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Efficient expression of codon-adapted human acetaldehyde dehydrogenase 2 cDnA with 6×His tag in Pichia pastoris

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Science China(Life Sciences) 2009年第10期52卷 935-941页

作者： ZHAO YuFeng1,2, LEI Mingke2, WU YuanXin2, ZHAnG ZiSheng3 & WAnG CunWen2 1 State key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agriculture University, Wuhan 430070, China 2 key Laboratory for Green Chemical Processes of the Ministry of Education, Hubei key Lab of novel Reactor & Green Chemical Technology, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430074, China 3 Department of Chemical Engineering, University of Ottawa, Ottawa, Canada k1n 6n5 State key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agriculture University Wuhan China key Laboratory for Green Chemical Processes of the Ministry of Education Hubei Key Lab of Novel Reactor & Green Chemical Technology School of Chemical Engineering and Pharmacy Wuhan Institute of Technology Wuhan China Department of Chemical Engineering University of Ottawa Ottawa Canada

Human mitochondrial acetaldehyde dehydrogenase 2 (ALDH2) catalyzes the oxidation of acetaldehyde to acetic acid. Therefore, ALDH2 has therapeutic potential in detoxification of acetaldehyde. Further-more, ALDH2 catalyzes nitroglycerin to nitrate and 1, 2-glyceryldinitrate during therapy for angina pectoris, myocardial infarction, and heart failure. Large quantities of ALDH2 will be needed for potential clinical practice. In this study, Pichia pastoris was used as a platform for expression of human ALDH2. Based on the ALDH2*1 cDnA sequence, we designed ALDH2 cDnA by choosing the P. pastoris preferred codons and by decreasing the G + C content level. The sequence was synthesized using the overlap extension PCR method. The cDnA and 6×His tags were subcloned into the plasmid pPIC9k. The recombinant protein was expressed in P. pastoris GS115 and purified using ni2+-Sepharose affinity chromatography. The amount of secreted protein in the culture was 80 mg/L in shake-flask cultivation and 260 mg/L in high-density bioreactor fermentation. Secreted ALDH2 was easily purified from the culture supernatant by using ni2+-Sepharose affinity chromatography. After purification of the fermentation supernatant, the enzyme had a specific activity of 1.2 U/mg protein. The yield was about 16 mg/L in a shake flask culture of P. pastoris GS115 which contained the original human ALDH2*1 cDnA.

关键词： acetaldehyde dehydrogenase codon optimization overlap extension PCR Pichia pastoris.

Monophyly of the ring-forming group in Diplopoda(Myriapoda,Arthropoda) based on SSU and LSU ribosomal RnA sequences

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Progress in natural Science:Materials International 2009年第10期19卷 1297-1303页

作者： Peiyun Conga,b,Xuhua Xiac,Qun Yanga,a State key Laboratory of Palaeobiology and Strategraphy,nanjing Institute of Geology and Palaeontology,Chinese Academy of Sciences,nanjing 210008,China b Yunnan key Laboratory for Paleobiology,Yunnan University,kunming 650091,China c Department of Biology,University of Ottawa,Ont.,Canada k1n 6n5 State key Laboratory of Palaeobiology and Strategraphy Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences Nanjing 210008 China Yunnan key Laboratory for Paleobiology Yunnan University Kunming 650091 China Department of Biology University of Ottawa Ont. Canada K1N 6N5

Two controversies exist in the phylogeny of the derived millipedes(Diplopoda).The first is whether millipedes with a fusion ring,including Polydesmida,Spirobolida,Spirostreptida and Julida,form a monophyletic group(the ring-forming group).The second concerns the phylogenetic relationship within the three orders of Juliformia,***,Spirostreptida and *** resolve these phylogenetic controversies,we sequenced 18S and 28S rDnA from six millipede orders and retrieved several homologous sequences from *** results give robust support to the monophyly of the ring-forming group based on maximum parsimony methods,max-imum likelihood methods and Bayesian *** monophyly of the ring-forming group suggests that the fusion of segment sclerites might have occurred only once during millipede evolutionary *** also established a sister-group relationship between Spirobol-ida and Spirostreptida within Juliformia after eliminating a short-branch attraction phenomenon,which is consistent with that from the mitochondrial genome analysis.

关键词： Diplopoda Ring-forming group Ribosomal RnA Monophyly Short-branch attraction

The Optimization Issues In An Agile All-Photonic Backbone network

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The Optimization Issues In An Agile All-Photonic Backbone Ne...

Compression properties of cost-efficient porous expanded clay reinforced AA7075 syntactic foams fabricated by industrial-oriented die casting technology

Asia-pacific Optical Communications 2004

作者： Yiming Zhang Oliver W. W. Yang and Yihua Zhai CCnR Lab School of Information Technology and Engineering Univ. of Ottawa Ottawa Ontario Canada k1n 6n5

The Agile All-photonic Backbone network (AAPn) architecture has been proposed by the telecommunication industry as a potential candidate for the ultra high speed next Generation Optical network (nGOn)

关键词： Agile Optical networks AAPn network Optimization WDM networks

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China Foundry 2024年第1期21卷 60-70页

作者： İsmail Cem Akgün Çağın Bolat Ali Gökşenli Faculty of Mechanical Engineering Istanbul Technical University34437İstanbulTurkey Faculty of Engineering Samsun University55420SamsunTurkey

In today’s manufacturing industries,hard competition between rival firms makes it compulsory for researchers to design lighter and cheaper machine components due to the megatrends of cost-effectiveness and *** this point,aluminum syntactic foams(ASFs)are new-generation engineering composites and come into the upfront as a *** to their features like low density,sufficient elongation,and perfect energy absorption ability,these advanced foams have been considerably seductive for many industrial sectors *** this study,an industrial-oriented automatic die casting technology was used for the first time to manufacture the combination of AA7075/porous expanded clay(PEC).Micro evaluations(optical and FESEM)reveal that there is a homogenous particle distribution in the foam samples,and inspections are compatible with the other ASF ***,T6 aging heat treatment was operated on one half of the produced foams to explore the probable impact of aging on the compressive *** results show that PEC particles can be an alternative to expensive hollow spheres used in the previous ***,a favorable relationship is ascertained between the aging treatment and mechanical properties such as compression strength and plateau strength.

关键词： die casting porous materials metal matrix sytanctic foams expanded clay compressive deformation

Dispersion effect of nano-structure pyrolytic carbon on mechanical,electrical,and microstructural characteristics of cement mortar composite

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International Journal of Smart and nano Materials 2024年第3期15卷 534-578页

作者： n.k.karthikeyan S.Elavenil School of Civil Engineering Vellore Institute of TechnologyChennaiIndia

In material science,converting waste into nanomaterials by green technologies highlights the interest in producing sus-tainable carbon nanomaterial(SCn).This study first-ever uti-lized tyre-char as a nano-Structure Pyrolytic Carbon(nSPC)to develop electrically conductive *** other car-bon nanomaterials,the efficiency of nSPC particle as an SCn in developing electrically conductive composites was investi-gated in terms of dispersion,mechanical,water absorption,electrical resistivity,and *** results of sedi-mentation and zeta potential(-19 mV)showed the excellent dispersion of nSPC particle in water with the combined effect of superplasticizer and *** compressive(40.37%)and flexural strength(10.76%)than that of plain cement mortar composite(CMC)is achieved with 2 wt.%and 1.5 wt.%of nSPC particle,*** water absorption rate and volume of permeable voids were reduced,exhibiting less *** percolation zone of AC and DC resis-tivity of nSPC composite ranges between 1 and 2 wt.%,and the established percolation threshold is at 2 wt.%.XRD analysis showed C-S-H formation consistently increased with curing age,and HR-SEM with EDAX analysis exhibits the dense micro-structure and well-distributed nSPC particle at the *** experimental outcomes significantly enhanced the relia-bility and provided a framework for tailoring nSPC particle as SCn for developing electrically conductive composite.

关键词： Sustainable nanomaterial dispersion mechanical properties electrical resistivity microstructure analysis

Evaluation of slope stability through rock mass classification and kinematic analysis of some major slopes along nH-1A from Ramban to Banihal, north Western Himalayas

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第1期16卷 167-182页

作者： Amit Jaiswal A.k.Verma T.n.Singh Department of Civil and Environmental Engineering Indian Institute of Technology PatnaPatna801106India

The network of Himalayan roadways and highways connects some remote regions of valleys or hill slopes,which is vital for India’s socio-economic *** to natural and artificial factors,frequency of slope instabilities along the networks has been increasing over last few *** of stability of natural and artificial slopes due to construction of these connecting road networks is significant in safely executing these roads throughout the *** rock mass classification methods are generally used to assess the strength and deformability of rock *** study assesses slope stability along the nH-1A of Ramban district of north Western *** structurally and non-structurally controlled rock mass classification systems have been applied to assess the stability conditions of 14 *** evaluating the stability of these slopes,kinematic analysis was performed along with geological strength index(GSI),rock mass rating(RMR),continuous slope mass rating(CoSMR),slope mass rating(SMR),and Q-slope in the present *** SMR gives three slopes as completely unstable while CoSMR suggests four slopes as completely *** stability of all slopes was also analyzed using a design chart under dynamic and static conditions by slope stability rating(SSR)for the factor of safety(FoS)of 1.2 and 1 respectively.Q-slope with probability of failure(PoF)1%gives two slopes as stable *** slope angle has been determined based on the Q-slope safe angle equation and SSR design chart based on the *** value ranges given by different empirical classifications were RMR(37-74),GSI(27.3-58.5),SMR(11-59),and CoSMR(3.39-74.56).Good relationship was found among RMR&SSR and RMR&GSI with correlation coefficient(R 2)value of 0.815 and 0.6866,***,a comparative stability of all these slopes based on the above classification has been performed to identify the most critical slope along this road.

关键词： Rock mass classification kinematic analysis Slope stability Himalayan road Static and dynamic conditions

RBC aggregation,deformation and adhesion to endothelium:Role of nitric oxide derived from L-Arginine and sodium nitroprusside

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Journal of Innovative Optical Health Sciences 2024年第5期17卷 53-65页

作者： M.k.Maksimov P.B.Ermolinskiy O.n.Scheglovitova n.n.Sklyankina A.V.Muravyov A.E.Lugovtsov A.V.Priezzhev Faculty of Physics M.V.Lomonosov Moscow State University bld.1subbuilding.2Leninskie goryMoscow 11991Russia n.F.Gamaleya national Research Center for Epidemiology and Microbiology 18 Gamaleya StreetMoscow 123098Russia k.D.Ushinsky Yaroslavl State Pedagogical University 108/1 Respublikanskaya StreetYaroslavl 150000Russia

Red blood cells(RBCs)are the most abundant human blood *** aggregation and deformation strongly determine blood viscosity which impacts hemorheology and *** turn,RBC properties depend on di®erent endogenous and exogenous *** such factor is nitric oxide(nO),which is mainly produced by endothelial cells(EC)from L-arginine amino acid in the circulatory *** the mechanisms of the RBC-endothelium interplay are not clear up to date and considering its possible clinical importance,the aims of this study are to investigate in vitro:(1)The e®ect of L-arginine induced nO on RBC aggregation and adhesion to endothelium;(2)the nO e®ect on RBC aggregation and deformation induced by L-arginine and sodium nitroprusside without the presence of endothelium in the *** RBC aggregation and adhesion to a monolayer of EC were studied using optical tweezers(OT).The RBC deformability and aggregation without endothelium in the samples were studied using the°ow chamber method and Myrenne *** con¯rmed that nO increases deformability and decreases aggregation of *** showed that the soluble guanylate cyclase pathway appears to be the only nO signaling pathway *** the samples with the endothelium,the\bell-shaped"dependence of RBC aggregation force on L-arginine concentration was observed,which improves our knowledge about the process of nO production by ***,data related to L-arginine accumulation by endothelium were obtained:necessity of the presence of extracellular L-arginine stated by other authors was put under *** our study,nO decreased the RBC-endothelium adhesion,however,the tendency appeared to be weak and was not con¯rmed in another set of *** our knowledge,this is the¯rst attempt to measure the forces of RBC adhesion to endothelium monolayer with OT.

关键词： Red blood cells aggregation L-arginine endothelium optical tweezers Myrenne flow chamber

Chebyshev polynomial-based Ritz method for thermal buckling and free vibration behaviors of metal foam beams

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Applied Mathematics and Mechanics(English Edition) 2024年第5期45卷 891-910页

作者： n.D.nGUYEn T.n.nGUYEn Faculty of Civil Engineering Ho Chi Minh City University of Technology and EducationHo Chi Minh City 71309Viet Nam Faculty of Engineering and Technology Kien Giang UniversityChau Thanh 92000Viet Nam

This study presents the Chebyshev polynomials-based Ritz method to examine the thermal buckling and free vibration characteristics of metal foam *** analyses include three models for porosity distribution and two scenarios for thermal *** material properties are assessed under two conditions,i.e.,temperature dependence and temperature *** theoretical framework for the beams is based on the higher-order shear deformation theory,which incorporates shear deformations with higher-order *** governing equations are established from the Lagrange equations,and the beam displacement fields are approximated by the Chebyshev *** simulations are performed to evaluate the effects of thermal load,slenderness,boundary condition(BC),and porosity distribution on the buckling and vibration behaviors of metal foam *** findings highlight the significant influence of temperature-dependent(TD)material properties on metal foam beams'buckling and vibration responses.

关键词： Ritz method Chebyshev function buckling vibration metal foam beam higher-order beam theory(HOBT)

Stability Analysis of DC Microgrid Clusters Based on Step-by-step System Matrix Building Algorithm

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Journal of Modern Power Systems and Clean Energy 2024年第3期12卷 900-912页

作者： k.Jithin n.Mayadevi R.Hari kumar V.P.Mini the Department of Electrical Engineering College of Engineering TrivandrumAPJ Abdul Kalam Technological University(APJAKTU)ThiruvananthapuramIndia

DC microgrids(DCMGs)are made up of a network of sources and loads that are connected by a number of power electronic converters(PECs).The increase in the number of these PECs instigates major concerns in system *** interconnecting the microgrids to form a cluster,the system stability must be *** paper proposes a novel stepby-step system matrix building(SMB)algorithm to update the system matrix of an existing DCMG cluster when a new microgrid is added to the cluster through a distribution *** stability of the individual DCMGs and the DCMG cluster is analyzed using the eigenvalue ***,the particle swarm optimization(PSO)algorithm is used to retune the controller gains if the newly formed cluster is not *** simulation of the DCMG cluster is carried out in MATLAB/Simulink environment to test the proposed *** results are also validated using the OP4510 real-time simulator(RTS).

关键词： Converter DC microgrid cluster eigenvalue interconnection particle swarm optimization system stability system matrix