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Surface Characteristics of Mussel-inspired Polydopamine Coating on Titanium Substrates

Journal of Wuhan University of Technology(Materials Science) 2014年第1期29卷 197-200页

作者：姜玲 JIN Guangchun kang jiyeon YU Lingmin YOON Wookang LIM MyungJin PAR K Ilsong LEE Minho JIN Dongchun Yantai Affiliated Hospital of Binzhou Medical University Oral Medical College Beihua University Department of Oral and Maxillofacial Surgery Hallym University Dongtan Sacred Heart Hospital Department of Dental Biomaterials and Institute of Oral Bioscience School of Dentistry BK21 Project Chonbuk National University Department of Veterinary Yanbian University

Mussel adhesive proteins are non-toxic, biodegradable and have low immunogenic qualities that make them highly attractive for medical purposes compared with man-made materials. 3,4-DOPA, which is a dopamine precursor, is a critical element for achieving mussel-like adhesive properties. In present study, polydopamine was coated onto the titanium surfaces by dipping in a 2 mg/mL dopamine solution in 10 mM Tris- HCl buffer at pH 8.5. EDX element analysis and Raman spectroscopic analysis of the surface were conducted to verify the formation of polydopamine. The surface characteristics of polydopamine-coated titanium plates were observed by SEM and AFM. Hydrophilicity and corrosion-resistance were also evaluated by static contact angle measurements and potentiodynamic polarization corrosion tests. The absorption intensity for pH-induced polymerization rapidly increased for the initial four hours and thereafter smoothly increased. EDX element analyses revealed that the content of carbon and oxygen increased and the content of titanium decreased after the coating process. In the Raman spectra, polydopamine resulted in two new peaks at approximately 1 370 and 1 570 cm^-1 resulting from the stretching and deformation of catechol. Microstructural features revealed that nanometer-sized bright granules were randomly distributed after coating, and these nanoparticles grew with increased coating time. The Ra values of polydopamine-coated titanium plates were showed to increase with coating time. Compared to the pure titanium curve, the inflection point of polydopamine-coated titanium was located at a higher corrosion potential and lower corrosion current density. Findings from our research suggested that polydopamine coatings offer a versatile approach for titanium surface modification.

关键词： surface modification titanium polydopamine surface characteristics

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A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system

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Journal of Integrative Plant Biology 2016年第8期58卷 705-712页

作者： Hyeran Kim Sang-Tae Kim Jahee Ryu Min Kyung Choi jiyeon Kweon Beum-Chang kang Hyo-Min Ahn Suji Bae Jungeun Kim Jin-Soo Kim Sang-Gyu Kim Center for Genome Engineering Institute for Basic Science Department of Chemistry Seoul National University

CRISPR-Cas9 system is now widely used to edit a target genome in animals and plants. Cas9 protein derived from Streptococcus pyogenes（Sp Cas9） cleaves double-stranded DNA targeted by a chimeric single-guide RNA（sg RNA）. For plant genome editing, Agrobacterium-mediated T-DNA transformation has been broadly used to express Cas9 proteins and sg RNAs under the control of Ca MV 35 S and U6/U3 promoter, respectively. We here developed a simple and high-throughput binary vector system to clone a 19 20 bp of sg RNA, which binds to the reverse complement of a target locus, in a large T-DNA binary vector containing an Sp Cas9 expressing cassette. Twostep cloning procedures：（1） annealing two target-specific oligonucleotides with overhangs specific to the Aar I restriction enzyme site of the binary vector; and（2） ligating the annealed oligonucleotides into the two Aar I sites of the vector, facilitate the high-throughput production of the positive clones. In addition, Cas9-coding sequence and U6/U3 promoter can be easily exchanged via the GatewayTMsystem and unique Eco RI/Xho I sites on the vector, respectively. We examined the mutation ratio and patterns when we transformed these constructs into Arabidopsis thaliana and a wild tobacco, Nicotiana attenuata. Our vector system will be useful to generate targeted large-scale knock-out lines of model as well as non-model plant.

关键词： Aar I-mediated sg RNA cloning CRISPR-Cas9 T-DNA binary vector Exchangeable U6/U3 promoter Gateway compatible Cas9 cloning

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Global public concern of childhood and adolescence suicide:a new perspective and new strategies for suicide prevention in the post-pandemic era

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World Journal of Pediatrics 2024年第9期20卷 872-900页

作者： Soeun Kim Jaeyu Park Hyeri Lee Hayeon Lee Selin Woo Rosie Kwon Sunyoung Kim Ai Koyanagi Lee Smith Masoud Rahmati Guillaume Fond Laurent Boyer Jiseung kang Jun Hyuk Lee jiyeon Oh Dong Keon Yon Center for Digital Health Medical Science Research InstituteKyung Hee University College of MedicineSeoulSouth Korea Department of Precision Medicine Kyung Hee University College of MedicineSeoulSouth Korea Department of Regulatory Science Kyung Hee UniversitySeoulSouth Korea Department of Family Medicine Kyung Hee University Medical CenterKyung Hee University College of MedicineSeoulSouth Korea Research and Development Unit Parc Sanitari Sant Joan de DeuBarcelonaSpain Centre for Health Performance and WellbeingAnglia Ruskin UniversityCambridgeUK Department of Physical Education and Sport Sciences Faculty of Literature and Human SciencesLorestan UniversityKhoramabadIran Department of Physical Education and Sport Sciences Faculty of Literature and HumanitiesVali-e-Asr University of RafsanjanRafsanjanIran CEReSS-Health Service Research and Quality of Life Center Assistance Publique-Hôpitaux de MarseilleAix Marseille UniversityMarseilleFrance Department of Anesthesia Critical Care and Pain MedicineMassachusetts General HospitalBostonMAUSA Division of Sleep Medicine Harvard Medical SchoolBostonMAUSA Health and Human Science University of Southern CaliforniaLos AngelesCAUSA Department of Medicine Kyung Hee University College of MedicineSeoulSouth Korea Department of Pediatrics Kyung Hee University Medical CenterKyung Hee University College of Medicine23 Kyungheedae-roDongdaemun-guSeoul02447South Korea

Background Suicide is the second leading cause of death in young people worldwide and is responsible for about 52,000 deaths annually in children and adolescents aged 5-19 ***,social,psychological,and behavioral factors play important roles in suicide *** traumatic events such as the COVID-19 pandemic may contribute to suicidal behaviors in young people,there is a need to understand the current status of suicide in adolescents,including its epidemiology,associated factors,the influence of the pandemic,and management *** sources We investigated global and regional suicide mortality rates among children and adolescents aged 5-19 years using data from the Global Burden of Diseases,Injuries,and Risk Factors Study(GBD)*** suicide mortality rates from 1990 to 2019 were examined in 204 countries and territories across six World Health Organization(WHO)***,we utilized electronic databases,including PubMed/MEDLINE and Scopus,and employed various combinations of terms such as“suicide”,“adolescents”,“youth”,“children”,“risk factors”,“COVID-19 pandemic”,“prevention”,and“intervention”to provide a narrative review on suicide within the pediatric population in the post-pandemic *** Despite the decreasing trend in the global suicide mortality rate from 1990 to 2019,it remains *** mortality rates from suicide by firearms or any other specified means were both greater in ***,Southeast Asia had the highest suicide rate among the six WHO *** COVID-19 pandemic seems to contribute to suicide risk in young people;thus,there is still a strong need to revisit appropriate management for suicidal children and adolescents during the *** The current narrative review integrates up-to-date knowledge on suicide epidemiology and the effects of the COVID-19 pandemic,risk factors,and intervention *** numerous studies have characterized trends in suicide among young people during the

关键词： COVID-19 Global Pandemic Suicide Children Adolescents

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