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基于柔性电极和信号放大技术构建检测磷脂酶C的电化学传感器

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应用化学 2024年第7期41卷 987-997页

作者：王瑶瑶孙铭阳杨钻杜绍恺何禹璇孙越辽宁师范大学化学化工学院大连116029

磷脂酶C(PLC)是动植物中重要的脂质水解酶,在哺乳动物细胞的代谢、生长、炎症、分化、衰老和凋亡中起着重要作用,与许多癌症的发生、发展显著相关。蛋白质催化原子转移自由基聚合(PATRP)是高分子设计、制备的有效手段,可通过链式反应将... 详细信息

磷脂酶C(PLC)是动植物中重要的脂质水解酶,在哺乳动物细胞的代谢、生长、炎症、分化、衰老和凋亡中起着重要作用,与许多癌症的发生、发展显著相关。蛋白质催化原子转移自由基聚合(PATRP)是高分子设计、制备的有效手段,可通过链式反应将数百个电活性单体连接成一个高相对分子质量的聚合物链,从而使分子识别信号大大增强。首先,在柔性碳布(CC)电极表面修饰纳米金(AuNPs),之后通过3-巯基丙酸、碳二亚胺盐酸盐和N-羟基丁二酰亚胺,将PLC的特异性底物磷脂酰乙醇胺(PE)固定在电极上,经过PLC对PE的特异性水解产生磷酸基团,引入Zr^(4+),通过Zr^(4+)的配位作用将聚合引发剂α-溴苯乙酸修饰到电极表面,最后在蛋白质催化作用下进行2,2,6,6-四甲基哌啶氧化物(TEMPO)的PATRP聚合反应,在电极表面引入电活性聚合物分子链,构建信号放大型电化学传感器P(TEMPO)/PE/AuNPs/CC,用于磷脂酶C的高灵敏度检测。结果表明,该传感器检测PLC的线性范围为10~1×10^(5)mU/L,检出限为3.568 mU/L(S/N=3)。与其他检测PLC的方法相比,该传感器具有较宽的检测范围和较低的检出限,且在检测实际乳腺癌细胞中的PLC方面有巨大的实际应用潜力。

关键词：磷脂酶C 原子转移自由基聚合柔性电极电化学传感器

Precise orbit determination for TH02-02 satellites based on BDS3 and GPS observations

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Chinese Journal of Aeronautics 2023年第5期36卷 475-485页

作者： Houzhe ZHANG Bing JU Defeng GU Ying LIU kai shao Xiaojun DUAN Zhiyong HUANG College of Science National University of Defense TechnologyChangsha 410073China National Key Laboratory of Science and Technology on Aerospace Flight Dynamics Beijing Aerospace Control CenterBeijing 100094China MOE Key Laboratory of TianQin Mission TianQin Research Center for Gravitational PhysicsFrontiers Science Center for TianQinCNSA Research Center for Gravitational WavesSun Yat-sen University(Zhuhai Campus)Zhuhai 519082China School of Artificial Intelligence Sun Yat-sen University(Zhuhai Campus)Zhuhai 519082China School of Physics and Astronomy Sun Yat-sen University(Zhuhai Campus)Zhuhai 519082China Information Engineering University Zhengzhou 450001China

The Tianhui-202(TH02-02)satellite formation,as a supplement to the microwave mapping satellite system Tianhui-201(TH02-01),is the first Interferometric Synthetic Aperture Radar(InSAR)satellite formation-flying system that supports the tracking of BeiDou global navigation Satellite system(BDS3)new B1C and B2a ***,the twin TH02-02 satellites also support the tracking of Global Positioning System(GPS)L1&L2 and BDS B1I&B3I *** the spaceborne receiver employs two independent boards to track the Global Navigation Satellite System(GNSS)satellites,we design an orbit determination strategy by estimating independent receiver clock offsets epoch by epoch for each GNSS to realize the multi-GNSS data fusion from different *** performance of the spaceborne receiver is evaluated and the contribution of BDS3 to the kinematic and reduced-dynamic Precise Orbit Determination(POD)of TH02-02 satellites is *** tracking data onboard shows that the average number of available BDS3 and GPS satellites are 8.7 and 9.1,*** carrier-to-noise ratio and carrier phase noise of BDS3 B1C and B2a signals are comparable to those of ***,strong azimuth-related systematic biases are recognized in the pseudorange multipath errors of B1C and *** pseudorange noise of BDS3 signals is better than that of GPS after eliminating the multipath errors from specific *** the GPS-based reduced-dynamic orbit with single-receiver ambiguity fixing technique as a reference,the results of BDS3-only and BDS3+GPS combined POD are *** Root Mean Square(RMS)of orbit comparison of BDS3-based kinematic and reduced-dynamic POD with reference orbit are better than 7 cm and 3 cm in three-Dimensional direction(3D).The POD performance based on B1C&B2a data is comparable to that based on B1I&*** precision of BDS3+GPS combined kinematic orbit can reach up to 3 cm(3D RMS),which has a more than 25%improvement relative to the GPS-only *** addi

关键词： BDS3 Multi-GNSS Precise orbit determination Spaceborne GNSS receiver TH02-02

MicroRNA-363-3p inhibits colorectal cancer progression by targeting interferon-induced transmembrane protein 1

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World Journal of Gastrointestinal Oncology 2023年第9期15卷 1556-1566页

作者： Yun Wang shao-kai Bai Tao Zhang Cheng-Gong Liao Department of Oncology Tangdu HospitalAir Force Medical UniversityXi’an 710038Shaanxi ProvinceChina

BACKGROUND The molecular mechanisms of colorectal cancer development and progression are far from being *** To investigate the role of microRNA-363-3p(miR-363-3p)in the progression of colorectal *** Real-time polymerase chain reaction was performed to detect miRNA expression in human colorectal cancer tissues and paired normal colorectal *** 6 was utilized to predict the targets of ***-luciferase reporter system was used to validate the target of *** colony formation assay and wound-healing assay were performed to evaluate cancer cells’clonogenic survival ability and migration ability,*** proliferation was examined by cell counting kit-8 *** staining was used to determine the expression level of interferon-induced transmembrane protein 1(IFITM1)in colorectal cancer tissues and adjacent *** TCGA and GTEx databases were used to compare the expression levels of IFITM1 mRNA in colorectal cancer tissues and normal colorectal tissues and analyze the correlation between the expression levels of IFITM1 mRNA and overall survival and disease-free survival of patients.A colorectal cancer cell line with a deficiency of IFITM1 was constructed,and the regulation effect of IFITM1 on the clonogenic growth of colorectal cancer cells was *** MiR-363-3p was decreased in colorectal cancer tissues compared to normal colorectal ***1 was characterized as a direct target of *** of miR-363-3p led to decreased clonogenic survival,proliferation,and migration of colorectal cancer cells,which could be reversed by forced IFITM1 *** MiR-363-3p can constrain clonogenic survival,proliferation,and migration of colorectal cancer cells via targeting IFITM1.

关键词： MicroRNA-363-3p Proliferation Clonogenic survival Colorectal cancer Interferon-induced transmembrane protein 1

Green Hydrothermal Synthesis and Applications of Sorbus pohuashanensis/Aronia melanocarpa Extracts Functionalized-Au/Ag/AuAg Nanoparticles

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Journal of Renewable Materials 2023年第4期11卷 1807-1821页

作者： Jin Huang Jixiang Sun kai shao Yamei Lin Zhiguo Liu Yujie Fu Liqiang Mu College of Chemistry Chemical Engineering and Resource UtilizationKey Laboratory of Forest Plant Ecology Ministry of EducationNortheast Forestry UniversityHarbin150040China School of Forestry Northeast Forestry UniversityHarbin150040China Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-Based Active Substances Harbin150040China National Engineering Laboratory of Bio-Resource Eco-Utilization Harbin150040China

Nanoparticles(NPs)have already been widely used in catalysis,antibacterial and coating *** with the traditional toxic and harmful reducing reagents,green synthesis of NPs by using plant extracts is not only environmental-friendly and cost-effective but also conducive to the multi-level and efficient utilization of wild plant *** this study,the aqueous extracts from Sorbus pohuashanensis(SP)and Aronia melanocarpa(AM)fruits were used as the reducing and protective reagents for synthesizing Au/AgNPs,with the characteristics of originality operation and high *** SP/AM fruit extracts functionalized Au/AgNPs were characterized by UV-vis spectroscopy(UV-vis),transmission electron microscopy(TEM),energy dispersive spectroscopy(EDS),scanning electron microscope(SEM),X-ray diffraction(XRD)and Fourier transform infrared spectroscopy(FTIR).UV-vis spectrums showed the NPs peaks verified by the presence between 400–550 nm;TEM and SEM demonstrated NPs displayed approximately spherical structures;EDS confirmed the existence of Au/Ag elements;XRD measurements confirmed that the obtained NPs showed highly crystalline structures;FTIR demonstrated the fruits extracts were adsorbed on the surface of *** experiments indicated that SP/AM fruit extracts functionalized-NPs could be used as the reagents for removing the organic dyes efficiently;Zone of inhibition tests(ZOI)explained that NPs have slow-release antibacterial activity.

关键词： Green synthesis nanoparticles Sorbus pohuashanensis Aronia melanocarpa plant extracts dye removal

Progress in blood biomarkers of subjective cognitive decline in preclinical Alzheimer’s disease

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Chinese Medical Journal 2023年第5期136卷 505-521页

作者： Xianfeng Yu kai shao Ke Wan Taoran Li Yuxia Li Xiaoqun Zhu Ying Han Department of Neurology Xuanwu Hospital of Capital Medical UniversityBeijing 100053China Department of Neurology The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230022China Department of Neurology Tangshan Central HospitalTangshanHebei 063000China School of Biomedical Engineering Hainan UniversityHaikouHainan 570228China Center of Alzheimer’s Disease Beijing Institute for Brain DisordersBeijing 100053China National Clinical Research Center for Geriatric Diseases Beijing 100053China.

Alzheimer's disease(AD)is a neurodegenerative disease that gradually impairs cognitive ***,there has been a conceptual shift toward AD to view the disease as a *** AD is currently incurable,effective intervention to delay or prevent pathological cognitive decline may best target the early stages of symptomatic disease,such as subjective cognitive decline(SCD),in which cognitive function remains relatively *** methods for identifying AD,such as cerebrospinal fluid biomarkers and positron emission tomography,are invasive and ***,it is imperative to develop blood biomarkers that are sensitive,less invasive,easier to access,and more cost effective for AD *** review aimed to summarize the current data on whether individuals with SCD differ reliably and effectively in subjective and objective performances compared to cognitively normal elderly individuals,and to find one or more convenient and accessible blood biomarkers so that researchers can identify SCD patients with preclinical AD in the population as soon as *** to the heterogeneity and complicated pathogenesis of AD,it is difficult to make reliable diagnoses using only a single blood *** review provides an overview of the progress achieved to date with the use of SCD blood biomarkers in patients with preclinical AD,highlighting the key areas of application and current challenges.

关键词： Alzheimer’s disease Biomarker Blood Evolving technologies Subjective cognitive decline

Weakening CO poisoning over size-and support-dependent Pt_(n)/X-graphene catalyst(X=C,B,N,n=1-6,13)

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Rare Metals 2023年第4期42卷 1138-1145页

作者： An-Qi Dong Hui Li Han-Ming Wu kai-Xiang Li Yuan-kai shao Zhen-Guo Li Shu-Hui Sun Wei-Chao Wang Wei-Bo Hu Department of Electronics and Tianjin Key Laboratory of Photo-Electronic Thin Film Device and Technology Nankai UniversityTianjin300071China College of Information Science and Engineering Jiaxing UniversityJiaxing314001China Zhejiang Engineering Research Center of Intelligent Human Health Situation Awareness Jiaxing UniversityJiaxing314001China National Engineering Laboratory for Mobile Source Emission Control Technology China Automotive Technology&Research Center Co.LtdTianjin300300China Institute National de la Recherche Scientifique(INRS) CentreÉnergie Matériaux et TélécommunicationsVarennesQuébecJ3X 1P7Canada College of Electronic Information and Optical Engineering Nankai UniversityTianjin300071China

CO poisoning is one of the obstacles for platinum catalysts toward the electro-catalysis process for proton exchange membrane fuel cell(PEMFC)or direct methanol fuel cell(DMFC).Herein,we aim to weaken the CO poisoning... 详细信息

关键词： sizes methanol size

“Block and attack”strategy for tumor therapy through ZnO_(2)/siRNA/NIR-mediating Zn^(2+)-overload and amplified oxidative stress

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聚集体(英文)

聚集体(英文) 2023年第4期4卷 152-165页

作者： Yuping Jiang kai shao Fenglan Zhang Tianyi Wang Lei Han Xiaoying Kong Jinsheng Shi College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural UniversityQingdaoChina School of Medicine and Pharmacy Ocean University of ChinaQingdaoChina Department of Central Laboratory Qilu Hospital(Qingdao)Cheeloo College of MedicineShandong UniversityQingdaoChina Department of Public Course Teaching University of Health and Rehabilitation SciencesQingdaoChina

Intracellular zinc ion(Zn^(2+))accumulation disrupts the Zn^(2+)homeostasis,providing an ion-overloading anticancer strategy with great *** self-adaptation of tumor cells to ion concentration,however,puts forward higher requirements for the design of ion-overloading ***,“block and attack”antitumor strategy was applied through a composite nanomaterials(UHSsPZH NPs).The strategy demonstrated powerful ion interference ability through both“blocking”the efflux of excess Zn^(2+)via gene silencing and“attacking”tumor cells via target delivery of ZnO_(2).After cellular internalization,ZnO_(2) was degraded to Zn^(2+)and hydrogen peroxide(H_(2)O_(2)),and the gene expression of zinc transporter 1(ZnT1)was silenced by targeting of released siRNA,which together caused intracellular Zn2+-*** of Zn^(2+)further interfered with intracellular Ca^(2+)homeostasis,inhibited the electron transport chain and promoted the production of endogenous reactive oxygen species(ROS),which assisted the“attack”to tumor cells together with the exogenous ROS generated by UHSsPZH NPs under 980 nm laser *** summary,this work supplies a“block and attack”strategy for the application of ion homeostasis interference in tumor therapy.

关键词： photodynamic therapy siRNA tumor therapy Zn^(2+)-overload ZnO_(2)

Twisted lattice nanocavity with theoretical quality factor exceeding 200 billion

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Fundamental Research 2023年第4期3卷 537-543页

作者： Ren-Min Ma Hong-Yi Luan Zi-Wei Zhao Wen-Zhi Mao shao-Lei Wang Yun-Hao Ouyang Zeng-kai shao State Key Laboratory for Mesoscopic Physics and Frontiers Science Center for Nano-Optoelectronics School of PhysicsPeking UniversityBeijing 100871China Collaborative Innovation Center of Quantum Matter Beijing 100871China Peking University Yangtze Delta Institute of Optoelectronics Nantong 226010JiangsuChina National Biomedical Imaging Center Peking UniversityBeijing 100871China

Simultaneous localization of light to extreme spatial and spectral scales is of high importance for testing fundamental physics and various ***,there is a longstanding trade-off between localizing a light field in space and in *** we discover a new class of twisted lattice nanocavities based on mode locking in momentum *** twisted lattice nanocavity hosts a strongly localized light field in a 0.048𝜆3 mode volume with a quality factor exceeding 2.9×1011(∼250𝜇s photon lifetime),which presents a record high figure of merit of light localization among all reported optical *** on the discovery,we have demonstrated silicon-based twisted lattice nanocavities with quality factor over 1 *** result provides a powerful platform to study light-matter interaction in extreme conditions for tests of fundamental physics and applications in nanolasing,ultrasensing,nonlinear optics,optomechanics and quantum-optical devices.

关键词： Nanocavities Twisted lattice Quality factor Mode volume Flatband Mode coupling

Enhancement of low-temperature toughness of Fe-Mn-C-Al alloy by rare earth Ce-modified inclusions

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Journal of Iron and Steel Research(International) 2024年第1期31卷 157-173页

作者： Guang-kai Yang Chang-ling Zhuang Yi-zhuang Li Chen Hu shao-bo Li College of Materials and Metallurgy Guizhou UniversityGuiyang550025GuizhouChina Department of Mechanical Engineering University of Hong KongHong Kong999077China The State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang110819LiaoningChina State Key Laboratory of Public Big Data Guizhou UniversityGuiyang550025GuizhouChina

Fe-Mn-C-Al alloys have been recognized as promising materials for certain low-temperature applications due to their exceptional mechanical properties and ***,their limited low-temperature toughness restricts their large-scale applications in specific *** influence of trace amounts of rare earth cerium(Ce)on the low-temperature toughness of Fe-18Mn-0.6C-1.8Al alloys was *** addition of Ce effectively alters the inclu-sions in the alloy,transforming large-sized irregular inclusions into fine ellipsoidal rare earth *** leads to a significant reduction in both the proportion and average size of the inclusions,resulting in their effective dispersion throughout the matrix and improved cryogenic *** presence of Ce-containing inclusions within the matrix reduces stress concentration,thereby inhibiting microcrack formation and improving impact absorption ***-cally,the addition of rare earth Ce alters the fracture behavior of the material at room temperature and low temperature,changing from brittle cleavage fracture to a more ductile failure *** impact toughness of the Fe-Mn-C-Al alloy is significantly improved by the addition of 0.0048 wt.%Ce,particularly at-196℃where the impact toughness reaches 103.6 J/cm^(2),representing an impressive improvement of 87.3%.

关键词： Fe-Mn-C-Al alloy Rare earth Low-temperature toughness Inclusion Fracture