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Harnessing naturally occurring mutations for T-cell therapy:a potential new avenue to enhance treatment efficacy

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Signal Transduction and Targeted Therapy 2024年第5期9卷 1799-1801页

作者： Michael Hiltensperger Jürgen Ruland kilian Schober Mikrobiologisches Institut-Klinische Mikrobiologie Immunologie und HygieneFriedrich-Alexander-Universität Erlangen-NürnbergErlangenGermany Institute of Clinical Chemistry and Pathobiochemistry School of MedicineTechnical University of MunichMunichGermany TranslaTUM Center for Translational Cancer ResearchTechnical University of MunichMunichGermany German Cancer Consortium(DKTK) HeidelbergGermany German Center for Infection Research(DZIF) partner site MunichMunichGermany FAU Profile Center Immunomedicine Friedrich-Alexander-Universität Erlangen-NürnbergErlangenGermany

In a recent study published in Nature,Garcia et *** a sophisticated approach to identify fitness-enhancing mutations for T cells that was inspired by cancer evolution.1 The identified CARD11-PIK3R3 gene fusion enhanced tumor rejection and persistence of engineered T cells in multiple tumor models and might have the potential to improve efficacy of adoptive T-cell therapies in cancer patients.

关键词： cancer potential

Identification of abnormal tribological regimes using a microphone and semi-supervised machine-learning algorithm

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Friction 2022年第4期10卷 583-596页

作者： Vigneashwara PANDIYAN Josef PROST Georg VORLAUFER Markus VARGA kilian WASMER Laboratory for Advanced Materials Processing(LAMP) Swiss Federal Laboratories for Materials Science and Technology(Empa)Thun CH-3602Switzerland AC2T research GmbH Viktor-Kaplan-Straße 2/CWiener Neustadt 2700Austria

Functional surfaces in relative contact and motion are prone to wear and tear,resulting in loss of efficiency and performance of the workpieces/*** occurs in the form of adhesion,abrasion,scuffing,galling,and scoring between ***,the rate of the wear phenomenon depends primarily on the physical properties and the surrounding *** the integrity of surfaces by offline inspections leads to significant wasted machine time.A potential alternate option to offline inspection currently practiced in industries is the analysis of sensors signatures capable of capturing the wear state and correlating it with the wear phenomenon,followed by in situ classification using a state-of-the-art machine learning(ML)*** this technique is better than offline inspection,it possesses inherent disadvantages for training the ML ***,supervised training of ML models requires the datasets considered for the classification to be of equal weightage to avoid *** collection of such a dataset is very cumbersome and expensive in practice,as in real industrial applications,the malfunction period is minimal compared to normal ***,classification models would not classify new wear phenomena from the normal regime if they are *** a promising alternative,in this work,we propose a methodology able to differentiate the abnormal regimes,i.e.,wear phenomenon regimes,from the normal *** is carried out by familiarizing the ML algorithms only with the distribution of the acoustic emission(AE)signals captured using a microphone related to the normal *** a result,the ML algorithms would be able to detect whether some overlaps exist with the learnt distributions when a new,unseen signal *** achieve this goal,a generative convolutional neural network(CNN)architecture based on variational auto encoder(VAE)is built and *** the validation procedure of the proposed CNN architectures,we were

关键词： surface integrity acoustic emission auto encoders condition monitoring wear prediction

A Novel Two Domain-Fusion Protein in Cyanobacteria with Similarity to the CAB/ELIP/HLIP Superfamily： Evolutionary Implications and Regulation

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Molecular Plant 2008年第1期1卷 155-166页

作者： Oliver kilian Anne Soisig Steunou Arthur R. Grossman Devaki Bhaya Department of Plant Biology The Carnegie Institution Stanford CA 94305 USA

Vascular plants contain abundant, light-harvesting complexes in the thylakoid membrane that are non-covalently associated with chlorophylls and carotenoids. These light-harvesting chlorophyll a/b binding （LHC） proteins are members of an extended CAB/ELIP/HLIP superfamily of distantly related polypeptides, which have between one and four transmembrane helices （TMH）. This superfamUy includes the single TMH, high-light-inducible proteins （Hlips）, found in cyanobacteria that are induced by various stress conditions, including high light, and are considered ancestral to the LHC proteins. The roles of, and evolutionary relationships between, these superfamily members are of particular interest, since they function in both light harvesting and photoprotection and may have evolved through tandem gene duplication and fusion events. We have investigated the Hlips （hli gene family） in the thermophilic unicellular cyanobacterium Synechococcus OS-B＇. The five hli genes present on the genome of Synechococcus OS-B＂ are relatively similar, but transcript analyses indicate that there are different patterns of transcript accumulation when the cells are exposed to various growth conditions, suggesting that different Hlips may have specific functions. Hlip5 has an additional TMH at the N-terminus as a result of a novel fusion event. This additional TMH is very similar to a conserved hypothetical, single membrane-spanning polypeptide present in most cyanobacteria. The evolutionary significance of these results is discussed.

关键词： fusion proteins Cyanobacteria N-Terminus stress conditions Large Hadron Collider Growth conditions photostability Regulation (biology) Thylakoid Membrane Tracheobionta gene duplication Analogy superfamily Chlorophyll Evolutionary

Longs short-term based semi-supervised memory encoder-decoder for early prediction of failures in self-lubricating bearings

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Friction 2023年第1期11卷 109-124页

作者： Vigneashwara PANDIYAN Mehdi AKEDDAR Josef PROST Georg VORLAUFER Markus VARGA kilian WASMER Laboratory for Advanced Materials Processing(LAMP) Swiss Federal Laboratories for Materials Science and Technology(Empa)Thun CH-3602Switzerland AC2T research GmbH Wiener Neustadt 2700Austria

The existing knowledge regarding the interfacial forces,lubrication,and wear of bearings in real-world operation has significantly improved their designs over time,allowing for prolonged service *** a result,self-lubricating bearings have become a viable alternative to traditional bearing designs in industrial ***,wear mechanisms are still inevitable and occur progressively in self-lubricating bearings,as characterized by the loss of the lubrication film and ***,monitoring the stages of the wear states in these components will help to impart the necessary countermeasures to reduce the machine maintenance *** article proposes a methodology for using a long short-term memory(LSTM)-based encoder-decoder architecture on interfacial force signatures to detect abnormal regimes,aiming to provide early predictions of failure in self-lubricating sliding contacts even before they *** sliding experiments were performed using a self-lubricating bronze bushing and steel shaft journal in a custom-built transversally oscillating tribometer *** force signatures corresponding to each cycle of the reciprocating sliding motion in the normal regime were used as inputs to train the encoder-decoder architecture,so as to reconstruct any new signal of the normal regime with the minimum *** this semi-supervised training exercise,the force signatures corresponding to the abnormal regime could be differentiated from the normal regime,as their reconstruction errors would be very *** the validation procedure for the proposed LSTM-based encoder-decoder model,the model predicted the force signals corresponding to the normal and abnormal regimes with an accuracy of 97%.In addition,a visualization of the reconstruction error across the entire force signature showed noticeable patterns in the reconstruction error when temporally decoded before the actual critical failure point,making it possible to be used for early predic

关键词： predictive maintenance in-situ sensing long short-term memory(LSTM) encoder-decoder wear monitoring tribology

Evaluation of different crosslinking methods in altering the properties of extrusion-printed chitosan-basedmulti-material hydrogel composites

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Bio-Design and Manufacturing 2023年第2期6卷 150-173页

作者： Suihong Liu Haiguang Zhang Tilman Ahlfeld David kilian Yakui Liu Michael Gelinsky Qingxi Hu Centre for Translational Bone Joint and Soft Tissue ResearchFaculty of Medicine and University Hospital Carl Gustav CarusTechnische Universität Dresden01307 DresdenGermany Rapid Manufacturing Engineering Center School of Mechatronic Engineering and AutomationShanghai UniversityShanghai 200444China Shanghai Key Laboratory of Intelligent Manufacturing and Robotics School of Mechatronic Engineering and AutomationShanghai UniversityShanghai 200444China National Demonstration Center for Experimental Engineering Training Education Shanghai UniversityShanghai 200444China

Three-dimensional printing technologies exhibit tremendous potential in the advancing fields of tissue engineering and regenerative medicine due to the precise spatial control over depositing the *** their widespread utilization and numerous advantages,the development of suitable novel biomaterials for extrusion-based 3D printing of scaffolds that support cell attachment,proliferation,and vascularization remains a ***-material composite hydrogels present incredible potential in this ***,in this work,a multi-material composite hydrogel with a promising formulation of chitosan/gelatin functionalized with egg white was developed,which provides good printability and shape *** addition,a series of comparative analyses of different crosslinking agents and processes based on tripolyphosphate(TPP),genipin(GP),and glutaraldehyde(GTA)were investigated and compared to select the ideal crosslinking strategy to enhance the physicochemical and biological properties of the fabricated *** of the results indicate that the composite hydrogel and the resulting scaffolds utilizing TPP crosslinking have great potential in tissue engineering,especially for supporting neo-vessel growth into the scaffold and promoting angiogenesis within engineered tissues.

关键词： Multi-material composite hydrogel Crosslinking mechanism Chitosan Gelatin Egg white 3D printing

Self-aligned single-electrode actuation of tangential and wineglass modes using PMN-PT

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Microsystems & Nanoengineering 2023年第3期9卷 43-49页

作者： Ozan Erturk kilian Shambaugh Ha-Seong Park Sang-Goo Lee Sunil A.Bhave OxideMEMS Lab Purdue UniversityWest LafayetteINUSA Polytec Inc. IrvineCAUSA iBULe Photonics Company Ltd. Incheon 21999South Korea

Considering the evolution of rotation sensing and timing applications realized in micro-electro-mechanical systems(MEMS),flexural mode resonant shapes are outperformed by bulk acoustic wave(BAW)counterparts by achieving higher frequencies with both electrostatic and piezoelectric *** the 1-30 MHz range,which hosts BAW gyroscopes and timing references,piezoelectric and electrostatic MEMS have similar transduction ***,when designed intelligently,electrostatic transduction allows self-alignment between electrodes and the resonator for various BAW modes,misalignment is inevitable regarding piezoelectric transduction of BAW modes that require electrode *** this paper transverse piezoelectric actuation of[011]oriented single crystal lead magnesium niobate-lead titanate(PMN-PT)thin film disks are shown to excite the tangential mode and family of elliptical compound resonant modes,utilizing a self-aligned and unpatterned electrode that spans the entire disk *** resonant mode coupling is achieved by employing a unique property of[011]PMN-PT,where the in-plane piezoelectric coefficients have opposite *** 1-port disk transducers,RF reflection measurements are performed that demonstrate the compound mode family shapes in the 1-30 MHz *** verification of mode transduction is achieved using in-plane displacement measurements with Polytec’s laser Doppler vibrometer(LDV).While the tangential mode achieves a 40°/s dithering rate at 335 kHz resonant frequency,the n=2 wine-glass mode achieves 11.46 nm tip displacement at 8.42 MHz resonant frequency on a radius of 60μm disk resonator in air.A single electrode resonator that can excite both tangential and wine-glass modes with such metrics lays the foundation for a BAW MEMS gyroscope with a built-in primary calibration stage.

关键词： resonator electrode piezoelectric

Mojiangia oreophila(Crepidinae,Cichorieae,Asteraceae),a new species and genus from Mojiang County,SW Yunnan,China,and putative successor of the maternal Faberia ancestor

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Plant Diversity 2022年第1期44卷 83-93页

作者： Zhi-Jian Yin Ze-Huan Wang Norbert kilian Ying Liu Hua Peng Ming-Xu Zhao China Forest Exploration and Design Institute in Kunming Kunming650200YunnanChina Department of Medicinal Plants and Cultivation College of PharmacyGuizhou University of Traditional Chinese MedicineGuiyang550025China Botanischer Garten und Botanisches Museum Berlin Freie Universitat BerlinKonigin-Luise-Str.6-814195BerlinGermany CAS State Key Laboratory ofBiocontrol and Guangdong Key Laboratory of Plant Resources School of Life SciencesSun Yat-sen UniversityGuangzhou510275China Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming650201China

A single small population of chasmophytic plants is described as Mojiangia oreophila,a monotypic genus in the subtribe Crepidinae,characterised by a unique combination of morphological features,in particular densely long-papillose homomorphic achenes with five main ribs each accompanied by two secondary ribs,coarse brownish pappus bristles,moderately many-flowered capitula,a small involucre with numerous outer phyllaries,perennial rosette herb growth and brown-woolly caudex and leaf *** phylogenetic analysis detected that in the nrlTS phylogeny *** forms a clade of its own in the Crepidinae;in the plastid DNA phylogeny it is nested in the clade formed by the hybrid-ogenous genus Faberia,the maternal ancestor of which comes from the Crepidinae and the paternal ancestor from the Lactucinae,where Faberia is placed in nrlTS *** shares several morphological features with Faberia and also shares the expected chromosome number of 2n=16 with its hitherto unknown maternal *** may therefore be a successor of the maternal ancestor of ***,cytonuclear discordance is to be assumed in Mojiangia,caused by chloroplast capture as a result of hybridisation and introgression with Faberia.

关键词： Asteraceae Crepidinae Phylogeny Taxonomy Reticulate evolution Cytonuclear discordance

GaAs/AlGaAs heterostructure nanowires studied by cathodoluminescence

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Nano Research 2014年第4期7卷 473-490页

作者： Jessica Bolinsson Martin Ek Johanna Tragardh kilian Mergenthaler Daniel Jacobsson Mats-Erik Pistol Lars Samuelson Anders Gustafsson Solid State Physics and the Nanometer Structure Consortium Lund University Box 118 SE-221 00 Lund Sweden Polymer and Materials Chemistry Lund University Box 124 SE-221 00 Lund Sweden

In this report we explore the structural and optical properties of GaAs/A1GaAs heterostructure nanowires grown by metalorganic vapour phase epitaxy using gold seed-particles. The optical studies were done by low-temperature cathodo- luminescence （CL） in a scanning electron microscope （SEM）. We perform a systematic investigation of how the nanowire growth-temperature affects the total photon emission, and variations in the emission energy and intensity along the length of the nanowires. The morphology and crystal structures of the nanowires were investigated using SEM and transmission electron microscopy （TEM）. In order to correlate specific photon emission characteristics with variations in the nanowire crystal structure directly, TEM and spatially resolved CL measurements were performed on the same individual nanowires. We found that the main emission energy was located at around 1.48 eV, and that the emission intensity was greatly enhanced when increasing the GaAs nanowire core growth temperature. The data strongly suggests that this emission energy is related to rotational twins in the GaAs nanowire core. Our measurements also show that radial overgrowth by GaAs on the GaAs nanowire core can have a deteriorating effect on the optical quality of the nanowires. Finally, we conclude that an in situ pre-growth annealing step at a sufficiently high temperature significantly improves the optical quality of the nanowires.

关键词： GaAs/AlGaAs core shellnanowires metalorganic vapourphase epitaxy (MOVPE) cathodoluminescence twin defects transmission electronmicroscopy

Dysregulated expression and distribution of Kif5αin neurites of wobbler motor neurons

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Neural Regeneration Research 2023年第1期18卷 150-154页

作者： kilian Kürten Anne-Christin Gude Aimo Samuel Christian Epplen Jan Stein Carsten Theiss Veronika Matschke Department of Cytology Institute of AnatomyMedical FacultyRuhr University BochumBochumGermany

Impaired axonal transport has been observed in patients with amyotrophic lateral sclerosis(ALS)and in animal models,suggesting that transport proteins likely play a critical role in the pathological mechanism of *** of Kinesin-family-member 5α(Kif5α),a neuron-specific isoform of heavy chain kinesin family,has been described in several neurological disorders,in humans and animal models,including *** this study,we determined Kif5αexpression by gene sequencing,quantitative reverse transcription-polymerase chain reaction,and western blot assay in the cervical spinal cord of wobbler mice and immunofluorescence staining in dissociated cultures of the ventral ***,we observed the distribution of Kif5αand mitochondria along motor neuronal branches by confocal *** results showed that Kif5αexpression was greatly dysregulated in wobbler mice,which resulted in altered distribution of Kif5αalong motor neuronal branches with an abnormal mitochondrial ***,our results indicate that dysregulation of Kif5 and therefore abnormal transport in motor neuronal branches in this ALS model could be causative for several pathological findings at the cellular level,like misallocation of cytoskeletal proteins or organelles like mitochondria.

关键词： amyotrophic lateral sclerosis cell culture kinesin mitochondria neurodegeneration spinal cord transport