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Hepatitis B virus subgenotype A1 predominates in liver disease patients from Kerala,India

World Journal of Gastroenterology 2013年第48期19卷 9294-9306页

作者： Deepak Gopalakrishnan Mark Keyter Kotacherry Trivikrama Shenoy Kondarappassery Balakumaran Leena Lakshmikanthan Thayumanavan Varghese Thomas kr vinayakumar Charles Panackel Arun T Korah Ramesh Nair Anna kramvis Hepatitis Virus Diversity Research Programme Department of Internal Medicine School of Clinical Medicine Faculty of Health Sciences University of the Witwatersrand Parktown Johannesburg 2193 South Africa Department of Gastroenterology Medical College Trivandrum 695011 India Population Health and Research Institute Trivandrum 695011 India Government Rajaji Hospital Madurai Medical College Madurai 625002 India Department of Gastroenterology Calicut Medical College PO Calicut 673008 India

AIM:To molecularly characterize hepatitis B virus(HBV)isolates from Kerala and to relate them to the clinical manifestation of ***:Sera and clinical data were collected from91 patients diagnosed with chronic HBV infection and HBV-related hepatocellular carcinoma(HCC).HBV from44 HCC,22 cirrhotic and 25 chronic hepatitis patients were genotyped by sequencing of the complete S region or by restriction fragment length polymorphism *** basic core promoter/precore region was *** complete surface DNA sequences were assembled and aligned manually,and then compared with the sequences of HBV of genotypes(A-J)from *** evolutionary history was inferred using the Neighbor-Joining method and the evolutionary distances computed using the Kimura 2-parameter *** was performed using 1000 *** TaqMan BS-1 probe was used to quantify HBV DNA at a lower detection limit of approximately20 IU/*** variables were compared using an independent Student’s t ***χ2test or Fisher’s exact test was used to compare categorical *** differences were considered statistically significant at P<***:Irrespective of disease status,the predominant genotype was A(72%);95%belonging to subgenotype A1,followed by genotypes D(27%)and C(1%).HCC patients infected with subgenotype A1were significantly younger than those infected with *** A1762T/G1764A was significantly associated with HCC in both genotypes A and *** G1862T was more frequent in subgenotype A1(P<0.0001),and in combination with A1762T/G1764A,it was significantly associated with HBV from HCC *** C1766T/T1768A was significantly associated with genotype A(P=0.05)and HCC(P=0.03).The preS2start codon M1T/I mutation was unique to genotype A strains(15.6%)from all disease groups and occurred at a higher frequency in isolates from HCC patients(P=0.076).A higher frequency of preS deletion mutants(33.3%)was observed in genotype A from HC

关键词： Hepatocellular carcinoma Cirrhosis Chronic hepatitis Phylogenetic analysis Genotype India

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