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The effects of detergents DDM and β-OG on the singlet excited state lifetime of the chlorophyll a in cytochrome b_6f complex from spinach chloroplasts

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Science China(Life Sciences) 2007年第4期50卷 505-510页

作者： CHEN XiaoBo1,3, ZHAO XiaoHui2, ZHANG JianPing2, LI LiangBi1& kuang tingyun1 1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2 State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sci- ences, Beijing 100080, China 3 Graduate University of Chinese Academy of Sciences, Beijing 100039, China Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of SciencesBeijing 100093 China Graduate University of Chinese Academy of Sciences Beijing 100039 China State Key Laboratory for Structural Chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of SciencesBeijing 100093 China

The singlet excited state lifetime of the chlorophyll a (Chl a) in cytochrome b6f (Cyt b6f) complex was reported to be shorter than that of free Chl a in methanol, but the value was different for Cyt b6f com-plexes from different sources (~200 and ~600 ps are the two measured results). The present study demonstrated that the singlet excited state lifetime is associated with the detergents n-dodecyl-β-D- maltoside (DDM) and n-octyl-β-D-glucopyranoside (β-OG), but has nothing to do with the different sources of Cyt b6f complexes. Compared with the Cyt b6f dissolved in β-OG, the Cyt b6f in DDM had a lower fluorescence yield, a lower photodegradation rate of Chl a, and a shorter lifetime of Chl a excited state. In short, the singlet excited state lifetime, ~200 ps, of the Chl a in Cyt b6f complex in DDM is closer to the true in vivo.

关键词： cytochrome b6f detergent chlorophyll a singlet excited state lifetime singlet oxygen

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The guanidine hydrochloride-induced denaturation of CP43 and CP47 studied by terahertz time-domain spectroscopy

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Science China(Life Sciences) 2007年第3期50卷 350-355页

作者： QU YuanGang1,3, CHEN Hua2, QIN XiaoChun1, WANG Li2, LI LiangBi1 & kuang tingyun1 1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2 Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China 3 Graduate University of Chinese Academy of Sciences, Beijing 100049, China Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of SciencesBeijing 100093 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Laboratory of Optical Physics Institute of Physics and Center for Condensed Matter Physics Chinese Academy of SciencesBeijing 100080 China Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of SciencesBeijing 100093 China

Terahertz time-domain spectroscopy (THz-TDS) is a new technique in studying the conformational state of a molecule in recent years. In this work, we reported the first use of THz-TDS to examine the dena- turation of two photosynthesis membrane proteins: CP43 and CP47. THz-TDS was proven to be useful in discriminating the different conformational states of given proteins with similar structure and in monitoring the denaturation process of proteins. Upon treatment with guanidine hydrochloride (GuHCl), a 1.8 THz peak appeared for CP47 and free chlorophyll a (Chl a). This peak was deemed to originate from the interaction between Chl a and GuHCl molecules. The Chl a molecules in CP47 interacted with GuHCl more easily than those in CP43.

关键词： CP The guanidine hydrochloride-induced denaturation of CP43 and CP47 studied by terahertz time-domain spectroscopy THz time

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