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ROOT EXUDATES FROM CAnOLA EXHIbIT bIOLOGICAL nITRIFICATIOn InHIbITIOn AnD ARE EFFECTIVE In InHIbITInG AMMOnIA OXIDATIOn In SOIL

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Frontiers of Agricultural Science and Engineering 2022年第2期9卷 177-186页

作者： Cathryn A.O'SULLIVAn Elliott G.DUnCAn Margaret M.ROPER Alan E.RICHARDSOn John A.kIRkEGAARD Mark b.PEOPLES CSIRO Agriculture&Food 306 Carmody RdSt LuciaQld 4067Australia CSIRO Agriculture&Food Private Bag 5WembleyWA 6913Australia MbS Environmental 4 Cook StreetWest PerthWA 6005Australia CSIRO Agriculture&Food GPO Box 1700CanberraACT 2601Australia

A range of plant species produce root exudates that inhibit ammonia-oxidizing *** biological nitrification inhibition(bnI)capacity can decrease n loss and increase n uptake from the *** study sought evidence for the existence and magnitude of bnI capacity in canola(brassica napus).Seedlings of three canola cultivars,brachiaria humidicola(bnI positive)and wheat(Triticum aestivum)were grown in a hydroponic *** exudates were collected and their inhibition of the ammonia oxidizing bacterium,nitrosospira multiformis,was *** pot experiments were used to test the inhibition of native nitrifying communities in *** exudates from canola significantly reduced nitrification rates of both *** cultures and native soil microbial *** level of nitrification inhibition across the three cultivars was similar to the well-studied high-bnI species *** capacity of canola may have implications for the n dynamics in farming systems and the n uptake efficiency of crops in rotational farming *** reducing nitrification rates canola crops may decrease n losses,increase plant n uptake and encourage microbial n immobilization and subsequently increase the pool of organic n that is available for mineralization during the following cereal crops.

关键词： ammonia oxidizing microorganisms biological nitrification inhibition farming rotations nitrogen cycling nitrogen use efficiency

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MR弹性成像早期检测肥胖大鼠脂肪性肝炎

国际医学放射学杂志 2009年第6期 583-584页

作者： n.Salameh b.Larrat J.A.Quinones S.Pallu M.Dorvillius I.Leclercq 苏晨 Diagnostic Radiology Unit Universite' Catholique de LouvainSt-Luc University HospitalAvenue Hippocrate 10BrusselsBelgium

目的评估MR弹性成像检测肥胖大鼠非酒精性脂肪性肝炎的潜在价值。材料与方法本研究获得当地伦理委员会的批准。54只大鼠分组,分别施行标准饮食喂养、无胆碱饮食喂养直至8周诱发脂肪性肝炎、乳清酸饮食喂养2周诱发肝脏脂肪变性及注射四氯化

关键词：弹性成像 MR 肥胖大鼠脂肪早期检测肝炎肝疾病

CD300a inhibits CD16-mediated nk cell effector functions in HIV-1-infected patients

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Cellular & Molecular Immunology 2019年第12期16卷 940-942页

作者： Joana Vitallé Iñigo Terrén Ane Orrantia Raquel Pérez-Garay Francesc Vidal JoséA.Iribarren Carmen Rodríguez Ana M.López Lirola Enrique bernal Olatz Zenarruzabeitia Francisco borrego Immunopathology Group Biocruces Bizkaia Health Research InstituteBarakaldoSpain Hospital Universitari de Tarragona Joan XXIII IISPVUniversitat Rovira i VirgiliTarragonaSpain Servicio de Enfermedades Infecciosas Hospital Universitario DonostiaInstituto de Investigación Sanitaria BiodonostiaDonostia-San SebastiánSpain Centro Sanitario Sandoval Hospital Clínico San CarlosInstituto de Investigación Sanitaria San CarlosMadridSpain Hospital Universitario de Canarias Santa Cruz de TenerifeSpain Department of Clinical Medicine Universidad de Murcia and Hospital General Universitario Reina SofíaMurciaSpain Ikerbasque Basque Foundation for ScienceBilbaoSpain

natural killer(nk)cell-mediated antibody-dependent cellular cytotoxicity(ADCC)through CD16 plays a critical role in antihuman immunodeficiency virus(HIV)responses.1–3 CD300a is a surface receptor highly expressed on nk cells that has the capacity to inhibit nk cell-mediated cytotoxicity in healthy donors.4 The CD300a molecule has been related to several viral infections and is able to diminish the nk cell killing of pseudorabies-infected cells through interactions with its ligands phosphatidylserine and phosphatidylethanolamine.5 In addition,CD300a expression on b and CD4+T lymphocytes is altered during HIV-1 infection,and combined antiretroviral therapy(cART)does not restore nonpathological expression levels.5,6 However,the expression and function of CD300a on nk cells during HIV-1 infection is still *** have determined the surface expression of CD300a on different nk cell subsets and the capacity of this receptor to inhibit CD16-induced nk cell effector functions in healthy and HIV-1 infected individuals.

关键词： CD16 CD30 ADCC

Feeding Value of Cornflakes Waste Meal on the Performance and Apparent nutrient Digestibility of Starting broilers

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Journal of Agricultural Science and Technology(A) 2011年第5期1卷 722-727页

作者： S. O. Omoikhoje C. O. n. Ekejiuba Department of Animal Science Ambrose Alli University P.M.B. 14 Ekpoma 310001 Edo State Nigeria

One hundred and twenty day-old Anak 2,000 broilers were used to test the effect of replacing maize with cornflakes waste meal （CFWM） on their performance and apparent nutrient digestibility in a complete randomized design. Five dietary, treatments （ l, 2, 3, 4 and 5） were lbnnulated and CFWM was included in the diet in place of maize at concentrations of 0, 25, 50, 75 and 100% respectively. The results revealed that dietary, inclusion of CFWM from 25%-75% significantly （P 〈 0.05） increased average live weight and weight gain, bnt reduced the teed intake and improved the feed to gain ratio of birds compared to those on the control diet （0% CFWM）. besides, apparent digestible crude fibre, ether extract and ash of broilers on the control diet were lower （P 〈 0.05） thm~ those on CFWM based diets. The overall results show that CFWM can successfully replace maize tip to 75% levels as all energ） source with significant improvement in growth rate and nutrient retention of broiler starters.

关键词： broilers cornflakes wastes nutrient digestibility performance.

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肿瘤的发生:射频消融治疗的“脱靶”效应

国际医学放射学杂志 2015年第5期 480-481页

作者： n.Rozenblum E.Zeira V.Scaiewicz b.bulvik S.Gourevitch H.Yotvat 张坤 Goldyne Saved Institute for Gene Therapy and Department of Radiology Hadassah Hebrew University Medical Center Israel and Laboratory for Minimally Invasive Tumor Therapies Department of RadiologyBeth Israel Deaconess Medical CenterHarvard Medical School

摘要目的采用慢性炎症背景下自发形成HCC的小鼠模型(即MDR2敲除模型)比较肝细胞癌(HCC)在射频消融治疗、外科局部肝切除和假手术后的发展情况并抑制HCC射频消融术后复发。材料与方法动物实验经实验动物管理委员会批准并按协议实施。采用... 详细信息

摘要目的采用慢性炎症背景下自发形成HCC的小鼠模型(即MDR2敲除模型)比较肝细胞癌(HCC)在射频消融治疗、外科局部肝切除和假手术后的发展情况并抑制HCC射频消融术后复发。材料与方法动物实验经实验动物管理委员会批准并按协议实施。采用kaplan-Meier生存曲线分析比较了实施射频消融治疗、35%部分肝切除(即肝左叶切除)

关键词：射频消融治疗手术 MDR 敲除肝切除 HCC 肿瘤负荷

人骨髓基质细胞定向培养为内皮细胞应用于心脏组织工程瓣膜

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中国组织工程研究与临床康复 2007年第2期11卷 264-267,T0002页

作者：冯滨刘迎龙谢宁朱晓东晏明全阮英卯川北医学院附属南充市中心医院心胸外科四川省南充市637000 中国医学科学院阜外心血管病北京市100037

目的:观察在脱细胞生物瓣支架上种植自体内皮细胞后完成体外构建生物组织工程瓣的可行性。方法:实验于2002-12/2004-12在中国医学科学院阜外心血管病医院先心病研究室和解放军军事医学科学院全军造血干细胞中心实验室完成。①骨髓样品... 详细信息

目的:观察在脱细胞生物瓣支架上种植自体内皮细胞后完成体外构建生物组织工程瓣的可行性。方法:实验于2002-12/2004-12在中国医学科学院阜外心血管病医院先心病研究室和解放军军事医学科学院全军造血干细胞中心实验室完成。①骨髓样品取自军事医学科学院附属医院全军造血干细胞移植中心的健康成人献髓员,从正常成人骨髓中分离骨髓基质细胞进行定向培养和扩增其中的内皮细胞。②十二烷基硫酸钠脱去成人主动脉带瓣管道表面的内皮细胞后作为支架材料;脱细胞的同种生物瓣支架在无菌条件下,剪切成0.8cm×0.8cm的大小,种子细胞选择人骨髓基质细胞体外定向培养的内皮细胞,高密度(>105cm-2)种植于瓣膜支架上静态培养20d。③分别在静态培养的第7,14,20天结束时取瓣膜片用0.5%硝酸银染色行立体显微镜和扫描电镜观察、摄片。瓣膜被内皮细胞覆盖的面积用百分数表示,以确定其再内皮化率。结果:①5.0×105个骨髓基质细胞体外定向培养的内皮细胞共传8代,最后获得了6.0×109个内皮细胞,扩增了约1.2×104倍,可以满足高密度种植内皮细胞的需求。②0.03%十二烷基硫酸钠完全脱去了同种生物瓣表面的内皮细胞,而瓣膜的细胞外基质成份在脱细胞前后均无明显变化,其形态学结构保持良好。③内皮细胞与支架复合体静态培养第7,14,20天,再内皮化率分别为65%、85%和90%。结论:骨髓基质细胞定向培养的内皮细胞,经扩增后是瓣膜组织工程研究中种子细胞的又一来源;静态培养条件下基本实现了生物组织工程瓣体外构建的目标,为进一步脉动流培养以及动物实验提供了材料基础。

关键词：心脏瓣膜组织工程骨髓基质细胞内皮细胞支架

High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155b1

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The Crop Journal 2023年第6期11卷 1852-1861页

作者： Jian Wang Hongyu Li Tao Shen Shikai Lyu Shams ur Rehman Hongna Li Guiping Wang binyang Xu Qing Wang Wanyi Hu kairong Li Shengsheng bai Jian Ma Haitao Yu Matthew n.Rouse Shisheng Chen School of Advanced Agriculture Sciences Peking UniversityBeijing 100871China national key Laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural SciencesShandong Laboratory of Advanced Agricultural Sciences in WeifangWeifang 261325ShandongChina Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Wheat Research Institute Weifang Academy of Agricultural SciencesWeifang 261071ShandongChina US Department of Agriculture-Agricultural Research Service Cereal Disease Laboratory and Department of Plant PathologyUniversity of MinnesotaSt.PaulMN 55108USA

Stem rust,caused by Puccinia graminis ***(Pgt),threatens global wheat *** of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the *** this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155b1 from the durum wheat line *** seedling tests of biparental populations,Sr8155b1 was effective against six Chinese Pgt races *** a segregating population of 5060 gametes,Sr8155b1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference *** regions include several typical nucleotide-binding leucine-rich repeat(nLR)and protein kinase genes that represent candidate *** them,three nLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line *** markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155b1 absence or presence in 78 tetraploid wheat genotypes *** presence of Sr8155b1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)*** genetic map and linked markers developed in this study may accelerate the deployment of Sr8155b1-mediated resistance in wheat breeding programs.

关键词： Durum wheat Stem rust Resistance gene Sr8155b1 CC-nbS-LRR

Two options for Tele-echography:(a)a robotic arm,ISDn or Satellite lines,(b)a volumic echographic capture system,and internet(Fetal and Abdominal application)

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Two options for Tele-echography:(a)a robotic arm,ISDN or Sat...

nonfunctional ingestion of plant miRnAs in silkworm revealed by digital droplet PCR and transcriptome analysis

庆祝中国超声诊断50年暨第十届全国超声医学学术会议

作者： Ph Arbeille F Cloppet A boucher A Capri n Vincent Medecine Physiologic Spatiale EFMP CHU Trousseau-37044-Tours-France

Objective:to test(a)the tele-echography in realtime based on the use of a robotic arm,(b)the delayed tele echography using a volumic echographic capture and delayed processing Method:A dedicated robotic arm(ESTELE)holding a real ultrasound probe is remotely controlled from the expert site with a fictive probe,and reproduces on the real probe all the movements of the expert hand A dedicated motorized probe holder(TIITER)was used for tIIting any 2D probe from5 to+45。Results:During fetal robotized Tele-echography the expert was able to visualize and measure the fetal structures in 95% of the cases,whlle during abdomen echography the expert visualized the main orgary and lesions in 87% of the cases The mean duration of the robotized tele echography session for one patient was 16+/-10 *** delayed echography using the TILTER was tested on 50 patients The organs were adequately visualized in 85%of the cases after 3 capture per organ The average time from the first capture untII the diagnostic was delivered was 30+/-10 minutes. Conclusion:Realtime or delayed Tele-echography provide simIIar information as direct examination in at least 85% of the cases no false diagnostic was reported.

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Nonfunctional ingestion of plant miRNAs in silkworm revealed...