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Application of droplet digital PCR in detection of seed-transmitted pathogen Acidovorax citrulli

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Journal of Integrative Agriculture 2020年第2期19卷 561-569页

作者： LU Yu ZHANG Hai-jun ZHAO Zi-jing WEN Chang-long WU Ping SONG Shun-hua YU Shuan-cang Luo Lai-xin XU Xiu-lan Beijing key Laboratory of Vegetable Germplasms Improvement Beijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China Supervision Inspection and Test Center of Vegetable Seed Quality of Ministry of Agriculture and Rural AffairsBeijing 100097P.R.China Beijing key Laboratory of Seed Disease Testing and Control College of Plant PathologyChina Agricultural UniversityBeijing 100093P.R.China

Bacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry *** pathogen is seedtransmitted,so seed detection to prevent distribution of contaminated seed is crucial in disease *** this study,we adapted a quantitative real-time PCR(qPCR)assay to droplet digital PCR(ddPCR)format for *** detection by optimizing reaction *** performance of ddPCR in detecting *** pure culture,DNA,infested watermelon/melon seed and commercial seed samples were compared with multiplex PCR,qPCR,and dilution plating *** lowest concentrations detected(LCD)by ddPCR reached up to 2 fg DNA,and 102 CFU mL–1 bacterial cells,which were ten times more sensitive than those of the *** testing artificially infested watermelon and melon seed,0.1%infestation level was detectable using ddPCR and dilution plating *** 26 positive samples were identified in 201 commercial seed samples through ddPCR,which was the highest positive number among all the *** detection sensitivity achieved by ddPCR demonstrated a promising technique for improving seed-transmitted pathogen detection threshold in the future.

关键词： bacterial fruit blotch Acidovorax citrulli droplet digital PCR seed detection quantitative real-time PCR

Myocardial infarction following recombinant tissue plasminogen activator treatment for acute ischemic stroke： a dangerous complication

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Chinese Medical Journal 2012年第15期125卷 2775-2776页

作者： ZHOU Zhi-gang WANG Rui-lan YU kang-long Department of Emergency and Intensive Care Medicine ShanghaiFirst People's Hospital Shanghai Jiao Tong University Shanghai201620 China

Thrombolysis with intravenous tissue plasminogen activator （t-PA） is currently an approved therapy for patients with acute ischemic stroke. Acute myocardial infarction （AMI） immediately following t-PA treatment for stroke is a rare but serious complication. A case of acute myocardial infarction （MI） following IV t-PA infusion for acute stroke was observed. This is a 52-year-old male with a known history of hypertension and chest pain, who subsequently developed MI four hours after IV t-PA was administered for acute ischemic stroke. The disruption of intra-cardiac thrombus and subsequent embolization to the coronary arteries may be an important mechanism. In addition, spontaneous recanalization of infarct-related arteries may be associated with greater myocardial salvage and better prognosis.

关键词： stroke myocardial infarction tissue plasminogen activator

Effects of nitrogen application rate and hill density on rice yield and nitrogen utilization in sodic saline–alkaline paddy fields

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Journal of Integrative Agriculture 2021年第2期20卷 540-553页

作者： GUO Xiao-hong lan Yu-chen XU Ling-qi YIN Da-wei LI Hong-yu QIAN Yong-de ZHENG Gui-ping LU Yan-dong Heilongjiang Provincial key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement/Daqing key Laboratory of Saline-alkaline land Utilization and Improvement/College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqing 163319P.R.China

Soil salinity and alkalinity can inhibit crop growth and reduce yield,and this has become a global environmental *** changes in nitrogen (N) application and hill density can improve rice yields in sodic saline–alkaline paddy fields and protect the *** investigated the interactive effects of N application rate and hill density on rice yield and N accumulation,translocation and utilization in two field experiments during 2018 and 2019 in sodic saline–alkaline paddy *** N application rates (0 (control),90,120,150,and 180 kg N ha^(-1) (N0–N4),respectively) and three hill densities(achieved by altering the distance between hills,in rows spaced 30 cm apart:16.5 cm (D1),13.3 cm (D2) and 10 cm (D3))were utilized in a split-plot design with three *** application rate and hill density significantly affected grain *** mathematical model of quadratic saturated D-optimal design showed that with an N application rate in the range of 0–180 kg N ha^(-1),the highest yield was obtained at 142.61 kg N ha^(-1) which matched with a planting density of 33.3×10^(4) ha^(-1).Higher grain yield was mainly attributed to the increase in panicles m^(–2).Nitrogen application rate and hill density significantly affected N accumulation in the aboveground parts of rice plants and showed a highly significant positive correlation with grain yield at *** full heading to maturity,the average N loss rate of the aboveground parts of rice plants in N4 was 70.21% higher than that of *** is one of the reasons why the yield of N4 treatment is lower than that of the N3 *** accumulation rates in the aboveground parts under treatment N3 (150 kg N ha^(-1)) were 81.68 and 106.07% higher in 2018 and 2019,respectively,than those in the *** N translocation and N translocation contribution rates increased with the increase in the N application rate and hill density,whereas N productivity of dry matter and grain first increased

关键词： rice yield saline–alkaline soil nitrogen accumulation paddy field Songnen Plain

非离子微乳液制备及其对钻井液堵塞的解除作用

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钻井液与完井液 2016年第3期33卷 1-6页

作者：蓝强中石化胜利石油工程有限公司钻井工艺研究院山东东营257017

针对钻井过程中存在的固相堵塞、水锁、粗乳液、近井壁地带润湿性等伤害严重问题,基于乙二醇辛基苯基醚的非离子微乳液,对微乳液的性能进行了表征,并考察了微乳液对钻井液堵塞的解除作用。研究表明,研制的微乳液具有界面张力小于0.1 m ... 详细信息

针对钻井过程中存在的固相堵塞、水锁、粗乳液、近井壁地带润湿性等伤害严重问题,基于乙二醇辛基苯基醚的非离子微乳液,对微乳液的性能进行了表征,并考察了微乳液对钻井液堵塞的解除作用。研究表明,研制的微乳液具有界面张力小于0.1 m N/m、可快速增溶高黏原油,pH值、离子类型、矿化度和温度对其增溶能力影响小等特点。实验结果表明,微乳液可有效清除乳液堵塞,清除泥饼中的原油分子,使孔隙的润湿性发生反转,从而恢复储层渗透率;微乳液解堵效率高,泥饼清除效率大于90%,渗透率恢复值在95%以上。

关键词：非离子微乳液钻井液增溶解堵储层保护

A Multi-event Extraction Model for Nursing Records

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国际计算机前沿大会会议论文集 2022年第2期 146-158页

作者： Ruoyu Song lan Wei Yuhang Guo School of Computer Science and Technology Beijing Institute of TechnologyBeijing 100081China Xuanwu Hospital Capital Medical University Beijing 100053China

Nursing records contain information on patients’treatment processes,which reflect the changes in patients’conditions and have legal ***,some of the written records of intensive care unit(ICU)nurses are incomplete according to our *** paper proposes an approach extracting structured nursing events from unstructured nursing records for detecting the missing items *** to the PIO(problem,intervention,outcome)principle in the field of medical care,we propose event schemas for nursing records and annotate a Chinese nursing event extraction dataset(CNEED)on ICU nursing *** find that several events may occur in a nursing ***,we present a multi-event extraction model for the nursing *** experimental results demonstrate that our model achieves good results on CNEED and outperforms competitive methods on the multi-event argument attribution *** observing the results of automatic event extraction by our model,we detect missing items in the existing nursing *** proves that our model can be used to help nurses check and improve the method of recording nursing processes.

关键词： Event extraction Nursing records Multi-event

Free and Hidden Fumonisins in Hordei Fructus Germinatus and Their Transfer to the Decoction

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World Journal of Traditional Chinese Medicine 2020年第1期6卷 106-111页

作者： Ling Hu Hai-Qiang Zhu Mei-Lin Ni Jian Yang Chuan-Zhi kang lan-Ping Guo Ningbo Academy of Inspection and Quarantine China Academy of Chinese Medical SciencesBeijingChina Ningbo National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingChina

Objective:This study aimed to determine the free and hidden fumonisins in Hordei Fructus Germinatus samples and to investigate the transfer rates of these mycotoxins from Hordei Fructus Germinatus to its *** and Methods:The contamination levels of free and hidden fumonisins in a total of 60 Hordei Fructus Germinatus samples were analyzed using high-performance liquid chromatography coupled to tandem mass spectrometry(LC-MS/MS).The decoction procedure was simulated with a highly contaminated Hordei Fructus Germinatus sample,and fumonisins in the resulting decoction preparation were determined by LC-MS/***:Among all the samples,8.3%were contaminated with free fumonisins(FB1 and/or FB2)and 13.3%were contaminated with total fumonisins(free+hidden,measured as hydrolyzed fumonisins,i.e.,HFB1 and/or HFB2).The concentrations of FB1 and HFB1 reached up to 83 and 95μg/kg,respectively,whereas FB2 and HFB2 were detected only in *** transfer rates of free and total fumonisins from Hordei Fructus Germinatus to the decoction were 71.8%and 83.3%for FB1 and FB2,*** comparison,much lower transfer rates were found for total fumonisins,i.e.,38.2%and 24.7%for HFB1 and HFB2,***:The incidence and contamination levels of free and hidden fumonisins in Hordei Fructus Germinatus samples were generally *** decoction preparation,the transfer rates of free fumonisins into the decoction were high,whereas a large part of hidden fumonisins were retained in Hordei Fructus Germinatus rather than migrating into water.

关键词： Decoction fumonisins hidden fumonisins Hordei Fructus Germinatus liquid chromatography coupled to tandem mass spectrometry transfer rate

Development and validation of a risk prediction model for repeated indwelling urinary catheterization in patients with cervical cancer after surgery

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Clinical Research Communications 2023年第1期6卷 19-24页

作者： Fang Wang Xiao-Li Wang Shi-Qun Zhou lan-lan Lou Zhi-Min Wu Department of Gynecology and Obstetrics Third Military Medical University Southwest HospitalChongqing 404100China Department of Geriatrics The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 404100China

Objective:To study the factors influencing secondary indwelling catheterisation after cervical cancer surgery and to develop a predictive risk ***:A total of 260 patients in a tertiary hospital in Chongqing were selected from January 2020 to December 2021 via convenience *** information of patients was recorded,including age;body mass index;history of hypertension and diabetes,bladder dysfunction,postoperative urinary retention,and postoperative urinary tract infection;Histology;staging;surgical approach;Operation time;Time of first remove of catheter;indwelling catheter days;Hospitalization *** absolute shrinkage and selection operator was used to reduce dimensionality and select patient characteristics,and multivariate analysis was performed based on the selected *** on the outcome of analysis,a line chart model was developed for predicting the risk of secondary catheterization in patients with indwelling catheterization after radical cervical cancer *** coefficient of conformity index(C-index)and calibration curves were used to evaluate the accuracy and *** model was internally validated via bootstrapping(1000 random samples),and the clinical utility of the model was assessed via decision curve analysis(DCA).Results:Four characteristic variables were selected,including preoperative bladder function,postoperative urinary tract infection,surgical approach,and Time of first remove of *** are independent risk factors affecting urinary *** risk prediction model exhibited good discrimination performance with a C-index of 0.722(95%CI,0.661-0.783)and was well *** C-index was 0.708 in internal validation *** showed that the risk model was clinically useful for predicting secondary catheterization,and clinical benefits were observed at the decision threshold of≥11%.Conclusion:A novel model was developed to predict the risk of secondary *** model was based on preoperat

关键词： cervical cancer predictors nomogram indwelling catheter secondary catheterization

Genetic Characteristics of Lipooligosaccharide and Capsular Polysaccharide of Campylobacter jejuni from Different Sources in China

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Biomedical and Environmental Sciences 2022年第12期35卷 1106-1114页

作者： WANG Jia Qi CHEN Xiao Li ZHOU Gui lan WANG Hai Rui GU Yi Xin ZHANG Jian Zhong SHAO Zhu Jun ZHANG Mao Jun State key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National Institute of Environmental Health Chinese Center for Disease Control and PreventionBeijing 100021China

Objective To determine the distribution of two important virulence factors[lipooligosaccharide(LOS)and capsular polysaccharide(CPS)]in Campylobacter jejuni(***)isolated from different sources in China and to develop a rapid screening method for Guillain–Barrésyndrome(GBS)-associated *** Whole-genome sequencing was carried out for 494 *** *** Ortho MCL software was used to define the LOS/CPS gene *** genotyping was performed with serotype-specific sequence alignment using the BLAST ***-time Polymerase chain reaction(PCR)was developed with the unique sequences of specific CPS *** Nine novel and 29 previously confirmed LOS classes were *** classes A,B,and C were the most common(48.2%,238/494)among the 494 ***-six capsular types were identified in 448 ***2,HS4c,HS5/31,HS19,and HS8/17 were the most frequent CPS genotypes(58.7%,263/448).Strains of 17 CPS genotypes(strain number>5)had one or two prevalent LOS classes(Pknown *** Our results describe the genetic characteristics of the important virulence factors in *** strains in *** multiplex real-time PCR developed in this study will facilitate enhanced surveillance of GBS-associated strains in China.

关键词： Campylobacter jejuni Lipooligosaccharide Capsular polysaccharide Multiplex real-time PCR

Traveling Wave Solution for Two kinds of Reaction-Diffusion Equations

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Advances in Manufacturing 2000年第2期4卷 108-111页

作者： Jian-lan Hu Han-lin Zhang Beijing Polytechnical University Beijing China

The generialized kuramoto Sivashinski equation and Fisher equation in chemical reaction diffusion was studied in this paper. By introducing a new method, the anthors obtained the exact traveling wave solution for th... 详细信息

关键词： traveling wave solution reaction diffusion equation kuramoto Sivashinski equation Fisher equation