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Insect Science 2007年第1期14卷 47-52页

作者： kai li gong-yin ye jia-ying zhu cui hu Institute of Insect Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou China

The last meal of sarcophagous maggots may be useful in identifying the species on whose flesh they have fed （the＂host＂species）. The DNA profile of the host species may indeed be detectable in the＂last meal＂. In this paper, mitochondrial DNA analysis of gut contents was used to identify the prior host of post-feeding larvae of Aldrichina grahami （Aldrich）（Diptera： Calliphoridae）. A modified logistic equation was fitted to estimate the probability of identifying the host under five different constant temperatures （16, 20, 24, 28 and 32 ℃）. Our results shows that the detected time ranged from a maximum of 24 h at 32℃ to 42 h at 16℃ and a minimum of 12 h at 32~C to 30 h at 16℃. Furthermore, the host detection time was also calculated to give the maximal time after larval hatching from the egg. These results indicate that, in criminal cases where the maggots stray from the corpse, the last meal of the larvae should not be overlooked as potentially critical evidence.

关键词： Cytochrome b gene detectable time forensic entomology gut post-feeding

Focal surface attitude detection for LAMOST

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Research in Astronomy and Astrophysics 2020年第11期20卷 263-272页

作者： Tian-zhu hu Yong Zhang Xiang-Qun cui ye-Ping li Xiu-Shan Pan ying Fu National Astronomical Observatories/Nanjing Institute of Astronomical Optics&Technology Chinese Academy of ScienceNanjing 210042China CAS Key Laboratory of Astronomical Optics&Technology Nanjing Institute of Astronomical Optics&TechnologyNanjing 210042China University of Chinese Academy of Sciences Beijing 100049China

With telescope apertures becoming larger and larger,the deployment of large-field telescopes is becoming increasingly ***,optical path calibration is necessary to ensure the image quality of large-field and large-diameter *** particular,focal plane attitude calibration is an essential optical path calibration technique that has a direct impact on image *** this paper,a focal plane attitude detection method using eight acquisition cameras is proposed based on the calibration requirements of the wide-field telescope,LAMOST(Large Sky Area Multi-Object Fiber Spectroscopic Telescope).Comparison of simulation and experimental results shows that the detection accuracy of the proposed method can reach 30 *** additional testing and verification,this method could be used to facilitate regular focal plane attitude calibration for LAMOST as well as other large-field telescopes.

关键词： methods data analysis techniques high angular resolution techniques image processing

High pressure phase boundaries of AgNbO_(3)

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Journal of Materiomics 2024年第2期10卷 431-439页

作者： kai Dai Anyang cui Yafang li Zhen liu Yuting Yan kai jiang Jinzhong Zhang liyan Shang liangqing zhu Yawei li Genshui Wang Zhigao hu Technical Center for Multifunctional Magneto-Optical Spectroscopy(Shanghai) Engineering Research Center of Nanophotonics&Advanced Instrument(Ministry of Education)Department of PhysicsSchool of Physics and Electronic ScienceEast China Normal UniversityShanghai200241China Key Laboratory of Optoelectronic Material and Device Department of PhysicsShanghai Normal UniversityShanghai200234China Chongqing Key Laboratory of Precision Optics Chongqing Institute of East China Normal UniversityChongqing401120China Key Laboratory of Inorganic Functional Materials and Devices Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuanShanxi030006China

The external pressure is one of the essential parameters for regulating the structure and energy con-version properties of antiferroelectric AgNbO_(3).For pure AgNbO_(3),however,there has been still a blank of its real lattice structure under the stress ***,high-pressure lattice structures and phase transitions of AgNbO_(3)have been explored by spectroscopic experiments and theoretical models.A successive phase transition process from Pbcm to C222_(1) to P2_(1) has been observed at the pressure range of 0-30 GPa,associated with displacive-type characterized by soft-mode *** that the paraelectric phase cannot be achieved under high-pressure at room ***,the competition of long-range Coulomb force,short-range interatomic interaction,and covalent interaction in AgNbO_(3)lattice were demonstrated under the stress *** present work can provide fundamental guidelines to reveal the high-pressure phase transitions of AgNbO_(3),which will open up possibilities for the designing device with functional properties at extremes.

关键词： temperature field phase

Defocus spot detection of astronomical optical system

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Research in Astronomy and Astrophysics 2020年第3期20卷 162-169页

作者： Tian-zhu hu Yong Zhang Xiang-Qun cui ye-Ping li hua Bai Xiu-Shan Pan National Astronomical Observatories/Nanjing Institute of Astronomical Optics&Tech no logy.Chinese Academy of Science Nanjing 210042China Key Laboratory of Astronomical Optics&Technology Nanjing Institute of Astronomical Optics&TechnologyChinese Academy of SciencesNanjing 210042China University of the Chinese Academy of Sciences Beijing 100049China

Defocusing spot size detection is especially essential for aberration analysis and correction of optical systems. In the case of far defocusing, the celestial forms a pupil image on the detector, and the size of the image is linearly changed with the defocusing distance, and can be used to correct the optical system and analyze the image quality. Based on the focal plane attitude detection of Large Sky Area MultiObject Fiber Spectroscopy Telescope(LAMOST), this paper uses a variety of methods to detect the size of the defocusing spot of LAMOST telescope. For the particularity of the spot, the average value spacing algorithm, the peak value spacing algorithm, the ellipse fitting algorithm, and the multi-peak Gaussian fitting algorithm are used to detect the spot size. This paper will introduce these four methods, in which the average value spacing algorithm is proposed by the author of this paper. The advantages and disadvantages of the four methods are compared. The experimental results show that the average value spacing algorithm can achieve better accuracy of spot size detection in the four algorithms.

关键词： methods data analysis-techniques image processing-telescopes

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蝶蛹金小蜂毒液蛋白不同提取分离方法的比较

昆虫学报 2008年第11期51卷 1144-1150页

作者：吴玛莉朱家颖胡萃叶恭银浙江大学昆虫科学研究所杭州310029

为了建立蝶蛹金小蜂Pteromalus puparum毒液抑制寄主血细胞免疫活性组分合适的分离纯化方法,就等电点沉淀法、乙醇沉淀法、75%硫酸铵沉淀法、75%硫酸铵沉淀法+40℃加热处理法,以及75%硫酸铵沉淀法分别与3种不同滤膜的分子大小截留法的... 详细信息

为了建立蝶蛹金小蜂Pteromalus puparum毒液抑制寄主血细胞免疫活性组分合适的分离纯化方法,就等电点沉淀法、乙醇沉淀法、75%硫酸铵沉淀法、75%硫酸铵沉淀法+40℃加热处理法,以及75%硫酸铵沉淀法分别与3种不同滤膜的分子大小截留法的组合等7种方法对毒液蛋白分离效果及活性的影响进行了比较。结果表明:等电点沉淀法获得的组分抑制寄主菜粉蝶Pieris rapae离体血细胞延展和包囊的活性最强,乙醇沉淀法次之,75%硫酸铵沉淀法最弱。从蛋白组分的SDS-PAGE图谱来看,等电点沉淀法获得毒液组分相对最纯,仅有3条主要谱带,分子量大小在45～116.2kDa范围内;乙醇沉淀法次之,有5条主要谱带,分子量大小在24～116.2kDa范围内;硫酸铵沉淀法的谱带组成与毒液蛋白粗提液相似。3种分子大小截留法获得的毒液组分的活性分析表明,强活性组分分子量大小可能都大于100kDa。综合认为,7种方法中以等电点沉淀法提取分离蝶蛹金小蜂毒液蛋白相对为最适。

关键词：蝶蛹金小蜂莱粉蝶毒液分离血细胞延展血细胞包囊

双向凝胶电泳图谱用于常见尸食性蝇类初孵幼虫的鉴别

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昆虫学报 2005年第4期48卷 576-581页

作者：李凯叶恭银胡萃浙江大学应用昆虫学研究所杭州310029

双向凝胶电泳分析技术已在生物科学各领域被广泛应用,蛋白质组作图的意义已经日益显现。通过对4种常见尸食性蝇类初孵幼虫蛋白质组双向凝胶电泳和图象分析,发现各种类间双向凝胶电泳图谱差异显著,并对相应的等电点和相对分子量进行聚类... 详细信息

双向凝胶电泳分析技术已在生物科学各领域被广泛应用,蛋白质组作图的意义已经日益显现。通过对4种常见尸食性蝇类初孵幼虫蛋白质组双向凝胶电泳和图象分析,发现各种类间双向凝胶电泳图谱差异显著,并对相应的等电点和相对分子量进行聚类和判别分析,结果表明,建立合适的尸食性蝇类初孵幼虫的双向凝胶电泳图谱可用于鉴别形态学极易混淆的昆虫种类。

关键词：蛋白质组双向凝胶电泳法医昆虫学尸食性蝇类种类鉴别

Winter cover crops alter methanotrophs community structure in a double-rice paddy soil

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Journal of Integrative Agriculture 2016年第3期15卷 553-565页

作者： liU Jing-na zhu Bo YI li-xia DAI Hong-cui XU He-shui ZHANG kai hu Yue-gao ZENG Zhao-hai College of Agriculture and Biotechnology China Agricultural University School of Agronomy Yangtze University

Methanotrophs play a vital role in the mitigation of methane emission from soils. However, the influences of cover crops incorporation on paddy soil methanotrophic community structure have not been fully understood. In this study, the impacts of two winter cover crops（Chinese milk vetch（Astragalus sinicus L.） and ryegrass（Lolium multiflorum Lam.）, representing leguminous and non-leguminous cover crops, respectively） on community structure and abundance of methanotrophs were evaluated by using PCR-DGGE（polymerase chain reaction-denaturing gradient gel electrophoresis） and real-time PCR technology in a double-rice cropping system from South China. Four treatments were established in a completely randomized block design： 1） double-rice cropping without nitrogen fertilizer application, CK; 2） double-rice cropping with chemical nitrogen fertilizer application（200 kg ha^（–1） urea for entire double-rice season）, CF; 3） Chinese milk vetch cropping followed by double-rice cultivation with Chinese milk vetch incorporation, MV; 4） ryegrass cropping followed by double-rice cultivation with ryegrass incorporation, RG. Results showed that cultivating Chinese milk vetch and ryegrass in fallow season decreased soil bulk density and increased rice yield in different extents by comparison with CK. Additionally, methanotrophic bacterial abundance and community structure changed significantly with rice growth. Methanotrophic bacterial pmo A gene copies in four treatments were higher during late-rice season（3.18×10^7 to 10.28×10^7 copies g^–1 dry soil） by comparison with early-rice season（2.1×10^7 to 9.62×10^7 copies g^–1 dry soil）. Type Ⅰ methanotrophs absolutely predominated during early-rice season. However, the advantage of type Ⅰ methanotrophs kept narrowing during entire double-rice season and both types Ⅰ and Ⅱ methanotrophs dominated at later stage of late-rice.

关键词： Chinese milk vetch ryegrass methanotrophic bacteria community structure double-rice

Rose without prickle: genomic insights linked to moisture adaptation

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National Science Review 2021年第12期8卷 74-85页

作者： Mi-Cai Zhong Xiao-Dong jiang Guo-Qian Yang Wei-hua cui Zhi-Quan Suo Wei-jia Wang Yi-Bo Sun Dan Wang Xin-Chao Cheng Xu-Ming li Xue Dong kai-Xue Tang De-zhu li Jin-Yong hu CAS Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of Sciences University of Chinese Academy of Sciences Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of Sciences Flower Research Institute Yunnan Agricultural Academy of Sciences Biomarker Technologies Corporation

Prickles act against herbivores, pathogens or mechanical injury, while also preventing water loss. However,whether prickles have new function and the molecular genetics of prickle patterning remain poorly explored. Here, we generated a high-quality reference genome assembly for ‘Basye’s Thornless’(BT), a prickle-free cultivar of Rosa wichuraiana, to identify genetic elements related to stem prickle *** BT genome harbors a high level of sequence diversity in itself and with cultivar ‘Old Blush’(R. chinensis), a founder genotype in rose domestication. Inheritance of stem prickle density was determined and two QTL were identified. Differentially expressed genes in QTL were involved in water-related functions, suggesting that prickle density may hitchhike with adaptations to moist environments. While the prickle-related gene-regulatory-network(GRN) was highly conserved, the expression variation of key candidate genes was associated with prickle density. Our study provides fundamental resources and insights for genome evolution in the Rosaceae. Ongoing efforts on identification of the molecular bases for key rose traits may lead to improvements for horticultural markets.

关键词： Rosa wichuraiana ‘Basye's Thornless’ prickle-free water storage gene-regulatory-network (GRN) QTL

Failure mechanisms and destruction characteristics of cemented coal gangue backfill under compression effect of non-uniform load

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Journal of Central South University 2024年第8期31卷 2676-2693页

作者： FENG Guo-rui GUO Wei QI Ting-ye li zhu cui jia-qing WANG Hao-chen cui ye-kai MA Jing-kai College of Mining Engineering Taiyuan University of TechnologyTaiyuan 030024China Shanxi Province Research Center of Green Mining Engineering Technology Taiyuan 030024China Shanxi Province Coal-based Resources Green and High-efficiency Development Engineering Center Taiyuan 030024China Shanxi Key Laboratory of Mine Rock Strata Control and Disaster Prevention Taiyuan 030024China Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering Taiyuan 030024China

Backfill mining is one of the most important technical means for controlling strata movement and reducing surface subsidence and environmental damage during exploitation of underground coal resources. Ensuring the stability of the backfill bodies is the primary prerequisite for maintaining the safety of the backfilling working face, and the loading characteristics of backfill are closely related to the deformation and subsidence of the roof. Elastic thin plate model was used to explore the non-uniform subsidence law of the roof, and then the non-uniform distribution characteristics of backfill bodies’ load were revealed. Through a self-developed non-uniform loading device combined with acoustic emission (AE) and digital image correlation (DIC) monitoring technology, the synergistic dynamic evolution law of the bearing capacity, apparent crack, and internal fracture of cemented coal gangue backfills (CCGBs) under loads with different degrees of non-uniformity was deeply explored. The results showed that: 1) The uniaxial compressive strength (UCS) of CCGB increased and then decreased with an increase in the degree of non-uniformity of load (DNL). About 40% of DNL was the inflection point of DNL-UCS curve and when DNL exceeded 40%, the strength decreased in a cliff-like manner;2) A positive correlation was observed between the AE ringing count and UCS during the loading process of the specimen, which was manifested by a higher AE ringing count of the high-strength specimen. 3) Shear cracks gradually increased and failure mode of specimens gradually changed from “X” type dominated by tension cracks to inverted “Y” type dominated by shear cracks with an increase in DNL, and the crack opening displacement at the peak stress decreased and then increased. The crack opening displacement at 40% of the DNL was the smallest. This was consistent with the judgment of crack size based on the AE b-value, i. e., it showed the typical characteristics of “small b-value-large crack and

关键词： cemented coal gangue backfill non-uniform load degree of non-uniformity of load failure mode crack opening displacement

Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity

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Signal Transduction and Targeted Therapy 2024年第1期9卷 259-270页

作者： Yan Sun Xiu-Na Yang Shuang-Shuang Yang Yi-zhu Lyu Bing Zhang kai-Wen liu Na li jia-Chen cui Guang-Xiang huang Cheng-lin liu Jie Xu jian-Qing Mi zhu Chen Xiao-hu Fan Sai-Juan Chen Shuo Chen Shanghai Institute of Hematology National Research Center for Translational MedicineState Key Laboratory of Medical GenomicsRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Institute for Advanced Immunochemical Studies and School of life Science and Technology ShanghaiTech UniversityShanghai 201210China Department of Hematology Second Hospital of Dalian Medical UniversityDalian 116023China National Facility for Protein Science Shanghai Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China Legend Biotech China Nanjing 211112China Shanghai Immune Therapy Institute Shanghai Cancer InstituteState Key Laboratory of Oncogenes and Related GenesRenji Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200127China Wondercel Biotechnology Shenzhen 518052China

ligand-induced receptor dimerization or oligomerization is a widespread mechanism for ensuring communication specificity,safeguarding receptor activation,and facilitating amplification of signal transduction across the cellular ***,cell-surface antigeninduced multimerization(dubbed AIM herein)has not yet been consciously leveraged in chimeric antigen receptor(CAR)engineering for enriching T cell-based *** co-developed ciltacabtagene autoleucel(cilta-cel),whose CAR incorporates two B-cell maturation antigen(BCMA)-targeted nanobodies in tandem,for treating multiple *** we elucidated a structural and functional model in which BCMA-induced cilta-cel CAR multimerization amplifies myeloma-targeted T cell-mediated *** analysis of BCMA–nanobody complexes revealed atomic details of antigen–antibody hetero-multimerization whilst analytical ultracentrifugation and small-angle X-ray scattering characterized interdependent BCMA apposition and CAR juxtaposition in ***-induced nanobody CAR multimerization enhanced cytotoxicity,alongside elevated immune synapse formation and cytotoxicity-mediating cytokine release,towards myeloma-derived *** results provide a framework for contemplating the AIM approach in designing next-generation CARs.

关键词： cytotoxicity Antigen elevated