use of transgenic silkworms and their *** sericulture,only the first filial generation(F1)hybrid eggs produced by cross-breeding Japanese and Chinese original strains are usually used for the large-scale breeding of silkworms,but this may result in uncontrolled transgene dispersal during the popularization and application of the F1 hybrid transgenic *** address this issue,we developed a safe and efficient strategy using the GAL4/Upstream activating sequence(UAS)system,the FLP/flippase recognition target(FRT)system,and the gonad-specific expression gene promoters(RSHP1p and Nanosp)for the germ cell-specific automatic excision of foreign DNA in the F1 hybrid transgenic *** established 2 types of activator strains,R1p::GAL4-Gr and Nsp::GAL4-Gr,containing the testis-specific GAL4 gene expression cassettes driven by RSHP1p or Nanosp,respectively,and 1 type of effector strain,UAS::FLP-Rg,containing the UAS-linked FLP gene expression *** FLP recombinase-mediated sperm-specific complete excision of FRT-flanked target DNA in the F1 double-transgenic silkworms resulting from the hybridization of R1p::GAL4-Gr and UAS::FLP-Rg was 100%,whereas the complete excision efficiency resulting from the hybridization of Nsp::GAL4-Gr and UAS::FLP-Rg ranged from 13.73%to 80.3%.Additionally,we identified a gene,sw11114,that is expressed in both testis and ovary of Bombyx mori,and can be used to establish novel gonad-specific expression systems in transgenic *** strategy has the potential to fundamentally solve the safety issue in the production of F1 transgenic silkworm eggs and provides an important reference for the safety of transgenic technology in other insect species.

关键词： Bombyx mori FLP/FRT system GAL4/UAS system germ cell-specific excision safety of transgenic technology transgenic silkworm

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

New insights into the deposition of natural gas hydrate on pipeline surfaces:A molecular dynamics simulation study

引用

Petroleum Science 2024年第1期21卷 694-704页

作者： Jun Zhang Hai-qiang fu Mu-Zhi Guo Zhao Wang Li-Wen Li Qi Yin You-Guo Yan Wei Wei Wei-Feng Han Jie Zhong School of Materials Science and Engineering China University of Petroleum(East China)Qingdao 266580ShandongChina School of Petroleum Engineering China University of Petroleum(East China)Qingdao 266580ShandongChina Department of Alternative Energy PetroChina Research Institute of Petroleum Exploration&DevelopmentLangfang 065007HebeiChina

Natural gas hydrate(NGH)can cause pipeline blockages during the transportation of oil and gas under high pressures and low *** hydrate adhesion on pipelines is viewed as an efficient way to prevent NGH *** studies suggested the water film can greatly increase hydrate adhesion in gas-dominant ***,by performing the molecular dynamics simulations,we find in water-dominant system,the water film plays different roles in hydrate deposition on Fe and its corrosion ***,due to the strong affinity of water on Fe surface,the deposited hydrate cannot convert the adsorbed water into hydrate,thus,a water film *** water affinities decrease(Fe>Fe_(2)O_(3)>FeO>Fe_(3)O_(4)),adsorbed water would convert to amorphous hydrate on Fe_(2)O_(3)and form the ordered hydrate on FeO and Fe_(3)O_(4)after hydrate *** absorbed water film converts to amorphous or to hydrate,the adhesion strength of hydrate continuously increases(Fe

关键词： Deposition Natural gas hydrate Pipelines Water affinity Adhesion strength

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Oxidation behavior of 4774DD1 Ni-based single-crystal superalloy at 980℃ in air

引用

China Foundry 2024年第2期21卷 116-124页

作者： Yu Fang Ya-zhou Li qiang Yang Qun-gong He Xiu-fang Gong Qian Duan Hai-yang Song fu Wang Qiong-yuan Zhang Hong Zeng State Key Laboratory of Long-Life High Temperature Materials Dongfang Turbine Co.Ltd.Deyang 618000SichuanChina State Key Laboratory for Manufacturing System Engineering School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an 710049China

The oxidation behavior of a novel Ni-based single-crystal 4774DD1 superalloy for industrial gas turbine applications was investigated by the isothermal oxidation at 980℃ and discontinuous oxidation weight gain *** phase constitution and morphology of surface oxides and the characteristics of the crosssection oxide film were analyzed by XRD,SEM and *** show that the oxidation kinetics of the 4774DD1 superalloy follows the cubic law,indicating its weak oxidation resistance at this *** the oxidation time increases,the composition of the oxide film evolves as following:One layer consisting of a bottom Al_(2)O_(3)sublayer and an upper(Al_(2)O_(3)+NiO)mixture sublayer after oxidized for 25 ***,two layers composed of an outermost small NiO discontinuous grain layer and an internal layer for 75 *** internal layer is consisted of the bottom Al_(2)O_(3)sublayer,an intermediate narrow CrTaO_(4)sublayer,and an upper(Al_(2)O_(3)+NiO)mixture *** two layers comprising an outermost relative continuous NiO layer with large grain size and an internal layer as the oxidation time increases to 125 *** internal layer is composed of the upper(Al_(2)O_(3)+NiO)mixture sublayer,an intermediate continuous(CrTaO_(4)+NiWO_(4))mixture sublayer,and a bottom Al_(2)O_(3)***,three layers consisting of an outermost(NiAl2O_(4)+NiCr2O_(4))mixture layer,an intermediate(CrTaO_(4)+NiWO_(4))mixture layer,and a bottom Al_(2)O_(3)layer for 200 h.

关键词： nickel-base single crystal superalloy oxidation kinetics oxide film microstructure mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Portal vein embolization combined with ex vivo liver resection and autotransplantation: A novel treatment strategy for end-stage and metastatic hepatic alveolar echinococcosis

引用

Hepatobiliary & Pancreatic Diseases International 2024年第2期23卷 210-216页

作者： qiang Guo Mao-Lin Wang kai Zhong Jia-Long Li Tie-Min Jiang Hao Wen Tuerganaili Aji Ying-Mei Shao Department of Hepatobiliary and Echinococcosis Surgery Digestive and Vascular Surgery CenterFirst Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054China Clinical Medical Research Center of Echinococcosis and Hepatobiliary Disease of Xinjiang Uygur Autonomous Region Urumqi 830054China State Key Laboratory of Pathogenesis Prevention and Treatment of High Incidence Diseases in Central AsiaXinjiang Medical UniversityUrumqi 830054China

Alveolar echinococcosis(AE)is a lethal parasitic disease caused by Echinococcus multilocularis larvae,and more than 90%of pri-mary AE lesions occur in the *** of the affected individ-uals remain asymptomatic and the disease is often diagnosed at an advanced *** infection may spread to organs adjacent to the liver or distant locations,eventually causing end-stage multi-ple organ *** metastasis of AE is the most fatal with an inci-dence rate of 0.2%[1].

关键词： organs echinococcosis metastasis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Early systemic anticoagulation reduces hospital readmission in acute necrotizing pancreatitis patients:A retrospective cohort study

引用

Hepatobiliary & Pancreatic Diseases International 2024年第1期23卷 77-82页

作者： Wen-Jian Mao Jing Zhou Guo-fu Zhang Fa-Xi Chen Jing-Zhu Zhang Bai-qiang Li Lu Ke Wei-Qin Li Department of Critical Care Medicine Jinling HospitalNanjing Medical UniversityNanjing 210002China Department of Critical Care Medicine Jinling HospitalMedical School of Nanjing UniversityNanjing 210002China National Institute of Healthcare Data Science Nanjing UniversityNanjing 210010China

Background:Early systemic anticoagulation(SAC)is a common practice in acute necrotizing pancreatitis(ANP),and its impact on in-hospital clinical outcomes had been ***,whether it affects long-term outcomes is *** study aimed to evaluate the effect of SAC on 90-day readmission and other long-term outcomes in ANP ***:During January 2013 and December 2018,ANP patients admitted within 7 days from the onset of abdominal pain were *** primary outcome was 90-day readmission after *** proportional-hazards regression model and mediation analysis were used to define the relationship between early SAC and 90-day ***:A total of 241 ANP patients were enrolled,of whom 143 received early SAC during their hospitalization and 98 did *** who received early SAC experienced a lower incidence of splanchnic venous thrombosis(SVT)[risk ratio(RR)=0.40,95%CI:0.26-0.60,P<0.01]and lower 90-day readmission with an RR of 0.61(95%CI:0.41-0.91,P=0.02)than those who did *** the quality of life,patients who received early SAC had a significantly higher score in the subscale of vitality(P=0.03)while the other subscales were all comparable between the two *** Cox regression model showed that early SAC was an independent protective factor for 90-day readmission after adjusting for potential confounders with a hazard ratio of 0.57(95%CI:0.34-0.96,P=0.04).Mediation analysis showed that SVT mediated 37.0%of the early SAC-90-day readmission ***:The application of early SAC may reduce the risk of 90-day readmission in the survivors of ANP patients,and reduced SVT incidence might be the primary contributor.

关键词： Anticoagulation Splanchnic venous thrombosis Acute necrotizing pancreatitis Readmission Long-term outcomes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Triazine-based multicomponent metallacages with tunable structures for SO_(2) selective capture and conversion

Aggregate

引用

Aggregate 2024年第1期5卷 270-279页

作者： Ruoqian Zhang Dingyue Hu Yu fu Qian Feng Chaoqun Mu kai Gao Heping Ma Ming Liu Mingming Zhang State Key Laboratory for Mechanical Behavior of Materials Shaanxi International Research Center for Soft MatterSchool of Materials Science and EngineeringXi’an Jiaotong UniversityXi’anP.R.China Department of Chemistry Hangzhou Global Scientific and Technological Innovation Center(HIC)Zhejiang UniversityHangzhouP.R.China School of Chemical Engineering and Technology Xi’an Jiaotong UniversityXi’anP.R.China

The design of novel materials for sulfur dioxide(SO_(2))capture and conversion with considerable efficiency under mild conditions is of great significance for human health and environmental protection yet highly ***,we report a series of triazine-based multicomponent metallacages via coordination-driven self-assembly of 2,4,6-tri(4-pyridyl)-1,3,5-triazine,cis-Pt(PEt3)2(OTf)2 and different tetracarboxylic *** the increase of the length of the tetracarboxylates,the structures of the metallacages change from pyramids to extended *** to their N-rich structure,these metallacages are further used for selective SO_(2)capture,showing good adsorption capacity and remarkable SO_(2)/CO_(2)selectivity in ambient conditions,suggesting their potential applications toward real flue gas *** metallacages are further employed for the conversion of SO_(2)into value-added compounds,showing exceptional efficiency even dilute SO_(2)is used as the *** study represents a type of structure-tunable triazinebased metallacages for SO_(2)capture and conversion,which will pave the way on the applications of metal-organic complexes for gas adsorption.

关键词： multicomponent metallacages self-assembly SO_(2) adsorption SO_(2) conversion tunable structures

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Coordinated responses of leaf and nodule traits contribute to the accumulation of N in relay intercropped soybean

引用

Journal of Integrative Agriculture 2024年第6期23卷 1910-1928页

作者： Ping Chen Qing Du Benchuan Zheng Huan Yang Zhidan fu kai Luo Ping Lin Yilin Li Tian Pu Taiwen Yong Wenyu Yang College of Agronomy Sichuan Agricultural University/Sichuan Engineering Research Center for Crop Strip Intercropping System/Key Laboratory of Crop Ecophysiology and Farming System in SouthwestMinistry of Agriculture and Rural AffairsChengdu 611130China Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066China

Maize(Zea mays L.)-soybean(Glycine max ***.)relay intercropping provides a way to enhance land ***,the late-planted soybean suffers from shading by the *** maize harvest,how the recovery growth influences the leaf and nodule traits remains unclear.A three-year field experiment was conducted to evaluate the effects of genotypes,i.e.,supernodulating(nts1007),Nandou 12(ND12),and Guixia 3(GX3),and crop configurations,i.e.,the interspecific row spacing of 45(I45),60(I60),75 cm(I75),and sole soybean(SS),on soybean recovery growth and N *** results showed that intercropping reduced the soybean total leaf area(LA)by reducing both the leaf number(LN)and unit leaflet area(LUA),and it reduced the nodule dry weight(NW)by reducing both the nodule number(NN)and nodule diameter(ND)compared with the *** correlation and principal component analysis(PCA)indicated a co-variability of the leaf and nodule traits in response to the genotype and crop configuration *** the recovery growth stages,the compensatory growth promoted soybean growth to reduce the gaps of leaf and nodule traits between intercropping and *** relative growth rates of ureide(RGR_U)and nitrogen(RGR_N)accumulation were higher in intercropping than in *** achieved more significant sucrose and starch contents compared with ***12 and GX3 showed more robust compensatory growth than nts1007 in *** the recovery growth of relay intercropping soybean improved biomass and nitrogen accumulation,ND12 gained a more significant partial land equivalent ratio(pLER)than *** I60 treatment achieved more robust compensation effects on biomass and N accumulation than the other ***,I60 showed a higher nodule sucrose content and greater shoot ureide and N accumulation than ***,intercropping ND12 with maize using an interspecific row spacing of 60 cm was optimal for both yield advantage and N accumulation.

关键词： relay intercropping genotype crop configuration symbiotic nitrogen fixation soybean nodule

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Divergent driving mechanisms of community temporal stability in China's drylands

引用

Environmental Science and Ecotechnology 2024年第4期20卷 237-246页

作者： kai Wang Cong Wang Bojie fu Jianbei Huang Fangli Wei Xuejing Leng Xiaoming Feng Zongshan Li Wei Jiang State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing100085China College of Resources and Environment University of Chinese Academy of SciencesBeijing100049China Max Planck Institute for Biogeochemistry Hans-Kn€oll-Str.1007745JenaGermany Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing100101China Shaanxi Yan'an Forest Ecosystem National Observation and Research Station Beijing100085China National Observation and Research Station of Earth Critical Zone on the Loess Plateau in Shaanxi Xi'an710061China

Climate change and anthropogenic activities are reshaping dryland ecosystems globally at an unprecedented pace,jeopardizing their *** stability of these ecosystems is crucial for maintaining ecological balance and supporting local ***,the mechanisms governing their stability are poorly understood,largely due to the scarcity of comprehensive field *** we show the patterns of community temporal stability and its determinants across an aridity spectrum by integrating a transect survey across China's drylands with remote *** results revealed a U-shaped relationship between community temporal stability and aridity,with a pivotal shift occurring around an aridity level of *** less arid areas(aridity level below 0.88),enhanced precipitation and biodiversity were associated with increased community productivity and ***,in more arid zones(aridity level above 0.88),elevated soil organic carbon and biodiversity were linked to greater fluctuations in community productivity and reduced *** study identifies a critical aridity threshold that precipitates significant changes in community stability in China's drylands,underscoring the importance of distinct mechanisms driving ecosystem stability in varying aridity *** insights are pivotal for developing informed ecosystem management and policy strategies tailored to the unique challenges of dryland conservation.

关键词： Dryland ecosystems Community stability Ecosystem management Species richness Aridity threshold

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rapidly growing primordial black holes as seeds of the massive high-redshift JWST Galaxies

引用

Science China(Physics,Mechanics & Astronomy) 2024年第10期67卷 139-146页

作者： Guan-Wen Yuan Lei Lei Yuan-Zhu Wang Bo Wang Yi-Ying Wang Chao Chen Zhao-qiang Shen Yi-fu Cai Yi-Zhong Fan Key Laboratory of Dark Matter and Space Astronomy Purple Mountain ObservatoryChinese Academy of SciencesNanjing 210023China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Deep Space Exploration Laboratory/Department of Astronomy School of Physical SciencesUniversity of Science and Technology of ChinaHefei 230026China Jockey Club Institute for Advanced Study The Hong Kong University of Science and TechnologyHong KongChina

A group of massive galaxies at redshifts of z■7 have been recently detected by the James Webb Space Telescope(JWST),which were unexpected to form at such an early time within the standard Big Bang *** this work,we propose that this puzzle can be explained by the presence of some primordial black holes(PBHs)with a mass of~1000M_(⊙).These PBHs act as seeds for early galaxy formation with masses of~10^(8)-10^(10)M_(⊙)at high redshift,hence accounting for the JWST *** use a hierarchical Bayesian inference framework to constrain the PBH mass distribution models,and find that the Lognormal model with the M_(c)~750M_(⊙)is preferred over other *** rapidly growing BHs are expected to have strong radiation and may appear as high-redshift compact objects,similar to those recently discovered by *** we focused on PBHs in this work,the bound on the initial mass of the seed black holes remains robust even if they were formed through astrophysical channels.

关键词： primordial black hole dark matter halo black hole accretion primordial galaxies

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecule-based vertical transistor via intermolecular charge transport throughπ-πstacking

引用

Nano Research 2024年第5期17卷 4573-4581页

作者： Cheng Liu Cheng fu Lingyu Tang Jianghua Wu Zhangyan Mu Yamei Sun Yanghang Pan Bailin Tian kai Bao Jing Ma Qiyuan He Mengning Ding Key Laboratory of Mesoscopic Chemistry School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Jiangsu Key Laboratory of Advanced Organic Materials School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China National Laboratory of Solid State Microstructures Jiangsu Key Laboratory of Artificial Functional MaterialsCollege of Engineering and Applied SciencesCollaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China Department of Materials Science and Engineering City University of Hong KongHong Kong 999077China

Theπ-πstacking is a well-recognized intermolecular interaction that is responsible for the construction of electron hopping channels in numerous conducting frameworks/***,the exact role ofπ-to-πchannels within typical single crystalline organic semiconductors remains unclear as the orientations of these molecules are diverse,and their control usually requires additional side chain groups that misrepresent the intrinsic properties of the original semiconducting ***,the construction of conduction channels with intrinsicπ-πstacking in the molecule-based device is crucial for the utilization of their unique transport characteristics and understanding of the transport *** this end,we present a molecular intercalation strategy that integrates two-dimensional layered materials with functional organic semiconductor molecules for functional molecule-based *** organic semiconductor molecules can be effectively intercalated into the van der Waals gaps of semi-metallic TaS_(2) withπ-πstacking configuration and controlled intercalant *** results show that the vertical charge transport in the stacking direction shows a tunneling-dominated mechanism that strongly depends on the molecular ***,we demonstrated a new type of molecule-based vertical transistor in which TaS_(2) andπ-πstacked organic molecules function as the electrical contact and the active channel,***/off ratios as high as 447 are achieved under electrostatic modulation in ionic liquid,comparable to the current state-of-the-art molecular *** study provides an ideal platform for probing intrinsic charge transport acrossπ-πstacked conjugated molecules and also a feasible approach for the construction of high-performance molecule-based electronic devices.

关键词： π-πstacking electrochemical intercalation organic semiconductor electrical transport tunneling field effect transistor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：