检索结果-南通市图书馆

c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1

World Journal of Gastroenterology 2008年第41期14卷 6339-6346页

作者： Meryem Güller kahina toualbi-abed Agnès Legrand Laurence Michel Alain Mauviel Dominique Bernuau Fanny Daniel Institut National de laSanté et de la Recherche Médicale U697 Hpital Saint-Louis Institut National de la Santé et de la RechercheMédicale U591 Faculté de Médecine Necker Institut National de la Santé et de la RechercheMédicale U773 Centre de Recherche Bichat Beaujon CRB3and Université Paris 7 Denis Diderot site Bichat

AIM： To investigate the effect of stable c-Fos over- expression on immortalized human hepatocyte （IHH） proliferation. METHODS： IHHs stably transfected with c-Fos （IHH- Fos） or an empty vector （IHH-C） were grown in me- dium supplemented with 1% serum or stimulated with 10% serum. Cell proliferation was assessed by cell counts, 3H-thymidine uptake and flow cytometry analyses. The levels of cell cycle regulatory proteins （Cyclin DI, E, A） cyclin dependent kinases （cdk） cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, total and phosphorylated GSK-3β and epidermal growth factor receptor （EGF-R） were assayed by Western blotting. Analysis of O/c/in D1 mRNA levels was performed by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction （PCR） analysis. Stability of Cyclin DI was studied by cycloheximide blockade experiments. RESULTS： Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorpora- tion following serum addition. Cell cycle analysis by flow cytometry showed that c-Fos accelerated the cell cycle kinetics. Following serum stimulation, Cyclin D1 was more abundantly expressed in c-Fos overexpress- ing cells. Cyclin D1 accumulation did not result from increased transcriptional activation, but from nuclear stabilization. Overexpression of c-Fos correlated with higher nuclear levels of inactive phosphorylated GSK- 3β, a kinase involved in Cyclin D1 degradation and higher levels of EGF-R mRNA, and EGF-R protein com- pared to IHH-C both in serum starved, and in serum stimulated cells. Abrogation of EGF-R signalling in IHH- Fos by treatment with AG1478, a specific EGF-R tyro- sine kinase inhibitor, prevented the phosphorylation of GSK-3β induced by serum stimulation and decreased Cyclin D1 stability in the nucleus. CONCLUSION： Our results clearly indicate a positive role for c-Fos in cell cycle regulation in hepatocytes. Importantly, we delineate

关键词： c-Fos Cyclin DI GSK-3 Cell growth, Cell cycle Hepatoma Epidermal growth factor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Effects of Gamma Irradiation on Molecular Weight, Morphology and Physical Properties of PHBV/Cloisite 30B Bionanocomposites

引用

Journal of Renewable Materials 2019年第9期7卷 807-820页

作者： kahina Iggui Mustapha Kaci Mohamed Mahlous Nicolas Le Moigne Anne Bergeret Laboratoire des Matériaux Polymères Avancés(LMPA) Universitéde Bejaia06000 BejaiaAlgeria Institut de Technologie UniversitéAkli Mohand OulhadjBouira 10000Algeria Centre de Recherche Nucléaire d'Alger(CRNA) Boulevard Frantz FanonAlger 16000Algeria Centre des Matériaux des Mines d’Alès(C2MA)a IMT Mines AlèsUniversitéde Montpellier6 avenue de Clavières30319 Alès CedexFrance.

In this paper,the effects of gamma irradiation on Cast poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)and PHBV/Cloisite 30B(C30B)(3 wt%)bionanocomposite prepared by melt compounding,were evaluated at various doses,i.e.,5,15,20,50 and 100 kGy at room temperature in *** in molecular weight,morphology and physical properties were *** study showed that the main degradation mechanism occurring in gamma irradiation in both Cast PHBV and C-PHBV/3C30B bionanocomposite is chain scission,responsible for the decrease of molecular *** scanning calorimetry(DSC)data indicated a regular decrease in crystallization temperature,melting temperature and crystallinity index for all irradiated samples with increasing the ***,DSC thermograms of both Cast PHBV and PHBV bionanocomposite exhibited double melting peaks due probably to changes in the PHBV crystal *** and DMA data showed a reduction in Young’s modulus,strength,elongation at break and storage modulus with the radiation dose;the decrease was however more pronounced for Cast *** morphological damages were much less pronounced for the PHBV bionanocomposite sample compared to Cast PHBV,for which some irregularities and defects were observed at 100 *** study highlighted the ability of C30B to counterbalance the detrimental effect of radiolytic degradation on the functional properties of PHBV up to 100 kGy,thus acting as a potential anti-rad.

关键词： Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV) bionano-composite cloisite 30B gamma irradiation degradation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Effects of Accelerated Photooxidation on Molecular Weight and Thermal and Mechanical Properties of PHBV/Cloisite 30B Bionanocomposites

引用

Journal of Renewable Materials 2018年第3期6卷 288-298页

作者： kahina Iggui Mustapha Kaci Nicolas Le Moigne Anne Bergeret Centre des Matériaux des Mines d’Alès(C2MA) IMT Mines AlesUniversitéde Montpellier30100 AlèsFrance Laboratory of Advanced Polymer Materials University of BéjaïaBéjaïa Province06000Algeria Institut de Technologie UniversitéAkli Mohand OulhadjBouira 10000Algeria

The effects of accelerated photooxidation on the molecular weight and thermal and mechanical properties of Cast PHBV and PHBV/Cloisite 30B(3 wt%)bionanocomposites are investigated *** size exclusion chromatography(SEC)analysis,a significant decrease in both weight and number average molecular weights was observed for all irradiated samples over time,resulting from the chain scission *** scanning calorimetry(DSC)data indicated a decrease in degree of crystallinity and melting temperature after UV exposure,with the appearance of double melting peaks related to the changes in the crystal structure of *** stability,tensile and thermo-mechanical properties were also reduced consecutively in photooxidation,being more pronounced for Cast *** study shows that the incorporation of Cloisite 30B in PHBV provides a better resistance to photooxidation in comparison with the neat polymer.

关键词： Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) organo-modified montmorillonite bionanocomposites accelerated photooxidation and degradation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antihydatic and immunomodulatory effects of Algerian propolis ethanolic extract:In vitro and in vivo study

引用

Asian Pacific Journal of Tropical Medicine 2019年第3期12卷 106-116页

作者： Nahla Deghbar Dalila Mezioug Touri kahina Yacine-Miloud Medjdoub Chafia Touil-Boukoffa Cytokines and NO Synthases-Immunity and Pathogeny team Laboratory of Cellular and Molecular Biology Faculty of Biological Sciences University of Sciences and Technology Houari Boumediene Department of Thoracic Surgery Mustapha Pacha Hospital

Objective: To evaluate the in vitro and in vivo effect of the Algerian propolis ethanolic extract(EEP) against Echinococcus granulosus(E. granulosus) infection. Methods: In vitro scolicidal activity of EEP was investigated on the protoscolices of hydatid cyst. This in vitro study was conducted by using an in vivo assay. BALB/c mice were inoculated with E. granulosus and treated with propolis for three months. Hydatid cysts development was assessed. Nitric oxide(NO), tumor necrosis factor alpha(TNF-α) production and inducible NO synthase, NF-κB, and TNF-α spleen expression were estimated by Griess method and immunofluorescence ***: Our study revealed that EEP has a high scolicidal activity against E. granulosus. Oral administration of EEP decreased TNF-α, NF-κB and inducible NO synthase expression in the spleen tissues in the CE+EEP group, in comparison with the CE group. Concomitantly, EEP treatment caused an important systemic decrease in NO and TNF-α levels. These findings are associated with the reduction of CE development. Conclusions: This is the first report demonstrating with interest the antihydatic and immunomodulatory effects of the Algerian EEP, suggesting its therapeutic potential for the hydatid disease treatment.

关键词： Cystic echinococcosis Algerian propolis Antihydatic effect Immunomodulatory effect

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Localized magnetization reversal processes in cobalt nanorods with different aspect ratios

引用

Nano Research 2015年第7期8卷 2231-2241页

作者： Marc Pousthomis Evangelia Anagnostopoulou Ioannis Panagiotopoulos Rym Boubekri Weiqing Fang Frederic Ott kahina Ait Atmane Jean-Yves Piquemal Lise-Marie Lacroix Guillaume Viau Universite de Toulouse LPCNO UMR 5215 INSA CNRS UPS 135 av de Rangueil 31077 Toulouse Cedex 4 France Laboratoire Leon Brillouin CEA/CNRS UMR 12 IRAMIS CEA-Saclay 91191 Gifsur Yvette France Department of Materials Science and Engineering University ofloannina Ioannina 45110 Greece Universite Paris Diderot Sorbonne Paris Cite ITODYS CNRS UMR 7086 15 rueJ.-A de Baif 75205 Paris Cedex 13 France

We present results of the synthesis of cobalt nanorods using the polyol process and the mechanism of magnetization reversal. We show that the nucleation step is significantly dependent on the nature of the ruthenium chloride used as the nucleating agent. This allows varying the diameter and aspect ratio of the cobalt nanorods independently. Co nanorods with aspect ratio, mean diameter, and mean length in the ranges ARm =3-16, Din= 7-25 nm, and Lm=30-300 nm, respectively, were produced using this method. X-ray diffraction and electron microscopy showed that a strong discrepancy between the structural coherence and morphological aspect ratio can exist because of stacking faults. The coercivity of assemblies of different nanorods was systematically measured, and the highest values were obtained for the smallest diameter and the largest structural coherence length. Micromagnetic simulations were performed to account for the dependence of the coercive field on the diameter. An important observation is that simple coherent magnetization rotation models do not apply to these magnetic nano-objects. Even for very small diameters （Dm = 5-10 nm） well below the theoretical coherent diameter Dcoh（CO）= 24 nm, we observed inhomogeneous reversal modes dominated by nucleation at the rod edges or at structural defects such as stacking faults. We conclude that, in order to produce high-coercivity materials based on nanowires, moderate aspect ratios of 5-10 are sufficient for providing a structural coherence similar to the morphological aspect ratio. Thus, the first priority should be to avoid the formation of stacking faults within the Co nanowires.

关键词： nanorod nanowire permanent magnets micromagnetic calculations shape anisoteopy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Catalytic Reduction of Benzaldehyde Under Hydrogen Flow over Nickel-Containing Mesoporous Silica Catalysts

引用

Open Journal of Physical Chemistry 2012年第1期2卷 73-80页

作者： Adel Saadi kahina Lanasri Khaldoun Bachari Djamila Halliche Chérifa Rabia Laboratoire de Chimie du Gaz Naturel Faculty of Chemistry University of Sciences and Technology Houari Boumediene Algiers Algeria

The hydrogenation of benzaldehyde over a series of nickel-containing mesoporous silicas with different nickel contents was studied at atmospheric pressure in the range temperature of 393 - 513 K under H2 ?ow. These materials (noted Nin-HMS with n = Si/Ni = 50, 25, 15) have been prepared at room temperature using a route based on hydrogen bonding and self-assembly between neutral primary amine micelles (S0) and neutral inorganic precursors (I0). They were characterized by their chemical analysis, BET surface area, XRD, FT-IR, and SEM microscopy. The obtained products were benzylalcohol, toluene, benzene with yields depending on the nickel content (Si/Ni ratio) and reaction temperature. The products of benzaldehyde hydrogenation (benzylalcohol, and toluene) and hydrogenolysis (benzene) were preferentially formed at low/middle and high reaction temperature respectively. The mesoporous Ni-containing materials were very active hydrogenation catalysts with almost 90% selectivity to benzylalcohol product and showed excellent stability. A mechanism in which the reaction could be initiated by a benzaldehyde reduction over Nin-HMS materials under hydrogen flow with formation of reaction products is proposed.

关键词： Nickel HMS Benzaldehyde Reduction Mesoporous Materials

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Visible Light Hydrogen Evolution over α-MoO3and α-MoO3/ZnO Hetero-Junction

引用

Open Journal of Physical Chemistry 2021年第3期11卷 144-156页

作者： kahina Bounache Amel Boudjemaa Souhila Boumaza Meriem Haddad Warda Tallas Akila Belhadi Mohamed Trari Laboratory of Chemistry of Natural gas Faculty of Chemistry University of Science and Technology Houari Boumediene Algiers Algeria Centre de Recherche Scientifique et Technique en Analyses Physico-Chimique Tipaza Algeria Laboratory of Storage and Valorization of Renewable Energies Faculty of Chemistry University of Science and Technology Houari Boumediene Algiers Algeria

MoO3 and 5% MoO3/ZnO were prepared by impregnation method using (NH4)6Mo7O24, 4H2O as precursor and ZnO as support. The prepared samples were characterized by X-ray diffraction (XRD), scanning electronic microscopy (SEM), infra red (FTIR) and UV-Vis diffuse reflectance (DRS) spectroscopies and photo-electrochemistry. The XRD pattern showed that the MoO3 powder treated at 700°C is a single-phase crystallizing in an orthorhombic structure with a direct optical transition (2.70 eV). The hetero-junction 5% MoO3/ZnO was photo-electrochemically characterized to assess its feasibility for H2 production under visible light. The capacitance potential (C-2 f(E)) characteristic of MoO3 plotted in Na2SO4, (0.1 M) electrolyte indicates n-type conduction with a flat band potential of -0.54 VSCE. The photocatalytic activity was performed for the photoreduction of water to hydrogen under visible light illumination. The best performance occurs at pH ~ 7 with an evolved volume of 5.9 mL.

关键词： MoO3/ZnO Hetero-Junction Photocatalysis Hydrogen Visible Light

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：