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mdbHLH3 modulates apple soluble sugar content by activating phosphofructokinase gene expression

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Journal of Integrative Plant Biology 2022年第4期64卷 884-900页

作者： Jian-Qiang Yu kai-Di Gu Li-Li Zhang Cui-Hui Sun Quan-Yan Zhang Jia-Hui Wang Chu-Kun Wang Wen-Yan Wang Meng-Chi Du Da-Gang Hu National Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai'an 271018China College of Resources and Environment Linyi UniversityLinyi 276005China MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation Tai'an 271018China Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency Tai'an 271018China

Sugars are involved in plant growth,fruit quality,and signaling ***,understanding the mechanisms involved in soluble sugar accumulation is essential to understand fruit ***,we report that md PFPβ,a pyrophosphatedependent phosphofructokinase gene,regulates soluble sugar accumulation by enhancing the photosynthetic performance and sugar-metabolizing enzyme activities in apple(Malus domestica Borkh.).Biochemical analysis revealed that a basic helix-loop-helix(b HLH)transcription factor,mdb HLH3,binds to the md PFPβpromoter and activates its expression,thus promoting soluble sugar accumulation in apple *** addition,md PFPβoverexpression in tomato influenced photosynthesis and carbon metabolism in the ***,we determined that mdb HLH3 increases photosynthetic rates and soluble sugar accumulation in apple by activating md PFPβ*** results thus shed light on the mechanism of soluble sugar accumulation in apple leaves and fruit:mdb HLH3 regulates soluble sugar accumulation by activating md PFPβgene expression and coordinating carbohydrate allocation.

关键词： apple bHLH fructose 6-phosphate fruit quality phosphofructokinase soluble sugars

小麦脱植基相关基因在春性小麦叶片叶绿素降解过程中的作用分析

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作物杂志 2022年第2期 81-88页

作者：赵锴晋秀娟孙丽丽闫荣岳卢娟郭峰 md Ashraful Islam 史雨刚孙黛珍山西农业大学农学院山西晋中030801

小麦叶片的衰老会导致产量的损失,而叶绿素降解是小麦叶片衰老的明显特征,分析小麦叶绿素降解过程中脱植基反应的相关基因叶绿素酶(TaCLH)和脱镁叶绿素水解酶(TaPPH)在春性小麦叶片衰老过程中的作用,为解析小麦叶绿素降解的分子机制提... 详细信息

小麦叶片的衰老会导致产量的损失,而叶绿素降解是小麦叶片衰老的明显特征,分析小麦叶绿素降解过程中脱植基反应的相关基因叶绿素酶(TaCLH)和脱镁叶绿素水解酶(TaPPH)在春性小麦叶片衰老过程中的作用,为解析小麦叶绿素降解的分子机制提供参考。以10个春性小麦品种为参试材料,对衰老过程中TaCLH和TaPPH的相对表达量进行测定,结合不同品种在开花后不同时期的相对叶绿素含量(SPAD)、功能绿叶面积(GLAD)和叶绿素荧光参数的变化规律,研究叶片衰老过程中TaCLH和TaPPH与SPAD、GLAD和叶绿素荧光参数的相关关系。结果表明,TaPPH的相对表达量与GLAD、SPAD及叶绿素荧光参数[ETR、Fv/Fm、Y(Ⅱ)]等生理指标之间存在极显著负相关关系,与TaCLH相对表达量存在极显著正相关关系,表明TaPPH在春小麦叶绿素降解过程的脱植基反应中起主要作用。

关键词：小麦叶绿素荧光参数 TaCLH Ta PPH 基因表达

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某推进剂用聚氨酯包覆层配方研究及性能测试

固体火箭技术 2006年第2期29卷 126-129页

作者：朱开金萧忠良中北大学化工系太原030008

利用2,4-甲苯二异氰酸酯(TD I)和液化4,4-二苯基甲烷二异氰酸酯(md I)的混合酯合成聚异氰酸酯,进而合成聚氨酯(PU)粘接剂,选择有机物联二脲作为该粘接剂的填料。以上述两者作为某推进剂包覆层的主成分,得到的包覆层较单体二异氰酸酯合成... 详细信息

利用2,4-甲苯二异氰酸酯(TD I)和液化4,4-二苯基甲烷二异氰酸酯(md I)的混合酯合成聚异氰酸酯,进而合成聚氨酯(PU)粘接剂,选择有机物联二脲作为该粘接剂的填料。以上述两者作为某推进剂包覆层的主成分,得到的包覆层较单体二异氰酸酯合成的PU粘接剂和无机填料得到的包覆层具有良好的力学性能、相容性、防NG迁移性和阻燃性等。研究结果表明,选择TD I和液化md I的比例为7∶3,联二脲加入量为40%较为适宜。同时,对力学性能、相容性和阻燃性改善的原因也进行了探讨。

关键词： 2,4-甲苯二异氰酸酯液化4,4-二苯基甲烷二异氰酸酯聚氨酯包覆层

White Matter Changes in Posttraumatic Stress Disorder Following Mild Traumatic Brain Injury： A Prospective Longitudinal Diffusion Tensor Imaging Study

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Chinese Medical Journal 2016年第9期129卷 1091-1099页

作者： Li Li Gang Sun kai Liu Min Li BO Li Shao-Wen Qian Li-Li Yu Institute of Postgraduates The Second Military Medical University Shanghai 200032 China Department of Medical Imaging Jinan Military General Hospital Jinan Shandong 250031 China Department of Statistics~ Ji'nan Military General Hospitail Jinan Sh'andong 2500311 China

Background： The ability to predict posttraumatic stress disorder （PTSD） is a critical issue in the management of patients with mild traumatic brain injury （mTBI）, as early medical and rehabilitative interventions may reduce the risks of long-term cognitive changes. The aim of the present study was to investigate how diffusion tensor imaging （DTI） metrics changed in the transition from acute to chronic phases in patients with mTBI and whether the alteration relates to the development of PTSD. Methods： Forty-three patients with mTBI and 22 healthy volunteers were investigated. The patients were divided into two groups： successful recovery （SR, n = 22） and poor recovery （PR, n = 21）, based on neurocognitive evaluation at 1 or 6 months after injury. All patients underwent magnetic resonance imaging investigation at acute （within 3 days）, subacute （10-20 days）, and chronic （1-6 months） phases after injury. Group differences of fractional anisotropy （FA） and mean diffusivity （md） were analyzed using tract-based spatial statistics （TBSS）. The accuracy of DTI metrics for classifying PTSD was estimated using Bayesian discrimination analysis. Results： TBSS showed white matter （WM） abnormalities in various brain regions. In the acute phase, FA values were higher for PR and SR patients than controls （all P 〈 0.05）. In subacute phase, PR patients have higher mean md than SR and controls （all P 〈 0.05）. In the chronic phase, lower FA and higher md were observed in PR compared with both SR and control groups （all P 〈 0.05）. PR and SR groups could be discriminated with a sensitivity of 73%, specificity of 78%, and accuracy of 75.56%, in terms of md value in subacute phase. Conclusions： Patients with mTBI have multiple abnormalities in various WM regions. DTI metrics change over time and provide a potential indicator at subacute stage for PTSD following mTBI.

关键词： Diffusion Tensor Imaging Mild Traumatic Brain Injury Posttraumatic Stress Disorder Tract-based Spatial Statistics

A long-term Global LAnd Surface Satellite(GLASS)data-set for environmental studies

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International Journal of Digital Earth 2013年第S01期6卷 5-33页

作者： Shunlin Liang Xiang Zhao Suhong Liu Wenping Yuan Xiao Cheng Zhiqiang Xiao Xiaotong Zhang Qiang Liu Jie Cheng Hairong Tang Yonghua Qu Yancheng Bo Ying Qu Huazhong Ren kai Yu John Townshend The State Key Laboratory of Remote Sensing Science Jointly Sponsored by Beijing Normal University and Institute of Remote Sensing Applications of Chinese Academy of SciencesBeijingChina College of Global Change and Earth System Sciences Beijing Normal UniversityBeijingChina Department of Geographical Sciences University of MarylandMDUSA School of Geography Beijing Normal UniversityBeijingChina Institute of Electronics Chinese Academy of SciencesBeijingChina

Recently,five Global LAnd Surface Satellite(GLASS)products have been released:leaf area index(LAI),shortwave broadband albedo,longwave broadband emissivity,incident short radiation,and photosynthetically active radiation(PAR).The first three products cover the years 19822012(LAI)and 19812010(albedo and emissivity)at 15 km and 8-day resolutions,and the last two radiation products span the period 20082010 at 5 km and 3-h *** products have been evaluated and validated,and the preliminary results indicate that they are of higher quality and accuracy than the existing *** particular,the first three products have much longer time series,and are therefore highly suitable for various environmental *** paper outlines the algorithms,product characteristics,preliminary validation results,potential applications and some examples of initial analysis of these products.

关键词： earth observation global environmental change remote sensing

青海血蜱重组蛋白Hq001的结构预测与原核表达

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中国媒介生物学及控制杂志 2024年第2期35卷 138-144页

作者：刘岳青马林源马静陈开廷曹美娜王雪薇王鹏高金亮内蒙古科技大学包头医学院内蒙古包头014000 鄂尔多斯市中心医院分子医学实验室内蒙古鄂尔多斯017000 内蒙古科技大学包头医学院第一附属医院医学检验科内蒙古包头014000 内蒙古医科大学鄂尔多斯临床医学院内蒙古鄂尔多斯017000 河南中医药大学第三附属医院河南郑州450003

目的对从青海血蜱唾液腺cDNA文库中克隆出的1个新基因Hq001编码的蛋白质进行三维理论模型预测,并评估其与Kunitz型蜱源抗凝血蛋白质之间的关系。方法构建重组质粒pET-30a-Hq001并转化至感受态大肠埃希菌(Escherichia coli)BL21(DE3)中,... 详细信息

目的对从青海血蜱唾液腺cDNA文库中克隆出的1个新基因Hq001编码的蛋白质进行三维理论模型预测,并评估其与Kunitz型蜱源抗凝血蛋白质之间的关系。方法构建重组质粒pET-30a-Hq001并转化至感受态大肠埃希菌(Escherichia coli)BL21(DE3)中,对目的蛋白进行重组表达。使用开源软件AlphaFold v2对去除信号肽的Hq001氨基酸序列进行三级结构预测,以此得到三维理论模型。并使用开源软件GROMACS v2023对三维理论模型进行分子动力学(md)模拟优化,应用ANOLEA和MolProbity等工具对模型质量进行评价。结果由Hq001构建的重组表达质粒pET30a-Hq001在*** BL21中表达后以包涵体形式存在。序列比对与结构建模结果均表明Hq001蛋白具有典型双Kunitz-BPTI型结构域,并与Bikunin、Boophilin、Ornithodorin、Ixolaris等具有抗凝血活性的KunitzBPTI型蛋白具有序列及结构相似性。经过1 ns的分子动力学模拟优化后理论模型均方根偏差在3?左右达到平衡,选取其中能量态最低的结构模型(总能量=-20716640 kJ/mol)作为最终模型。结论蛋白质三维理论模型证实Hq001蛋白具有保守的Kunitz-BPTI型结构域,与来自微小扇头蜱的Boophilin在序列与结构上均较为相似,Hq001蛋白可能具有Kunitz型丝氨酸蛋白酶抑制剂相似的结构与功能。

关键词：青海血蜱 Kunitz-BPTI AlphaFold 格罗宁根化学模拟体系分子模拟分子动力学

Photocurrent Studies of Ultraviolet Detector Based on Gallium Nitride Epilayer

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Chinese Physics Letters 1996年第11期13卷 874-877页

作者： YANG kai QIN Lin-hong ZHANG Rong SHEN Bo ZHENG You-dou HUANG Zhen-chun JCChen Department of Physics Nanjing UniversityNanjing 210093 Department of Electrical Engineering University of Maryland Baltimore CountyBaltimoreMD 21228-5398USA

The properties of photoconductive blue and ultraviolet detector based on single crystal GaN epilayer grown on sapphire substrate by metalorganic chemical vapor deposition were *** observed optical absorption edge of 3.39eV in optical transmission has been confirmed by the peak of 3.400eV in *** detectable energy span of the GaN device up to ultraviolet was obtained by photocurrent *** dependence of the intensity of photocurrent on the chopper frequency in the measurement was studied,and an easy method was used to determine the response time as 0.4s.

关键词： measurement sapphire optical

Mako:A Graph-based Pattern Growth Approach to Detect Complex Structural Variants

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 205-218页

作者： Jiadong Lin Xiaofei Yang Walter Kosters Tun Xu Yanyan Jia Songbo Wang Qihui Zhu Mallory Ryan Li Guo Chengsheng Zhang The Human Genome Structural Variation Consortium Charles Lee Scott E.Devine Evan E.Eichler kai Ye School of Automation Science and Engineering Faculty of Electronic and Information EngineeringXi’an Jiaotong UniversityXi’an 710049China MOE Key Lab for Intelligent Networks&Networks Security Faculty of Electronic and Information EngineeringXi’an Jiaotong UniversityXi’an 710049China Genome Institute the First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061China Leiden Institute of Advanced Computer Science Faculty of ScienceLeiden UniversityLeiden 2311EZNetherland School of Computer Science and Technology Faculty of Electronic and Information EngineeringXi’an Jiaotong UniversityXi’an 710049China The Jackson Laboratory for Genomic Medicine FarmingtonCT 06032USA Precision Medicine Center the First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061China Institute for Genome Sciences University of Maryland School of MedicineBaltimoreMD 21201USA Department of Genome Sciences University of Washington School of MedicineSeattleWA 98119USA Howard Hughes Medical Institute University of WashingtonSeattleWA 98195USA The School of Life Science and Technology Xi’an Jiaotong UniversityXi’an 710049China 不详

Complex structural variants(CSVs) are genomic alterations that have more than two breakpoints and are considered as the simultaneous occurrence of simple structural ***,detecting the compounded mutational signals of CSVs is challenging through a commonly used model-match *** a result,there has been limited progress for CSV discovery compared with simple structural ***,we systematically analyzed the multi-breakpoint connection feature of CSVs,and proposed Mako,utilizing a bottom-up guided model-free strategy,to detect CSVs from paired-end short-read ***,we implemented a graph-based pattern growth approach,where the graph depicts potential breakpoint connections,and pattern growth enables CSV detection without pre-defined *** evaluations on both simulated and real datasets revealed that Mako outperformed other ***,validation rates of CSVs on real data based on experimental and computational validations as well as manual inspections are around 70%,where the medians of experimental and computational breakpoint shift are 13 bp and 26 bp,***,the Mako CSV subgraph effectively characterized the breakpoint connections of a CSV event and uncovered a total of 15 CSV types,including two novel types of adjacent segment swap and tandem dispersed *** analysis of these CSVs also revealed the impact of sequence homology on the formation of *** is publicly available at https://***/xjtu-omics/Mako.

关键词： Next-generation sequencing Complex structural variant Pattern growth Graph mining Formation mechanism

苹果霉心病光谱在线检测的摆放姿态及建模方法优化研究

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光谱学与光谱分析 2021年第11期41卷 3405-3410页

作者：秦楷陈刚张剑一傅霞萍浙江理工大学机械与自动控制学院浙江杭州310018 德菲洛(杭州)科技有限公司浙江杭州310014

苹果营养丰富、口味酸甜,是深受大众喜爱的一种水果。苹果霉心病是一种真菌侵染果实病害,隐蔽性极强,一般在近成熟期果实内部发生霉变,肉眼从外观观察难以分辨,市面上大多数品种的苹果都受其影响。霉心病病果重量变轻、口感变差,严重的... 详细信息

苹果营养丰富、口味酸甜,是深受大众喜爱的一种水果。苹果霉心病是一种真菌侵染果实病害,隐蔽性极强,一般在近成熟期果实内部发生霉变,肉眼从外观观察难以分辨,市面上大多数品种的苹果都受其影响。霉心病病果重量变轻、口感变差,严重的甚至不能食用,对经济效益的影响巨大。采用可见近红外光谱分析技术,使用微型光谱仪在线无损检测苹果霉心病,针对4种苹果在线输送时摆放姿态(竖放柄朝上、竖放柄朝下、横放柄朝输送方向和横放柄垂直输送方向)的判别效果进行了优化分析。首先使用主成分分析对600~900 nm波段的透射光谱提取主成分后分别建立线性判别分析(LDA)、马氏距离(md)和K近邻法(KNN)模型并对校正集和预测集的判别准确率进行对比;其次对600~900 nm波段中心化预处理后建立偏最小二乘判别分析(PLS-DA)模型并给出4种摆放姿态的判别效果;最后使用两种机器学习算法极限学习机(ELM)和支持向量机(SVM)分别建立霉心病判别模型进行预测。对比上述所有6种判别模型,通过观察4种摆放姿态整体的判别效果得到最佳的建模方法为PLS-DA,其中竖放柄朝上和竖放柄朝下摆放的判别准确率都为93.75%,其他2种摆放姿态的判别准确率也都超过85%,再根据PLS-DA模型波段变量投影重要性指标得分值分布提取特征波段690~720 nm重新建立模型,对比4种摆放姿态效果最好的是竖放柄朝上摆放,其预测集的判别准确率达到93.75%,并且对病果的判别效果最佳。研究结果表明PLS-DA可以作为判别苹果霉心病一种有效方法,竖放柄朝上摆放可以作为苹果霉心病在线检测时一种有效姿态。

关键词：苹果霉心病光谱摆放姿态建模方法