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Adsorption,in vitro digestion and human gut microbiota regulation characteristics of three Poria cocos polysaccharides

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Food Science and Human Wellness 2024年第3期13卷 1685-1697页

作者： fangming zhang Hui Zheng Tao Zheng Pan Xu Yao Xu Yuxin Cao fan Jia Yiqiong Zeng Yubing fan kai He Xinwen Dai Fengfei Hou Yong Yang College of Pharmacy Hunan University of Chinese MedicineChangsha 410208China School of Pharmaceutical Science Hunan University of MedicineHuaihua 418000China Hunan Fuling Engineering Technology Research Center Huaihua 418100China

Poria cocos(PC)is a famous traditional Chinese medicine(TCM)and a widely used healthcare ingredient,which has antiobesity,enhancing immunity and improving sleep ***,only water-soluble poria polysaccharide(WSP)is extracted and applied for clinical application,while insoluble polysaccharide(alkali-soluble poria polysaccharide,ASP)is discarded as herb ***,the whole PC has also been historically utilized as functional herbal *** the beneficial role of dietary fiber and the traditional use of PC,ASP may also contribute substantially to the therapy function of *** to WSP,little attention has been paid to ASP and ASP modified product carboxymethyl poria polysaccharide(CMP)which has been used as an antitumor adjuvant *** this study,the oil,cholesterol,metal ions and polyphenols adsorption ability,in vitro simulated digestive and the gut microbiota fermentation characteristics of WSP,ASP and CMP were studied to evaluate the functional values of three *** polysaccharides(PCPs).The results showed that all three PCPs had good adsorption capacity on cholesterol,polyphenols and metal ions(Cd^(2+)/Zn^(2+)/Mg^(2+)),among which ASP showed the highest capacity than WSP and *** adsorption capacity of all three PCPs on heavy metal ions(Cd^(2+)/Zn^(2+))was stronger than that of non-heavy metal ions(Mg^(2+));The in vitro digestibility of all three PCPs was very low,but WSP was slightly higher than ASP and CMP;Moreover,the indigestible residue of all three PCPs could improve the richness and diversity of gut microbiota,among which ASP had the greatest *** general,ASP and CMP could significantly promote the proliferation of some probiotics and inhibit the growth of some harmful *** gut microbiota diversity of CMP was reduced,but the richness of probiotics,especially Parabacteroides distasonis was significantly enhanced compared with the ASP group,and the growth of harmful bacteria Klebsiella pneumoniae was inh

关键词： Poria cocos polysaccharides Alkali-soluble poria polysaccharide Carboxymethyl poria polysaccharide Adsorption characteristics In vitro simulated digestion In vitro simulated gut microbiota fermentation

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Chinese Journal of Electronics 2024年第3期33卷 672-682页

作者： Yi zhang kai zhang Ting CUI PLA SSF Information Engineering University

Related-key model is a favourable approach to improve attacks on block ciphers with a simple key ***,to the best of our knowledge,there are a few results in which zero-correlation linear attacks take advantage of the related-key *** ascribe this phenomenon to the lack of consideration of the key input in zerocorrelation linear *** on the linear key schedule of a block cipher,we generalize the zero-correlation linear attack by using a related-key ***,we propose the creation of generalized linear hulls(GLHs)when the key input is involved;moreover,we indicate the links between GLHs and conventional linear hulls(CLHs).Then,we prove that the existence of zero-correlation GLHs is completely determined by the corresponding CLHs and the linear key *** addition,we introduce a method to construct zero-correlation GLHs by CLHs and transform them into an integral *** correctness is verified by applying it to SIMON16/16,a SIMON-like toy *** on our method,we find 12/13/14/15/15/17/20/22-round related-key zero-correlation linear distinguishers of SIMON32/64,SIMON48/72,SIMON48/96,SIMON64/96,SIMON64/128,SIMON96/144,SIMON128/192 and SIMON 128/256,*** far as we know,these distinguishers are one,two,or three rounds longer than current best zero-correlation linear distinguishers of SIMON.

关键词： Schedules Ciphers Analytical models Toy manufacturing industry Force Linear approximation Transforms

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Segment-wise learning control for trajectory tracking of robot manipulators under iteration-dependent periods

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Science China(Information Sciences) 2024年第3期67卷 155-166页

作者： fan zhang Deyuan MENG kaiquan CAI The Seventh Research Division Beihang University School of Automation Science and Electrical Engineering Beihang University School of Electronics and Information Engineering Beihang University

This paper is concerned with the amplitude boundedness problem of adaptive iterative learning control(AILC) for robot manipulators operating with iteration-dependent periods. By introducing virtual memory slots for storing historical data, a practical AILC method is proposed to achieve the segmentwise learning. This method requires less memory storage for historical information of previous iterations,especially in comparison with that of the conventional AILC methods using point-wise learning *** is shown that not only the energy boundedness but also the amplitude boundedness of estimates and inputs of practical AILC can be guaranteed. Moreover, the practical AILC method can achieve the perfect tracking objective regardless of iteration-dependent periods when the robot manipulators have a persistent full learning property. In addition, a solution to the visual manipulator platform is provided and deployed based on Coppeliasim and Matlab, which helps to show the amplitude boundedness of learning results and the perfect tracking performances of the proposed practical AILC method for robot manipulators.

关键词： amplitude boundedness iteration-dependent period iterative learning control robot manipulator segment-wise virtual memory slot

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Data-driven mapping-relationship mining between hardness and mechanical properties of dual-phase titanium alloys via random forest and statistical analysis

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Rare Metals 2024年第2期43卷 829-841页

作者： Hai-Chao Gong Qun-Bo fan Hong-Mei zhang Xing-Wang Cheng Wen-Qiang Xie kai Chen Lin Yang Jun-Jie zhang Bing-Qiang Wei Shun Xu School of Materials Science and Engineering Beijing Institute of TechnologyBeijing100081China National Key Laboratory of Science and Technology on Materials Under Shock and Impact Beijing100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing401135China Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNE68588USA

In order to accelerate the research on the property optimization of titanium alloy based on high-throughput methods,it is necessary to reveal the relationship between hardness and other mechanical properties which is still *** this work,taking Ti20C alloy as research object,almost all the microstructure of dual-phase titanium alloys were covered by traversing over 100 heat treatment ***,massive experiments including microstructure characterization and performance test were conducted,obtaining 51,590 pieces of microstructure data and 3591 pieces of mechanical property ***,based on large-scale data-driven technology,the quantitative mapping relationship between hardness and other mechanical properties was deeply *** results of random forest models showed that the correlation between hardness(H)and Charpy impact energy(A_(k))(or elongation,A)was hardly dependent on the microstructure types,while the relationship between H and tensile strength(R_(m))(or yield strength,R_(p0.2))was highly dependent on microstructure ***,combined with statistical analysis,it was found that the relationship between H and Ak(or A)were negatively ***,the relationship between H and strength was positively linear for equiaxed microstructure,and strength was linked to d^(−1/2)(d,equivalent circle diameter)ofα-grains in the form of classical Hall–Petch formula;but for other microstructures,the relationships were ***,the above rules were nearly the same in the rolling direction and transverse ***,a"four-quadrant partition map"between H and R_(p0.2)/R_(m) was established as a versatile material-screening tool,which can provide guidance for on-demand selection of titanium alloys.

关键词： Dual-phase titanium alloy Data-driven Hardness Mapping relationship

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Milling degree affects the fermentation properties of rice:perspectives from the composition of nutrients and gut microbiota via in vitro fermentation

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Food Science and Human Wellness 2024年第3期13卷 1578-1588页

作者： Yu zhang fan Li Shutong Pan Bing Bai kai Huang Sen Li Hongwei Cao Tian Xie Jian Xie Xiao Guan School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghai 200093China National Grain Industry(Urban Grain and Oil Security)Technology Innovation Center Shanghai 200093China Nutrition&Health Research Institute Co.Ltd. COFCO CorporationBeijing 102209China China Grain Wuhan Scientific Research&Design Institute Co.Ltd. Wuhan 430079China

Fermentation substrates of rice with different milling degrees(MDs) were prepared and fermented with human feces to compare their fermentation properties and effects on gut *** 0s,MD 5s and MD 60s represented brown rice,moderately-milled rice and white rice,*** in vitro fermentation,the MD 5s group showed higher starch utilization,compared with the MD 0s and 60s groups evaluated by Fourier transform infrared spectrometer,and confocal laser scanning *** of fermentation substrates of rice with different MDs on gut microbiota were evaluated by 16S rDNA *** the sample groups reduced the pH and produced short-chain fatty acids(SCFAs) and branched-chain fatty *** MD 5s group exhibited higher α-diversity than the MD 0s and 60s *** of Phascolarctobacterium,Blautia and norank_f_Ruminococcaceae were higher in the MD 0s and 5s groups,compared with the MD 60s *** bacteria were also positively correlated with the SCFAs production via Spearman correlation *** vitro culture assay revealed that fermentation substrates of MD 0s and 5s promoted the growth of two probiotics(Akkermansia muciniphila and Bifidobacterium adolescentis).Our results showed that moderate milling might be an appropriate way to produce rice products with richer nutrients and better fermentation properties.

关键词： Rice processing Milling Whole grains Gut microbiota

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A Single-Cell Landscape of Human Liver Transplantation Reveals a Pathogenic Immune Niche Associated with Early Allograft Dysfunction

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Engineering 2024年第5期36卷 193-208页

作者： Xin Shao Zheng Wang kai Wang Xiaoyan Lu Ping zhang Rongfang Guo Jie Liao Penghui Yang Shusen Zheng Xiao Xu Xiaohui fan Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang UniversityJiaxing 314103China Zhejiang University School of Medicine Hangzhou 310058China NHC Key Laboratory of Combined Multi-organ Transplantation Hangzhou 310003China Department of Hepatobiliary and Pancreatic Surgery Shulan(Hangzhou)Hospital Affiliated to Shulan International Medical CollegeZhejiang Shuren UniversityHangzhou 310000China Engineering Research Center of Innovative Anticancer Drugs Ministry of EducationHarbin 150023China

Liver transplantation(LT)is the standard therapy for individuals afflicted with end-stage liver *** notable advancements in LT technology,the incidence of early allograft dysfunction(EAD)remains a critical concern,exacerbating the current organ shortage and detrimentally affecting the prognosis of ***,the perplexing hepatic heterogeneity has impeded characterization of the cellular traits and molecular events that contribute to ***,we constructed a pioneering single-cell transcriptomic landscape of human transplanted livers derived from non-EAD and EAD patients,with 12 liver samples collected from 7 donors during the cold perfusion and portal reperfusion *** of the 75231 cells of non-EAD and EAD patients revealed an EAD-associated immune niche comprising mucosal-associated invariant T cells,granzyme B^(+)(GZMB^(+))granzyme K^(+)(GZMK^(+))natural killer cells,and S100 calcium binding protein A12^(+)(S100A12^(+))***,we verified this immune niche and its association with EAD occurrence in two independent *** findings elucidate the cellular characteristics of transplanted livers and the EAD-associated pathogenic immune niche at the single-cell level,thus,offering valuable insights into EAD onset.

关键词： Human liver transplantation Early allograft dysfunction Pathogenic immune niche Single-cell analysis Cell-cell communication

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Investigation of electrostatically tunable adhesion and instability of flying head slider

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Friction 2024年第3期12卷 462-473页

作者： fan zhang Yu WANG Yueqiang HU Mingquan zhang Baotong LI School of Mechanical Engineering Xi’an Jiaotong UniversityXi’an 710049China National Engineering Research Center for High Efficiency Grinding College of Mechanical and Vehicle EngineeringHunan UniversityChangsha 410082China

The interfacial adhesion between microstructures is inevitable in a micro-electro-mechanical system(e.g.,hard disk drive(HDD)),which may lead to complicated microtribodynamics *** research has investigated the effect of surface potential on the interfacial adhesion and microtribodynamics of the head–disk interface(HDI)in an HDD.A dynamic continuum surface force model,where the electrowetting is considered,is proposed to evaluate the interfacial interaction,and then employed into a two-degree-of-freedom(2DOF)model to theoretically analyze the potential influence mechanism on the *** results confirm that the elimination of potential can effectively repress the adhesion retention,which is further proved by the measured slider response with a laser Doppler vibrometer(LDV).Moreover,the effect of the potential on the adhesion-induced instability is also analyzed through the phase *** tells that the critical stable flying height can be lowered with the elimination of potential.

关键词： electric field(EF) retention instability adhesion

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Proposition of atomic and close-to-atomic scale manufacturing

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Advances in Manufacturing 2024年第1期12卷 1-5页

作者： Ju‑fan zhang Jens Ducree School of Mechanical and Materials Engineering University College DublinDublin 4Ireland School of Physical Sciences Dublin City UniversityDublinIreland

Atomic and close-to-atomic scale manufacturing(ACSM)is the core competence of Manufacturing *** other conceptions or terminologies that only focus on the atomic level precision,ACSM defnes a new realm of manufacturing where quantum mechanics plays the dominant role in the atom/molecule addition,migration and removal,considering the uncertainty principle and the discrete nature of *** ACSM is still in its infant stage,only little has been systematically elaborated at the core proposition of ACSM by now,hence there is a need to understand its concept and *** article elucidates the development of ACSM and clarifes its proposition,which aims to achieve a clearer understanding on ACSM and direct more efective eforts toward this promising area.

关键词： Atomic and close-to-atomic scale manufacturing(ACSM) Atomic Precision Manufacturing Paradigm Manufacturing III

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Testing Linearity in Functional Partially Linear Models

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Acta Mathematicae Applicatae Sinica 2024年第3期40卷 875-886页

作者： fan-rong ZHAO Bao-xue zhang School of Mathematical Sciences Shanxi UniversityTaiyuan 030006China School of Statistics Capital University of Economics and BusinessBeijing 100070China

For the functional partially linear models including flexible nonparametric part and functional linear part,the estimators of the nonlinear function and the slope function have been studied in existing *** to test the correlation between response and explanatory variables,however,still seems to be ***,a test procedure for testing the linearity in the functional partially linear models will be proposed in this paper.A test statistic is constructed based on the existing estimators of the nonlinear and the slope ***,we prove that the approximately asymptotic distribution of the proposed statistic is a chi-squared distribution under some regularity ***,some simulation studies and a real data application are presented to demonstrate the performance of the proposed test statistic.

关键词： asymptotic theory functional partially linear model Nadaraya-Watson estimate test statistic

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Establishing VIGS and CRISPR/Cas9 techniques to verify RsPDS function in radish

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Journal of Integrative Agriculture 2024年第5期23卷 1557-1567页

作者： Jiali Ying Yan Wang Liang Xu Tiaojiao Qin kai Xia Peng zhang Yinbo Ma Keyun zhang Lun Wang Junhui Dong Lianxue fan Yuelin Zhu Liwang Liu National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization/Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of Ministry of Agriculture and Rural Affairs/College of Horticulture Nanjing Agricultural UniversityNanjing210095China College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou 225009China College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China

Virus-induced gene silencing(VIGS)and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)systems are effective technologies for rapid and accurate gene function verification in modern plant ***,the investigation of gene silencing and editing in radish remains *** this study,a bleaching phenotype was generated through the knockdown of RsPDS using tobacco rattle virus(TRV)-and turnip yellow mosaic virus(TYMV)-mediated gene silencing *** TYMV-mediated gene silencing efficiency was higher than the TRV-based VIGS system in *** expression level of RsPDS was significantly inhibited using VIGS in'NAU-067'radish *** rootless seedlings of‘NAU-067'were infected with Agrobacterium rhizogenes using the 2300GN-Ubi-RsPDS-Cas9 vector with two target *** adventitious roots were blue with GUs staining,and four of these adventitious roots were edited at target sequence 1 of the RsPDS gene as indicated by Sanger ***,albino lines were generated with ***-mediated transformation of radish *** base substitutions and three base deletions occurred at target sequence 2 in Line 1,and three base insertions and three base substitutions occurred at target sequence 1 in Line *** study shows that VIGS and CRISPR/Cas9 techniques can be employed to precisely verify the biological functions of genes in radish,which will facilitate the genetic improvement of vital horticultural traits in radish breeding programs.

关键词： Raphanus sativus L. VIGS CRISPR/Cas9 Agrobacterium rhizogenes A.tumefaciens RsPDS

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