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SIRT1基因沉默对DLBCL细胞放射敏感性影响

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中华放射肿瘤学杂志 2017年第6期26卷 687-690页

作者：康议心高社干郭艳珍姚俊张治业高小会张殿宝郭双双张路霖河南科技大学第一附属医院肿瘤科洛阳471003

目的探究SIRT1基因沉默对DLBCL放射敏感性影响。方法利用免疫组织化学观察SIRT1在DLBCL组织中的蛋白表达情况,蛋白印迹法检测SIRT1在DLBCL细胞系OCI-Ly3、SU-DHL-2、SU-DHL-4及人正常B细胞永生化细胞系***中的表达情况。利用Lipofecta... 详细信息

目的探究SIRT1基因沉默对DLBCL放射敏感性影响。方法利用免疫组织化学观察SIRT1在DLBCL组织中的蛋白表达情况,蛋白印迹法检测SIRT1在DLBCL细胞系OCI-Ly3、SU-DHL-2、SU-DHL-4及人正常B细胞永生化细胞系***中的表达情况。利用Lipofectamine 2000转染试剂盒将si-SIRT1及si-NC转染到SU-DHL-4细胞中并检测转染后SIRT1的表达;MTT法和克隆形成实验检测经放射处理后细胞放射敏感性的变化。成组t检验差异或单因素方差分析。结果 SIRT1在DLBCL组织中的阳性率为72.6%（103/140）,明显高于SIRT1在RLH中的阳性率（26.5%,8/25）（P=0.001）;SIRT1在DLBCL细胞系中的表达水平显著高于人正常B细胞永生化细胞系***细胞（P=0.020）;SIRT1基因沉默后,SIRT1在SU-DHL-4细胞中的表达量明显降低（P=0.008）。放射处理后,SIRT1基因沉默组的细胞增殖率和克隆形成率显著低于对照组（P=0.030）。结论 SIRT1基因沉默明显提高了DLBCL细胞的放射敏感性,为DLBCL细胞的治疗提供了一个新的靶标。

关键词： SIRT1基因基因敲除弥漫性大B淋巴瘤细胞放射敏感性

湖南省鸟类分布新记录——白肩雕、峨眉鹟莺、淡尾鹟莺

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湖南林业科技 2023年第6期50卷 83-90页

作者：康祖杰康艺馨舒兆恩贺春容唐甲文哲伊剑峰曾超辉杨道德湖南壶瓶山国家级自然保护区管理局湖南石门415319 西南林业大学生物多样性保护学院云南昆明650224 生态环境部南京环境科学研究所江苏南京210042 中南林业科技大学野生动植物保护研究所湖南长沙410004 湖南文理学院湖南常德415000 动物学湖南省高校重点实验室湖南常德415000

为了解湖南省壶瓶山国家级自然保护区鸟类多样性及其种群动态,于2010年1月—2022年12月,采用样线法和固定样点法对该区鸟类资源进行了观测,观察并拍摄到1种鹰形目和2种雀形目鸟,经鉴定分别为白肩雕、峨眉鹟莺、淡尾鹟莺。经查阅相关文献... 详细信息

为了解湖南省壶瓶山国家级自然保护区鸟类多样性及其种群动态,于2010年1月—2022年12月,采用样线法和固定样点法对该区鸟类资源进行了观测,观察并拍摄到1种鹰形目和2种雀形目鸟,经鉴定分别为白肩雕、峨眉鹟莺、淡尾鹟莺。经查阅相关文献,确定这3种鸟均为湖南省鸟类分布新记录种。新记录种的发现丰富了湖南省鸟类物种多样性,对湖南省及周边动物地理区系关系的研究具有一定的参考价值。

关键词：鸟类分布新记录白肩雕峨眉鹟莺淡尾鹟莺

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山东省微山县郗山稀土矿床成矿年代学新进展

地质科技通报 2024年第1期43卷 51-62页

作者：张鹏刘珊珊兰君李衣鑫邢楠刘敬杰刘袁坤康志强杨锋山东省地质矿产勘查开发局第五地质大队山东泰安271000 桂林理工大学地球科学学院广西桂林541004 桂林理工大学有色金属矿产勘查与资源高效利用省部共建协同创新中心广西桂林541004

山东省微山县郗山稀土矿床位于华北板块东南缘,是一个以轻稀土为主的岩浆热液型稀土矿床,目前为我国第三大轻稀土矿床,对其成矿年龄还存在争议。为此,以郗山稀土矿最新勘探钻孔矿石样品为研究对象,通过野外调查、矿物学和年代学研究,采... 详细信息

山东省微山县郗山稀土矿床位于华北板块东南缘,是一个以轻稀土为主的岩浆热液型稀土矿床,目前为我国第三大轻稀土矿床,对其成矿年龄还存在争议。为此,以郗山稀土矿最新勘探钻孔矿石样品为研究对象,通过野外调查、矿物学和年代学研究,采用氟碳铈矿Th-Pb同位素定年精确厘定了郗山稀土矿的成矿年代。5件氟碳铈矿Th-Pb同位素定年结果表明,郗山稀土矿床成矿时代可分为2期:早期为(125.5±0.5)~(122.2±0.5)Ma,晚期为(112.3±0.6)Ma,其中早期年龄与前人得出的矿床成矿年龄基本一致,而晚期成矿年龄则是首次报道,表明郗山稀土矿床可能存在2期成矿活动,值得关注并开展进一步工作。

关键词：郗山稀土矿床 Th-Pb同位素定年氟碳铈矿微山县山东省

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针刺治疗单纯性肥胖症48例

中医外治杂志 2005年第4期14卷 32-32页

作者：康轶鑫山东省淄博市临淄区人民医院针灸科山东淄博255400

单纯性肥胖症主要是指没有明显的神经系统和内分泌系统疾病,而是由于机体内热量的摄入大于消耗,造成脂肪在体内堆积过多,导致体重超常的病证.且容易并发多种疾病,如Ⅱ型糖尿病、高脂血症、高血压病、冠心病等,严重危害人类的健康.笔者... 详细信息

单纯性肥胖症主要是指没有明显的神经系统和内分泌系统疾病,而是由于机体内热量的摄入大于消耗,造成脂肪在体内堆积过多,导致体重超常的病证.且容易并发多种疾病,如Ⅱ型糖尿病、高脂血症、高血压病、冠心病等,严重危害人类的健康.笔者采用针刺治疗本病48例,疗效满意,现报道如下.

关键词：单纯性肥胖症针刺疗法临床疗效

QSPR modeling of azeotropic temperatures and compositions for binary azeotropes containing lower alcohols using a genetic function approximation

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Chinese Journal of Chemical Engineering 2019年第4期27卷 835-844页

作者： yixin Ma kang Ma Huixin Wang Xueli Geng Jun Gao Zhaoyou Zhu Yinglong Wang College of Chemical and Environmental Engineering Shandong University of Science and Technology Qingdao 266590 China College of Chemical Engineering Qingdao University of Science and Technology Qingdao 266042 China National Registration Center for Chemicals SINOPEC Research Institute of Safety Engineering Qingdao 266071 China

Binary azeotropes, which contain two chemicals with a relative volatility of 1, are very common in the chemical industry. Understanding azeotropes is essential for effectively separating binary azeotropes containing lower alcohols. Experimental techniques and ab initio approaches can produce accurate results;however, these two processes are time consuming and labor intensive. Although thermodynamic equations such as UNIFAC are widely used, experimental values are required, and it is difficult to choose the best groups to represent a complex system. Because of their high efficiency and fast calculation speed, quantitative structure–property relationship(QSPR) tools were used in this work to predict the azeotropic temperatures and compositions of binary azeotropes containing lower alcohols. The QSPR models for 64 binary azeotropes based on centroid approximation and weighted-contribution-factor approximation were established using the genetic function approximation(GFA) procedure in Materials Studio software, and a leave-one-out cross-validation procedure was *** tests of an additional 16 azeotropes were also investigated, and high determination coefficient values were obtained. The best QSPR models were explained in terms of the molecular structure of the azeotropes,and good predictive ability was obtained within acceptable prediction error levels.

关键词： QSPR Azeotropic temperature Azeotropic composition Genetic function approximation Binary azeotropes

CRISPR/Cas13a系统在大肠杆菌RNA编辑过程中的逃逸现象

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微生物学报 2024年第8期64卷 2998-3013页

作者：张悦许杰王珩瑜盛勇欧一新王斌张蓓康前进张丽青岛大学青岛医学院基础医学院山东青岛266071 上海交通大学生命科学技术学院微生物代谢国家重点实验室上海200240 上海交通大学代谢与发育国际联合合作实验室上海200240

【目的】在大肠杆菌(Escherichia coli)MG1655-ΔrecA和Escherichia coli DH10B中构建CRISPR/LshCas13a质粒干扰系统,通过靶向非必需基因lacZ和必需基因polA,分别分析RNA编辑实验中的逃逸现象。【方法】选取来自沙氏纤毛菌(Leptotrichia shahii)中的Cas13a蛋白编码基因LshCas13a,构建可诱导的CRISPR/LshCas13a的RNA编辑系统相关质粒,选取MG1655-ΔrecA和DH10B为研究对象。通过Crisporo算法,设计靶向lacZ和polA的CRISPR RNA(crRNA)序列,考察利用LshCas13a质粒干扰实验靶向lacZ和polA的逃逸现象。再通过对逃逸菌落的数量和序列分析评估LshCas13a系统的逃逸现象,结合PCR和Sanger测序技术探究LshCas13a系统的逃逸事件。选取通过插入序列(insertion sequence,IS)转座破坏LshCas13a系统的LshCas13a基因的逃逸菌落,通过监测OD_(600)进一步考察菌株的生长情况。【结果】利用LshCas13a系统靶向MG1655-ΔrecA和DH10B中的lacZ和polA,发现靶向lacZ时,MG1655-ΔrecA通过LshCas13a基因点突变和IS转座突变方式逃逸;靶向polA时,MG1655-ΔrecA和DH10B通过点突变LshCas13a基因、IS转座和突变crRNA的直接重复(direct repeat,DR)序列等方式逃逸。LshCas13a编码基因的突变促进了菌株生长的恢复。【结论】本研究利用LshCas13a质粒干扰系统研究了***宿主RNA编辑中的多样化逃逸现象,包括了染色体编码的IS介导的LshCas13a转座突变、LshCas13a点突变、crRNA的DR序列突变或重组等情况。本研究为进一步优化CRISPR/LshCas13a基因编辑系统奠定了基础。

关键词：大肠杆菌 CRISPR/LshCas13a 质粒干扰系统逃逸现象插入序列

Reinforcement learning based dynamic distributed routing scheme for mega LEO satellite networks

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Chinese Journal of Aeronautics 2023年第2期36卷 284-291页

作者： yixin HUANG Shufan WU Zeyu kang Zhongcheng MU Hai HUANG Xiaofeng WU Andrew Jack TANG Xuebin CHENG School of Aeronautics and Astronautics Shanghai Jiao Tong UniversityShanghai 200240China School of Astronautics Beihang UniversityBeijing 100191China School of Aerospace Mechanical and Mechatronic EngineeringThe University of SydneyCamperdown 2006Australia X-Laboratory The Second Academy of China Aerospace Science and Industry CorporationBeijing 100854China

Recently,mega Low Earth Orbit(LEO)Satellite Network(LSN)systems have gained more and more attention due to low latency,broadband communications and global coverage for ground *** of the primary challenges for LSN systems with inter-satellite links is the routing strategy calculation and maintenance,due to LSN constellation scale and dynamic network topology *** order to seek an efficient routing strategy,a Q-learning-based dynamic distributed Routing scheme for LSNs(QRLSN)is proposed in this *** achieve low end-toend delay and low network traffic overhead load in LSNs,QRLSN adopts a multi-objective optimization method to find the optimal next hop for forwarding data *** results demonstrate that the proposed scheme can effectively discover the initial routing strategy and provide long-term Quality of Service(QoS)optimization during the routing maintenance *** addition,comparison results demonstrate that QRLSN is superior to the virtual-topology-based shortest path routing algorithm.

关键词： LEO satellite networks Mega constellation Multi-objective optimization Routing algorithm Reinforcement learning

Facile Access to Polar-Functionalized Ultrahigh Molecular Weight Polyethylene at Ambient Conditions

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CCS Chemistry 2022年第5期4卷 1680-1694页

作者： Xiaoqiang Hu Xiaohui kang yixin Zhang Zhongbao Jian State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022 University of Science and Technology of China Hefei 230026 College of Pharmacy Dalian Medical UniversityDalian 116044

The most straightforward and potentially ideal route to produce polar-functionalized polyethylene is direct copolymerization of ethylene with polar ***,access to high-molecular-weight polar copolymers represents one of the biggest challenges in the field of olefin *** this contribution,we report a family of well-designed nickel catalysts that readily address this issue under convenient and highly desired ambient *** 1 bar at 30℃,polar-functionalized ultrahigh number-average molecular weight polyethylenes(UHMWPEs,Mn=0.83-1.10×10^(6) g mol^(−1))are directly *** highest average number of incorporated polar units per polymer chain is *** enhances copolymer molecular weights by two orders of magnitude relative to previous ***,this nickel catalyst family also exceptionally produces the highest number-average molecular weight polyethylenes(Mn=6.04×10^(6) g mol^(−1))at 1 *** Sterimol B1 steric parameter of nickel catalyst quantitatively correlates to polymer molecular *** insights from density functional theory(DFT)calculation reveal that the low barrier(10.4 kcal mol^(−1))of ethylene insertion as the rate-limiting step should be responsible for high activity and the formation of *** coordination-insertion approach is a striking contrast to the high energy free-radical approach.

关键词： coordination polymerization functionalized UHMWPE homogeneous catalysis nickel catalyst polyolefin

Time-course transcriptome landscape of achene development in lettuce

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Horticultural Plant Journal 2022年第1期8卷 99-109页

作者： Chen Luo Shenglin Wang kang Ning Zijing Chen Jingjing Yang yixin Wang Meixia Qi Qian Wang Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops Department of Vegetable ScienceCollege of HorticultureChina Agricultural UniversityBeijing 100193China

Lettuce(Lactuca sativa L.),which belongs to the large Asteraceae(Compositae)family,breeds by sexual reproduction and produces ***,lettuce seeds are achenes,which are defined as ***,few studies have described the morphological characteristics of the lettuce achenes,and genes essential for achene development are largely unknown in *** investigate the gene activity during achene development and determine the possible mechanisms that influence achene development in lettuce,we performed a time-course transcriptome analysis of lettuce achenes.A total of 27,390 expressed genes were detected at the five achene development *** investigated the gene expression patterns during achene development and identified the enriched biological processes at the corresponding *** Encyclopedia of Genes and Genomes and Gene Ontology analyses revealed a variety of transcriptomic similarities and differences at different achene development ***,transcription factors and phytohormones were found to play important roles during achene ***,we proposed a working model to illustrate the gene expression modules and possible molecular mechanisms underlying achene *** time-course transcriptome data also provide a foundation for future functional studies to reveal the genetic control of achene development in lettuce.

关键词： Lettuce Achene development Transcriptome Fruit Seed Transcription factor