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三种马鲛鱼快速检测方法的建立及应用

食品安全导刊 2024年第7期 67-72页

作者：杨健琪李昂柳淑芳大连海洋大学水产与生命学院辽宁大连116023 中国水产科学研究院黄海水产研究所农业农村部海洋渔业可持续发展重点实验室山东青岛266071

为了准确快速鉴定马鲛鱼样品,本研究分别以线粒体Cytb和D-loop为靶基因,筛选出康氏马鲛、蓝点马鲛与斑点马鲛的特异性PCR扩增引物,建立了3种马鲛鱼的快速检测方法,并对检测方法的特异性、重复性和灵敏性进行了验证,随后运用该方法对市... 详细信息

为了准确快速鉴定马鲛鱼样品,本研究分别以线粒体Cytb和D-loop为靶基因,筛选出康氏马鲛、蓝点马鲛与斑点马鲛的特异性PCR扩增引物,建立了3种马鲛鱼的快速检测方法,并对检测方法的特异性、重复性和灵敏性进行了验证,随后运用该方法对市售马鲛鱼及其加工制品进行检测。结果表明,马鲛鱼的快速检测方法具有良好的特异性和重复性,灵敏性检测限低于2.000 ng·μL^(-1) DNA,运用该方法能快速检测出原料及加工产品中的马鲛鱼成分,整个过程最短用时2 h。本研究建立的3种马鲛鱼PCR快速检测方法,可实现马鲛鱼物种鉴定,满足日常检测需求,对防止水产品掺假、维护消费者权益、规范市场秩序具有重要意义。

关键词：聚合酶链式反应(PCR) 马鲛鱼快速检测物种鉴定

星际分子1-氰基-1,3-丁二烯的气相合成机制研究

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华东师范大学学报（自然科学版） 2024年第4期 57-64页

作者：郑慧敏白西林龚起昂杨涛华东师范大学精密光谱科学与技术国家重点实验室上海200241

为深入了解星际有机腈分子的动力学生成机制,结合同步辐射真空紫外光电离实验和量子化学计算,研究了高温星际环境中氰甲基自由基(·Ch_(2)CN)与丙炔(C_(3)h_(4))的反应机制.通过分析光电离质谱与光电离效率图,发现反应可能主要产生1-氰... 详细信息

为深入了解星际有机腈分子的动力学生成机制,结合同步辐射真空紫外光电离实验和量子化学计算,研究了高温星际环境中氰甲基自由基(·Ch_(2)CN)与丙炔(C_(3)h_(4))的反应机制.通过分析光电离质谱与光电离效率图,发现反应可能主要产生1-氰基-1,3-丁二烯开链异构体.继而,在B3LYP/cc-pVTZ水平上计算了反应势能面,发现氰甲基自由基可无势垒地加成到丙炔上,主要产生gauche-E-1-氰基-1,3-丁二烯和E-1-氰基-1,3-丁二烯这两种产物,而热力学上更稳定的产物吡啶的生成概率较小.

关键词：反应动力学 1-氰基-1,3-丁二烯星际分子

基于CORS网数据的河南“7·20”特大暴雨期间大气水汽分布特征分析

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华北水利水电大学学报（自然科学版） 2024年第1期45卷 21-30页

作者：李慧张亚豪朱丹彤高旭昂华北水利水电大学河南郑州450046

河南“7·20”特大暴雨导致了严重的城市内涝和多起地质灾害。大气水汽是发生极端降雨的主要驱动力之一,分析“7·20”特大暴雨期间大气水汽的时空变化,有助于提高对降雨-水汽区域性作用机制的认识。利用河南CORS网中8个GNSS测站的观测... 详细信息

河南“7·20”特大暴雨导致了严重的城市内涝和多起地质灾害。大气水汽是发生极端降雨的主要驱动力之一,分析“7·20”特大暴雨期间大气水汽的时空变化,有助于提高对降雨-水汽区域性作用机制的认识。利用河南CORS网中8个GNSS测站的观测数据,反演了暴雨前后(7月17—24日)时间分辨率为5 min的PWV数据,在此基础上与基于ERA-5再分析数据通过积分求得的PWV值进行了对比,并结合实际降雨量分析了暴雨期间水汽的时空分布特征。结果表明:GNSS-PWV的偏差和均方根误差分别为0.4、2.3 mm;暴雨前3 d内PWV值呈现快速上升趋势,并在暴雨前3 h达到最大值,在7月22日后逐渐减少到正常范围;GNSS-PWV的变化趋势与实际降雨过程基本符合。研究结果可为基于GNSS观测数据的实时全天候气象预警工作提供一定的理论基础。

关键词： GNSS 大气水汽再分析数据 “7·20”特大暴雨

Precise Rates in the Law of the Logarithm for the Moment Convergence in hilbert Spaces

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Acta Mathematica Sinica,English Series 2009年第2期25卷 191-208页

作者： ke ang FU Li Xin Zhang Department of Mathematics Zhejiang University Hangzhou 310027 P. R. China School of Statistics and Mathematics Zhejiang Gongshang University Hangzhou 310035 P. R. China

Let （X, Xn; n ≥1） be a sequence of i.i.d, random variables taking values in a real separable hilbert space （h, ｜｜·｜｜） with covariance operator ∑. Set Sn = X1 ＋ X2 ＋ ... ＋ Xn, n≥ 1. We prove that, for b 〉 -1, lim ε→0 ε^2（b＋1） ∞ ∑n=1 （logn）^b/n^3/2 E{｜｜Sn｜｜-σε√nlogn}=σ^-2（b＋1）/（2b＋3）（b＋1） B｜｜Y｜^2b＋3holds if EX=0,and E｜｜X｜｜^2（log｜｜x｜｜）^3bv（b＋4）〈∞ where Y is a Gaussian random variable taking value in a real separable hilbert space with mean zero and covariance operator ∑, and σ^2 denotes the largest eigenvalue of ∑.

关键词： the law of the logarithm moment convergence tail probability strong approximation

Amplitude analysis of the decays D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)

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Chinese Physics C 2024年第8期48卷 6-33页

作者： M.Ablikim M.N.Achasov P.Adlarson O.Afedulidis X.C.Ai R.Aliberti A.Amoroso Q.An Y.Bai O.Bakina I.Balossino Y.Ban h.-R.Bao V.Batozskaya k.Begzsuren N.Berger M.Berlowski M.Bertani D.Bettoni F.Bianchi E.Bianco A.Bortone I.Boyko R.A.Briere A.Brueggemann h.Cai X.Cai A.Calcaterra G.F.Cao N.Cao S.A.Cetin J.F.Chang W.L.Chang G.R.Che G.Chelkov C.Chen C.h.Chen Chao Chen G.Chen h.S.Chen M.L.Chen S.J.Chen S.L.Chen S.M.Chen T.Chen X.R.Chen X.T.Chen Y.B.Chen Y.Q.Chen Z.J.Chen Z.Y.Chen S.k.Choi X.Chu G.Cibinetto F.Cossio J.J.Cui h.L.Dai J.P.Dai A.Dbeyssi R.E.de Boer D.Dedovich C.Q.Deng Z.Y.Deng A.Denig I.Denysenko M.Destefanis F.De Mori B.Fang S.S.Fang W.X.Fang Y.Fang Y.Q.Fang R.Farinelli L.Fava F.Feldbauer G.Felici C.Q.Feng J.h.Feng Y.T.Feng k.Fischer M.Fritsch C.D.Fu J.L.Fu Y.W.Fu h.Gao Y.N.Gao Yang Gao S.Garbolino I.Garzia P.T.Ge Z.W.Ge C.Geng E.M.Gersabeck B.Ding X.X.Ding Y.Ding Y.Ding J.Dong L.Y.Dong M.Y.Dong X.Dong M.C.Du S.X.Du Z.h.Duan P.Egorov Y.h.Fan J.Fang JA.Gilman k.Goetzen L.Gong W.X.Gong W.Gradl S.Gramigna M.Greco M.h.Gu Y.T.Gu C.Y.Guan Z.L.Guan A.Q.Guo L.B.Guo M.J.Guo R.P.Guo Y.P.Guo A.Guskov J.Gutierrez k.L.han T.T.han X.Q.hao F.A.harris k.k.he k.L.he F.h.heinsius C.h.heinz Y.k.heng C.herold T.holtmann P.C.hong G.Y.hou X.T.hou Y.R.hou Z.L.hou B.Y.hu h.M.hu J.F.hu T.hu Y.hu G.S.huang k.X.huang L.Q.huang X.T.huang Y.P.huang T.hussain F.h\"olzken N.h\"usken N.in der Wiesche M.Irshad J.Jackson S.Janchiv J.h.Jeong Q.Ji Q.P.Ji W.Ji X.B.Ji X.L.Ji Y.Y.Ji X.Q.Jia Z.k.Jia D.Jiang h.B.Jiang P.C.Jiang S.S.Jiang T.J.Jiang X.S.Jiang Y.Jiang J.B.Jiao J.k.Jiao Z.Jiao S.Jin Y.Jin M.Q.Jing X.M.Jing T.Johansson S.kabana N.kalantar-Nayestanaki X.L.kang X.S.kang M.kavatsyuk B.C.ke V.khachatryan A.khoukaz R.kiuchi O.B.kolcu B.kopf M.kuessner X.kui A.kupsc W.k\"uhn J.J.Lane P.Larin L.Lavezzi T.T.Lei Z.h.Lei h.Leithoff M.Lellmann T.Lenz C.Li C.Li C.h.Li Cheng Li D.M.Li F.Li G.Li h.Li h.B.Li h.J.Li h.N.Li hui Li J.R.Li J.S.Li k.Li L.J.Li L.k.Li Lei Li M.h.Li P.R.Li Q.M.Li Q.X.Li R.Li S.X.Li T.Li W.D.Li W.G.Li X.Li X.h.Li X.L.Li X.Y.Li Y Institute of high Energy Physics Chinese Academy of SciencesBeijing 100049China Beihang University Beijing 100191China Bochum Ruhr-University D-44780 BochumGermany Budker Institute of Nuclear Physics SB RAS(BINP) Novosibirsk 630090Russia Carnegie Mellon University PittsburghPennsylvania 15213USA Central China Normal University Wuhan 430079China Central South University Changsha 410083China China Center of Advanced Science and Technology Beijing 100190China China University of Geosciences Wuhan 430074China Chung-ang University Seoul06974Republic of Korea COMSATS University Islamabad Lahore CampusDefence RoadOff Raiwind Road54000 LahorePakistan Fudan University Shanghai 200433China GSI helmholtzcentre for heavy Ion Research Gmbh D-64291 DarmstadtGermany Guangxi Normal University Guilin 541004China Guangxi University Nanning 530004China hangzhou Normal University Hangzhou 310036China hebei University Baoding 071002China helmholtz Institute Mainz Staudinger Weg 18D-55099 MainzGermany henan Normal University Xinxiang 453007China henan University Kaifeng 475004China henan University of Science and Technology Luoyang 471003China henan University of Technology Zhengzhou 450001China huangshan College Huangshan 245000China hunan Normal University Changsha 410081China hunan University Changsha 410082China Indian Institute of Technology Madras Chennai 600036India Indiana University BloomingtonIndiana 47405USA INFN Laboratori Nazionali di Frascati INFN Laboratori Nazionali di FrascatiI-00044FrascatiItaly INFN Laboratori Nazionali di Frascati INFN Sezione di PerugiaI-06100PerugiaItaly INFN Laboratori Nazionali di Frascati University of PerugiaI-06100PerugiaItaly INFN Sezione di Ferrara INFN Sezione di FerraraI-44122FerraraItaly INFN Sezione di Ferrara University of FerraraI-44122FerraraItaly Inner Mongolia University Hohhot 010021China Institute of Modern Physics Lanzhou 730000China Institute of Physics and Technology Peace Avenue 54BUlaanbaatar 13330Mongolia Instituto de A

Using e^(+)e^(−)annihilation data corresponding to an integrated luminosity of 2.93 fb^(−1)taken at the center-of-mass energy√s=3.773 GeV with the BESIII detector,a joint amplitude analysis is performed on the decays D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η).The fit fractions of individual components are obtained,and large interferences among the dominant components of the decays D^(0)→a_(1)(1260)π,D^(0)→π(1300)π,D^(0)→ρ(770)ρ(770),and D^(0)→2(ππ)_(S)are observed in both *** the obtained amplitude model,the CP-even fractions of D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η)are determined to be(75.2±1.1_(stat).±1.5_(syst.))%and(68.9±1.5_(stat).±2.4_(syst.))%,*** branching fractions of D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η)are measured to be(0.688±0.010_(stat.)±0.010_(syst.))%and(0.951±0.025_(stat.)±0.021_(syst.))%,*** amplitude analysis provides an important model for the binning strategy in measuring the strong phase parameters of D^(0)→4πwhen used to determine the CkM angleγ(ϕ_(3))via the B^(−)→Dk^(−)decay.

关键词： BESIII D^(0)meson decays amplitude analysis CP-even fraction

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赤子爱胜蚓油脂的提取及脂肪酸组成分析

中国油脂 2024年第7期49卷 118-123,152页

作者：王珂欣贾京华刘薇刘蓬宇张博毅高昂天津中医药大学公共卫生与健康科学学院天津300000 天津民生生物工程股份有限公司天津300000

为促进蚯蚓蛋白粉加工副产物的开发与利用,以赤子爱胜蚓渣料为原料,采用超声辅助水酶法结合酸水解法提取蚯蚓油,研究酶种类、酶解时间和加酶量对蚯蚓油得率的影响,通过正交实验确定最佳酶解条件。同时,对提取的蚯蚓油理化指标及脂肪酸... 详细信息

为促进蚯蚓蛋白粉加工副产物的开发与利用,以赤子爱胜蚓渣料为原料,采用超声辅助水酶法结合酸水解法提取蚯蚓油,研究酶种类、酶解时间和加酶量对蚯蚓油得率的影响,通过正交实验确定最佳酶解条件。同时,对提取的蚯蚓油理化指标及脂肪酸组成进行分析。结果表明:超声辅助水酶法结合酸水解法提取蚯蚓油的最佳酶解条件为使用胃蛋白酶(适宜温度50℃、ph 2.0)、酶解时间2.0 h、加酶量4%,在此条件下蚯蚓油得率为11.45%;蚯蚓原油为浅棕色,稍有浑浊,稍有蚯蚓特有的土腥味,略带有油脂酸败味;蚯蚓油由24种脂肪酸构成,其中饱和脂肪酸含量较高,占总脂肪酸的82.381%,主要为硬脂酸、月桂酸,单不饱和脂肪酸占总脂肪酸的8.565%,主要为油酸、棕榈油酸,多不饱和脂肪酸占总脂肪酸的8.995%,主要为亚油酸,另外蚯蚓油奇数碳脂肪酸含量为13.867%。综上,超声辅助水酶法结合酸水解法对于提取蚯蚓渣料中的油脂有一定效果,具备实际应用的潜力,且得到的蚯蚓油含有丰富的奇数碳脂肪酸,营养价值较高。

关键词：蚯蚓油水酶法提取工艺脂肪酸组成

Validation of CFD-DEM simulation of a liquid-solid fluidized bed by dynamic analysis of time series

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Particuology 2022年第9期20卷 75-87页

作者： Julia Picabea Mauricio Maestri Miryan Cassanello Gabriel Salierno Cataldo De Blasio María angélica Cardona Daniel hojman héctor Somacal Departamento de Industrias Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresCiudad UniversitariaIntendente Güiraldes 2160C1428EGABuenos AiresArgentina CONICET-Universidad de Buenos Aires Instituto de Tecnología de Alimentos y Procesos Químicos-ITAPROQCiudad UniversitariaIntendente Güiraldes 2160 C1428EGABuenos AiresArgentina Faculty of Science and Engineering Abo Akademi UniversityRantakatu 265100 VaasaFinland Laboratorio de Diagnósticos por Radiaciones Dep.Física ExperimentalComisión Nacional de Energía AtómicaBKNA1650 San MartínBuenos AiresArgentina CONICET C1033AAJ Buenos AiresArgentina Escuela de Ciencia y Tecnología Universidad de San MartínBKNA 1650 San MartínBuenos AiresArgentina

Liquid-solid fluidized beds(LSFB)modeling validation is crucial for establishing design rules and monitor-ing ***,it generally relies on comparing global variables,which overlook dynamic features that influence reaction *** work aims to implement time series analysis tools to compare Radioactive Particle Tracking data with a simulation consisting of Computational Fluid Dynamics cou-pled with Discrete-Element *** have been performed in a pilot-scale LSFB of calcium alginate spheres fluidized with a calcium chloride *** Diks'test indicates that the simulation can capture the LSFB *** also allows diagnosing flow regime transitions from the *** of solid dispersion coefficients and mixing times predicted by the simulation are in good agreement with the experiments.

关键词： Liquid-solid fluidization CFD-DEM Dynamic comparison Radioactive particle tracking

Gradient Structured Copper by Rotationally Accelerated Shot Peening

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Journal of Materials Science & Technology 2017年第7期33卷 758-761页

作者： X.Wang Y.S.Li Q.Z.ang Y.h.Zhao Y.T.Zhu Nano Structural Materials Center School of Materials Science and Engineering Nanjing University of Science and Technology Nanjing 210094 China School of Mechanical Engineering Nanjing University of Science and Technology Nanjing 210094 China Department of Materials Science and Engineering North Carolina State University Raleigh NC 27695 USA

A new technology-rotationally accelerated shot peening（RASP）, was developed to prepare gradient structured materials. By using centrifugal acceleration principle and large steel balls, the RASP technology can produce much higher impact energy compared to conventional shot peening. As a proof-of-concept demonstration, the RASP was utilized to refine the surface layer in pure copper（Cu） with an average grain size of 85 nm. The grain size increases largely from surface downwards the bulk, forming an800 ？m thick gradient-structured surface layer and consequently a micro-hardness gradient. The difference between the RASP technology and other established techniques in preparing gradient structured materials is discussed. The RASP technology exhibits a promoting future for large-scale manufacturing of gradient materials.

关键词： Microstructure Rotationally accelerated shot peening Gradient structure hardness Copper

Modeling and flapping vibration suppression of a novel tailless flapping wing micro air vehicle

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Chinese Journal of Aeronautics 2022年第3期35卷 309-328页

作者： Siqi Wang Bifeng SONG ang ChEN Qiang FU Jin CUI School of Aeronautics Northwestern Polytechnical UniversityXi'an 710072China Yangtze River Delta Research Institute of Northwestern Polytechnical University Taicang 215400China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518057China School of Aeronautic Science and Engineering Beihang UniversityBeijing 100083China

This paper establishes and analyzes a high-fidelity nonlinear time-periodic dynamic model and the corresponding state observer for flapping vibration suppression of a novel tailless Flapping Wing Micro Air Vehicle(FWMAV),named ***,a complete modeling of NPU-Tinybird is determined,including the aerodynamic model based on the quasi-steady method,the kinematic and dynamic model about the mechanism of flapping and attitude control,combined with the single rigid body dynamic *** on this,a linearized longitudinal pitch dynamic cycle-averaged model is obtained and analyzed through the methods of neural network fitting and system identification,preparing for the design of flapping vibration suppression *** vibration is an inherent property of the tailless FWMAV,which arises from the influence of time-periodic aerodynamic forces and *** can be captured by attitude and position sensors on the plane,which impairs the flight performance and efficiency of flight controller and *** deal with this problem,a novel state observer for flapping vibration suppression is designed.A robust optimal controller based on the linear quadratic theory is also designed to stabilize the closed-loop *** results are given to verify the performance of the observer,including the closed loop responses combined with robust optimal controller,the comparison of different parameters of observer and the comparison with several classic methods,such as kalman filter,h-infinity filter and low-pass filter,which prove that the novel observer owns a fairly good suppression effect on flapping vibration and benefits for the improvement of flight performance and control efficiency.

关键词： Flapping vibration Flapping wing Linear quadratic theory Robust control State observer