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Aortic heterogeneity across segments and under high fat/salt/glucose conditions at the single-cell level

National Science Review 2020年第5期7卷 881-896页

作者： Dongxu He Aiqin Mao Chang-Bo Zheng Hao kan ka zhang Zhiming zhang Lei Feng Xin Ma Wuxi School of Medicine and School of Food Science and Technology Jiangnan University School of Biotechnology Jiangnan University School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University

The aorta, with ascending, arch, thoracic and abdominal segments, responds to the heartbeat, senses metabolites and distributes blood to all parts of the body. However, the heterogeneity across aortic segments and how metabolic pathologies change it are not known. Here, a total of 216 612 individual cells from the ascending aorta, aortic arch, and thoracic and abdominal segments of mouse aortas under normal conditions or with high blood glucose levels, high dietary salt, or high fat intake were profiled using single-cell RNA sequencing. We generated a compendium of 10 distinct cell types, mainly endothelial(EC), smooth muscle (SMC), stromal and immune cells. The distributions of the different cells and their intercommunication were influenced by the hemodynamic microenvironment across anatomical segments,and the spatial heterogeneity of ECs and SMCs may contribute to differential vascular dilation and constriction that were measured by wire myography. Importantly, the composition of aortic cells, their gene expression profiles and their regulatory intercellular networks broadly changed in response to high fat/salt/glucose conditions. Notably, the abdominal aorta showed the most dramatic changes in cellular composition, particularly involving ECs, fibroblasts and myeloid cells with cardiovascular risk factor-related regulons and gene expression networks. Our study elucidates the nature and range of aortic cell diversity,with implications for the treatment of metabolic pathologies.

关键词： aorta single-cell RNA sequencing cardiovascular disease

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Fine Structure of Scorpion Pectines for Odor Capture

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Journal of Bionic Engineering 2017年第4期14卷 589-599页

作者： Zhiwu Han Daobing Chen ka zhang Honglie Song Kejun Wang Shichao Niu Junqiu zhang Luquan Ren Key Laboratory for Bionic Engineering Ministry of Education Jilin University Changchun 130022 China

The paper revealed the fine structure of the scorpion （Mesobuthus martensii） pectines and showed how the fine structure of the pecten influences odor flow. The first step of our investigation was to prove that scorpion pectines work as olfactory and this was done via experiments utilizing paraffin coverage. Subsequently, the location, morphology, section structure, and arrange- ment of the pectines were studied via stereomicroscopy and Scanning Electron Microscopy （SEM）. The fine structure of pecten comprises a comb-like structure with 24-30 knife-like teeth and thousands of micron bowl-like pecten sensilla in staggered arrangement on the surface of the tooth. Computational Fluid Dynamics （CFD） was applied to predict odor flow around the pecten via the relevant Reynolds numbers. The comb-like structure amplified the odor flow velocity similar to an amplifier, transporting the odor flow of increased velocity to the micron pecten sensilla, improving transport efficiency of the odor flow. The staggered arrangement of the pecten sensilla generated a vortex, improving contact duration between pecten sensilla and odorant molecules. Thus, the pecten fine structure was likely acting as an effective comb with non-smooth teeth for the transport and capture of odorant molecules.

关键词： scorpion pectines fluid dynamics odor capture fine structure biomimetic

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Synthesis of zinc-based metal-organic framework as highly efficient photocatalyst for decomposition of organic dyes in aqueous solution

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Rare Metals 2023年第4期42卷 1205-1213页

作者： Geng Tan Yu-Qiong Guo Lu-Yang Zuo ka zhang Yi-Ming zhang Li-Lei zhang Jing-Jing Yu Xun Feng Bo Li Li-Ya Wang College of Chemistry and Pharmaceutical Engineering Nanyang Normal UniversityNanyang473061China College of Chemistry and Chemical Engineering Luoyang Normal UniversityLuoyang471934China

In the present work,a zinc-based metal-organic framework{[Zn(ddpd)_(0.5)(bipy)_(0.5)]·H_(2)O]}_(n)(1-Zn)(where ddpd=2,5-di(2',4'-dic arboxylphenyl)-1,4-difluorobenzene,bipy=4,4-bipyridine)was synthesized by a solvothermal *** 1-Zn features a threedimensional(2,4,6)-connected network with{4^2.6^2.8^2}_(2){4^4.6^6.8^5}{6}***,without the assistance of other reagents,complex 1-Zn not only exhibited an exceeded 95%photodegradation efficiency for rhodamine B(Rh B),methyl blue(MB)and methyl orange(MO)within 40 min,but also showed high stability and *** addition,free radical quenching experiments and electron spin resonance(ESR)spectroscopy verified that the main active species in the photodegradation process were·O_(2)^(-)and OH,and a more indepth degradation mechanism was further elucidated by density functional theory(DFT)calculations.

关键词： Zinc Metal-organic frameworks(MOFs) ·O_(2)^(-) ·OH Photodegradation

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Genome-wide identification and expression profiling of the YUCCA gene family in Brassica napus

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Oil Crop Science 2022年第3期7卷 103-111页

作者： ka zhang Jinfang zhang Cheng Cui Liang Chai Benchuan Zheng Jun Jiang Haojie Li Jinxing Tu Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu610066China National Key Laboratory of Crop Genetic Improvement National Sub-Center of Rapeseed Improvement in WuhanCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China

Auxin is essential for plant growth and development,also closely related to plant ***(YUC)proteins catalyse the rate-limiting step for endogenous auxin *** this study,we identified 57 Bn YUC genes in Brassica napus(rapeseed)genome,located on 17 *** them,A09 chromosome contained the most Bn YUCs as *** tree showed that all Bn YUC members were divided into 2 evolutionary clusters,including 7 *** the sub-groups,Clade I-C consisted 20 members,evolved independently from Arabidopsis thaliana,and motif 10 was *** collinearity analysis showed that YUC collinear gene pairs widely existed in Brassica species,indicating that this family has a high retention rate in the *** profile suggested that the Bn YUC family genes tended to express in specific growth/development stages as well as suffering stress/hormone *** suggested that dominant expression of specific members existed with each development stage or treatment,indicating that each Bn YUC might have a clear division of work,and play a given biological *** work was expected to build a basis for further study of the involvement of Bn YUC in growth,development and stress response.

关键词： Brassica napus YUCCA(YUC) Auxin biosynthesis Gene family Plant architecture

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Non-Metric CCD Camera Calibration Algorithm in a Digital Photogrammetry System

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Journal of China University of Mining and Technology 2006年第2期16卷 119-122页

作者： YANG Hua-chao DENG ka-zhong zhang Shu-bi GUO Guang-li ZHOU Ming School of Environment and Spatial lnformatics China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China

Camera calibration is a critical process in photogrammetry and a necessary step to acquire 3D information from a 2D image. In this paper, a flexible approach for CCD camera calibration using 2D direct linear transformation （DLT） and bundle adjustment is proposed. The proposed approach assumes that the camera interior orientation elements are known, and addresses a new closed form solution in planar object space based on homogenous coordinate representation and matrix factorization. Homogeneous coordinate representation offers a direct matrix correspondence between the parameters of the 2D DLT and the collinearity equation. The matrix factorization starts by recovering the elements of the rotation matrix and then solving for the camera position with the collinearity equation. Camera calibration with high precision is addressed by bundle adjustment using the initial values of the camera orientation elements. The results show that the calibration precision of principal point and focal length is about 0.2 and 0.3 pixels respectivelv, which can meet the requirements of close-range photogrammetry with high accuracy.

关键词： direct linear transformation collinearity equation bundle adjustment camera calibration Hough transformation

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Correlation of effective hepatic blood flow with liver pathology in patients with hepatitis B virus

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Liver Research 2021年第4期5卷 243-250页

作者： Xin Shu Haixia Sun Xiaoan Yang Yifan Jia Peiyan Xu Hong Cao ka zhang Department of Infectious Diseases The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina Department of Infectious Diseases Huadu District People's Hospital of GuangzhouGuangzhouChina

Background and aims:Effective hepatic blood flow(EHBF)decreases with liver disease progression,and identifying liver pathology is critical for patients with liver *** study was designed to elucidate the correlation between EHBF and liver pathology and explore the potential of EHBF for predicting the degree of liver ***:In this study,207 patients with hepatitis B virus(HBV)who underwent liver biopsy and indocyanine green(ICG)clearance tests were *** was measured using the ICG clearance test,and liver tissue was histologically analyzed to determine the pathological stage according to the Scheuer scoring *** data,biochemical indexes,and FibroScan data were collected for statistical ***:EHBF levels decreased as the liver histological stages of inflammation and fibrosis increased(Pkaline phosphatase,aspartate aminotransferase-to-platelet ratio index,fibrosis index based on the four factors,and liver stiffness measurement(P<0.05).The EHBF levels of patients without liver inflammation(G0)were significantly higher than those of patients with liver inflammation(G1e4)(P<0.001).The area under the receiver operating characteristic curve(AUROC)value for discriminating patients without liver inflammation was 0.827,and the optimal cutoff value was 0.936 L/*** EHBF levels of patients with severe liver inflammation(G4)were significantly lower than those of patients with G0e3 liver inflammation(P<0.001).The AUROC value for discriminating patients with severe liver inflammation was 0.792,and the optimal cutoff value was 0.552 L/*** EHBF levels of patients without liver fibrosis(S0)were significantly higher than those of patients with liver fibrosis(S1e4)(P<0.001).The AUROC value for discriminating patients without liver fibrosis was 0.633,and the optimal cutoff val

关键词： Hepatitis B virus(HBV) Liver inflammation Liver fibrosis Effective hepatic blood flow(EHBF) Liver disease

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TRPC5 is essential in endothelium-dependent contraction of aorta from diet-induced obese mice

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Fundamental Research 2022年第3期2卷 429-436页

作者： Yifei Zhu Sheng Wang Yuan Chu ka zhang Xin Wen Lei Feng Fan Yu Xin Ma Wuxi School of Medicine Jiangnan UniversityWuxi 214000China

The role of the Ca^(2+)-permeable ion channel TRPC5 in regulating vasocontraction in obesity is poorly ***,we investigated whether TRPC5 contributes to vascular dysfunction in obesity by promoting endothelium-dependent contraction via activation of cytosolic phospholipase A2(cPLA_(2))in the aortic endothelial cells of obese ***-induced endothelium-dependent relaxation and contraction in the aorta were measured us-ing wire ***_(2)activity was measured by the fluorogenic PLA_(2)substrate Bis-BODIPY^(TM)FL C_(11)-*** intracellular Ca^(2+)level in response to acetylcholine was measured by Fluo-4 ***-derived contracting factors were assessed by enzyme ***-induced obesity(DIO)attenuated endothelium-dependent vasodilation,enhanced endothelium-dependent contraction(EDC),and increased the expression of TRPC5 in the mouse *** of TRPC5 promoted EDC in the wild-type mouse aorta,whereas pharma-cological inhibition and genetic knockout of TRPC5 decreased EDC in the DIO mouse ***,cPLA_(2)phosphorylation and activity were higher in aortic endothelial cells from DIO mice,and this was attenuated by inhibition and knockout of *** 2(COX-2)expression was increased in DIO mouse endothe-lium and was decreased by a TRPC5 inhibitor and knockout of *** of prostaglandins F_(2α(PGF_(2α)and E 2(PGE 2)was involved in TRPC5-regulated EDC in DIO *** study demonstrated that TRPC5 contributes to endothelial and vascular dysfunction and is involved in EDC through activation of cPLA_(2)and enhanced COX-2-PGF_(2α)/PGE_(2)levels in DIO mice.

关键词： Transient receptor potential channel canonical family member 5(TRPC5) Endothelium-dependent contraction Vascular function Diet-induced obesity

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基于数字视差模型和改进SIFT特征的数字近景立体影像匹配

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测绘学报 2010年第6期39卷 624-630页

作者：张卡盛业华叶春南京师范大学虚拟地理环境教育部重点实验室江苏南京210046

针对数字近景立体摄影测量中立体影像的高效自动匹配的需要,提出一种基于数字视差模型(digital parallax model,DPM)和改进SIFT(scale invariable feature transformation)特征的立体影像匹配算法。该算法首先根据近景立体摄影系统中相... 详细信息

针对数字近景立体摄影测量中立体影像的高效自动匹配的需要,提出一种基于数字视差模型(digital parallax model,DPM)和改进SIFT(scale invariable feature transformation)特征的立体影像匹配算法。该算法首先根据近景立体摄影系统中相机基距和相互关系固定的特点,利用已知的标定数据,构建立体影像的数字视差模型,并计算立体影像中的同名核线参数,再在DPM和核线的约束下,确定右影像上同名像点的搜索范围,以提高匹配速度和准确性;其次,利用SIFT特征和灰度特征,设计结合局部结构信息和全局灰度信息的匹配相似性测度计算模型,以增强匹配的稳健性。利用该算法对实际立体影像进行试验,结果表明本文方法可大大消减错误匹配,提高匹配的可靠性和精度。

关键词：近景立体摄影测量影像匹配数字视差模型 SIFT特征核线

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基于复数矩的形状轮廓描述与匹配方法

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四川大学学报（工程科学版） 2011年第2期43卷 109-115页

作者：赵东保贺添张卡华北水利水电学院资源与环境学院河南郑州450011 郑州大学水利与环境学院河南郑州450001 南京师范大学虚拟地理环境教育部重点实验室江苏南京210046

建立合适的轮廓描述函数是各种形状轮廓匹配算法的前提所在。基于复数矩理论构造了轮廓顶点与形状旋转、缩放和平移无关的局部复数矩不变量,推导了局部复数矩与原始轮廓顶点坐标的正反算公式,将轮廓特征顶点处的局部复数矩和曲率合在一... 详细信息

建立合适的轮廓描述函数是各种形状轮廓匹配算法的前提所在。基于复数矩理论构造了轮廓顶点与形状旋转、缩放和平移无关的局部复数矩不变量,推导了局部复数矩与原始轮廓顶点坐标的正反算公式,将轮廓特征顶点处的局部复数矩和曲率合在一起,从而建立了一种新的轮廓描述函数。该轮廓描述函数较为充分地考虑到了轮廓的全局与局部特征,除了具有不变性和唯一性等性质外,也具有较高的计算效率,仅需花费线性时间即可完成轮廓描述函数的构造,由于仅涉及到低阶几何矩,其局部复数矩也具有较强的抗噪能力。基于该轮廓描述函数,利用串匹配算法实现了形状的轮廓匹配,与现有基于各种轮廓描述函数的匹配方法的实验对比表明,本文轮廓描述函数及其匹配方法具有更为优秀的匹配性能。

关键词：形状匹配轮廓描述函数复数矩

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HBV蛋白诱导促炎症细胞因子白细胞介素32表达的研究

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中华肝脏病杂志 2013年第6期21卷 442-445页

作者：曹红中山大学附属第三医院感染性疾病科广州510630

目的研究HBV是否诱导白细胞介素32（IL-32）表达及其机制。方法用脂质体将HBV表达载体pIRES2-HBV-EGFP转染HepG2细胞，48h后实时定量PCR、酶联免疫吸附法、Westemblot分别在基因及蛋白质水平检测IL-32表达；核因子K0亚单位pS0、p65蛋白... 详细信息

目的研究HBV是否诱导白细胞介素32（IL-32）表达及其机制。方法用脂质体将HBV表达载体pIRES2-HBV-EGFP转染HepG2细胞，48h后实时定量PCR、酶联免疫吸附法、Westemblot分别在基因及蛋白质水平检测IL-32表达；核因子K0亚单位pS0、p65蛋白分别与HepG2细胞共培养，48h后在基因及蛋白质水平检测IL-32表达。HBV表达载体plRES2-HBV-EGNP转染HepG2细胞，然后加入核因子1cB抑制剂SN50，48h后Westemblot检测IL-32表达水平。统计学分析采用SPSSl6．0软件，用f检验比较组间差异。结果转染HBV表达载体的细胞IL-32表达水平高于转染空载体的细胞，实时定量PCR检测示转染HBV表达载体组IL-32表达水平为空载体组的2．8倍，ELISA检测示转染HBV表达载体组IL-32表达水平为空载体组的4．2倍，两组之间的差异有统计学意义垆=0．002）；核因子Kp亚单位p50、p65蛋白可诱导HepG2细胞表达IL-32（P=0．001）；核因子K0抑制剂SN50抑制HBV表达载体诱导IL-32表达。结论HBV诱导肝细胞表达IL-32，IL-32可能参与HBV感染引起的病毒性肝炎的发病机制。

关键词：肝炎病毒乙型白细胞介素32 HBV感染 HepG2细胞

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