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Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on the Isolated Uterus of Mice

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Pharmacology & Pharmacy 2023年第8期14卷 252-270页

作者： Belem-kabré Wendkouni Leila Marie Esther Nitiéma Mathieu Odjo Blondine Saïdath Maman Noura Oumarou Yaro Boubacar Kaboré Boukaré Somda Gaëtan Donzéo Traoré Tata Kadiatou Koala Moumouni Ilboudo Sylvain Ouédraogo Moussa kabré elie Ouédraogo Noufou Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM) Institut de Recherche en Sciences de la Santé (IRSS/CNRST) Ouagadougou Burkina Faso Laboratoire du Développement du Médicament (LADME)/Centre de Formation de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Laboratoire de Chimie Organique et de Physique Appliquée (LCOPA) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Agence Nationale pour la Sécurité Sanitaire de l’Environnement de l’Alimentation du Travail et des Produits de Santé (ANSSEAT) Ouagadougou Burkina Faso

Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A2 was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation. No significant difference was noted in the phospholipase inhibition between the extracts and betamethasone. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC50 value of 55.82 ± 1.15 μg/mL. The lethal dose (LD50) of extracts was estimated to be superior to 2000 mg/kg body weight (bw). The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC50) was 5.33 × 10−8 ± 0.32 mg/mL for oxytocin, 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate childbirth.

关键词： Anastatica hierochuntica Phytoconstituents Antioxidant Anti-Inflammatory Uterine Contractility

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Ionic Disorders Observed in Infants with Severe Wasting at the Yalgado Ouedraogo Teaching Hospital and the Charles de Gaulle Pediatric Teaching Hospital in Ouagadougou, Burkina Faso

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Advances in Biological Chemistry 2023年第6期13卷 247-255页

作者： Arnaud Kouraogo Fabienne Soudre Caroline Yonaba Okengo Faouziatou Yagui Angele Kalmogho Ghislaine Yameogo Alice Kiba Raoul Karfo Ollo Da Emmanuel Zongo Cherileila Thiombiano Fatou Gueye Tall Abdoul Salam Ouédraogo Sanata Bamba Georges Anicet Ouédraogo elie kabré Fla Koueta Jean Sakande Biochemistry Laboratory of Souro Sanou Teaching Hospital Nazi Boni University Bobo-Dioulasso Burkina Faso Health Sciences Training and Research Unit Joseph Ki-Zerbo University Ouagadougou Burkina Faso Department of Pharmaceutical Biochemistry Faculty of Medicine Pharmacy and Dentistry Cheikh Anta Diop University Dakar Senegal

Introduction: A higher risk of death is associated with wasting in children if it is not treated properly. The objective of this study was to investigate the ionic disorders observed in infants suffering from severe wasting at Yalgado Ouedraogo Teaching Hospital (YO-TH) and at Charles de Gaulle Pediatric Teaching Hospital (CDG-PTH) in Ouagadougou (Burkina Faso). Methods: This was a retrospective study with a descriptive and analytical aim over the period from January 1, 2016 to December 31, 2020. Results: It concerned infants aged 6 to 24 months hospitalized at YO-TH and at CDG-PTH from Ouagadougou. We included 271 infants. The mean age of the infants was 14.48 ± 5.44 months with 42.07% which was in the age range of [12 - 18] months. On admission to the hospital, the children had an average weight, height and BMI of 6.22 ± 1.32 kg, 0.73 ± 0.07 m and 11.67 ± 1.53 kg/m2. In urban areas 56.46% of children and the main reasons for consultation were fever (88.19%), vomiting (52.80%) and diarrhea (50.20%). Electrolyte disturbances in emaciated infants affected all 8 parameters of the blood ionogram. However, the major disorders were 65.68% hyponatremia, 55.35% hypobicarbonatemia, 41.33% hypoprotidemia and 32.47% hypokalemia in infants aged 6 to 24 months. We found an association between diarrhea and residence with hypokalemia (p = 0.0000) and hypochloremia (p = 0.010), respectively. Conclusion: Severe acute wasting in infants 6 to 24 months of age remains a concern in the hospital setting. The frequency of biochemical disturbances is also high.

关键词： Severe Wasting Emaciation Infants over 6 - 24 Months Blood Ionogram Burkina Faso

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Frequency of Antibiotic Resistance of Escherichia coliand Klebsiella pneumoniaeby Production of TOHO-Type β-Lactamases at Saint Camille Hospital, Ouagadougou (Burkina Faso)

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Advances in Microbiology 2021年第12期11卷 713-722页

作者： Serge Sougué Amana Mètuor-Dabiré Djénéba Ouermi Yasmine Rahimatou Wend-Kouni Tiemtoré Bénao Stéphanie Lynseh Carine Sita Théodora Mahoukèdè Zohoncon Yasmine Aminata Bangré elie kabré Jacques Simporé Department of Biochemistry and Microbiology Faculty of Applied Science and Technology Biomolecular Research Centre (CERBA/LABIOGENE) UFR-SVT Joseph KI-ZERBO University Ouagadougou Burkina Faso National Public Health Laboratory of Ouagadougou Ouagadougou Burkina Faso University of Dedougou Dedougou Burkina Faso Saint Thomas Aquinas University (USTA) Saint Camille Hospital of Ouagadougou Ouagadougou Burkina Faso

Extended-spectrum β-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on the blaTEM, blaCTX-M and blaSHV genes in ESBL producing Enterobacteria, however, very few studies reported in the literature were related to blaCTX-M subgroup blaTOHO. TOHO enzymes were responsible for healthcare-associated infections in hospitals and in the community. In Burkina Faso, data related to these types of enzymes were scarce. The purpose of this study was to detect TOHO enzymes in Escherichia coli and Klebsiella pneumoniae in order to know the prevalence of infections related to bacterial resistance due to TOHO enzymes at Saint Camille Hospital of Ouagadougou (Burkina Faso). The study was conducted firstly by microbiological identification of ESBLs-producing by Escherichia coli and Klebsiella pneumoniae using API 20 E gallery;secondly the antibiogram was performed by the diffusion method and finally the molecular characterization was made by conventional PCR to search for the blaTOHO gene. The visualization of the specific bands was made using the ultraviolet lamp (Gene Flash) for the photography of the gels. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value Escherichia coli and 18 (46.2%) Klebsiella pneumoniae. Molecular characterization showed the presence of the blaTOHO gene in 25 bacterial strains (64.1%). It was therefore established in this study the existence of blaTOHO gene at Saint Camille Hospital in Ouagadougou in Burkina Faso. Our study made it possible to know the distribution of the blaTOHO gene in Escherichia coli and Klebsiella pneumoniae.

关键词： Antibiotic Resistance Bacteria ESBL Genes TOHO

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