检索结果-南通市图书馆

KLF4 Inhibits the Activation of Human Hepatic Stellate Cell In Vitro

在线全文

学校读者我要写书评

暂无评论

Current Medical Science 2024年第3期44卷 512-518页

作者： Xing-yu YANG Zhe CHEN Jun TAN yin-kai XUE Hai ZHENG Department of Emergency Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Department of Thoracic Surgery The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an710061China Department of Thoracic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Objective Hepatic stellate cells(HSCs)play a crucial role in liver fibrosis.Early-stage liver fibrosis is reversible and intimately associated with the state of HSCs.Kruppel-like factor 4(KLF4)plays a pivotal role in a wide array of physiological and pathological processes.This study aimed to investigate the effect of KLF4 on the proliferation,apoptosis and phenotype of quiescent HSCs Methods We designed a KLF4 lentiviral vector and a KLF4 siRNA lentiviral vector,to upregulate and silence KLF4 expression in human HSC LX-2 cells via transfection.Cell proliferation was assessed using the CCK-8 assay.Flow cytometry was used to detect the cell cycle distribution and apoptosis rate.Western blotting was used to determine the levels of some quiescence and activation markers of HSCs Results Overexpression of KLF4 significantly increased the levels of E-cadherin and ZO-1,which are quiescent HSC markers,while significantly decreased the levels of N-cadherin and a-SMA,known activated HSC markers.In contrast,cell proliferation and apoptosis rates were elevated in LX-2 cells in which KLF4 expression was silenced Conclusion KLF4 inhibits the proliferation and activation of human LX-2 HSCs.It might be a key regulatory protein in the maintenance of HSC quiescence and may serve as a target for the inhibition of hepatic fibrosis.

关键词： Kruppel-like factor 4 hepatic stellate cells LX-2 cells liver fibrosis

Cardiac regeneration: Pre-existing cardiomyocyte as the hub of novel signaling pathway

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第2期11卷 747-759页

作者： Tao Wang Xinzhe Chen kai Wang Jie Ju Xue Yu Wanpeng Yu Cuiyun Liu yin Wang Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityCollege of MedicineQingdao UniversityQingdaoShandong 266023China College of Medicine Qingdao UniversityQingdaoShandong 266023China

In the mammalian heart,cardiomyocytes are forced to withdraw from the cell cycle shortly after birth,limiting the ability of the heart to regenerate and repair.The development of multimodal regulation of cardiac proliferation has verified that pre-existing cardiomyocyte proliferation is an essential driver of cardiac renewal.With the continuous development of genetic lineage tracking technology,it has been revealed that cell cycle activity produces polyploid cardiomyocytes during the embryonic,juvenile,and adult stages of cardiogenesis,but newly formed mononucleated diploid cardiomyocytes also elevated sporadically during myocardial infarction.It implied that adult cardiomyocytes have a weak regenerative capacity under the condition of ischemia injury,which offers hope for the clinical treatment of myocardial infarction.However,the regeneration frequency and source of cardiomyocytes are still low,and the mechanism of regulating cardiomyocyte proliferation remains further explained.It is noteworthy to explore what force triggers endogenous cardiomyocyte proliferation and heart regeneration.Here,we focused on summarizing the recent research progress of emerging endogenous key modulators and crosstalk with other signaling pathways and furnished valuable insights into the internal mechanism of heart regeneration.In addition,myocardial transcription factors,non-coding RNAs,cyclins,and cell cycle-dependent kinases are involved in the multimodal regulation of pre-existing cardiomyocyte proliferation.Ultimately,awakening the myocardial proliferation endogenous modulator and regeneration pathways may be the final battlefield for the regenerative therapy of cardiovascular diseases.

关键词： Cardiac regeneration Cell cycle re-enter Myocardial infarction Pre-existing cardiomyocyte proliferation Regenerative therapy of cardiovascular diseases

Searching for plant NLR immune receptors conferring resistance to potyviruses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2024年第1期12卷 28-44页

作者： Xin Hong Shufen Li Xiaofei Cheng Haijian Zhi Jinlong yin kai Xu Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for MicrobiologyCollege of Life SciencesNanjing Normal UniversityNanjing 210023JiangsuChina College of Plant Protection Northeast Agricultural UniversityHarbin 150030HeilongjiangChina National Center for Soybean Improvement National Key Laboratory for Crop Genetics and Germplasm EnhancementKey Laboratory of Biology and Genetic Improvement of Soybean-Ministry of AgricultureNanjing Agricultural UniversityNanjing 210095JiangsuChina

To fight against invasion by pathogens,plants have evolved an elaborate innate immune system,of which the nucleotide-binding domain leucine-rich repeat-containing receptor(NLR)acts as the sensor and immune executor.Potyviruses,comprising one of the largest genera of plant viruses,cause severe crop yield losses worldwide.Inherited crop resistance to potyviruses can be used in breeding and plant transgenesis to control disease development.This review summarizes achievements in mapping and cloning NLR genes conferring dominant resistance against potyvirus in the families Fabaceae,Solanaceae,Brassicaceae,and Cucurbitaceae.It compares mechanisms of potyviral protein recognition and downstream signaling employed by NLRs and discusses strategies for exploiting NLRs to better control diseases caused by potyviruses.

关键词： NLR Potyvirus resistance Genetic diversity Mapping Engineer

LONP1 ameliorates liver injury and improves gluconeogenesis dysfunction in acute-on-chronic liver failure

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2024年第2期137卷 190-199页

作者： Muchen Wu Jing Wu kai Liu Minjie Jiang Fang Xie Xuehong yin Jushan Wu Qinghua Meng Department of Liver Disease Beijing You-An HospitalCapital Medical UniversityBeijing 100069China Beijing Institute of Hepatology Beijing You-An HospitalCapital Medical UniversityBeijing 100069China Department of General Surgery Beijing You-An HospitalCapital Medical UniversityBeijing 100069China

Background:Acute-on-chronic liver failure(ACLF)is a severe liver disease with complex pathogenesis.Clinical hypoglycemia is common in patients with ACLF and often predicts a worse prognosis.Accumulating evidence suggests that glucose metabolic disturbance,especially gluconeogenesis dysfunction,plays a critical role in the disease progression of ACLF.Lon protease-1(LONP1)is a novel mediator of energy and glucose metabolism.However,whether gluconeogenesis is a potential mechanism through which LONP1 modulates ACLF remains unknown.Methods:In this study,we collected liver tissues from ACLF patients,established an ACLF mouse model with carbon tetrachloride(CCl 4),lipopolysaccharide(LPS),and D-galactose(D-gal),and constructed an in vitro hypoxia and hyperammonemia-triggered hepatocyte injury model.LONP1 overexpression and knockdown adenovirus were used to assess the protective effect of LONP1 on liver injury and gluconeogenesis regulation.Liver histopathology,biochemical index,mitochondrial morphology,cell viability and apoptosis,and the expression and activity of key gluconeogenic enzymes were detected to explore the underlying protective mechanisms of LONP1 in ACLF.Results:We found that LONP1 and the expressions of gluconeogenic enzymes were downregulated in clinical ACLF liver tissues.Furthermore,LONP1 overexpression remarkably attenuated liver injury,which was characterized by improved liver histopathological lesions and decreased serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in ACLF mice.Moreover,mitochondrial morphology was improved upon overexpression of LONP1.Meanwhile,the expression and activity of the key gluconeogenic enzymes were restored by LONP1 overexpression.Similarly,the hepatoprotective effect was also observed in the hepatocyte injury model,as evidenced by improved cell viability,reduced cell apoptosis,and improved gluconeogenesis level and activity,while LONP1 knockdown worsened liver injury and gluconeogenesis disorders.Conclusion:We demonstrated that gluconeogenesis dysfunc

关键词： Acute-on-chronic liver failure Lon protease-1 Gluconeogenesis disturbance Mitochondrial dysfunction Metabolism

Solid particle size characterization by a high-frequency collision response in pneumatic particulate flow

在线全文

学校读者我要写书评

暂无评论

Particuology 2024年第3期86卷 39-54页

作者： kai Wang Yichen Li Ziang Chang Min Qin Guangming Fu Bangtang yin Gang Wang jiaqi Tian School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580China Key Laboratory of Unconventional Oil&Gas Development China University of Petroleum(East China)Ministry of EducationQingdao266580China National Engineering Research Center of Oil&Gas Drilling and Completion Technology Qingdao266580China Shock and Vibration of Engineering Materials and Structures Key Lab ofSichuan Province Southwest University of Science and TechnologyMianyang621000China

A novel triaxial vibration method is developed for the real-time characterization of the solid particle size distribution(PsD)in pneumatic particulate flow,which is critical for chemical industry.In this work,the particle-wall collision and friction behaviours were analysed by the time-domain statistical and timefrequency joint methods to narrow the high-frequency response range by the initial experiment of free fall for a single particle,interparticle,and multiple particles.Subsequently,verification experiments of PSD characterization in pneumatic flow were performed.First,the quantitative triaxial energy response model that considers the particle size,shape,and mass factors were established.Second,a good agreement of the particle number identification was found between the triaxial vibration energy and mean particle size of 150-550μm.Moreover,the performance with the best accuracy was focused on a range of 42-43 kHz in the x-axis and z-axis and 36.8-38.8 kHz in the y-axis.Finally,the individual particle energy was inversely analysed by the triaxial vibration response within the optimized frequency bands,and the PSD was characterized in real-time by a low error rate,that is,5.2% from the XZ-axis direction of sand(42-43 kHz)and 5.6% from the XYZ-axis of glass(30.9-33.9 kHz,46.2-47.2 kHz,38.3-41.3 kHz for each axis response).Therefore,this research complements the existing approaches for PsD characterization in particulate multiphase flow.

关键词： Solid particulate flow Triaxial vibration High-frequency response Particle wall Particle size distribution

Variation in Bombyx mori immune response against fungal pathogen Beauveria bassiana with variability in cell wallβ-1,3-glucan

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2024年第1期31卷 211-224页

作者： Yulong Wang Zhen Liu Xuebing yin Shihong Liu kai Wang Rongjie Wan Haoran Chen Xinyang Li Bo Huang Anhui Provincial Key Laboratory of Microbial Pest Control Anhui Agricultural UniversityHefeiChina

Entomopathogenic fungi are protected by a cell wall with dynamic structure for adapting to various environmental conditions.β-1,3-Glucan recognition proteins activate the innate immune system of insects by recognizing surface molecules of fungi.However,the associations between pathogenicity and the different components of entomopathogenic fungal cell walls remain unclear.Three Beauveria bassiana strains were selected that have significantly differing virulence against Bombyx mori.The molecular mechanisms underlying the immune response in B.mori were investigated using RNA sequencing,which revealed differences in the immune response to different B.bassiana strains at 12 h post-infection.Immunofluorescence assays revealed thatβ-1,3-glucan content had an opposite trend to that of fungal virulence.β-1,3-Glucan injection upregulated BmβGRP4 expression and significantly reduced the virulence of the high-virulence strain but not that of the medium-virulence or low-virulence strains.BmβGRP4 silencing in B.mori with RNA interference resulted in the opposite virulence pattern,indicating that the virulence of B.bassiana was affected by the cell walls’content ofβ-1,3-glucan,which could be recognized by BmβGRP4.Furthermore,interference with the gene CnA(calcineurin catalytic A subunit)involved inβ-1,3-glucan synthesis eliminated differences in virulence between B.bassiana strains.These results indicate that strains of a single species of pathogenic fungi that have differing cell wall components are recognized differently by the innate immune system of B.mori.

关键词： ombyx mori BmβGRP4 innate immunity insect fungal pathogen virulence

Diabetes mellitus in patients with type 1 autoimmune pancreatitis at diagnosis and after corticosteroid therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2024年第4期23卷 393-398页

作者： Mei-Zi Li Tao Guo Yun-Lu Feng Sheng-Yu Zhang Xiao-yin Bai Xi Wu kai Xu Ai-Ming Yang Department of Gastroenterology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China Department of Radiology Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China

Background:A high prevalence of diabetes mellitus(DM)coexisting with autoimmune pancreatitis(AIP)is observed.However,evidence on the circumstances under which corticosteroid therapy(CST)for AIP improves or worsens DM is scarce.This study aimed to demonstrate and identify predictors of DM control under the influence of CST.Methods:Patients diagnosed with type 1 AIP were enrolled from a prospectively maintained cohort and were classified into three groups according to the chronology in which AIP and DM were diagnosed:pre-existing DM(pDM),concurrent DM(cDM),and non-DM(nDM).The responses of DM to CST were assessed when corticosteroid was ceased or tapered to a maintenance dose and classified as‘improvement’and‘non-improvement’(including‘no change’and‘exacerbation’).Results:Among 101 patients with type 1 AIP,52(51.5%)patients were complicated with DM at the time of AIP diagnosis,with 36 patients in the cDM group and 16 patients in the pDM group.The incidences of diffuse pancreatic swelling(72.2%)and pancreatic body/tail involvement(91.7%)were significantly higher in the cDM group than in both the pDM and nDM groups.Of the 52 patients with DM,CST was administered in 48 cases.Multivariate logistic analysis identified that elevated serum gamma-glutamyl transferase(GGT)level at AIP diagnosis[odds ratio(OR)=0.032,95%confidence interval(CI):0.003-0.412,P=0.008]and pancreatic atrophy after CST(OR=0.027,95%CI:0.003-0.295,P=0.003)were negatively associated with DM control improvement.Conclusions:Patients with diffuse pancreatic swelling and pancreatic body/tail involvement in pancreatitis tended to be complicated with cDM at AIP diagnosis.CST exerted a beneficial effect on the clinical course of DM in nearly half of the AIP patients complicated with DM at diagnosis,particularly in those without elevated serum GGT levels at diagnosis and who did not experience pancreatic atrophy after CST.

关键词： Type 1 autoimmune pancreatitis Diabetes mellitus Corticosteroid therapy Predictive factor Pancreatic atrophy

Quantum Neural Network for Quantum Neural Computing

在线全文

学校读者我要写书评

暂无评论

Research 2024年第1期2023卷 71-80页

作者： Min-Gang Zhou Zhi-Ping Liu Hua-Lei yin Chen-Long Li Tong-kai Xu Zeng-Bing Chen National Laboratory of Solid State Microstructures School of PhysicsCollaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China MatricTime Digital Technology Co.Ltd. Nanjing 211899China

Neural networks have achieved impressive breakthroughs in both industry and academia.How to effectively develop neural networks on quantum computing devices is a challenging open problem.Here,we propose a new quantum neural network model for quantum neural computing using(classically controlled)single-qubit operations and measurements on real-world quantum systems with naturally occurring environment-induced decoherence,which greatly reduces the difficulties of physical implementations.Our model circumvents the problem that the state-space size grows exponentially with the number of neurons,thereby greatly reducing memory requirements and allowing for fast optimization with traditional optimization algorithms.We benchmark our model for handwritten digit recognition and other nonlinear classification tasks.The results show that our model has an amazing nonlinear classification ability and robustness to noise.Furthermore,our model allows quantum computing to be applied in a wider context and inspires the earlier development of a quantum neural computer than standard quantum computers.

关键词： quantum thereby breakthrough

Sensing as the key to the safety and sustainability of new energy storage devices

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protection and Control of Modern Power Systems 2023年第2期8卷 185-206页

作者： Zhenxiao Yi Zhaoliang Chen kai yin Licheng Wang kai Wang School of Electrical Engineering Weihai Innovation Research InstituteQingdao UniversityQingdao 266000China Qingdao No.2 Middle School Qingdao 266199China Hunan Key Laboratory of Nanophotonics and Devices School of Physics and ElectronicsCentral South UniversityChangsha 410083China School of Information Engineering Zhejiang University of TechnologyHangzhou 310014China

New energy storage devices such as batteries and supercapacitors are widely used in various fields because of their irreplaceable excellent characteristics.Because there are relatively few monitoring parameters and limited under-standing of their operation,they present problems in accurately predicting their state and controlling operation,such as state of charge,state of health,and early failure indicators.Poor monitoring can seriously affect the performance of energy storage devices.Therefore,to maximize the efficiency of new energy storage devices without damaging the equipment,it is important to make full use of sensing systems to accurately monitor important parameters such as voltage,current,temperature,and strain.These are highly related to their states.Hence,this paper reviews the sensing methods and divides them into two categories:embedded and non-embedded sensors.A variety of measurement methods used to measure the above parameters of various new energy storage devices such as batteries and super-capacitors are systematically summarized.The methods with different innovative points are listed,their advantages and disadvantages are summarized,and the application of optical fiber sensors is emphasized.Finally,the challenges and prospects for these studies are described.The intent is to encourage researchers in relevant fields to study the early warning of safety accidents from the root causes.

关键词： New energy storage devices Battery Supercapacitor Embedded sensors Non-embedded sensors Sensing