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猴痘病毒B20R蛋白原核表达及其多克隆抗体的制备

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南方农业学报 2024年第1期55卷 217-225页

作者：杨领弟李小梅芦晓鸥彭棋孔令保王宇江西农业大学生物科学与工程学院江西南昌330045 南昌市动物病毒与基因工程重点实验室江西南昌330045

【目的】构建猴痘病毒B20R蛋白原核表达系统,并评价原核表达获得的融合蛋白免疫原性,为后续的猴痘病毒检测及新型治疗方法开发提供技术支撑。【方法】参照GenBank已公布的猴痘病毒基因组序列,按照大肠杆菌密码子偏好性对B20R基因DNA序... 详细信息

【目的】构建猴痘病毒B20R蛋白原核表达系统,并评价原核表达获得的融合蛋白免疫原性,为后续的猴痘病毒检测及新型治疗方法开发提供技术支撑。【方法】参照GenBank已公布的猴痘病毒基因组序列,按照大肠杆菌密码子偏好性对B20R基因DNA序列进行优化,人工合成B20R基因并将其分别克隆至表达载体pET-28a和pET-32a以构建原核表达载体;以构建的2种原核表达载体分别转化大肠杆菌BL21感受态细胞,经IPTG诱导表达后使用SDSPAGE和Western blotting检测融合蛋白B20R的表达情况。通过控制变量法优化融合蛋白B20R的诱导表达条件,使用Ni柱亲和层析进行纯化;以纯化的融合蛋白B20R经腹腔注射免疫昆明小鼠,定期采集昆明小鼠血样,采用ELISA检测血清抗体效价。【结果】将B20R基因克隆至表达载体pET-32a能成功构建原核表达载体pET-32a-B20R,经IPTG诱导后,可在大肠杆菌BL21感受态细胞中表达出融合蛋白B20R,且主要以不可溶的包涵体形式进行表达。融合蛋白B20R最佳诱导表达条件为37℃下以0.6 mmol/L IPTG诱导12 h,Ni柱亲和层析纯化的最佳咪唑洗脱浓度为40 mmol/L。以纯化的融合蛋白B20R接种免疫昆明小鼠,可在短时间内引起昆明小鼠产生强烈的体液免疫,且至免疫第42 d仍然维持较高的抗体水平,抗体效价高达1∶409600。【结论】构建的猴痘病毒B20R蛋白原核表达载体可在大肠杆菌BL21细胞中以包涵体形式成功表达出融合蛋白B20R,且融合蛋白B20R具有良好的免疫原性,可在短时间内引起昆明小鼠产生强烈的体液免疫。

关键词：猴痘病毒 B20R蛋白原核表达免疫原性抗体效价

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基于空间分辨法分析时间低相干光自聚焦效应

物理学报 2024年第9期73卷 36-45页

作者：单翀孔令豹崔勇季来林赵晓晖李福建饶大幸赵元安隋展邵建达复旦大学信息科学与工程学院超精密光学制造工程技术研究中心上海201203 中国工程物理研究院上海激光等离子体研究所上海201800 中国科学院上海光学精密机械研究所强激光材料重点实验室上海201800

时间低相干光由于其瞬时宽带的物理特性,在激光惯性约束聚变中得到了广泛的关注.然而其复杂的时间尖峰结构或将诱导非线性自聚焦效应的放大.同时,传统的非线性自聚焦特征数值的测试方法中,多数材料的表面损伤先于体内自聚焦成丝损伤发生... 详细信息

时间低相干光由于其瞬时宽带的物理特性,在激光惯性约束聚变中得到了广泛的关注.然而其复杂的时间尖峰结构或将诱导非线性自聚焦效应的放大.同时,传统的非线性自聚焦特征数值的测试方法中,多数材料的表面损伤先于体内自聚焦成丝损伤发生,这为对比不同激光的非线性效应带来巨大影响.本文利用短焦距透镜对熔石英进行紧聚焦,通过调节入射激光能量,在避免前表面损伤的前提下,诱导熔石英产生自聚焦成丝损伤.随后通过理论计算对光束在样品体内传输过程的光斑变化进行空间分辨处理,并得到对应细分位置的非线性数值.最终将各个位置对应的非线性相位变化值进行积分,得到前表面无损条件下材料的非线性自聚焦特征数值.测试结果表明时间低相干光的非线性自聚焦效应比传统单模脉冲激光更强.本文不仅设计了一套更加精确的对比不同激光非线性效应的测试方法,同时也探明了时间相干性对于非线性自聚焦效应的影响机制,为高功率时间低相干激光器的设计提供理论依据和参考.

关键词：惯性约束核聚变时间低相干光非线性自聚焦特征数值空间分辨法

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The Comprehensive Retrieval Method of Electromagnetic Parameters Using the Scattering Parameters of Metamaterials for Two Choices of Time-Dependent Factors

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Chinese Physics Letters 2012年第1期29卷 242-245页

作者： HOU Zhi-ling kong ling-bao JIN Hai-Bo CAO Mao-Sheng LI Xiao QI Xin School of Science Beijing University of Chemical TechnologyBeijing 100029 School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081

The electromagnetic parameters (permittivity and permeability) method,retrieved from the reflection and transmission coefficients of a slab,is *** over existing methods,including the determination of the permittivity,permeability and impedance of the slab,are expressed as explicit functions of the S parameters for both the time-dependent factors,e^(iwt) and e^(-iwt) (ω is the angular frequency of the incident electromagnetic wave),and the proper selection of the sign of impedance and the real part of the refractive ***,based on the retrieving method,the calculations of the electromagnetic parameters of the conventional-material teflon slab standard sample through the experimental data of the S parameters are performed,which confirm the validity of the technique for the retrieval of electromagnetic parameters.

关键词： impedance permittivity validity

基于室内可见光通信技术的新型两级光学接收天线设计与分析

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物理学报 2018年第9期67卷 96-106页

作者：彭星孔令豹复旦大学信息科学与工程学院上海超精密光学制造工程技术研究中心上海200433

针对室内可见光通信系统的应用需求,设计了一种新型两级光学接收天线.根据复合抛物面聚光器光学增益随视场增大而减小的聚光特性,将复合抛物面聚光器截面基准曲线旋转设计得到具有一定旋转角与厚度的透镜壁复合抛物面聚光器.进一步结合... 详细信息

针对室内可见光通信系统的应用需求,设计了一种新型两级光学接收天线.根据复合抛物面聚光器光学增益随视场增大而减小的聚光特性,将复合抛物面聚光器截面基准曲线旋转设计得到具有一定旋转角与厚度的透镜壁复合抛物面聚光器.进一步结合透镜壁复合抛物面聚光器和半球透镜的优势设计了一种新型两级光学接收天线,在增益保持的情况下视场角增大了近20~?.在一个5 m×5 m×3 m的空旷房间,通过Trace Pro建立光学天线的分析模型,Matlab软件对室内可见光通信系统进行信道建模.计算结果表明,采用这种新型两级光学接收天线,与直接接收的情况相比,平均接收功率增幅为757.2%,是复合抛物面聚光器的5.62倍;信噪比平均值增幅为28.07%,是复合抛物面聚光器的1.67倍;光学增益为11.49,是复合抛物面聚光器的2.81倍.且光斑半径仅为2.5 mm,较复合抛物面聚光器减小了近37.5%,使得能量集中均匀分布,进一步证实该新型两级光学接收天线适用于室内可见光通信系统.

关键词：可见光通信新型两级光学接收天线增益视场

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Light(Science & Applications) 2024年第2期13卷 333-346页

作者： Yifan Sun Qian Li ling-Jun kong Xiangdong Zhang Key Laboratory of advanced optoelectronic quantum architecture and measurements of Ministry of Education Beijing Key Laboratory of Nanophotonics&Ultrafine Optoelectronic SystemsSchool of PhysicsBeijing Institute of Technology100081BeijingChina

Compared with electrical neural networks,optical neural networks(ONNs)have the potentials to break the limit of the bandwidth and reduce the consumption of energy,and therefore draw much attention in recent *** far,several types of ONNs have been ***,the current ONNs cannot realize the acceleration as powerful as that indicated by the models like quantum neural *** to construct and realize an ONN with the quantum speedup is a huge ***,we propose theoretically and demonstrate experimentally a new type of optical convolutional neural network by introducing the optical *** is called the correlated optical convolutional neural network(COCNN).We show that the COCNN can exhibit“quantum speedup”in the training *** character is verified from the two *** is the direct illustration of the faster convergence by comparing the loss function curves of the COCNN with that of the traditional convolutional neural network(CNN).Such a result is compatible with the training performance of the recently proposed quantum convolutional neural network(QCNN).The other is the demonstration of the COCNN’s capability to perform the QCNN phase recognition circuit,validating the connection between the COCNN and the ***,we take the COCNN analog to the 3-qubit QCNN phase recognition circuit as an example and perform an experiment to show the soundness and the feasibility of *** results perfectly match the theoretical *** proposal opens up a new avenue for realizing the ONNs with the quantum speedup,which will benefit the information processing in the era of big data.

关键词： process quantum convolution

Electrolyte effect on electrochemical behaviors of manganese fluoride material for aqueous asymmetric and symmetric supercapacitors

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Rare Metals 2024年第3期43卷 1048-1061页

作者： Zheng-Hua He Jian-Fei Gao ling-Bin kong State Key Laboratory of Advanced Processing and Recycling of Non-Ferrous Metals Lanzhou University of TechnologyLanzhou730050China School of Materials Science and Engineering Lanzhou University of TechnologyLanzhou730050China

Exploring wide voltage window materials is not only an available measure to enhance the energy density of hybrid supercapacitor(HSCs),but also avoids the dynamic mismatch caused by different energy storage mechanisms of two electrodes in assembled symmetrical ***,there are few reports about the wide potential window materials except Bi_(2)O_(3)and VO_(2).Therefore,the MnF_(2)synthesized by solvothermal reaction was served as the electrode for *** MnF_(2)exhibited electrochemical activity in alkaline solution in three-electrode system,especially with a wide potential window from-0.8 to+0.5 V in 2 mol·L^(-1)***,the assembled MnF_(2)//MnF_(2)symmetrical HSC had a potential window of 1.5 V,and it exhibited outstanding long-cycle ***,when MnF_(2)was taken as the negative and positive respectively,the potential windows of asymmetric devices CoMoO_(4)//MnF_(2)and MnF_(2)//Activated Carbon(AC)could reach 1.3 and 1.45 V,respectively,showing excellent cycle *** work shows that MnF_(2)material has great research value in HSC,and provides a new research direction for developing high-performance devices.

关键词： Manganese fluoride Potential window Hybrid supercapacitors Electrochemical performance

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An isolated symmetrical 2T2R cell enabling high precision and high density for RRAM-based in-memory computing

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Science China(Information Sciences) 2024年第5期67卷 287-294页

作者： Yaotian ling Zongwei WANG Yuhang YANG Lin bao Shengyu bao Qishen WANG Yimao CAI Ru HUANG School of Integrated Circuits Beijing Advanced Innovation Center for Integrated Circuits Peking University State Key Laboratory of Information Photonics and Optical Communications Beijing University of Posts and Telecommunications

In-memory computing(IMC), leveraging emerging memories, holds significant promise in overcoming memory limitations and improving energy efficiency. However, the prevailing IMC array structure based on serially connected transistors and memory cells(1T1R/2T2R), along with the signed weight mapping scheme, can lead to asymmetrical weight sensing issues(AWS) due to electrical asymmetry within the1T1R/2T2R structure, particularly in highly scaled cells where the transistor's resistance becomes significant. In this paper, we propose and fabricate an electrically symmetric memory cell based on a physically isolated 2T2R structure for IMC. This design aims to enhance the precision and density of RRAM-based IMC arrays. The 2T2R cells are manufactured using the back-end-of-line(BEOL) process of a commercial40 nm technology platform. The feasibility of this design is verified through measured and simulated results,showcasing its capability to address the issue of AWS. Compared to conventional 2T2R cells, this design achieves a considerably smaller transistor footprint without compromising accuracy, while also improving integration density by 42.2%. These innovative memory cell advancements have the potential to further advance high-energy-efficient IMC technology.

关键词： RRAM 2T2R multi-level storage weight asymmetry in-memory computing

Vitamin D binding protein in psychiatric and neurological disorders:Implications for diagnosis and treatment

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Genes & Diseases 2024年第5期11卷 324-334页

作者： ling Li Bing Han Yan kong Gaojia Zhang Zhijun Zhang Research Institution of Neuropsychiatry School of MedicineSoutheast UniversityNanjingJiangsu 210009China Department of Neurology Affiliated Zhongda HospitalNanjingJiangsu 210009China Department of Pharmacology Jiangsu Provincial Key Laboratory of Critical Care MedicineSchool of MedicineSoutheast UniversityNanjingJiangsu 210009China Department of Biochemistry and Molecular Biology School of MedicineSoutheast UniversityNanjingJiangsu 210009China Brain Cognition and Brain Disease Institute Department of Mental Health and Public HealthFaculty of Life and Health SciencesShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong 518055China

Vitamin D binding protein(VDBP)serves as a key transporter protein responsible for binding and delivering vitamin D and its metabolites to target *** plays a crucial part in the inflammatory reaction following tissue damage and is engaged in actin *** research has shed light on its potential role in various diseases,leading to a growing interest in understanding the implications of VDBP in psychiatric and neurological *** purpose of this review was to provide a summary of the existing understanding regarding the involvement of VDBP in neurological and psychiatric *** examining the intricate interplay between VDBP and these disorders,this review contributes to a deeper understanding of underlying mechanisms and potential therapeutic *** gained from the study of VDBP could pave the way for novel strategies in the diagnosis,prognosis,and treatment of psychiatric and neurological disorders.

关键词： Neurological disorder Polymorphism Psychiatric disorder Structure Vitamin D binding protein

Unconventional Fluorescent and Multi-responsive Polysiloxane:Synthesis,Characterization and Biological Applications

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Chinese Journal of Polymer Science 2024年第5期42卷 579-590,I0006页

作者： Xiao-Di Li Shu-Sheng Li Xu-bao Jiang Xiao-Li Zhu Xiang Zheng kong College of Chemistry and Chemical Engineering University of JinanJinan250022China College of Chemical Engineering Tianjin UniversityTianjin300072China Foshan(Southern China)Institute for New Materials Foshan528200China

Owing to their high significance in fundamental study and diverse applications,stimuli-responsive and fluorescent polymers,particularly those with cluster-triggered emission(CTE)featured by non-conjugated chromophores,have drawn tremendous attention in recent *** this work,fluorescent and multi-responsive polysiloxane(FRPS)was synthesized by hydrolytic condensation polymerization of 3-aminopropyl methyl diethoxysilane(APMS)with 3-(N-isopropyl propionamide)iminopropyl methyl diethoxysilane(APMS-NIP),which was formed in situ through aza-Michael addition between APMS and N-isopropyl *** was not only highly sensitive to temperature,pH and CO_(2) in water,but also showed an enhanced and stimuli-adjustable fluorescence *** effects of monomer feeding,pH and CO_(2) on its lower critical solution temperature and fluorescent property were *** fluorescence emission was ascribed to CTE *** addition,FRPS was shown to be highly potential as physiological indicator for cell imaging,and for controlled release and trace detection of *** study provides therefore a type of stimuli-responsive and fluorescent material for potential applications in biomedical fields,and it is also of great significance for understanding of the fluorescence mechanism of polysiloxane-based stimuli-responsive polymers.

关键词： Polysiloxane Multi-responsiveness Cluster-triggered emission Cell imaging Controlled drug release

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