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Brassica napus L. Homeodomain Leucine-Zipper Gene BnHB6 Responds to Abiotic and Biotic Stresses

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Journal of Integrative Plant Biology 2005年第10期47卷 1236-1248页

作者： Shun-Wu YU Li-Da ZHANG kai-jing zuo Dong-Qin TANG Xiao-Fen SUN Ke-Xuan TANG Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 China State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China

A homeodomain leucine-zipper （HD-Zip） gene BnHB6 （GenBank accession No. AY336103） was isolated from oilseed rape （Brassica napus L.） following drought treatment through rapid amplification of cDNA ends （RACE）. The full-length cDNA of BnHB6 was 1 611 bp and contained a 936-bp open reading frame encoding 311 amino acids. Sequence analysis indicated that BnHB6 belonged to the HD-Zip I subfamily. High-stringency Southern boltting analysis showed that BnHB6 appeared in rape as a single copy but had homologous genes. Semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） analysis revealed that BnHB6 was expressed in several tissues tested under control conditions, but that expression was significantly upregulated in shoots by mannitol, NaCI, cold treatment, anaerobic culture, wounding, H2O2, abscisic acid （ABA）, and salicylic acid （SA） treatments, but not by ultraviolet treatment. Further RT- PCR analysis revealed that BnHB6 was a late-responsive gene, the expression of which was not activated by NaCI, cold treatment, H2O2, ABA, and SA at an early time point （20 min） of treatment in the shoot. However, after a certain period of treatment, the induced expression culminated and then declined until the next peak occurred. Tissue-specific analysis revealed that BnHB6 was expressed at certain levels in the roots, shoots, and flowers, and the roots were found to respond to the osmotic stimuli more rapidly than shoots to increase the expression of BnHB6. The present study implies that BnHB6 plays a positive role as a regulator of biotic and abiotic stresses on growth during seedling establishment.

关键词： Brassica napus homeodomain leucine-zipper gene water stress.

A New Farnesyl Diphosphate Synthase Gene from Taxus media Rehder:Cloning,Characterization and Functional Complementation

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Journal of Integrative Plant Biology 2006年第6期48卷 692-699页

作者： Zhi-Hua Liao Min Chen Yi-Fu Gong Zhu-Gang Li kai-jing zuo Peng Wang Feng Tan Xiao-Fen Sun Ke-Xuan Tang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China Laboratory of Natural Products and Metabofic Engineering Institute of Biotechnology Chongqing Sweetpotato Research Center School of Life Sciences Southwest University Chongqing 400715 China Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 China Faculty of Life Sciences and Biotechnology Ningbo University Ningbo 315211 China

Farnesyl dlphosphate synthase （FPS; EC 2.***.1.10） catalyzes the production of 15-carbon farnesyl dlphosphate which Is a branch-point Intermediate for many terpenoids. This reaction Is considered to be a ratelimiting step In terpenold biosynthesis. Here we report for the first time the cloning of a new full-length cDNA encoding farnesyl dlphosphate synthase from a gymnosperm plant species, Taxus media Rehder, designated as TmFPS1. The full-length cDNA of TmFPS1 （GenBank accession number： AY461811） was 1 464 bp with a 1 056-bp open reading frame encoding a 351-amino acid polypeptlde with a calculated molecular weight of 40.3 kDa and a theoretical pl of 5.07. Biolnformatlc analysis revealed that TmFPS1 contained all five conserved domains of prenyltransferases, and showed homology to other FPSs of plant origin. Phylogenetlc analysis showed that farnesyl dlphosphate synthases can be divided Into two groups： one of prokaryotic origin and the other of eukaryotic origin. TmFPS1 was grouped with FPSs of plant origin. Homologybased structural modeling showed that TmFPS1 had the typical spatial structure of FPS, whose most prominent structural feature Is the arrangement of 13 core helices around a large central cavity In which the catalytic reaction takes place. Our blolnformatic analysis strongly suggests that TmFPS1 is a functional gene. Southern blot analysis revealed that TmFPS1 belongs to a small FPSgene family in T. media. Northern blot analysis indicated that TmFPS1 is expressed in all tested tissues, Including the needles, stems and roots of T. media. Subsequently, functional complementatlon with TmFPS1 in a FPS-deflclent mutant yeast demonstrated that TmFPS1 did encode farnesyl dlphosphate synthase, which rescued the yeast mutant. This study will be helpful In future Investigations aiming at understanding the detailed role of FPS In terpenold biosynthesis flux control at the molecular genetic level.

关键词： farnesyl diphosphate synthase rapid amplification of cDNA ends Taxus media yeast complementation

Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

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Neural Regeneration Research 2025年第3期20卷 821-835页

作者： jing Wang Bing Zhang Lanfang Li Xiaomei Tang Jinyu Zeng Yige Song Chao Xu kai Zhao Guoqiang Liu Youming Lu Xinyan Li kai Shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive ***,the mechanism underlying this neurogenesis impairment remains *** this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain *** results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and *** with repetitive traumatic brain injuryalso showed deficits in spatial memory ***,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was *** inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological *** findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury

AAV mediated carboxyl terminus of Hsp70 interacting protein overexpression mitigates the cognitive and pathological phenotypes of APP/PS1 mice

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Neural Regeneration Research 2025年第1期20卷 253-264页

作者： Zhengwei Hu jing Yang Shuo Zhang Mengjie Li Chunyan zuo Chengyuan Mao Zhongxian Zhang Mibo Tang Changhe Shi Yuming Xu Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Academy of Medical Sciences of Zhengzhou University ZhengzhouHenan ProvinceChina NHC Key Laboratory of Prevention and treatment of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Henan Key Laboratory of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Institute of Neuroscience Zhengzhou UniversityZhengzhouHenan ProvinceChina Sino-British Research Centre for Molecular Oncology National Centre for International Research in Cell and Gene TherapySchool of Basic Medical SciencesAcademy of Medical SciencesZhengzhou UniversityZhengzhouHenan ProvinceChina Department of Gerontology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina

The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control *** this study,we aimed to investigate the neuroprotective effect of overexpressed CHIP on Alzheimer’s *** used an adeno-associated virus vector that can cross the blood-brain barrier to mediate CHIP overexpression in APP/PS1 mouse *** overexpression significantly ameliorated the performance of APP/PS1 mice in the Morris water maze and nest building tests,reduced amyloid-βplaques,and decreased the expression of both amyloid-βand phosphorylated *** also alleviated the concentration of microglia and astrocytes around *** APP/PS1 mice of a younger age,CHIP overexpression promoted an increase in ADAM10 expression and inhibitedβ-site APP cleaving enzyme 1,insulin degrading enzyme,and neprilysin *** of HSP70 and HSP40,which have functional relevance to CHIP,were also *** nuclei transcriptome sequencing in the hippocampus of CHIP overexpressed mice showed that the lysosomal pathway and oligodendrocyte-related biological processes were up-regulated,which may also reflect a potential mechanism for the neuroprotective effect of *** research shows that CHIP effectively reduces the behavior and pathological manifestations of APP/PS1 ***,overexpression of CHIP could be a beneficial approach for the treatment of Alzheimer’s disease.

关键词： adeno-associated virus Alzheimer’s disease APP/PS1 mice carboxyl terminus of Hsp70 interacting protein gene therapy

An Efficient Boron Source Activation Strategy for the Low‑Temperature Synthesis of Boron Nitride Nanotubes

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Nano-Micro Letters 2025年第1期17卷 548-558页

作者： Ying Wang kai Zhang Liping Ding Liyun Wu Songfeng E Qian He Nanyang Wang Hui zuo Zhengyang Zhou Feng Ding Yue Hu Jin Zhang Yagang Yao National Laboratory of Solid State Microstructures College of Engineering and Applied SciencesJiangsu Key Laboratory of Artificial Functional Materialsand Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093People’s Republic of China School of Electronic Information and Artificial Intelligence Shaanxi University of Science&TechnologyXian 710000People’s Republic of China College of Bioresources Chemical and Materials Engineering Shaanxi University of Science&TechnologyXian 710000People’s Republic of China Key Laboratory of Carbon Materials of Zhejiang Province College of Chemistry and Materials EngineeringWenzhou UniversityWenzhou 325000People’s Republic of China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhen 518000People’s Republic of China College of Chemical and Molecular Engineering Peking UniversityBeijing 100871People’s Republic of China

Lowering the synthesis temperature of boron nitride nanotubes(BNNTs)is crucial for their *** primary reason for adopting a high temperature is to enable the effective activation of highmelting-point solid *** this study,we developed a novel approach for efficiently activating boron by introducing alkali metal compounds into the conventional MgO–B *** approach can be adopted to form various low-melting-point AM–Mg–B–O growth *** growth systems have improved catalytic capability and reactivity even under low-temperature conditions,facilitating the synthesis of BNNTs at temperatures as low as 850℃.In addition,molecular dynamics simulations based on density functional theory theoretically demonstrate that the systems maintain a liquid state at low temperatures and interact with N atoms to form BN *** findings offer novel insights into the design of boron activation and are expected to facilitate research on the low-temperature synthesis of BNNTs.

关键词： Boron nitride nanotubes Low-temperature Boron activation Density functional theory

西南地区河成湖与火山湖土地利用对水质的多时空尺度影响差异

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湖泊科学 2025年第1期37卷 132-148,I0011-I0014页

作者：刘捷刘睿左书林张静重庆师范大学地理与旅游学院重庆401331 重庆师范大学 GIS应用研究重庆市重点实验室重庆401331

为探究不同时空尺度下不同湖泊类型土地利用对水质的影响差异,以四川省自贡市富顺西湖(河成湖)和云南省腾冲市青海湖(火山湖)为研究对象,提取6个空间尺度(50、60、70、80、90、100 m湖岸带缓冲区)的景观格局指数,结合雨季(2022年7月)和... 详细信息

为探究不同时空尺度下不同湖泊类型土地利用对水质的影响差异,以四川省自贡市富顺西湖(河成湖)和云南省腾冲市青海湖(火山湖)为研究对象,提取6个空间尺度(50、60、70、80、90、100 m湖岸带缓冲区)的景观格局指数,结合雨季(2022年7月)和旱季(2023年4月)湖泊水质参数,采用冗余分析法定量探究不同时空尺度下土地利用对两类湖泊水质影响的差异性。结果表明:(1)富顺西湖与腾冲青海湖水质参数季节性差异显著,雨季水质优于旱季,两个湖泊水环境质量存在显著差异,腾冲青海湖水环境质量优于富顺西湖,腾冲青海湖符合地表水环境Ⅱ类、Ⅲ类水质标准,富顺西湖达到Ⅳ类、Ⅴ类水质标准。(2)富顺西湖在旱季和50~60 m湖岸带缓冲区尺度下土地利用参数对水质变量的解释率最大,腾冲青海湖在雨季和60 m湖岸带缓冲区尺度下土地利用参数对水质变量的解释率最大。(3)土地利用指数对两类湖泊水质的影响各不相同。富顺西湖流域内斑块密度、景观形状指数和聚合度指数与多数水质指标呈正相关,“源”景观聚集程度和破碎化程度高会加剧对水质的威胁。腾冲青海湖流域内林地面积占比和聚合度指数对水质具有正效应,能够改善水环境质量;斑块指数和景观形状指数值的增加会加剧斑块破碎化,导致水质恶化。本研究基于不同时空尺度对土地利用与两类湖泊水质关系的差异性进行深入探讨,指导不同类型湖泊的区域规划与管理,为湖泊环境质量评价及保护和制定针对性的湖泊缓冲带设置、生态修复等策略提供科学依据,进而促进湖泊水质安全和水资源可持续利用。

关键词：土地利用水质多尺度效应冗余分析西南地区湖泊

Construction of Mutant Populations by T-DNA Insertion for Functional Genomic Study in Cotton

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棉花学报 2002年第S1期14卷 48-48页

作者： Jia-he WU, kai-jing zuo, Yi-chun NIE, Xian-long ZHANG(National Key Laboratory for Genetic Improvements of Crops, Huazhong Agricultural University, Wuhan 430070, China) National Key Laboratory for Genetic Improvements of Crops Huazhong Agricultural University Wuhan 430070 China

The use of transfer DNA(T-DNA)as amutagen has been developed for tagging genes inmany crops,and results showed that T-DNAinsertion is a random event,and that theinserted genes are stable through *** sequencing PCR-amplifiedfragments adjacent to the inserted elements,wecan construct the T-DNA flanking database,which would be useful for cloning the genestagged by T-DNA.

关键词： Cotton DNA Mutant cloning Genomic sequencing inserted crops cotton mutant

Molecular Linkage Map Construction and QTL Mapping for Partial Agronomic Traits in Upland Cotton

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棉花学报 2002年第S1期14卷 22-22页

作者： Xian-long ZHANG, kai-jing zuo,Ji-zhong SUN, Yi-chun NIE(National Key Laboratory for Genetic Improvements of Crops, Huazhong Agricultural University, Wuhan 430070,China) National Key Laboratory for Genetic Improvements of Crops Huazhong Agricultural University Wuhan 430070China

Molecular map construction is very important togene marking,map-based cloning and marker-assisted *** advance was made inthis area in the US(Shappley et al,1996;1998;Reinisch et al,1994),but little work was donein China.A preliminary molecular map withRFLP,RAPD and SSR was constructed andQTL maping was dong for some agronomic traitsby our research group.

关键词： Cotton Linkage Traits agronomic breeding cloning marking preliminary cotton marker

基于LS-DNYA的圆棒数控折弯有限元分析及工艺参数研究

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太原学院学报（自然科学版） 2025年第1期43卷 71-77页

作者：左黎明雍凯左黎伟芜湖职业技术学院智能制造学院安徽芜湖241006 安徽瑞泰汽车零部件有限责任公司安徽池州242803

回弹、开裂、起皱等缺陷是折弯成型过程中的重要缺陷,严重影响成型质量和尺寸精度,因此利用ANSYS LS-DNYA模块针对数控折弯圆棒的过程进行非线性有限元分析,实现对圆棒的折弯效果有效模拟和准确预测。针对圆棒折弯速度设置4组分析实验组... 详细信息

回弹、开裂、起皱等缺陷是折弯成型过程中的重要缺陷,严重影响成型质量和尺寸精度,因此利用ANSYS LS-DNYA模块针对数控折弯圆棒的过程进行非线性有限元分析,实现对圆棒的折弯效果有效模拟和准确预测。针对圆棒折弯速度设置4组分析实验组,并进行弯曲分析,重点研究最大应力的位置和脱约束的指定节点自由回弹量。针对回弹量仿真数据,设置回弹补偿5组实物折弯试验,经过现场折弯试验证明,有限元分析数据准确可靠,研究结果为圆棒数控折弯的工艺优化和折弯质量提升提供了参考。

关键词：应力集中回弹有限元分析折弯速度