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沉积物解磷菌的研究进展:分布、解磷能力及功能基因

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农业资源与环境学报 2023年第2期40卷 280-290页

作者：马凯王效昌谢嘉慧高丽烟台大学海洋学院山东烟台264005

磷是大多数水体富营养化和有害藻华暴发的重要诱因。当外源污染得到有效控制后,沉积物内源释放可在较长时间内成为水体磷的主要来源。作为水圈磷地球化学循环的主要驱动者,解磷菌在沉积物磷的释放过程中发挥着重要作用。然而相较于农业... 详细信息

磷是大多数水体富营养化和有害藻华暴发的重要诱因。当外源污染得到有效控制后,沉积物内源释放可在较长时间内成为水体磷的主要来源。作为水圈磷地球化学循环的主要驱动者,解磷菌在沉积物磷的释放过程中发挥着重要作用。然而相较于农业土壤而言,沉积物解磷菌的研究起步较晚,其分子机制的研究仍处于起始阶段。鉴于此,本文概括了解磷菌的主要种属、不同生境沉积物中分布的特征以及藻华生消对解磷微生物群落组成的影响,梳理了解磷菌的矿化、溶解等主要解磷机制和关键功能基因,并对水体生态系统解磷菌的未来研究方向进行了展望,旨在为藻华期间水体磷循环以及富营养化机制的研究提供新的思路。

关键词：沉积物解磷菌解磷机制功能基因有害藻华

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微波消融治疗甲状腺良性结节疗效及安全性Meta分析

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中华内分泌外科杂志 2019年第2期13卷 127-134页

作者：吴兆书韩玮鲁凯南京中医药大学附属南京中医院 210000

目的运用Meta分析系统评价微波消融治疗甲状腺良性结节的疗效及安全性。方法检索数据库Pubmed(Medline)、CochraneLibrary、Embase、中国知网(CNKI)、万方数据库、生物医学文献数据库(Sinomed)、维普数据库(VIP)中关于微波消融治疗甲状... 详细信息

目的运用Meta分析系统评价微波消融治疗甲状腺良性结节的疗效及安全性。方法检索数据库Pubmed(Medline)、CochraneLibrary、Embase、中国知网(CNKI)、万方数据库、生物医学文献数据库(Sinomed)、维普数据库(VIP)中关于微波消融治疗甲状腺良性结节的随机对照研究,按照纳入及排除标准筛选文献,利用Revman 5.3软件进行数据分析。结果最终纳入12篇文献,共1 221例患者。通过分析得到微波消融手术在以下几方面优于传统手术:手术时间[md=-50.78,95% CI=(-58.20,-43.36),Pmd=-23.21,95% CI=(-27.18,-19.23),Pmd=-3.39,95% CI=(-4.08,-2.71),Pmd=0.20,95% CI=(0.13,0.32),P<0.01,I2=0%],均得到P<0.01。结论微波消融具有手术创伤小、美观度高、恢复快、对甲状腺功能影响小,手术并发症少等优点,临床应用安全可靠。

关键词：微波消融甲状腺良性结节 Meta分析

神经生长因子治疗非动脉炎性前部缺血性视神经病变的临床效果

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中华眼视光学与视觉科学杂志 2020年第10期22卷 781-785页

作者：陈凯周洪伟江苏省淮安市涟水县人民医院眼科 223400

目的:观察神经生长因子(NGF)治疗非动脉炎性前部缺血性视神经病变(NA-AION)的临床疗效。方法:前瞻性随机对照研究。选择2016年7月至2019年6月涟水县人民医院眼科收治的NA-AION患者58例(58眼),根据随机数字表法分为对照组和观察组,对照... 详细信息

目的:观察神经生长因子(NGF)治疗非动脉炎性前部缺血性视神经病变(NA-AION)的临床疗效。方法:前瞻性随机对照研究。选择2016年7月至2019年6月涟水县人民医院眼科收治的NA-AION患者58例(58眼),根据随机数字表法分为对照组和观察组,对照组29例(29眼)予以糖皮质激素+活血通络颗粒+复方樟柳碱治疗,观察组29例(29眼)在对照组基础上加NGF治疗。比较2组治疗的总有效率、治疗前后最佳矫正视力(BCVA)、30°范围视野检查平均缺损(md)、视盘视网膜神经纤维层(RNFL)厚度。数据采用Fisher精确检验、t检验及非参数检验。结果:治疗后,观察组总有效率明显高于对照组(P=0.04),观察组BCVA小数视力0.1以上所占百分比明显高于对照组(Pmd和RNFL厚度均明显低于对照组(t=2.59,P=0.01;t=4.86,P<0.001)。2组治疗后均无明显不良反应。结论:加用NGF治疗NA-AION可获得更好的疗效,能提高患者视力,改善视野,降低RNFL厚度。

关键词：神经生长因子非动脉炎性前部缺血性视神经病变最佳矫正视力平均缺损视网膜神经纤维层

无机解磷菌对天鹅湖瀉湖沉积物内源磷释放的影响

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农业资源与环境学报 2023年第1期40卷 76-85页

作者：王效昌马凯谢嘉慧高丽烟台大学海洋学院山东烟台264005

为研究解磷菌对荣成天鹅湖瀉湖沉积物内源磷释放的影响,以前期筛选的3株无机解磷菌和表层沉积物为试验材料进行室内模拟试验,分析了不同解磷菌接种条件下水体总磷及pH、Eh等参数的动态变化,比较了试验前后沉积物各形态无机磷的含量差异... 详细信息

为研究解磷菌对荣成天鹅湖瀉湖沉积物内源磷释放的影响,以前期筛选的3株无机解磷菌和表层沉积物为试验材料进行室内模拟试验,分析了不同解磷菌接种条件下水体总磷及pH、Eh等参数的动态变化,比较了试验前后沉积物各形态无机磷的含量差异。结果表明,在试验前期,各解磷菌处理水体的pH均不同程度下降,其中沉积物灭菌组和非灭菌组分别降至6.46~6.88和6.66~6.78。试验期间各处理水体Eh不断下降,结束时沉积物-水界面呈厌氧状态。灭菌条件下解磷菌的接种明显促进了沉积物磷的释放,试验后期各加菌处理水体的总磷含量远高于对照;未灭菌条件下水体总磷上升较慢且总体水平较低,不同解磷菌处理间差异较小。试验结束时,大部分加菌处理沉积物中HCl-P(钙结合态磷)含量下降了19.92~50.08 mg·kg^(-1),BD-P(可还原态磷)含量下降1.81~4.19 mg·kg^(-1),而NaOH-P(铁铝结合态磷)未下降。研究表明,无机解磷菌的接种可明显降低上覆水pH,从而使得沉积物中HCl-P溶解释放;HCl-P为天鹅湖沉积物磷的主要赋存形态,在解磷菌作用下其对上覆水体磷水平的潜在贡献不容忽视。

关键词：沉积物无机解磷菌磷释放磷形态荣成天鹅湖

Extralevator abdominoperineal excision versus abdominoperineal excision for low rectal cancer: a meta-analysis

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Chinese Medical Journal 2019年第20期132卷 2446-2456页

作者： Xin-Yu Qi Ming Cui Mao-Xing Liu kai Xu Fei Tan Zhen-Dan Yao Nan Zhang Hong Yang Cheng-Hai Zhang Jia-Di Xing Xiang-Qian Su Peking University Health Science Center Beijing 100038China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education) Department of Gastrointestinal Surgery IVPeking University Cancer Hospital and InstituteBeijing 100142China

Background:Extralevator abdominoperineal excision (ELAPE) has become a popular procedure for low rectal cancer as compared with abdominoperineal excision (APE). No definitive answer has been achieved whether one is superior to the other. This study aimed to evaluate the safety and efficacy of ELAPE for low rectal cancer with ***:The Web of Science, Cochrane Library, Embase, and PubMed databases before September 2019 were comprehensively searched to retrieve comparative trials of ELAPE and APE for low rectal cancer. Pooled analyses of the perioperative variables, surgical complications, and oncological variables were performed. Odds ratio (OR) and mean differences (md) from each trial were pooled using random or fixed effects model depending on the heterogeneity of the included studies. A subgroup analysis or a sensitivity analysis was conducted to explore the potential source of heterogeneity when ***:This meta-analysis included 17 studies with 4049 patients, of whom 2248 (55.5%) underwent ELAPE and 1801 (44.5%) underwent APE. There were no statistical differences regarding the circumferential resection margin positivity (13.0% vs. 16.2%, OR = 0.69, 95% CI = 0.42-1.14, P = 0.15) and post-operative perineal wound complication rate (28.9% vs. 24.1%, OR = 1.21, 95% CI = 0.75-1.94, P= 0.43). The ELAPE was associated with lower rate of intraoperative perforation (6.6% vs. 11.3%, OR = 0.50, 95% CI = 0.39-0.64, P < 0.001) and local recurrence (8.8% vs. 20.5%, OR = 0.29, 95% CI = 0.21-0.41, P < 0.001) when compared with ***:The ELAPE was associated with a reduction in the rate of intra-operative perforation and local recurrence, without any increase in the circumferential resection margin positivity and post-operative perineal wound complication rate when compared with APE in the surgical treatment of low rectal cancer.

关键词： Extralevator abdominoperineal excision Abdominoperineal excision Low rectal cancer Surgical complications

Load-balancing data distribution in publish/subscribe mode

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Journal of Southeast University(English Edition) 2014年第4期30卷 428-433页

作者：李凯汪芸殷奕袁飞飞东南大学计算机科学与工程学院南京211189 东南大学教育部计算机网络与信息集成重点实验室南京211189 南京师范大学计算机与技术学院南京210046

To improve data distribution efficiency a load-balancing data distribution LBDD method is proposed in publish/subscribe *** the LBDD method subscribers are involved in distribution tasks and data transfers while receiving data themselves.A dissemination tree is constructed among the subscribers based on md5 where the publisher acts as the root. The proposed method provides bucket construction target selection and path updates furthermore the property of one-way dissemination is *** the average out-going degree of a node is 2 is guaranteed with the proposed *** experiments on data distribution delay data distribution rate and load distribution are conducted. Experimental results show that the LBDD method aids in shaping the task load between the publisher and subscribers and outperforms the point-to-point approach.

关键词： data distribution publish/subscribe mode load balance dissemination tree

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运营支撑系统的系统管理的设计与实现

计算机工程与设计 2006年第20期27卷 3881-3883页

作者：曾令华欧阳开翠温州大学计算机科学与工程学院浙江温州325027

随着社会的发展和技术的进步,国内电信企业对运营支撑系统(OSS)的依赖性越来越强,要求也越来越高。首先介绍了基于J2EE平台的OSS的基本概念,并阐述了光纤到户实现方式EPON技术的基本原理,然后设计并实现了OSS中的系统管理功能模块,其独... 详细信息

随着社会的发展和技术的进步,国内电信企业对运营支撑系统(OSS)的依赖性越来越强,要求也越来越高。首先介绍了基于J2EE平台的OSS的基本概念,并阐述了光纤到户实现方式EPON技术的基本原理,然后设计并实现了OSS中的系统管理功能模块,其独立于业务实现模块,是所有业务实现模块进行合理的组织管理、保证整个软件系统正常运行的关键。

关键词： OSS 光纤到户以太无源光网络系统管理 md5

Transcutaneous electroacupuncture alleviates postoperative ileus after gastrectomy: A randomized clinical trial

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World Journal of Gastrointestinal Surgery 2018年第2期10卷 13-20页

作者： kai-Bo Chen Yi-Qiao Lu Jian-De Chen Di-Ke Shi Zhi-Hui Huang Yi-Xiong Zheng Xiao-Li Jin Zhe-Fang Wang Wei-Dong Zhang Yi Huang Zhi-Wei Wu Guo-Ping Zhang Hang Zhang Ying-Hao Jiang Li Chen Department of General Surgery Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina Department of Obstetrics and Gynecology Women’s HospitalZhejiang University School of MedicineHangzhou 310006Zhejiang ProvinceChina Division of Gastroenterology and Hepatology Johns Hopkins University School of MedicineBaltimoreMD 21224United States Department of Gastroenterology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016Zhejiang ProvinceChina Department of General Visceral and Cancer SurgeryUniversity of CologneCologne 50937Germany Department of Thyroid Surgery Ningbo No.2 HospitalNingbo 315000Zhejiang ProvinceChina Department of Chinese Medicine Rehabilitation Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina Department of General Surgery First People’s Hospital of WenlingTaizhou 317500Zhejiang ProvinceChina

AIM To investigate the efficacy and safety of transcutaneouselectroacupuncture(TEA) to alleviate postoperative ileus(POI) after *** From April 2014 to February 2017, 63 gastric cancer patients were recruited from the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China. After gastrectomy, the patients were randomly allocated to the TEA(n = 33) or control(n = 30) group. The patients in the TEA group received 1 h TEA on Neiguan(ST36) and Zusanli(PC6) twice daily in the morning and afternoon until they passed flatus. The main outcomes were hours to the first flatus or bowel movement, time to nasogastric tube removal, time to liquid and semi-liquid diet, and hospital stay. The secondary outcomes included postoperative symptom assessment and *** Time to first flatus in the TEA group was significantly shorter than in the control group(73.19 ± 15.61 vs 82.82 ± 20.25 h, P = 0.038), especially for open gastrectomy(76.53 ± 14.29 vs 87.23 ± 20.75 h, P = 0.048). Bowel sounds on day 2 in the TEA group were significantly greater than in the control group(2.30 ± 2.61/min vs 1.05 ± 1.26/min, P = 0.017). Time to nasogastric tube removal in the TEA group was earlier than in the control group(4.22 ± 1.01 vs 4.97 ± 1.67 d, P = 0.049), as well as the time to liquid diet(5.0 ± 1.34 vs 5.83 ± 2.10 d, P = 0.039). Hospital stay in the TEA group was significantly shorter than in the control group(8.06 ± 1.75 vs 9.40 ± 3.09 d, P = 0.041). No significant differences in postoperative symptom assessment and complications were found between the groups. There were no severe adverse events related to *** TEA accelerated bowel movements and alleviated POI after open gastrectomy and shortened hospital stay.

关键词： Transcutaneous electroacupuncture Gastrectomy Postoperative ileus

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基于SWOT分析的生态视域下旅游产品设计

包装工程 2020年第4期41卷 116-121页

作者：孟凯宁郭娟龄西华大学

目的基于生态观,对现有的旅游产品外观形态设计进行探索研究。方法使用SWOT分析法对旅游产品进行剖析,为旅游产品设计提出方法和思路,从生态旅游产品的功能和外观设计两方面进行方法研究,探讨两者逻辑关系,并通过案例分析法进行验证,吸... 详细信息

目的基于生态观,对现有的旅游产品外观形态设计进行探索研究。方法使用SWOT分析法对旅游产品进行剖析,为旅游产品设计提出方法和思路,从生态旅游产品的功能和外观设计两方面进行方法研究,探讨两者逻辑关系,并通过案例分析法进行验证,吸引客源、打造生态旅游产品。结论提出生态观下旅游产品外观形态设计具体方法,从挖掘旅游地方特色、开发特色旅游产品、借鉴国内外经验等方式来促进生态旅游产品的开发。通过对生态旅游产品方法的研究,突出旅游产品的特点,提升竞争力,为旅游产品外观设计的发展提供借鉴的依据与参考,提升个人正确生态意识,促进旅游经济更好发展。

关键词： SWOT分析生态旅游产品设计