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Genomes of Meniocus linifolius and Tetracme quadricornis reveal the ancestral karyotype and genomic features of core Brassicaceae

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Plant Communications 2024年第7期5卷 68-89页

作者： Jie liu Shi-Zhao Zhou Yun-Long liu Bin-Yan Zhao dongmei Yu Mi-Cai Zhong Xiao-dong Jiang Wei-Hua Cui Jiu-Xia Zhao Juan Qiu Liang-Min liu Zhen-Hua Guo Hong-Tao Li Dun-Yan Tan Jin-Yong Hu De-Zhu Li CAS Key Laboratory for Plant Diversity and Biogeography of East Asia&yunnan Key Laboratory of Crop Wild Relatives Omics Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Germplasm Bank of Wild Species&yunnan Key Laboratory for Crop Wild Relatives Omics Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China College of Life Sciences Xinjiang Agricultural UniversityÜrümqi 830052China university of Chinese Academy of Sciences Beijing 100049China These authors contributed equally to this article

Brassicaceae represents an important plant family from both a scientific and economic ***,genomic features related to the early diversification of this family have not been fully characterized,especially upon the uplift of the Tibetan Plateau,which was followed by increasing aridity in the Asian interior,intensifying monsoons in Eastern Asia,and significantly fluctuating daily ***,we reveal the genomic architecture that accompanied early Brassicaceae diversification by analyzing two high-quality chromosome-level genomes for Meniocus linifolius(Arabodae;clade D)and Tetracme quadricornis(Hesperodae;clade E),together with genomes representing all major Brassicaceae clades and the basal *** reconstructed an ancestral core Brassicaceae karyotype(CBK)containing 9 pseudochromosomes with 65 conserved syntenic genomic blocks and identified 9702 conserved genes in *** detected pervasive conflicting phylogenomic signals accompanied by widespread ancient hybridization events,which correlate well with the early divergence of core *** identified a successive Brassicaceae-specific expansion of the class I TREHALOSE-6-PHOSPHATE SYNTHASE 1(TPS1)gene family,which encodes enzymes with essential regulatory roles in flowering time and embryo *** TPS1s were mainly randomly amplified,followed by expression *** results provide fresh insights into historical genomic features coupled with Brassicaceae evolution and offer a potential model for broad-scale studies of adaptive radiation under an ever-changing environment.

关键词： Cruciferae genomic features ancient hybridization core Brassicaceae karyotype CBK TREHALOSE-6-PHOSPHATE SYNTHASE 1 genes TPS1s

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大语言模型赋能场景生成和双层优化的多农业园区供电-灌溉-蓄水耦合运行

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高电压技术 2024年第7期50卷 2906-2917,I0007-I0009页

作者：董俊束洪春刘瑞龙文桢张广斌昆明理工大学电力工程学院昆明650500 云南省绿色能源与数字电力量测及控保重点实验室昆明650504 省部共建智能电网故障检测与保护控制协同创新中心(昆明理工大学) 昆明650500

传统农灌负荷的使用具有集中性和无序性,显著加剧电力系统的供需不平衡与运行成本。为提升农业园区运行的经济性和光伏资源的消纳能力,通过刻画农灌负荷特性,结合作物生长用能需求,提出一种基于大语言模型(largelanguagemodel,LLM)场景... 详细信息

传统农灌负荷的使用具有集中性和无序性,显著加剧电力系统的供需不平衡与运行成本。为提升农业园区运行的经济性和光伏资源的消纳能力,通过刻画农灌负荷特性,结合作物生长用能需求,提出一种基于大语言模型(largelanguagemodel,LLM)场景生成和双层优化的多农业园区优化调度模型。该模型利用LLM时序分析能力进行光伏短期发电功率预测,然后通过LLM知识推理能力构建农业用水知识图谱,知识图谱中丰富的语义关系辅助LLM推理和预测,生成更符合实际情况的农业供电-灌溉-蓄水场景。双层优化调度模型在生成场景基础上,以园区经济运行为优化目标,对多农业园区供电-灌溉-蓄水进行耦合优化调度。最后,通过仿真验证,本文所提出的方法显著提升了农业园区电力系统运行稳定性,并有效降低了系统运行成本。

关键词：农灌负荷特性大语言模型知识抽取知识图谱双层优化模型

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Ecology and risks of the global plastisphere as a newly expanding microbial habitat

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The Innovation 2024年第1期5卷 49-58页

作者： Changchao Li Michael RGillings Chao Zhang qinglin Chen dong Zhu Jie Wang Kankan Zhao Qicheng Xu Polly Hangmei Leung Xiangdong Li Jian liu Ling Jin Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityKowloonHong KongChina Environment Research Institute Shandong UniversityQingdao 266237China State Key Laboratory of Marine Pollution City University of Hong KongKowloon TongHong KongChina ARC Centre of Excellence in Synthetic Biology Faculty of Science and EngineeringMacquarie UniversitySydneyNSW 2109Australia Key Laboratory of Urban Environment and Health Ningbo Urban Environment Observation and Research StationInstitute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China Institute of Soil and Water Resources and Environmental Science College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural UniversityNanjing 210095China Department of Health Technology and Informatics and Research Institute for future Food The Hong Kong Polytechnic UniversityKowloonHong KongChina Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China

Plastic offers a new niche for microorganisms,the *** everincreasing emission of plastic waste makes it critical to understand the microbial ecology of the plastisphere and associated ***,we present a global fingerprint of the plastisphere,analyzing samples collected from freshwater,seawater,and terrestrial *** plastisphere assembles a distinct microbial community that has a clearly higher heterogeneity and a more deterministically dominated assembly compared to natural *** coexistence patterns—loose and fragile networks with mostly specialist linkages among microorganisms that are rarely found in natural habitats—are seen in the *** microbiomes generally have a great potential to metabolize organic compounds,which could accelerate carbon *** involved in the nitrogen cycle are also altered in the plastisphere,especially in freshwater plastispheres,where a high abundance of denitrifiers may increase the release of nitrite(aquatic toxicant)and nitrous oxide(greenhouse gas).Enrichment of animal,plant,and human pathogens means that the plastisphere could become an increasingly mobile reservoir of harmful *** findings highlight that if the trajectory of plastic emissions is not reversed,the expanding plastisphere could pose critical planetary health challenges.

关键词： reservoir mostly habitat

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The Comparison of Sugar Components in the Developing Grains of Sweet Corn and Normal Corn

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agricultural Sciences in china 2003年第3期2卷 258-264页

作者： liu Peng, HU Chang-hao, dong Shu-ting, WANG kong-jun and ZHANG Ji-wangCollege of Agronomy , Shandong agricultural university, Taian 271018, P.R.china College of Agronomy Shandong Agricultural University Taian 271018 P. R. China

The sugar components and their dynamic variation in the developing grains of sweet corn(Zea mays L. seccharata Sturt)and normal corn (Zea mays L. indentata Sturt) were compared. There are WSP (water-soluble polysaccharides), sucrose, fructose, glucose, mannitol and sorbitol in both sweet corn and normal corn, but no maltose. Two components with different degrees of polymerization (D. P. N) were detected in the sweet corn; only one of them was detected in the normal corn 20 days after pollination. With the development of grains, the total soluble sugar content(TSS)in sweet corn increased, but in normal corn it decreased. The dynamic variation of WSP, sucrose, glucose, fructose, mannitol and sorbitol in sweet and normal corn grains are different. The contents of sugar components in the sweet corn grains are higher than that in the normal corn. Sweet corn accumulates less starch than normal corn.

关键词： Sweet corn(Zeo mays L. seccharata Sturt) Normal corn(Zea mays L. indentata Sturt),Grain Sugar component HPLC

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温度对黑带食蚜蝇生长发育的影响

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山东农业大学学报（自然科学版） 2004年第2期35卷 221-223页

作者：董坤董艳罗佑珍云南农业大学植物保护学院云南昆明650201

在恒温条件下用烟蚜饲养黑带食蚜蝇 ,得到其各虫态的发育历期 ,用直线回归法求得各虫态的发育起点温度和有效积温 ,同时记录不同温度下各发育阶段的死亡情况及蛹重 ,分析了温度对黑带食蚜蝇生长发育的影响。

关键词：温度黑带食蚜蝇生长发育直线回归法发育起点温度有效积温捕食性天敌

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Genetic Variation of EPAS1 Gene in Tibetan Pigs and Three Low-Altitude Pig Breeds in china

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Journal of Integrative Agriculture 2014年第9期13卷 1990-1998页

作者： dong Kun-zhe KANG Ye YAO Na SHU Guo-tao ZUO qing-qing ZHAO Qian-jun MA Yue-hui Key Laboratory of Farm Animal Genetic Resources and Utilization Ministry of Agriculture/Institute of Animal Science Chinese Academy of Agricultural Sciences College of Animal Science and Technology Yunnan Agricultural University

Endothelial PAS domain protein 1（EPAS1）, also called hypoxia-inducible factor-2, is a key regulatory factor of hypoxic responses and plays an essential role in high-altitude adaptation in mammalian species. In this study, polymorphisms of EPAS1 were detected in 217 individuals from 2 Tibetan pig populations and 3 low-altitude pig breeds by DNA pooling, PCR-SSCP, PCR-RFLP and DNA sequencing methods. A total of 14 synonymous polymorphisms were identified in the coding region. The analysis suggested that SNP1（G963A）, SNP7（C1632T）, SNP10（G1929A） and SNP11（G1947A） showed potential association with high-altitude environment because of their particular variation patterns in Tibetan pigs. Linkage disequilibrium（LD） of these SNPs was analyzed. One common LD block including 5 SNPs clustering in exon 12 was identified in all studied pig populations. Haplotype H1（AGGTC） in LD block was dominant in Tibetan pigs（76.6 and 74.2% in Linzhi（LZ） and Chayu（CY） pigs, respectively） and segregated at higher frequency than that in low-altitude pig breeds（52.3, 58.7 and 56.2% in Wuzhishan（WZS）, Min（M） and Laiwu（LW） pigs, respectively）, indicating that H1 may relate to adaptation to high altitude in Tibetan pigs. These findings raise hope that EPAS1 gene can be a candidate gene that involved in adaptation of high altitude in Tibetan pigs.

关键词： pig EPAS1 gene SNP haplotype nature selection

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Genetic Diversity of Recurrent Selection Populations with Ms2 Gene Assessed by Gliadins in Common Wheat (Triticum aestivum L.)

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agricultural Sciences in china 2010年第5期9卷 615-625页

作者： JIANG Hui, GAO qing-rong, LI Luo-jiang, kong Ling-rang, ZHANG Wei-dong, WU Shi-wen and YANG Ya-li State Key Laboratory of Crop Biology, Department of Agronomy, Shandong agricultural university, Tai’an 271018, P.R.china [a] State Key Laboratory of Crop Biology Department of Agronomy Shandong Agricultural University Tai'an 271018 P.R.China

The male-sterile lines with Ms2 gene were highly evaluated in recurrent selection in wheat （Triticum aestivum L.）. Three populations C6 （population after six cycles of selection）, C7 （population after seven cycles of selection）, and C8 （population after eight cycles of selection） were constructed through recurrent selection with 12 parental materials （P）. Acid polyacrymide gel electrophoresis （A-PAGE） analysis was used to identify gliadin patterns and evaluate the genetic diversity in 12 parents and three populations. A total of 63 bands were identified, of which 17 polymorphic bands and 7 unique bands were present in populations and seven polymorphic bands and four unique bands were present in parents. The number of polymorphic and unique bands decreased gradually from C6 to C8, especially for to- and y-gliadins. The genetic distances in C6, C7, and C8 were calculated. The distributions of genetic distance were different in three recurrent selection populations. From C6 to C8, the genetic distance was 0.2687, 0.2652 and 0.1987, respectively. Statistically significant differences were detected between C7 and C8 with the T value of 37.9718. The result of cluster analysis based on genetic similarity matrix of three populations fitted well to those of principle coordinates analysis （PCoA）. Compared with 12 parents, almost all individuals of three populations are new genotypes. Most of the individuals from C6 and C7 could be divided into two groups, while most individuals of C8 were in one cluster. In conclusion, the results indicated that the genetic diversity was decreased severely according to the information revealed by A-PAGE, although some variations could be created in the recurrent selection. It was necessary to introduce diverse germplasm based on the genetic database of recurrent population to maintain and improve the breeding efficiency in the further program.

关键词： genetic diversity recurrent selection gliadins Ms2 gene Triticum aestivum L

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云南梨地方品种果实表型性状多样性分析

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果树学报 2024年第1期41卷 41-51页

作者：梁艳萍董菲黄文静陈瑶丁仁展李坤明云南省农业科学院园艺作物研究所昆明650205 普洱学院茶叶咖啡学院云南普洱665000

【目的】深入了解云南梨地方品种果实表型性状的变异特点和多样性。【方法】以云南有代表性的61个梨地方品种为试验材料,对果实16个描述型性状和7个数量型性状进行变异类型和多样性分析,利用电子鼻技术检测果实香气并进行主成分分析。... 详细信息

【目的】深入了解云南梨地方品种果实表型性状的变异特点和多样性。【方法】以云南有代表性的61个梨地方品种为试验材料,对果实16个描述型性状和7个数量型性状进行变异类型和多样性分析,利用电子鼻技术检测果实香气并进行主成分分析。【结果】云南梨地方品种果实的16个描述型性状变异类型丰富,其中果实形状和果实成熟期的变异类型最多。各描述型性状的多样性指数(H’)在0.45~1.88之间。19个梨地方品种果实在成熟时果皮分别着鲜红、淡红、粉红、暗红等不同盖色。7个数量型性状变异系数在11.25%~61.79%之间,单果质量的变异系数最高;多样性指数(H’)为1.676~2.080,果实纵径、果柄长度、可溶性固形物含量、果实横径、果形指数的多样性指数均大于2.0。电子鼻技术可以区分供试地方品种,WIW(硫化氢类)、W5S(氮氧化物)、W2W(芳香成分和有机硫化物)等3个传感器在模式识别中起主要作用。【结论】云南梨地方品种果实描述型性状变异类型丰富,数量型性状多样性指数较高。筛选出会泽火瓢梨等7个果皮着鲜红色的品种可作为红色梨选育的优异资源。电子鼻技术可以区分61个云南梨地方品种,可用作地方品种鉴定的辅助手段。

关键词：梨云南地方品种果实性状多样性电子鼻

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鹤山地区健康和失管茶园土壤真菌群落结构差异及其驱动因子分析

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微生物学报 2024年第5期64卷 1417-1435页

作者：王星董义杰冯广达姚青柳陈坚朱红惠昆明理工大学生命科学与技术学院云南昆明650500 广东省科学院微生物研究所、华南应用微生物国家重点实验室、农业农村部农业微生物组学与精准应用重点实验室、农业农村部农业微生物组学重点实验室、广东省菌种保藏与应用重点实验室广东广州510070 华南农业大学园艺学院、广东省荔枝工程技术研究中心广东广州510642

【目的】以鹤山红茶产地生态系统为研究对象,探究健康和失管茶园中土壤真菌群落结构的差异以及土壤理化性质对真菌群落结构的影响。【方法】在鹤山地区健康茶园和管理不善的失管茶园中采集了49份根围土壤样品,采用高通量测序技术分析茶... 详细信息

【目的】以鹤山红茶产地生态系统为研究对象,探究健康和失管茶园中土壤真菌群落结构的差异以及土壤理化性质对真菌群落结构的影响。【方法】在鹤山地区健康茶园和管理不善的失管茶园中采集了49份根围土壤样品,采用高通量测序技术分析茶树根围土壤真菌的群落组成;利用冗余分析研究土壤理化性质对真菌群落结构的影响;进一步通过Wilcoxon秩和检验法分析健康和失管茶园中差异的真菌类群;通过fuNGuild软件对土壤真菌的功能类群进行预测。【结果】研究发现担子菌门、被孢霉门和子囊菌门是茶树根围土壤的优势门;失管茶园中担子菌门的相对丰度显著升高,而被孢霉门则显著降低;根围土壤真菌的丰富度指数、Chao1指数和ACE指数显著低于健康茶园。研究发现总氮、总磷、有效磷、有机质和水解性氮是真菌β多样性差异的主要环境驱动因子;pH、总磷、交换性镁、交换性钙、有效磷和有效钾等与真菌类群间存在显著的相关性。健康和失管茶园中共有的核心操作分类单元(operational taxonomic units,OTUs)是10个,失管茶园中核心类群的相对丰度降低,而中间类群和稀有类群的相对丰度则增加。健康茶园根围土壤核心OTUs中出现了茶轮斑病菌茶拟盘多毛孢和国槐根腐病菌角化可塑镰孢菌(fusarium keratoplasticum),失管茶园根围土壤核心OTUs中生防菌螺旋木霉(Trichoderma spirale)、深绿木霉(***)的丰度显著较高。失管茶园中病原营养型、病原-腐生-共生过渡型和共生营养型真菌的相对丰度明显增加;而腐生-共生过渡型的真菌则显著降低。【结论】本研究揭示了鹤山地区茶园管理方式与真菌群落结构和土壤理化性质间的关系,为鹤山红茶的病害防治及生防菌筛选指明了方向。

关键词：茶树鹤山地区根围土壤真菌土壤元素含量相关性

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基于改进YOLOv7的苹果表面缺陷轻量化检测算法

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河南农业科学 2024年第3期53卷 141-150页

作者：李大华孔舒李栋于晓天津理工大学电气工程与自动化学院天津300384 天津市新能源电力变换传输与智能控制重点实验室天津300384 天津市复杂系统控制理论及应用重点实验室天津300384

针对如何提高苹果表面缺陷的检测速度和精度,解决模型内存占比大的问题,提出一种基于改进YOLOv7的苹果表面缺陷轻量化检测算法。首先引入GhostNetV2作为YOLOv7网络的backbone,有效降低了模型复杂度,提高了检测速度。并引入SimAM无参注... 详细信息

针对如何提高苹果表面缺陷的检测速度和精度,解决模型内存占比大的问题,提出一种基于改进YOLOv7的苹果表面缺陷轻量化检测算法。首先引入GhostNetV2作为YOLOv7网络的backbone,有效降低了模型复杂度,提高了检测速度。并引入SimAM无参注意力机制,以强化不同深度的特征信息。使用双向加权特征金字塔结构BiFPN进行加权特征融合,进一步提升苹果表面缺陷的检测精度。最后采用ECIOU损失函数来计算边界框损失,进一步提高模型收敛速度和整体性能。结果表明,改进YOLOv7模型在苹果表面缺陷检测上mAP@0.5较原YOLOv7网络提高2.0百分点,准确率和召回率也分别提升了1.7、3.9百分点,模型减小20.8 MB,速度提升36.43帧/s。其综合性能也优于SSD、CenterNet等主流算法,可实现对苹果表面缺陷的快速准确诊断。

关键词：苹果表面缺陷 YOLOv7 GhostNetV2 注意力机制 BiFPN ECIOU

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