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Engineered AAV13 variants with enhanced transduction and confined spread

Zoological Research 2024年第4期45卷 781-790页

作者： Neng-Song Luo Yu-Xiang Cai Zeng-Peng Han Xiao-kai Sui Wen-Jia Yuan Zi-Lian Zhang Hao-Dong Guo Jie Wang Kun-Zhang Lin Fu-qiang Xu Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhanHubei 430074China Shenzhen Key Laboratory of Viral Vectors for Biomedicine Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong 518055China Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhanHubei 430071China Key Laboratory of Quality Control Technology for Virus-Based Therapeutics Guangdong Provincial Medical Products AdministrationNMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal ProductsBrain Cognition and Brain Disease InstituteShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong 518055China University of Chinese Academy of Sciences Beijing 100049China Zhongnan Hospital Wuhan UniversityWuhanHubei 430072China Brain Case Biotechnology Co. Ltd.ShenzhenGuangdong 518107China College of Life Sciences Wuhan UniversityWuhanHubei 430072China Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China

Precise targeting of specific regions within the central nervous system(CNS)is crucial for both scientific research and gene therapy in the context of brain ***-associated virus 13(AAV13)is known for its restricted diffusion range within the CNS,making it an ideal choice for precise labeling and administration within small brain ***,AAV13 mediates relatively low expression of target ***,we introduced specifically engineered modifications to the AAV13 capsid protein to enhance its transduction *** first constructed AAV13-YF by mutating tyrosine to phenylalanine on the surface of the AAV13 *** then inserted the 7m8 peptide,known to enhance cell transduction,into positions 587/588 and 585/586 of the AAV13 capsid,resulting in two distinct variants named AAV13-587-7m8 and AAV13-585-7m8,*** found that AAV13-YF exhibited superior in vitro infectivity in HEK293T cells compared to AAV13,while AAV13-587-7m8 and AAV13-585-7m8 showed enhanced CNS infection capabilities in C57BL/6 mice,with AAV13-587-7m8 infection retaining a limited spread *** modified AAV13 variants hold promising potential for applications in gene therapy and neuroscience research.

关键词： Adeno-associated virus 13 AAV13-YF AAV13-587-7m8 AAV13-585-7m8 transduction efficiency

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Tourmaline geochemistry as genetic evolution and metallogenic potential indicators of pegmatites: a case study from the Tugeman Be-Li deposit， Middle Altun

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Journal of Earth Science 2024年

作者： Hang Li Xingwang Xu Tao Hong qiang Ke Yince Ma kai Kang Zhiquan Yang Changsheng Guo College of Architecture and Civil Engineering， Sanming University Key Laboratory of Mineral Resources， Institute of Geology and Geophysics， Chinese Academy of Sciences University of Chinese Academy of Sciences Innovation Academy for Earth Science， Chinese Academy of Sciences Guangdong Provincial Key Lab of Geodynamics and Geohazards， School of Earth Sciences and Engineering， Sun Yat-Sen University Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) No.3 Geological Party， Xinjiang Bureau of Geology and Mineral Exploration and Development

Tourmaline is a common borosilicate mineral in granite and pegmatite， and it is also an important carrier to record the physicochemical evolution information of magmas. However， the genetic relationship between pegmatite-type rare metal mineralization potential and tourmaline-bearing pegmatites is not clear， and the tourmaline geochemical indication of the mineralization type of rare metals needs to be supplemented. In the middle Altun region， extensive tourmaline-bearing pegmatite dykes are prevalent within and surrounding the Tugeman complex. In this paper， the petrology and geochemical characteristics of tourmaline within the pegmatite of the Tugeman Be-Li deposit have been comprehensively analyzed. The research delves into the genetic evolution and metallogenic potential of the pegmatite， emphasizing the indicative role of tourmaline geochemistry in discerning mineralization types in the Tugeman Be-Li deposit. Major and trace element analysis of tourmaline reveals varying contents of FeO （9.37 wt.%–17.10 wt.%）， Al2O3（28.64 wt.%–35.22% wt.%）， and MgO （0.13 wt.%–2.47 wt.%） in the Tugeman Be–Li pegmatite. Li， Zn， and Sn contents range from 204 ppm to 1 590 ppm， 1 143 ppm to 4 089 ppm， and 74 ppm to 396 ppm， respectively. The tourmaline analyzed in this study is magmatic origin and belongs to schorl series， exhibits substitution relationships， including (Na， Fe2+)(X□， Al)-1， (Fe2+， Fe3+)（Mg， Al）-1， (Al，X□)(R2+， Na)-1，（Na， Mg）(X□， Al)-1andX□NaR2+-1. The trace element data of tourmaline show that lithium has four enrichment stages in different mineral crystallization phases. Notably， the tendency of tourmaline in the Tugeman Be-Li pegmatite to evolve towards elbaite implies a magmatic evolution sequence from low to high， transitioning through microcline-albite pegmatite， muscovite-microcline-albite pegmatite， albite pegmatite， and microcline pegmatite. Furthermore， we also propose that the Sc content in schorl can serve as an indicator of pegmatite mineralization typ

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Rapid development of double-hit mRNA antibody cocktail against orthopoxviruses

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1768-1776页

作者： Hang Chi Suo-Qun Zhao Ru-Yi Chen Xing-Xing Suo Rong-Rong Zhang Wen-Hui Yang Dong-Sheng Zhou Min Fang Bo Ying Yong-qiang deng Cheng-Feng Qin State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyAMMS100071BeijingChina College of Life Sciences Inner Mongolia Agricultural UniversityHohhot010018Inner MongoliaChina School of Life Sciences Henan UniversityKaifeng475004HenanChina Suzhou Abogen Biosciences Co. LtdSuzhou215123JiangsuChina Research Unit of Discovery and Tracing of Natural Focus Diseases Chinese Academy of Medical Sciences100071BeijingChina

The Orthopoxvirus genus,especially variola virus(VARV),monkeypox virus(MPXV),remains a significant public health threat *** development of therapeutic antibodies against orthopoxviruses is largely hampered by the high cost of antibody engineering and manufacturing ***-encoded antibodies have emerged as a powerful and universal platform for rapid antibody ***,by using the established lipid nanoparticle(LNP)-encapsulated mRNA platform,we constructed four mRNA combinations that encode monoclonal antibodies with broad neutralization activities against *** vivo characterization demonstrated that a single intravenous injection of each LNP-encapsulated mRNA antibody in mice resulted in the rapid production of neutralizing *** importantly,mRNA antibody treatments showed significant protection from weight loss and mortality in the vaccinia virus(VACV)lethal challenge mouse model,and a unique mRNA antibody cocktail,Mix2a,exhibited superior in vivo protection by targeting both intracellular mature virus(IMV)-form and extracellular enveloped virus(EEV)-form *** summary,our results demonstrate the proof-of-concept production of orthopoxvirus antibodies via the LNP-mRNA platform,highlighting the great potential of tailored mRNA antibody combinations as a universal strategy to combat orthopoxvirus as well as otheremerging viruses.

关键词： proof universal neutral

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Application of UiO-66 and its composites for remediation and resource recovery of typical environmental contaminants:a review

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Rare Metals 2024年第6期43卷 2498-2526页

作者： Hui-Min Zhang Yun-Long Wang Xin-Feng Zhu Zhen-Zhen Huang Dan-Dan Pang kai Wang Chao-Hai Wang Zhong-Xian Song Shi-qiang Yin Lin-Lin Chang Jin-Hui Zhang School Infirmary Henan University of Urban ConstructionPingdingshan 467036China Faculty of Environmental and Municipal Engineering Henan Key Laboratory of Water Pollution Control and Rehabilitation TechnologyKey Laboratory of Carbon Emission Reduction and Combined Air Pollution Control TechnologyHenan University of Urban ConstructionPingdingshan 467036China School of Resources&Environment Collaborative Innovation Center of Coalbed Methane and Shale Gas for Central Plains Economic RegionHenan Polytechnic UniversityJiaozuo 454003China The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters Ministry of EducationGuangzhou Higher Education Mega CentreSouth China University of TechnologyGuangzhou 510006China

UiO-66 series metal-organic framework materials(MOFs)are typical porous materials assembled by Zr^(4+) with a large mass-to-nucleus ratio and terephthalic acid ligands,which form tetrahedral and octahedral cages arranged in a periodic triangular window *** to the strong interaction between Zr and O,UiO-66 series MOFs exhibit high thermal stability,structural stability,and chemical *** article mainly reviews the applications of UiO-66 and its composites in adsorption,photocatalysis,and resource utilization,while exploring the harm of pollutants to human health and the *** the first part,the differences in adsorption and removal mechanisms of liquid organic pollutants,heavy metals,and volatile organic compounds(VOCs)are *** results show that organic pollutants are mainly removed by physical adsorption,electrostatic interactions,hydrogen bonding,andπ-πinteractions,while heavy metals are mainly removed by chemical adsorption,electrostatic interactions,reduction,and *** are mainly removed by the pore volume and pore size structure of the *** catalysis can achieve non-toxic treatment of pollutants,and this study mainly focuses on UiO-based composite materials constructed by strategies such as semiconductor composites,ion doping,and metal/dye *** the second part,the synergistic effect between the components of UiO-based composite materials promotes the oriented and rapid separation and transfer of earriers at the material interface,thereby promoting the generation of active species such as h^(+),·O_(2)^(-) and·OH,and achieving rapid degradation of pollutants and detoxification of heavy *** the third part,heterojunctions can realize the resource utilization of pollutants in water and air,producing energy-type substances such as hydrogen and methanol while solving environmental *** addition,this article also summarizes the harm of common typical pollutants to the environment

关键词： UiO Adsorption Photocatalysis Environmental pollution Resource recovery

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Achieving high carrier mobility and low lattice thermal conductivity in GeTe-based alloys by cationic/anionic co-doping

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Rare Metals 2024年第6期43卷 2784-2795页

作者： Xiao-qiang Wang Xiao-Quan Hu Jun-Yan Lin Chu-Bin Li Xiao-Tong Yu Qi-Yong Chen Li-Li Xi Qi-Shuo Yang Han Li Ji-Ye Zhang Shuan-Kui Li kai Guo School of Physics and Materials Science Guangzhou UniversityGuangzhou 510006China Research Center for Advanced Information Materials(CAIM) Huangpu Research&Graduate School of Guangzhou UniversitySino-Singapore Guangzhou Knowledge CityGuangzhou510555China School of Materials Science and Engineering Shanghai UniversityShanghai 200444China Materials Genome Institute Shanghai UniversityShanghai 200444China School of Mechanical and Mining Engineering University of QueenslandBrisbaneQLD 4072Australia Key Laboratory of Si-Based Information Materials&Devices and Integrated Circuits Design Department of Education of Guangdong ProvinceGuangzhou 510006China

TheⅣ-Ⅵcompound GeTe is considered as a promising alternative to the toxic PbTe for high-efficiency mid-temperature thermoelectric ***,pristine GeTe suffers from a high concentration of Ge vacancies,resulting in an excessively high hole concentration(>1×10^(21)cm^(-3)),which greatly limits its thermoelectric *** address this issue,CuBiTe_(2)alloying is introduced to increase the formation energy of Ge vacancies in GeTe,thereby inhibiting the high carrier *** carrier scattering caused by the electronegativity difference between different elements is suppressed due to the similar electronegativity of Cu and Ge atoms.A relatively high hole mobility is obtained,which ultimately leads to a high power ***,by introducing Se as an alloying element at the anionic site in GeTe,dense point defects with mass/strainfield fluctuations are *** contributes to the strengthening of phonon scattering,thereby reducing the lattice thermal conductivity from 1.44 W·m^(-1)·K^(-1)for pristine GeTe to 0.28 W·m^(-1)·K^(-1)for Ge_(0.95)Cu_(0.05)Bi_(0.05)Te_(0.9)Se_(0.15)compound at 623 K.

关键词： GeTe Carrier mobility CuBiTe_(2)alloying Lattice thermal conductivity Thermoelectric properties

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Natural evidence of coronaviral 2′-O-methyltransferase activity affecting viral pathogenesis via improved substrate RNA binding

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2819-2834页

作者： Jikai deng Shimin Yang Yingjian Li Xue Tan Jiejie Liu Yanying Yu qiang Ding Chengpeng Fan Hongyun Wang Xianyin Chen Qianyun Liu Xiao Guo Feiyu Gong Li Zhou Yu Chen State Key Laboratory of Virology RNA InstituteCollege of Life Sciences and Frontier Science Center for Immunology and MetabolismWuhan UniversityWuhanChina School of Medicine Tsinghua UniversityBeijingChina School of Basic Medical Sciences Wuhan UniversityWuhanChina Animal Bio-Safety Level Ⅲ Laboratory/Institute for Vaccine Research Wuhan University School of MedicineWuhanChina

Previous studies through targeted mutagenesis of K-D-K-E motif have demonstrated that 2′-O-MTase activity is essential for efficient viral replication and immune ***,the K-D-K-E catalytic motif of 2′-O-MTase is highly conserved across numerous viruses,including flaviviruses,vaccinia viruses,coronaviruses,and extends even to ***,we observed a stronger 2′-O-MTase activity in SARS-CoV-2 compared to SARS-CoV,despite the presence of a consistently active catalytic *** further identified critical residues(Leu-36,Asn-138 and Ile-153)which served as determinants of discrepancy in 2′-O-MTase activity between SARS-CoV-2 and *** residues significantly enhanced the RNA binding affinity of 2′-O-MTase and boosted its versatility toward RNA *** interest,a triple substitution(Leu^(36)→Ile^(36),Asn^(138)→His^(138),Ile^(153)→Leu^(153),from SARS-CoV-2 to SARS-CoV)within nsp16 resulted in a proportional reduction in viral 2′-O-methylation and impaired viral ***,it led to a significant upregulation of type I interferon(IFN-I)and proinflammatory cytokines both in vitro and vivo,relying on the cooperative sensing of melanoma differentiation-associated protein 5(MDA5)and laboratory of genetics and physiology 2(LGP2).In conclusion,our findings demonstrated that alterations in residues other than K-D-K-E of 2′-O-MTase may affect viral replication and subsequently influence *** changes in nsp16 residues is crucial as it may aid in identifying and assessing future alteration in viral pathogenicity resulting from natural mutations occurring in nsp16.

关键词： pathogenesis impaired alterations

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A complete assembly of the sorghum BTx623 reference genome

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Plant Communications 2024年第6期5卷 13-16页

作者： Yuan deng Peng Zhou Fei Li Jing Wang Kun Xie Hongkai Liang Chunchao Wang Bin Liu Zhenxing Zhu Wenbin Zhou Baoqing Dun Xiaochun Lu Xianmin Diao qiang He State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Crop Molecular Improvement Lab Liaoning Academy of Agricultural SciencesShenyangLiaoning 110161China Zhongyuan Research Center Chinese Academy of Agricultural SciencesXinxiangHenan 453599China

Dear Editor,Sorghum(Sorghum bicolor),recognized as the world’s fifth most significant cereal crop,plays an essential role as a model for genomic comparison and functional genomics of C4 *** was domesticated in Ethiop... 详细信息

关键词： ). Sorghum environments

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Thermodynamics of charged black holes in Maxwell-dilaton-massive gravity

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Chinese Physics C 2024年第7期48卷 232-243页

作者：岳瑞宏钱凯强刘博邹德成 Center for Gravitation and Cosmology College of Physical Science and TechnologyYangzhou UniversityYangzhou 225009China School of Physics Northwest UniversityXi'an 710127China School of Physics and Information Science Shaanxi University of Science and TechnologyXi'an 710021China College of Physics and Communication Electronics Jiangxi Normal UniversityNanchang 330022China

Considering the nonminimal coupling of the dilaton field to the massive graviton field in Maxwelldilaton-massive gravity,we obtain a class of analytical solutions of charged black holes,which are neither asymptotically flat nor(A)*** calculated thermodynamic quantities,such as mass,temperature,and entropy,verify the validity of the first law of black hole ***,we further investigate the critical behaviors of these black holes in the grand canonical and canonical ensembles and find a novel critical phenomenon never before observed,known as the"reverse"reentrant phase transition with a tricritical *** implies that the system undergoes a novel"SBH-LBH-SBH"phase transition process and is the reverse of the"LBH-SBH-LBH"process observed in reentrant phase transitions.

关键词： Maxwell-dilaton-massive gravity charged black hole reverse reentrant phase transition VdW-like phase transitions

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Inverse-designed Jones matrix metasurfaces for high-performance meta-polarizers

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Chinese Optics Letters 2024年第2期22卷 192-199页

作者：王志强李枫竣邓倩媚万舟李向平邓子岚 Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications Institute of Photonics TechnologyJinan UniversityGuangzhou 510632China

Polarizers have always been an important optical component for optical engineering and have played an indispensable part of polarization imaging *** polarizers provide an excellent platform to achieve miniaturization,high resolution,and low cost of polarization imaging ***,we proposed freeform metasurface polarizers derived by adjoint-based inverse design of a full-Jones matrix with gradient-descent *** designed multiple freeform polarizers with different filtered states of polarization(SOPs),including circular polarizers,elliptical polarizers,and linear polarizers that could cover the full Poincaré*** that near-unitary polarization dichroism and the ultrahigh polarization extinction ratio(ER)reaching 50 d B were achieved for optimized circular *** multiple freeform polarizers with filtered polarization state locating at four vertices of an inscribed regular tetrahedron of the Poincarésphere are designed to form a full-Stokes parameters micropolarizer *** work provides a novel approach,we believe,for the design of meta-polarizers that may have potential applications in polarization imaging,polarization detection,and communication.

关键词： metasurface inverse design Jones matrix polarization imaging

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Strong light–matter interactions based on excitons and the abnormal all-dielectric anapole mode with both large field enhancement and low loss

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Photonics Research 2024年第4期12卷 854-864页

作者： YAN-HUI deng YU-WEI LU HOU-JIAO ZHANG ZHONG-HONG SHI ZHANG-kai ZHOU XUE-HUA WANG State Key Laboratory of Optoelectronic Materials and Technologies School of PhysicsSun Yat-sen UniversityGuangzhou 510275China Quantum Science Center of Guangdong–Hong Kong–Macao Greater Bay Area(Guangdong) Shenzhen 518045China

The room temperature strong coupling between the photonic modes of micro/nanocavities and quantum emitters(QEs) can bring about promising advantages for fundamental and applied *** the electric fields(EFs) by using plasmonic modes and reducing their losses by applying dielectric nanocavities are widely employed approaches to achieve room temperature strong ***,ideal photonic modes with both large EFs and low loss have been ***,we propose the abnormal anapole mode (AAM),showing both a strong EF enhancement of~70-fold (comparable to plasmonic modes) and a low loss of 34 meV,which is much smaller than previous records of isolated all-dielectric *** realizing strong coupling,we further show that by replacing the normal anapole mode with the AAM,the lasing threshold of the AAM-coupled QEs can be reduced by one order of magnitude,implying a vital step toward on-chip integration of nanophotonic devices.

关键词： dielectric mode coupling

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