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食用菌学报 2020年第4期27卷 51-58页

作者：杨焕玲游华芳赵妍 kakumyan pattana 常婷婷余昌霞陈明杰上海市农业科学院食用菌研究所上海201403 安顺学院农学院贵州安顺561000 皇太后大学理学院泰国清莱57100

根据草菇(Volvariella volvacea)的锰超氧化物歧化酶(Mn-SOD)基因序列,构建重组质粒pBAR GPE1/Mn-SOD,转化大肠杆菌(Escherichia coli)Stbl3菌株,采用单因素实验对诱导剂IPTG浓度(0、0.2、0.5、1.0、1.5、2.0 mmol·L^-1)、诱导培养时间(0、2、4、6、8、10 h)及MnCl2浓度(0、0.2、0.5、1.0、1.5、2.0 mmol·L^-1)进行优化,以获得最优表达条件。在最优条件下分别进行耐热性、耐冷性及耐盐性分析,测定重组菌pBAR GPE1/Mn-SOD和对照菌pBAR GPE1的SOD酶活力及生长情况。结果表明:最优表达条件为IPTG浓度1 mmol·L^-1,诱导培养时间6 h,不需要添加MnCl2;在最优条件下,与对照菌相比,发现重组菌的SOD酶活力显著提高,生长情况较好,提高了转化菌株的耐热性、耐冷性和耐盐性。

关键词：草菇锰超氧化物歧化酶异源表达耐胁迫

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草菇废棉培养料的理化性质及木质纤维素变化分析

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分子植物育种 2021年第7期19卷 2364-2371页

作者：刘顺杰张亚茹陈明杰马丹丹 kakumyan pattana 郭倩赵妍上海海洋大学食品学院上海201306 上海市农业科学院食用菌研究所上海201403 School of Science Mae Fah Luang UniversityChiang Rai57100Thailand

为探究草菇生产中产量不稳定的内在原因,本研究通过测定废棉培养料不同时期的理化性状、木质纤维素含量及降解酶活性,对草菇(Volvariella volvacea)低产和高产批次基质的降解情况进行了对比分析。结果表明:在培养料的整个发酵阶段,两批... 详细信息

为探究草菇生产中产量不稳定的内在原因,本研究通过测定废棉培养料不同时期的理化性状、木质纤维素含量及降解酶活性,对草菇(Volvariella volvacea)低产和高产批次基质的降解情况进行了对比分析。结果表明:在培养料的整个发酵阶段,两批次的含水量和碳氮比均逐渐降低,灰分含量、含氮量则逐渐升高,同时高产批次的羧甲基纤维素酶(CMCase)和木聚糖酶活性一直显著高于低产批次。发酵结束时,高产批次的碳氮比较低产批次高4.60,p H值和含水量无明显差异。草菇生长发育阶段,高、低产两批次碳氮比的下降幅度分别为25.71%和12.77%,且前者纤维素相对含量的下降幅度显著大于后者。原基形成时低产批次的漆酶(Laccase)活性是高产批次的2.07倍,采收时低产批次子实体的平均单重较高产批次高6.66 g。本研究对草菇栽培基质的科学优化及工厂化生产进程的推进具有重要参考意义。

关键词：草菇废棉培养料木质纤维素酶活性

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A review of bambusicolous Ascomycota in China with an emphasis on species richness in southwest China

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Studies in Fungi 2022年第1期7卷 143-175页

作者： Hongbo Jiang Rungtiwa Phookamsak Sinang Hongsanan Darbhe J.Bhat Peter E.Mortimer Nakarin Suwannarach pattana kakumyan Jianchu Xu Centre for Mountain Futures Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Honghe Center for Mountain Futures Kunming Institute of BotanyChinese Academy of SciencesHonghe County 654400YunnanChina CAS Key Laboratory for Plant Biodiversity and Biogeography of East Asia(KLPB) Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanChina School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand World Agroforestry Centre East and Central Asia Office132 Lanhei RoadKunming 650201YunnanChina Research Center of Microbial Diversity and Sustainable Utilization Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Department of Entomology&Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Shenzhen Key Laboratory of Microbial Genetic Engineering College of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060GuangdongChina Formerly Department of BotanyGoa UniversityGoa 403206India No.128/1-J Azad Housing SocietyCurcaP.O.Goa Velha 403108India Yunnan Key Laboratory for Fungal Diversity and Green Development Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand

Bamboos not only provide socio-economic benefits to communities within the region,but also provide ecosystem services such as soil-water conservation,stabilization of sandy soils and restoration of soil *** ascomycetes refer to ascomycetous fungi living on any substrate of *** the largest group of fungi on bamboo,they play a significant ecological value in species composition and the structure of the fungal community,circulation of materials and energy flow of nutritional *** an effort to document the bambusicolous Ascomycota found in China,we assessed all major sources of academic literature,journal papers,and the USDA database(https://***/fungaldatabases/fungushost/***)for reports of these fungi from *** a result,we produced a systematic and comprehensive checklist of bambusicolous Ascomycota in *** names of fungi,bamboo host name,bamboo substrate,details of collected localities,references and latest classification for every bambusicolous ascomycete in China are also *** addition,we focused on the species richness of bambusicolous Ascomycota in China with an emphasis on southwest China.

关键词： southwest details Bamboo

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Mushroom cultivation for soil amendment and bioremediation

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Circular Agricultural Systems 2021年第1期1卷 73-86页

作者： Yuwei Hu Peter E.Mortimer Kevin D.Hyde pattana kakumyan Naritsada Thongklang Centre for Mountain Futures Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Center of Excellence in Fungal Research Mae Fah Luang University57100 Chiang RaiThailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Mushroom Research Foundation 128 M.3 Ban Pa Deng T.Pa PaeA.Mae TaengChiang Mai 50150Thailand

Intensive crop production,use of pesticides,and unsustainable farming practices are known to cause land degradation and soil *** have led to a decline in biodiversity and changes in the functional groups of soil *** physicochemical methods have been used to apply soil amendments to agricultural land,mushroom cultivation in agricultural land for soil improvement have been poorly ***-field mushroom cultivation is considered a good strategy for improving soil quality by reducing the input of chemical *** this paper,we list the edible mushroom species suitable for growing in fields and summarize the important role that mushroom field cultivation can play in soil erosion control,nutrient cycling,and the bioremediation of ***,symbiosis,assimilation,degradation,bioweathering,oxidation,biosorption,and bioconversion are all critical components of mushroom field *** has shown that field mushroom cultivation contributes to nutritional bioavailability while also promoting the degradation of pollutants and formation of soil *** soil amendment practices,a portion of agricultural waste can be converted into high-quality food and nutraceutical sources,and the remaining organic matter improves soil quality via fungal mycelial networks and the re-use of spent mushroom *** a small number of mushroom species have been used in the application of soil amendments in field *** review shows the need for further research into specific mushroom species for achieving different soil amendment goals in order to balance agricultural development with sustainable land management.

关键词： soil cultivation cycling

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The amazing potential of fungi:50 ways we can exploit fungi industrially

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Fungal Diversity 2019年第4期97卷 1-136页

作者： Kevin D.Hyde Jianchu Xu Sylvie Rapior Rajesh Jeewon Saisamorn Lumyong Allen Grace T.Niego Pranami D.Abeywickrama Janith V.S.Aluthmuhandiram Rashika S.Brahamanage Siraprapa Brooks Amornrat Chaiyasen K.W.Thilini Chethana Putarak Chomnunti Clara Chepkirui Boontiya Chuankid Nimali I.de Silva Mingkwan Doilom Craig Faulds Eleni Gentekaki Venkat Gopalan pattana kakumyan Dulanjalee Harishchandra Hridya Hemachandran Sinang Hongsanan Anuruddha Karunarathna Samantha C.Karunarathna Sehroon Khan Jaturong Kumla Ruvishika S.Jayawardena Jian-Kui Liu Ningguo Liu Thatsanee Luangharn Allan Patrick G.Macabeo Diana S.Marasinghe Dan Meeks Peter E.Mortimer Peter Mueller Sadia Nadir Karaba N.Nataraja Sureeporn Nontachaiyapoom Meghan O’Brien Watsana Penkhrue Chayanard Phukhamsakda Uma Shaanker Ramanan Achala R.Rathnayaka Resurreccion B.Sadaba Birthe Sandargo Binu C.Samarakoon Danushka S.Tennakoon Ramamoorthy Siva Wasan Sriprom T.S.Suryanarayanan Kanaporn Sujarit Nakarin Suwannarach Thitipone Suwunwong Benjarong Thongbai Naritsada Thongklang Deping Wei S.Nuwanthika Wijesinghe Jake Winiski Jiye Yan Erandi Yasanthika Marc Stadler Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand World Agroforestry Centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Mushroom Research Foundation 128 M.3 Ban Pa Deng T.PaPaeA.Mae TaengChiang Mai 50150Thailand Aix Marseille Université INRAUMR1163 BBF13288 Marseille Cedex 09France Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Center of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097People’s Republic of China Center of Chemical Innovation for Sustainability Mae Fah Luang UniversityTasudMuangChiang Rai 57100Thailand Center of Excellence in Microbial Diversity and Sustainable Utilization Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Center for Mountain Ecosystem Studies Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanPeople’s Republic of China Institute of Fungal Research School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengdu 611731People’s Republic of China Department Microbial Drugs Helmholtz Centre for Infection Researchand German Centre for Infection Research(DZIF)partner site Hannover-BraunschweigInhoffenstrasse 738124 BrunswickGermany Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Department of Chemistry and Biochemistry The Ohio State UniversityColumbusOH 43210USA Department of Chemistry University of Science and TechnologyBannuKhyber PakhtunkhwaPakistan Department of Crop Physiology University of Agricultural SciencesGKVKBengaluruKarnataka 560065India Department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai Univers

Fungi are an understudied,biotechnologically valuable group of *** to the immense range of habitats that fungi inhabit,and the consequent need to compete against a diverse array of other fungi,bacteria,and animals,fungi have developed numerous survival *** unique attributes of fungi thus herald great promise for their application in biotechnology and ***,fungi can be grown with relative ease,making production at scale *** search for fungal biodiversity,and the construction of a living fungi collection,both have incredible economic potential in locating organisms with novel industrial uses that will lead to novel *** manuscript reviews fifty ways in which fungi can potentially be utilized as *** provide notes and examples for each potential exploitation and give examples from our own work and the work of other notable *** also provide a flow chart that can be used to convince funding bodies of the importance of fungi for biotechnological research and as potential *** have provided the world with penicillin,lovastatin,and other globally significant medicines,and they remain an untapped resource with enormous industrial potential.

关键词： Biocontrol Biodiversity Biotechnology Food Fungi Mushrooms

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