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Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression

Journal of Pharmaceutical Analysis 2018年第6期8卷 394-399页

作者： Ibrahim Turan Selim Demir kagan kilinc Serap Ozer Yaman Sema Misir Hanife Kara Berna Genc Ahmet Mentese Yuksel Aliyazicioglu Orhan Deger Department of Genetic and Bioengineering Faculty of Engineering and Natural Sciences Gumushane University Medicinal Plants Traditional Medicine Practice and Research Center Gumushane University Department of Nutrition and Dietetics Faculty of Health Sciences Karadeniz Technical University Department of Medical Biochemistry Faculty of Medicine Karadeniz Technical University Department of Biochemistry Faculty of Pharmacy Cumhuriyet University Program of Medical Laboratory Techniques Vocational School of Health Sciences Karadeniz Technical University

Rosa canina is a member of the genus Rosa that has long been used for medical objectives. Several studies have reported cytotoxic effects of different Rosa species, but there has been only limited investigation of the cytotoxic effect of R. canina. The purpose of the current study was to examine the potential effect of R.canina extract on cell viability, the cell cycle, apoptosis, and the expression of telomerase in human colon cancer(WiDr) cells. The cytotoxic effect of the extract was determined using MTT assay. The mechanism involved in the cytotoxic effect of the extract was then evaluated in terms of apoptosis and the cell cycle using flow cytometry. Mitochondrial membrane potential(MMP) was investigated using the fluorometric method, and expression levels of telomerase were studied using RT-PCR. R. canina extract exhibited a selective cytotoxic effect on WiDr cells compared with normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells. R. canina extract significantly repressed telomerase expressions at treatment times of 48 and 72 h in WiDr cells. Our results suggest that R. canina may have considerable potential for development as a novel natural product-based anticancer agent.

关键词： Apoptosis Colon cancer Cytotoxicity Rosa canina Telomerase

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