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三氯乙烯药疹样皮炎患者肿瘤坏死因子α-857C/T基因多态性研究

首届世界华人自由基生物学与自由基医学学术大会暨第五届海峡两岸三地自由基生物学与自由基医学研讨会第八届全国自由基生物学与自由基医学大会

作者： ke yue-bin department of genetic toxicology,shenzhen center for disease control and prevention,shenzhen 518055,p.r.china

8-oxo-7,8-dihydroguanine(8-oxoG) is a major pre-mutagenic lesion generated from reactive oxygen *** gene and Mthl gene play a major role in maintaining genetic integrity by removing *** investigate the relationship in... 详细信息

关键词： OGGI MTHI 8-oxoG

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中国职业医学 2006年第5期33卷 349-351页

作者：庾蕾庄志雄叶小明黄海雄杨淋清深圳市疾病预防控制中心食品(毒理)科广东深圳518020

目的从肿瘤坏死因子α(TNFα)基因-857位点多态性角度探讨三氯乙烯药疹样皮炎易感的遗传背景。方法采用TaqManMGB探针,建立TNFα-857C/T位点实时定量聚合酶链反应分型方法。用该方法对三氯乙烯药疹样皮炎组(47例)及对照组(53例)进行-857... 详细信息

目的从肿瘤坏死因子α(TNFα)基因-857位点多态性角度探讨三氯乙烯药疹样皮炎易感的遗传背景。方法采用TaqManMGB探针,建立TNFα-857C/T位点实时定量聚合酶链反应分型方法。用该方法对三氯乙烯药疹样皮炎组(47例)及对照组(53例)进行-857nt(C/T)位点分型,比较病例组与对照组之间各基因型及等位基因频率是否有差异。结果TNFα-857C/T多态的基因型及等位基因频率在三氯乙烯药疹样皮炎组及对照组差异无显著性(p>0.05)。结论TNFα-857C/T位点多态可能不是中国人群三氯乙烯药疹样皮炎的风险因子。

关键词：三氯乙烯药疹样皮炎肿瘤坏死因子α 单核苷酸多态性

Synthesis of haptens and production of antibodies to bisphenol A

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Frontiers of Agricultural Science and Engineering 2017年第3期4卷 366-372页

作者： Xiya ZHANG Xiaoyun DONG Sijun ZHAO yuebin ke Kai WEN Suxia ZHANG Zhanhui WANG Jianzhong SHEN Beijing Advanced Innovation center for Food Nutrition and Human Health/College of Veterinary Medicine China Agricultural University Beijing key Laboratory of Detection Technology for Animal-Derived Food Safety department for Safety Supervision of Animal products China Animal Health and Epidemiology Center department of genetic toxicology Shenzhen Center for Disease Control and Prevention Beijing Laboratory for Food Quality and Safety/National reference Laboratory for Veterinary Drug residues

Three immunizing haptens of bisphenol A(BpA), including two new haptens, were used to produce highly sensitive and specific polyclonal antibodies. The spacer arms of haptens for coupling to the protein carrier were located at different positions in BpA, and different length spacer arms were tested. Highly sensitive polyclonal antibodies were obtained and characterized using indirect competitive enzyme-linked immunosorbent assay(ic ELISA). Under optimized conditions, the half maximal inhibitory concentration(IC50) value of the best polyclonal antibody was 2.1 mg·L^(-1), based on coating heterogeneous antigens, and this optimal polyclonal antibody was highly sensitive toward BpA and displayed negligible crossreactivity with bisphenol B and bisphenol E. A sensitive ic ELISA method utilizing the polyclonal antibody was developed for the determination of BpA in milk. In spiked samples(5, 10 and 20 mg·L^(-1)), the recovery ranged from 80% to 102% with a coefficient of variation(CV) value below 15.8%. The limit of detection of ic ELISA was1.95 mg·L^(-1). These results indicate that the ic ELISA method is suitable for the detection of BpA in milk.

关键词： bisphenol A cross-reactivity hapten indirect competitive ELISA polyclonal antibody

CDH13 genetic polymorphisms, Adiponectin and Ischemic Stroke： a Chinese Family-based Sib-pair Study

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Biomedical and Environmental Sciences 2017年第1期30卷 35-43页

作者： CHEN Li SUN ke Xin JUAN Juan FANG Kai LIU Kuo WANG Xue Yin WANG Ling YANG Chao LIU Xiao Qiang LI Jing TANG Xun WU Yi Qun QIN Xue Ying WU Tao CHEN Da Fang HU Yong Hua department of Epidemiology and Biostatistics Peking University Health Science Center Beijing 100191 China Beijing center for disease prevention and control Beijing 100013 China department of Epidemiology & Biostatistics Capital Medical University Beijing 100069 China pingshan New District center for disease control and prevention Shenzhen 518118 Guangdong China Shilou Township Hospital Beijing 102422 China

Objective To understand the relationships between CDH13（T-cadherin） genetic polymorphisms, adiponectin levels and ischemic stroke, and possible interactions between CDH13 polymorphisms and other risk factors. Methods We recruited 342 Chinese ischemic stroke sib pairs. We genotyped rs4783244 and rs7193788 on CDH13 using time-of-flight mass spectrometry genotyping technology and measured total and high-molecular weight（HMW） adiponectin levels. We investigated associations between SNps and ischemic stroke, and interactions between SNps and other risk factors using multi-level mixed-effects regression model. results In individuals without ischemic stroke, CDH13 rs4783244 was associated with total adiponectin levels（per T： Coef =-0.257, p = 0.001）. CDH13 rs7193788 was associated with total adiponectin levels（per A： Coef =-0.221, p = 0.001） and HMW adiponectin levels（per A： Coef =-0.163, p = 0.003）. rs7193788 was significantly associated with ischemic stroke（GA/AA vs. GG： Or = 1.55, 95% CI： 1.07 to 2.24, p = 0.020） after Bonferroni correction（α = 0.025）. There was an interaction between rs7193788 and diabetes（p = 0.036）. Compared to diabetes-free individuals with rs7193788 GG genotype, diabetes patients with rs7193788 GA/AA genotypes had higher risks for ischemic stroke（Or = 2.64, 95% CI： 1.58-4.40, p 〈 0.001）. Conclusion CDH13 genetic polymorphisms are associated with adiponectin levels and ischemic stroke. An interaction is found between CDH13 SNp and diabetes for ischemic stroke.

关键词： Family correction cadherin SNps genotype transformed preserving false trained burden

溶酶体酸脂酶基因变异与冠心病风险的关联研究

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中国分子心脏病学杂志 2020年第4期20卷 3497-3500页

作者：徐延路柯晓惠汝太李莉郑欣馨中国医学科学院北京协和医学院国家心血管病中心阜外医院心内科北京市100037 中国医学科学院阜外医院深圳医院心血管内科深圳市518057 黑龙江省鹤岗市人民医院心血管内科鹤岗市510120

目的在中国人群中验证溶酶体酸脂酶(LAL)基因变异性与冠心病遗传易感性。方法对经冠状动脉造影确诊的568个冠心病病例和430个正常对照的人群,采用pCr-LDr法对rs1412444进行基因分型。按照显性模式、隐性模式和加性模式进行关联分析,探讨... 详细信息

目的在中国人群中验证溶酶体酸脂酶(LAL)基因变异性与冠心病遗传易感性。方法对经冠状动脉造影确诊的568个冠心病病例和430个正常对照的人群,采用pCr-LDr法对rs1412444进行基因分型。按照显性模式、隐性模式和加性模式进行关联分析,探讨rs1412444变异与冠心病风险的关联。结果rs1412444基因型变异与冠心病的易感性密切相关。与携带G基因型相比,携带A等位基因能够显著增加冠心病的发病风险。经校正传统危险因素后,LIpA-rs1412444 A等位基因携带者与野生型G等位基因携带者相比,仍显示冠心病易感性增加(显性模式:Or=1.37,95%CI:1.01-1.88,p=0.046;加性模式:Or=1.30,95%CI:1.02-1.65,p=0.032)。结论LIpA基因多态性位点rs1412444可能是预测冠心病发病的新遗传标记,溶酶体酸脂酶可能在冠心病的发病中起到重要的调节作用。

关键词：溶酶体酸脂酶基因单核苷酸多态性脂质代谢冠心病