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Screening and identification of functional bacterial attachment genes in aerobic granular sludge

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Journal of Environmental Sciences 2024年第7期141卷 205-214页

作者： kai qiao Tingting Zhao LeiWang Wei Zhang WeiMeng Fan Liu Xu Gao Jianrong Zhu School of Environment Beijing Normal UniversityBeijing 100875China State Key Laboratory of Water Simulation Beijing 100875China R&D Centre of Aerobic Granule Technology Beijing 100875China

The screening and identification of attachment genes is important to exploring the formation mechanism of biofilms at the gene *** is helpful to the development of key culture technologies for aerobic granular sludge(AGS).In this study,genome-wide sequencing and gene editing were employed for the first time to investigate the effects and functions of attachment genes in *** the help of whole-genome analysis,ten attachment genes were screened from thirteen genes,and the efficiency of gene screening was greatly ***,two attachment genes were selected as examples to further confirm the gene functions by constructing gene-knockout recombinant mutants of Stenotrophomonas maltophilia;when the two attachment genes were knocked out,the attachment potential was reduced by 50.67%and 43.93%,*** results provide a new theoretical principle and efficient method for the development of AGS from the perspective of attachment genes.

关键词： Attachment gene Genome-wide sequence Gene-knockout Attachment potential Aerobic granular sludge biofilm

Application of pulsed chemical vapor deposition on the SiO_(2)-coated TiO_(2) production within a rotary reactor at room temperature

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Chinese Journal of Chemical Engineering 2022年第5期35卷 22-31页

作者： Ke Yang Shan Zhong Hairong Yue Siyang Tang Kui Ma Changjun Liu kai qiao Bin Liang School of Chemical Engineering Sichuan UniversityChengdu 610065China Institute of New Energy and Low-carbon Technology Chengdu 610207China

Pulsed chemical vapor deposition(P-CVD)is a promising technology for the surface modification of TiO_(2) *** the scale-up application of P-CVD,a custom-designed rotary reactor and corresponding coating process at room temperature was developed in the present *** obtained SiO_(2)-coated TiO_(2) particles were characterized by various measures including high-resolution transmission electron microscope,Fourier transform infrared spectroscopy,X-ray diffraction,*** results illustrated that the SiO_(2) films with a thickness of(3.7±0.7)nm were successfully deposited onto the surface of TiO_(2) *** to the dye degradation tests and acid solubility measurement,the deposited film can effectively inhibit the photocatalytic activity and enhance the weatherability of the TiO_(2) *** potential measurements showed that the SiO_(2)-coated TiO_(2) is possible to be stably dispersed in the pH range of 6.9–*** coating process made the whiteness of TiO_(2) particles decreased slightly but still sufficient(97.3±0.1)for ***,the properties of the TiO_(2) particles coated by PCVD were compared with the particles coated by traditional wet chemical *** is shown that the P-CVD can produce thinner but denser films with better photoactivity suppression *** developed coating process within the rotary reactor was proved practically feasible and convenient for the scale-up production of SiO_(2)-coated TiO_(2) via P-CVD.

关键词： Film Pulsed-CVD Scale-up Deactivation Pigmentary properties

Influence of pore structure on thermal stress distribution inside coal particles during primary fragmentation

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Particuology 2024年第2期85卷 49-61页

作者： kai qiao Shan Zhong Siyang Tang Ke Yang Hairong Yue Kui Ma Lei Song Bin Liang School of Chemical Engineering Sichuan UniversityChengdu610065China Institute of New Energy and Low-carbon Technology Chengdu610207China

Thermal stress is an important reason of coal particle primary fragmentation,during which the role of pore structure is *** stress induced fragmentation experiments were conducted with low volatile coal/char particles,and the results show that the fragmentation severity enhances with increasing *** porous thermal stress models were developed with finite element method,and the influences of the pore shape,size,position and porosity on the thermal stress were *** maximum thermal stress inside particle increases with pore curvature,the pore position affects the thermal stress more significantly at the particle center and *** expectation of the maximum tensile thermal stress linearly increases with porosity,making the particles with higher porosity easier to fragment,contrary to the conclusion deduced from the devolatilization *** obtained results are valuable for the analysis of different thermal processes concerning the thermal stresses of the solid feedstocks.

关键词： Coal particle Primary fragmentation Thermal stress Pore structure Porosity

Local singularity and S–A methods for analyzing ore-producing anomalies in the Jianbiannongchang area of Heilongjiang,China

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Acta Geochimica 2023年第2期42卷 360-372页

作者： Zhonghai Zhao kai qiao Yiwen Liu Xiaomeng Cui Binbin Cheng Shanshan Liang Chenglu Li College of Mining Liaoning Technical UniversityFuxin 123000China Heilongjiang Institute of Natural Resources Survey Haerbin 150036China

The Heilongjiang Jianbiannongchang area is located at the confluence of the Great and Lesser Xing’an *** area has a complex magmatic and tectonic evolutionary history that has resulted in a complex and diverse geological background for *** this study,isometric logarithmic ratio(ILR)transformations of Au,Cu,Pb,Zn,and Sb contents were performed in the1:50,000 soil geochemical data of the Jianbiannongchang *** principal component analysis(RPCA)was conducted based on ILR *** local singularity and spectrum-area(S-A)methods were used to extract information on mineralogic *** results showed that:(1)the transformed data eliminated the influence of the original data closure effect,and the PC1and PC2 information obtained by applying RPCA reflected ore-producing element anomalies dominated by Au and Cu.(2)The local singularity method can enhance the information of the local strong and weak slow *** performing local singularity analysis on PC1 and PC2,the obtained local anomalies reflected the local singularity spatial anomaly patterns related to Cu and Au mineralization in this area,which is an effective method for trapping ore-producing anomalies.(3)Furthermore,the composite anomaly decomposition of PC1 and PC2 was performed using the S-A method,and the screened anomalous and background fields reflect the ore-producing anomalies related to Cu and Au *** information is in agreement with known Cu and Au mineralization.(4)The geochemical anomalies with mineralization potential were obtained outside the known mineralization sites by integrating the information of oreproducing anomalies extracted by the local singularity and S-A methods,providing the theoretical basis and exploration direction for future exploration in the study area.

关键词： Geochemistry Local singularity S-A method Robust principal component analysis Jianbiannongchang area in Heilongjiang Province

Isolation and characterization of a strain with high microbial attachment in aerobic granular sludge

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Journal of Environmental Sciences 2021年第8期33卷 194-203页

作者： Tingting Zhao kai qiao Lei Wang Wei Zhang Wei Meng Fan Liu Xu Gao Jianrong Zhu School of Environment Beijing Normal UniversityBeijing 100875China State Key Laboratory of Water Simulation Beijing 100875China R&D Centre of Aerobic Granule Technology Beijing 100875China

Aerobic granule is a special microbial aggregate associated with biofilm *** formation of aerobic granular sludge is primarily depending on its bacterial community and relevant microbiological *** this experiment,a strain with high microbial attachment was isolated from aerobic granular sludge,and the detailed characteristics were *** high attachment ability could reach 2.34(OD600 nm),while other low attachment values were only around 0.06-0.32,which indicated a big variation among the different *** strain exhibited a very special morphology with many fibric fingers under SEM observation.A distinctive behaviour was to form a spherical particle by themselves,which would be very beneficial for the formation and development of granular *** EPS measurement showed that its PN content was higher than low attachment bacteria,and 3 DEEM confirmed that there were some different *** on the 16 S rRNA analysis,it was identified to mostly belong to *** augmentation to particle sludge cultivation demonstrated that the strain could significantly promote the formation of aerobic ***,it was strongly suggested that it might be used as a good and potential model strain or chassis organism for the aerobic granular sludge formation and development.

关键词： Bacterial isolates Microbial attachment Aerobic granular sludge Biofilm 16S rRNA

Secondary organic aerosol formation from straw burning using an oxidation flow reactor

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Journal of Environmental Sciences 2022年第4期34卷 249-258页

作者： Hui Wang Song Guo Zhijun Wu kai qiao Rongzhi Tang Ying Yu Weizhao Xu Wenfei Zhu Liwu Zeng Xiaofeng Huang Lingyan He Mattias Hallquist State Key Joint Laboratory of Environmental Simulation and Pollution Control International Joint Laboratory for Regional Pollution ControlMinistry of Education(IJRC)College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China Collaborative Innovation Center of Atmospheric Environment and Equipment Technology Nanjing University of Information Science&TechnologyNanjing 10044China Key Laboratory for Urban Habitat Environmental Science and Technology College of Environment and EnergyPeking University Shenzhen Graduate SchoolShenzhen 518055China Department of Chemistry and Molecular Biology Atmospheric ScienceUniversity of GothenburgSE-41296 GothenburgSweden

Herein,we use an oxidation flow reactor,Gothenburg:Potential Aerosol Mass(Go:PAM)reactor,to investigate the secondary organic aerosol(SOA)formation from wheat straw *** burning emissions are exposed to high concentrations of hydroxyl radicals(OH)to simulate processes equivalent to atmospheric oxidation of 0-2.55 *** volatile organic compounds(VOCs)were investigated,and particles were measured before and after the Go:PAM *** influence of water content(i.e.5%and 11%)in wheat straw was also *** burning stages,the flaming stage,and non-flaming stages,were *** particle emission factors(EFs)at a water content of 11%(~3.89 g/kg-fuel)are significantly higher than those at a water content of 5%(~2.26 g/kg-fuel)during the flaming ***,the water content showed no significant influence at the non-flaming *** of aromatics at a non-flaming stage(321.8±46.2 mg/kg-fuel)are larger than that at a flaming stage(130.9±37.1 mg/kg-fuel).The OA enhancement ratios increased with the increase in OH exposure at first and decreased with the additional increment of OH *** maximum OA enhancement ratio is~12 during the non-flaming stages,which is much higher than~1.7 during the flaming *** mass spectrum of the primary wheat burning organic aerosols closely resembles that of resolved biomass burning organic aerosols(BBOA)based on measurements in ambient *** results show that large gap(0%-90%)still remains to estimate biomass burning SOA if only the oxidation of VOCs were included.

关键词： Gothenburg:Potential Aerosol Mass(Go:PAM)reactor Wheat straw burning Flaming Non-flaming Secondary organic aerosol formation

Study on cosmogenic radioactive production in germanium as a background for future rare event search experiments

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Nuclear Science and Techniques 2020年第6期31卷 22-29页

作者： Yu-Lu Yan Wei-Xin Zhong Shin-Ted Lin Jing-Jun Zhu Chen-kai qiao Lei Zhang Yu Liu Qian Yue Hao-Yang Xing Shu-Kui Liu College of Physics Sichuan UniversityChengdu 610064China School of Physics Nankai UniversityTianjin 30071China Institute of Nuclear Science and Technology Sichuan UniversityChengdu 610065China Department of Engineering Physics Tsinghua UniversityBeijing 100084China

Rare event search experiments are one of the most important topics in the field of fundamental physics,and high-purity germanium(HPGe)detectors with an ultralow radioactive background are frequently used for such ***,cosmogenic activation contaminates germanium crystals during transport and *** this study,we investigated the movable shielding containers of HPGe crystals using Geant4 and CRY Monte Carlo *** production rates of 68Ge,65Zn,60Co,55Fe,and 3H were obtained individually for different types of cosmic *** validity of the simulation was confirmed through a comparison with the available experimental *** on this simulation,we found that the interactions induced by neutrons contribute to approximately 90%of the production rate of cosmogenic *** addition,by adding an optimized shielding structure,the production rates of cosmogenic radionuclides are reduced by about one order of *** results show that it is feasible to use a shielding container to reduce the cosmogenic radioactivity produced during the transport and storage of high-purity germanium on the ground.

关键词： High-purity germanium Shielding structure Geant4 Transportation

Efficacy and Safety of Lenalidomide in the Treatment of Multiple Myeloma： A Systematic Review and Meta-analysis of Randomized Controlled Trials

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Chinese Medical Journal 2015年第9期128卷 1215-1222页

作者： Shu-kai qiao Xiao-Nan Guo Jin-Hai Ren Han-Yun Ren Department of Hematology The Second Hospital of Hebei Medical University Shijiazhuang Hebei 050000 China Department of Hematology Peking University First Hospital Beijing 100034 China

Background：Lenalidomide has emerged as an important treatment for patients with multiple myeloma （MM）.However,its role in the management of MM is still controversial and requires further *** aim of this study was to evaluate efficacy and safety of lenalidomide for MM using a ***：We searched the electronic databases including：PubMed,EMBASE and the Cochrane Center Register of Controlled *** randomized clinical trials were identified,which included a total of 2357 patients with MM who received lenalidomide-containing,noncontaining lenalidomide regimens or placebo as induction therapy or maintenance *** outcomes included overall response （OR） rate,complete response （CR） rate,3-year progression-free survival （PFS） rate,3-year overall survival （OS） rate,and different types of treatment-related adverse *** calculated the risk ratios （RRs） as well as their 95％ confidence intervals of these outcomes and pooled the results using RevMan 5.2 ***：For patients with previously untreated MM,OR rate and CR rate was significantly higher in lenalidomide-containing group than the control *** relapsed or refractory MM patients,lenalidomide-containing regimens significantly improved the OR rate,CR rate,3-year PFS rate and 3-year OS *** regard to MM patients after autologous stem cell transplantation,lenalidomide maintenance therapy significantly improved 3-year PFS rate but did not result in improved 3-year OS *** terms oftoxicities,lenalidomide therapy has a higher rate of Grade 3-4 grade cytopenias,infection,deep-vein thrombosis,and ***,the incidence of second primary malignancies was significantly higher in the lenalidomide ***：The lenalidomide-containing regimens as induction therapy clearly increased response rates and improved intervals of survival with acceptable toxicity rates for patients with ***,when physicians choose to use the lenalidomide as maintenance

关键词： Lenalidomide Meta-analysis Multiple Myeloma

基于时间相关单光子计数的穿透成像激光雷达研究(特邀)

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红外与激光工程 2022年第8期51卷 114-126页

作者：乔凯杨杰靳辰飞哈尔滨工业大学物理学院黑龙江哈尔滨150001

激光雷达是一种能够快速获取目标三维信息的光学传感器,凭借精确定位与高效识别能力,其在军事侦察、无人驾驶、空间交会对接等领域发挥着越来越重要的作用。然而在浓雾、烟尘、海水等复杂的气候或环境下,传输介质对光场的强烈散射作用... 详细信息

激光雷达是一种能够快速获取目标三维信息的光学传感器,凭借精确定位与高效识别能力,其在军事侦察、无人驾驶、空间交会对接等领域发挥着越来越重要的作用。然而在浓雾、烟尘、海水等复杂的气候或环境下,传输介质对光场的强烈散射作用会造成传统激光雷达接收信号的严重退化,致使其性能迅速降低,甚至根本无法正常使用。针对该类环境下接收信号的退化特性,首先建立了Monte Carlo的Mie散射瞬态光场传输模型,接着通过计算机模拟仿真了传输光场的时域分布规律,针对该规律研究了时域信号去散射效应的滤波算法,最后在实验室搭建了基于时间相关单光子计数的穿透成像激光雷达系统,开展了雾气模拟环境下的成像实验,结果验证了该穿透成像激光雷达系统的处理方法对目标图像重构质量的提高具有较好的效果。该研究为激光雷达在复杂散射环境中的进一步应用提供了基础。

关键词：激光雷达穿透成像 Mie散射 TCSPC