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Cryo-electron microscopy structure of the intact photosynthetic light-harvesting antenna-reaction center complex from a green sulfur bacterium

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Journal of Integrative Plant Biology 2023年第1期65卷 223-234页

作者： Jing-Hua Chen Weiwei Wang Chen Wang tingyun kuang Jian-Ren Shen Xing Zhang College of Life Science Zhejiang University Hangzhou 310058 China Department of Pathology of Sir Run Run Shaw Hospital and Department of Biophysics Zhejiang University School of MedicineHangzhou 310058 China Key Laboratory of Photobiology Institute of BotanyPhotosynthesis Research Center the Chinese Academy of SciencesBeijing 100093 China Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology Okayama UniversityOkayama 700-8530 Japan Center of Cryo Electron Microscopy Zhejiang University School of Medicine Hangzhou 310058 China

The photosynthetic reaction center complex(RCC)of green sulfur bacteria(GSB)consists of the membrane-imbedded RC core and the peripheric energy transmitting proteins called Fenna–Matthews–Olson(FMO).Functionally,FMO transfers the absorbed energy from a huge peripheral light-harvesting antenna named chlorosome to the RC core where charge separation *** vivo,one RC was found to bind two FMOs,however,the intact structure of RCC as well as the energy transfer mechanism within RCC remain to be *** we report a structure of intact RCC which contains a RC core and two FMO trimers from a thermophilic green sulfur bacterium Chlorobaculum tepidum at 2.9A resolution by cryo-electron *** second FMO trimer is attached at the cytoplasmic side asymmetrically relative to the first FMO trimer reported *** also observed two new subunits(PscE and PscF)and the N-terminal transmembrane domain of a cytochrome-containing subunit(PscC)in the *** two novel subunits possibly function to facilitate the binding of FMOs to RC core and to stabilize the whole complex.A new bacteriochlorophyll(numbered as 816)was identified at the interspace between PscF and PscA-1,causing an asymmetrical energy transfer from the two FMO trimers to RC *** on the structure,we propose an energy transfer network within this photosynthetic apparatus.

关键词： cryo-electron microscopy energy transfer FMO protein green sulfur bacterium photosynthesis reaction center

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A breakthrough of artificial photosynthesis

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National Science Review 2016年第1期3卷 2-3页

作者： tingyun kuang Photosynthesis Research Center Key Laboratory of PhotobiologyInstitute of BotanyChinese Academy of Sciences

Photosynthesis in plants,algae and cyanobacteria uses light energy from the Sun to convert CO2 and water into carbohydrates and oxygen,thus sustaining all aerobic life forms on *** water splitting into oxygen,protons ... 详细信息

关键词： OEC PSII

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A new milestone for photosynthesis

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National Science Review 2018年第4期5卷 444-445页

作者： Chunxi Zhang tingyun kuang Laboratory of Photochemistry Institute of Chemistry Chinese Academy of Sciences Laboratory of Photobiology Institute of BotanyChinese Academy of Sciences

The photosynthetic water-splitting reaction catalysed by the oxygen-evolving center(OEC)in the photosystem II of plants releases protons,electrons and dioxygen,which is one of the most important processes for energy and material conversions on *** is a Mn4Cacluster and cycles through five different states(Sn,n=0–4),wherein S0is the most reduced state,S1is the dark stable state and S2,S3and S4are intermediate

关键词： OEC A new milestone for photosynthesis

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Comparative Analysis of the Endosperm Proteins Separated by 2-D Electrophoresis for Two Cultivars of Hybrid Rice （Oryza sativa L,）

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Journal of Integrative Plant Biology 2006年第9期48卷 1028-1033页

作者： Pingfang Yang Shihua Shen tingyun kuang Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Liangyoupeijiu is a two-parental-line, and Shanyou63 is a three-parental-line hybrid rice （Oryza sativa L.）. Although both belong to the indica subspecies, they have obvious differences with respect to morphology, physiology and grain quality. Variations in endosperm protein compositions were studied by comparing the 2-D electrophoresis （2-DE） maps for these two cultivars of hybrid rice. After matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF/MS） analysis, a 21-kDa precursor of 19- kDa globulin was identified as the major storage protein for both cultivars. Some isoforms of peroxiredoxin and seed maturation protein were found to only exist in Shanyou63, whereas aldose reductase and starch granule-bound starch synthase were only detected in Liangyoupeijiu. These data might provide a foundation for further comparative studies of these two cultivars of hybrid rice.

关键词： endosperm hybrid rice mass spectrometry matrix assisted laser desorption/ionization time of flight 2-D electrophoresis

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Commitment and dedication of a Chinese plant physiologist

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Protein & Cell 2010年第10期1卷 886-887页

作者： tingyun kuang Ming Li Le Kang Institute of Botany Chinese Academy of SciencesBeijing 100093China Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China

Professor Peisong Tang(Pei-sung Tang),born in 1903,is one of the co-founders of the modern Chinese plant *** might be familiar with some of his academic achievements:he is the first one to discover respiratory enzymes(cytochorome oxidase)in plants;he proved that multiple respiratory metabolic pathways and electron transfers exist in the rice;he is the first one to experimentally prove the existence of carbonic anhydrase in plants;together with *** Wang,he used thermodynamical method to illustrate the mechanism of intracellular water movement,which later has been referred to as the“Tang-Wang theory of cellular water potential”.You probably also know that he is the first Chinese biologist who published in Nature and Science in 1940s and *** there is something about him that you will not learn from his over 200 publications:his commitment,as a Chinese,to his country and his people,and his dedication,as a scientist,in pursuing science and truths.

关键词： illustrate something probably

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Function of terahertz spectra in monitoring the decomposing process of biological macromolecules and in investigating the causes of photoinhibition

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Science China(Life Sciences) 2017年第3期60卷 307-312页

作者： Yuangang Qu Shuai Zhang Yuji Lian tingyun kuang College of Life Sciences Linyi University Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences

Chlorophyll α and β-carotene play an important role in harvesting light energy, which is used to drive photosynthesis in plants. In this study, terahertz(THz) and visible range spectra of chlorophyll α and β-carotene and their changes under light treatment were investigated. The results show that the all THz transmission and absorption spectra of chlorophyll α and β-carotene changed upon light treatment, with the maximum changes at 15 min of illumination indicating the greatest changes of the collective vibrational mode of chlorophyll α and β-carotene. The absorption spectra of chlorophyll α in the visible light region decreased upon light treatment, signifying the degradation of chlorophyll a molecules. It can be inferred from these results that the THz spectra are very sensitive in monitoring the changes of the collective vibrational mode, despite the absence of changes in molecular configuration. The THz spectra can therefore be used to monitor the decomposing process of biological macromolecules; however, visible absorption spectra can only be used to monitor the breakdown extent of biological macromolecules.

关键词：生物大分子光谱监测分解过程太赫兹可见光吸收光谱 β-胡萝卜素光抑制叶绿素a

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Changes in Thermostability of Photosystem Ⅱ and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes

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Journal of Integrative Plant Biology 2007年第4期49卷 515-522页

作者： Yunlai Tang Mei Chen Yinong Xu tingyun kuang College of Life Science and Engineering Southwest University of Science and Technology Mianyang 621000 China Photosynthesis Research Center Institute of Botany the Chinese Academy of Sciences Beijing 100093 China

We studied the difference in thermostability of photosystem Ⅱ （PSII） and leaf lipid composition between a T-DNA insertion mutant rice （Oryza sativa L.） VG28 and its wild type Zhonghuau. Native green gel and SDS-PAGE electrophoreses revealed that the mutant VG28 lacked all light-harvesting chlorophyll a/b protein complexes. Both the mutant and wild type were sensitive to high temperatures, and the maximal efficiency of PSII photochemistry （FJ Fm） and oxygen-evolving activity of PSII in leaves significantly decreased with increasing temperature. However, the PSII activity of the mutant was markedly more sensitive to high temperatures than that of the wild type. Lipid composition analysis showed that the mutant had less phosphatidylglycerol and sulfoquinovosyl diacylglycerol compared with the wild type. Fatty acid analysis revealed that the mutant had an obvious decrease in the content of 16：1t and a marked increase in the content of 18：3 compared with the wild type. The effects of lipid composition and unsaturation of membrane lipids on the thermostability of PSII are discussed.

关键词： high temperature lipid composition light-harvesting a/b protein complexes photosystem II rice.

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Phytochrome controlled, long-day photoperiod-inducible protein in rice leaves

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Chinese Science Bulletin 2000年第20期45卷 1874-1877页

作者： Bin Kang Zhe Tong tingyun kuang Chinese Acad Sci Inst Bot Beijing 100093 Peoples R China

A new protein in the leaves of NK58S and NK58 (Oryza sativa L. subsp. japonica), which can be induced by 10 d-long-day photoperiod (14 h light/d) and cannot be induced by 10 d-short-day photoperiod (10 h light/d), has been found by two-dimensional gel electrophoresis. The *** molecular weight and isoelectric point are 36 ku and pH 5.2 respectively, is found to be controlled by phytochrome as shown by the experiment of red light induction-far red light *** existence of this protein in both NK58S and NK58 reflects that some of the responses of NK58S and NK58 might be similar in response to long-day photoperiod, a mild stress.

关键词： rice protein photoperiod phytochrome.

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New structure model of oxygen-evolving center and mechanism for oxygen evolution in photosynthesis

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Chinese Science Bulletin 1999年第24期44卷 2209-2215页

作者： Chunxi Zhang Jie Pan Liangbi Li tingyun kuang Chinese Acad Sci Inst Bot Photosynth Res Ctr Beijing 100093 Peoples R China

So far, many important questions and problems concerning the structure and mechanism of photosynthetic oxygen evolution are still unsolved. On the basis of recent achievements in this field, a new structure model is proposed whereby two H2O molecules bind asymmetrically to two manganese ions (Mn1Ⅱ and Mn4Ⅲ) at the open end of 'C' shaped cluster and keep rather large distance. Two histidine residues coordinate to the other two manganese ions in higher oxi-

关键词： photosynthesis photosystem Ⅱ . oxygen-evolving center mechanism of oxygen evolution.

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Mutagenesis of Ser^(24) of cytochrome b559 α subunit affects PSII activities in Chlamydomonas reinhardtii

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Chinese Science Bulletin 2007年第7期52卷 896-902页

作者： MA JingJing LI LiangBi JING YuXiang kuang tingyun Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

In order to study the functions of cytochrome b559 (Cyt b559) in photosystem two (PSII) activity, mutant S24F of Chlamydomonas reinhardtii was constructed using site directed mutagenesis, in which Serine24 (Ser24) locating downstream of Histidine23 (His23) in α subunit of Cyt b559 was replaced by Phenylalanine (Phe). Physiological and biochemical analysis showed that mutant S24F could be grown photoautotrophically or photoheterotrophically. However, their growth rate was slower either on HSM or TAP medium than that of the control; Analysis of PSII activity revealed that its oxygen evolution was about 71% of wild type (WT); The Photochemical efficiency of PSII (Fv/Fm) of S24F was reduced 0.23 compared with WT; S24F was more sensitive to strong light irradiance than the wild type; Furthermore, SDS-PAGE and Western-blotting analysis indicated that the expression levels of α subunit of Cyt b559, LHCII and PsbO of S24F were a little less than those of the wild type. Overall, these data suggests that Ser24 plays a significant role in making Cyt b559 structure maintain PSII complex activity of oxygen evolution although it is not directly bound to heme group.

关键词：莱茵衣藻细胞色素b559α亚基丝氨酸残基 Ser24 突变光系统II 光合活性

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