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Transcriptome of Erysiphe necator-infected Vitispseudoreticulata leaves provides insight into grapevineresistance to powdery mildew

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Horticulture Research 2014年第1期1卷 86-97页

作者： kai weng Zhi-Qian Li Rui-Qi Liu Lan Wang Yue-Jin Wang Yan Xu State Key Laboratory of Crop Stress Biology in Arid Areas(Northwest A&F University) Yanglingshanxi 712100China College of Horticulture Northwest A&F UniversityYanglingshanxi 712100China Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China Ministry of AgricultureYanglingshanxi 712100China

Powdery mildew(PM),which is caused by the pathogen Erysiphe necator(Schw.)Burr.,is the single most damaging disease of cultivated grapes(Vitis vinifera)***,little is known about the transcriptional response of grapes to infection with ***-seq analysis was used for deep sequencing of the leaf transcriptome to study PM resistance in Chinese wild grapes(*** Baihe 35-1)to better understand the interaction between host and *** than 100 million(M)90-nt cDNA reads were sequenced from a cDNA library derived from PM-infected *** the sequences obtained,6541 genes were differentially expressed(DEG)and were annotated with Gene Ontology terms and by pathway *** significant categories that were identified included the following:defense,salicylic acid(SA)and jasmonic acid(JA)responses;systemic acquired resistance(SAR);hypersensitive response;plant–pathogen interaction;flavonoid biosynthesis;and plant hormone signal *** putative secretory proteins were identified,indicating potential defense responses to *** all,318 putative R-genes and 183 putative secreted proteins were identified,including the defense-related R-genes BAK1,MRH1 and MLO3 and the defense-related secreted proteins GLP and *** expression patterns of 16 genes were further illuminated by *** present study identified several candidate genes and pathways that may contribute to PM resistance in grapes and illustrated that RNA-seq is a powerful tool for studying gene *** RT-qPCR results reveal that effective resistance responses of grapes to PM include enhancement of JA and SAR responses and accumulation of phytoalexins.

关键词： resistance Vitis interaction

Zircon U-Pb ages in the Nuratau ophiolitic mélange in the southern Tianshan,Uzbekistan:Implication for the closure of Paleo-Asian Ocean

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China Geology 2024年第2期7卷 365-368,I0004-I0007页

作者： kai weng Ji-fei Cao Divayev-Farid Karibovich Jahongir-Jurabekovich Movlanov Bo Chen Zhong-ping Ma Xi’an Center of Geological Survey China Geological SurveyXi’an 710119China The Center for Geoinnovation Technologies Doctor of Sciences in Geology and Mineralogy University of Geological ScienceTashkent 100060Uzbekistan

*** The Central Asian Orogenic Belt(CAOB),which is located between the European craton,Siberian craton,and Tarim-North China craton(Fig.1a),is the largest phanerozoic accretionary orogen in the *** resulted from the longterm subduction and accretion of the Paleo-Asian Ocean(PAO).The PAO has been in existence since at least the late Mesoproterozoic(about 1020 Ma).However,there has been debate about the closing time of the PAO.

关键词： Asian Paleo Tianshan

LA-ICP-MS zircon U-Pb dating of tuffites in the Sachakou Pb-Zn mining area, Karakorum, Xinjiang and its establishment of Early Triassic strata

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China Geology 2024年第1期7卷 150-152页

作者： Xiao-jian Zhao Nuo Li kai weng Ming Wang Qing-lin Sui Deng-hui Chen Zhou-ping Guo Meng-qi Jin Xi’an Center China Geological SurveyMinistry of Natural ResourcesXi’an 710054China Xinjiang Research Center for Mineral Resources Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China College of Geography and Remote Sensing Sciences Xinjiang UniversityUrumqi 830046China University of Chinese Academy of Sciences Beijing 100029China No.8 Geological Party Xinjiang Bureau of Geology and Mineral Exploration and DevelopmentAksu 843000China School of Resource&Environment and Safety Engineering University of South ChinaHengyang 421001China

*** In the past decade,a group of medium to giant lead-zinc deposits,represented by Huoshaoyun,Sachakou,and Yuanbaoling,have been discovered in the Aksai Chin region of Karakoram,*** are all located in the Mesozoic carbonate and clastic rock *** Sachakou leadzinc mining area is adjacent to the northwest of the Huoshaoyun lead-zinc mining area and is in the same stratigraphic layer as *** many scholars have been arguing about the type and age of Huoshaoyun lead-zinc mineralization,few scholars have paid attention to the classification of the ore-bearing strata in the *** stratigraphy of the Lower Permian Shenxianwan Group to the Upper Cretaceous Tielongtan Group is exposed in the Sachakou area of Karakorum,Xinjiang,however,the Late Permian-Early Triassic stratigraphy is missing(Fig.1a).Due to the harsh natural conditions in the area and the low level of work,the stratigraphic delineation is not exhaustive,and the regional lithology is dominated by carbonates and clastic rocks,which makes it difficult to identify the age of the regional lithology and causes problems for the exploration and research of lead-zinc in the area.

关键词： strata Triassic Xinjiang

基于BIM正向设计的铁路站房标准化体系建设

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铁道工程学报 2023年第12期40卷 88-94页

作者：翁凯李俊生中国铁路设计集团有限公司天津300308

研究目的:BIM正向设计是设计企业数字化转型的重要抓手,但现阶段铁路站房BIM正向设计的落地实施及推广应用存在种种问题,而标准化有助于规范设计企业BIM技术的应用,对推动BIM正向设计的技术发展有着指引作用。为此,本文从标准化入手,从... 详细信息

研究目的:BIM正向设计是设计企业数字化转型的重要抓手,但现阶段铁路站房BIM正向设计的落地实施及推广应用存在种种问题,而标准化有助于规范设计企业BIM技术的应用,对推动BIM正向设计的技术发展有着指引作用。为此,本文从标准化入手,从设计标准指引、标准设计流程、标准设计资源、标准执行、标准设计应用五方面构建铁路站房BIM正向设计标准化体系。研究结论:(1)完善的BIM正向设计标准化体系是设计企业BIM技术推广应用的重要因素,有助于提升BIM设计及应用水平;(2)研究实现了标准构件、标准详图族、标准建模深度、标准视图样板、标准提资、标准视图浏览器等标准化应用,形成了从建模、计算、提资、出图、工程量统计、可视化应用的全过程解决方案;(3)铁路站房BIM标准化体系在凌海南站中应用,实现了全过程全流程标准化,提升了设计质量和效率,并在其他项目中复用;(4)本研究结论可为铁路站房BIM正向设计推广及设计标准化提供参考。

关键词： BIM正向设计铁路站房标准化建设设计标准设计流程

Dynamic Behaviors of a Moored Floating Structure with Flexible Skirts

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Journal of Energy and Power Engineering 2016年第1期10卷 13-22页

作者： Wen-kai weng Duc-Tru Tran Ruey-Syan Shih Research Center for Ocean Energy and Strategies National Taiwan Ocean University Keelung 202 Taiwan R.O.C. Ph.D. Program in Department of Harbor and River Engineering National Taiwan Ocean University Keelung 202 Taiwan R.O.C. Department of Construction and Spatial Design. Tungnan University New Taipei City 222 Taiwan R.O.C

A numerical model was developed by using the dual boundary element method to investigate the dynamic behavior of a moored floating structure with a pair of vertical and flexible skirts attached at its bottom in the linear wave field. Theoretical conception is based on potential theory with linear external forces. The motions of the structure were assumed to be small and linear. The flexible skirts mounted beneath the structure were assumed uniform flexural rigidity and the thickness of the skirts was negligible. Comparison between the present model and Gesraha＇s solution was made to verify the results for a moored floating structure with or without rigid skirts. The influence of the skirt rigidity on the moored floating structure, moored lines and waves is investigated in this study. The results show that, the natural frequencies of structure＇s oscillation, moored force, wave reflection and transmission tend to the region of short-period waves when the flexible rigidity gradually decreases. Positive correlation exists between the aft mooring force and the pitch motion of the floating structure.

关键词： Floating structure dual boundary element method response amplitude operators flexible skirts.

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叶片尾部形状对双向贯流式水轮机性能的影响

农业工程学报 2015年第6期31卷 76-81页

作者：郑小波翁凯王玲军西安理工大学水利水电学院西安710048

为了研究双向贯流式水轮机反向工况效率低下的问题,该文以某带有后置导叶的双向贯流式机组为对象,针对不同形状和不同厚度的叶片尾部,分析了反向工况下叶片尾部对机组性能的影响。采用UG建模软件对机组进行几何建模,基于CFX软件,采用SST... 详细信息

为了研究双向贯流式水轮机反向工况效率低下的问题,该文以某带有后置导叶的双向贯流式机组为对象,针对不同形状和不同厚度的叶片尾部,分析了反向工况下叶片尾部对机组性能的影响。采用UG建模软件对机组进行几何建模,基于CFX软件,采用SST k-ω湍流模型对不同形状和厚度的叶片尾部的转轮进行了数值模拟。结果表明:反向工况下采用圆形尾部的叶片其机组效率为59.55%,高于矩形尾部的58.4%和弧形尾部的58.01%,说明反向工况下矩形尾部和弧形尾部的冲击损失较大。增加叶片尾部厚度对机组反向工况的效率提高较为明显,其效率最高能抬高到79%,但叶片尾部厚度增加到一定程度后效率不再增加,叶片尾部厚度的增加使得反向工况下叶片尾部最低压力值降低了1.2×106 Pa,对其反向工况下的空化性能有较大影响,且增加了正向工况运行是出现卡门涡的概率。研究成果为双向贯流式水轮机反向工况下叶片尾部形状的优化设计提供了经验参考。

关键词：水轮机效率模型反向工况叶片尾部

基于双向流固耦合的贯流式水轮机动力特性分析

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农业工程学报 2016年第4期32卷 78-83页

作者：郑小波王玲军翁凯西安理工大学水利水电学院西安710048 富安水力机械研究所杭州310030

为了深入研究流固耦合作用对贯流式水轮机转轮动力特性及内部流场的影响,文中采用商业软件CFX和ANSYS APDL对贯流式水轮机流体域和固体域进行耦合求解,分析了耦合作用对结构应力及应变的影响,并将耦合数值计算得到的转轮外特性与实测值... 详细信息

为了深入研究流固耦合作用对贯流式水轮机转轮动力特性及内部流场的影响,文中采用商业软件CFX和ANSYS APDL对贯流式水轮机流体域和固体域进行耦合求解,分析了耦合作用对结构应力及应变的影响,并将耦合数值计算得到的转轮外特性与实测值进行了对比。结果表明:考虑耦合作用后,转轮的效率、水头与耦合前相比都有不同程度的下降,最大值分别为0.6%、0.21 m。同时在靠近叶片出水边轮缘附近,耦合后压力面与吸力面压力差有所下降,说明耦合作用会降低转轮的水力性能。2种耦合计算方法求解得到的叶片的等效应力分布基本一致,应力集中都出现在转轮叶片与枢轴法兰联接处,同时双向耦合下最大等效应力的主频与单向耦合相比有明显下降的趋势,由于双向耦合考虑了结构在运动过程中周围水体与结构的相互影响。该研究为实际工程中准确地进行转轮的水力性能预估和叶片结构在水中瞬态响应计算提供了参考。

关键词：贯流式水轮机流场效率流固耦合等效应力变形位移压力分布

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鄂尔多斯盆地西南部蓟县系古油藏沥青

石油勘探与开发 2011年第2期38卷 168-173页

作者：李荣西梁积伟翁凯长安大学地球科学与资源学院长安大学西部矿产资源与工程地质教育部重点实验室

鄂尔多斯盆地西南缘蓟县系含硅质条带纹层状藻白云岩野外露头发现大量集中分布的天然固体沥青,沥青主要分布在蓟县系且自下而上穿过其与上覆下白垩统罗汉洞组界线进入到罗汉洞组紫红色砂泥岩中。沥青呈薄片状或脉状分布在岩石层面、孔... 详细信息

鄂尔多斯盆地西南缘蓟县系含硅质条带纹层状藻白云岩野外露头发现大量集中分布的天然固体沥青,沥青主要分布在蓟县系且自下而上穿过其与上覆下白垩统罗汉洞组界线进入到罗汉洞组紫红色砂泥岩中。沥青呈薄片状或脉状分布在岩石层面、孔隙和裂隙中。氯仿沥青"A"正构烷烃分布特征和萜烷、甾烷生物标志化合物分析表明,沥青以低等水生生物和藻类有机质为母源,蓟县系纹层状藻白云岩是其可能的烃源岩。沥青反射率为2.7%～3.6%,与研究区延安组煤层(镜质体反射率为0.6%～0.8%)热演化程度极不协调,表明其受控于局部热异常事件。早白垩世晚期铜城碱性岩体侵入作用导致了蓟县系古油藏破坏而形成了古油藏沥青。研究区具有油气形成的物质基础和聚集条件,鄂尔多斯盆地西南部蓟县系生烃潜力及其油气勘探前景值得重视和进一步深入研究。

关键词：古油藏沥青蓟县系油气勘探鄂尔多斯盆地

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锈蚀檩条在台风作用下时变可靠度计算模型

北京交通大学学报 2019年第6期43卷 67-74页

作者：刘威杨娜白凡翁凯北京交通大学土木建筑工程学院北京100044 中国铁路设计集团有限公司建筑设计研究院天津30014211

基于大气锈蚀模型和简支檩条的屈曲计算理论,结合时变可靠度方法,提出了锈蚀檩条在台风作用下的可靠度计算模型.该模型考虑了在抗力层次锈蚀导致的抗力退化,以及在荷载层次台风的发生次数及强度.结合算例研究了中国沿海11个城市的锈蚀... 详细信息

基于大气锈蚀模型和简支檩条的屈曲计算理论,结合时变可靠度方法,提出了锈蚀檩条在台风作用下的可靠度计算模型.该模型考虑了在抗力层次锈蚀导致的抗力退化,以及在荷载层次台风的发生次数及强度.结合算例研究了中国沿海11个城市的锈蚀檩条可靠度变化规律,通过给出服役期间的可靠指标,进行可靠性分析.结果表明:锈蚀檩条在台风作用下的可靠指标在前5年下降速率较快,在第5年至服役期满逐渐趋于平缓.在服役前5年,锈蚀作用影响较小,但服役5年后直到服役期满,其对檩条可靠指标的影响逐渐增大.对比中美两国不同目标可靠指标下的评估结果,发现按照中国规范的评估结果较为保守.

关键词：檩条时变可靠度锈蚀台风抗力退化