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Development and Identification of Novel Rice Blast Resistant Sources and Their Characterization Using Molecular Markers

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Rice science 2015年第6期22卷 300-308页

作者： S.J.S.RAMA DEVI kuldeep SINGH B.UMAkANTH B.VISHALAkSHI P.RENUkA k.vijay sudhakar M.S.PRASAD B.C.VIRAkTAMATH V.RAVINDRA BABU M.S.MADHAV Biotechnology Crop ImprovementIndian Institute of Rice ResearchIndian Council of Agriculture Research School of Agricultural Biotechnology Punjab Agricultural University Plant Pathology Indian Institute of Rice ResearchIndian Council of Agriculture Research

To develop and characterize introgression lines for leaf and neck blast resistance, 326 introgression lines were developed using various accessions of six different AA genome wild species in the genetic background of elite Indian varieties like PRl14 and Pusa 44 and were screened for blast resistance. Stringent phenotyping coupled with genotyping using gene based markers led to the identification of four resistant introgression lines, which showed promising resistance and do not possess any of the tested genes. Furthermore, multi-location screening confirmed the field resistance of the four introgression lines to both leaf and neck blast. Molecular characterization of these introgression lines using genome-wide simple sequence repeat markers revealed the presence of small percentage of wild Oryza genome introgrssion. So these lines can be used for mapping and identification of novel leaf and neck blast resistance genes. Thus, these four introgression lines can be considered as new genetic resources for blast resistance.

关键词： rice blast resistance introgression line gene profiling wild species

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A convenient one-pot three component synthesis of 3-aminoalkylated indoles catalyzed by 3-chlorophenylboronic acid

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Chinese Chemical Letters 2013年第5期24卷 422-424页

作者： Santosh V.Goswami Prashant.B.Thorat vijay N.kadam Sachin A.khiste sudhakar R.Bhusare Department of Chemistry Dnyanopasak College

An efficient protocol was developed for the synthesis of 3-aminoalkylated indoles using 3- chlorophenylboronic acid as a catalyst under ambient temperature *** three-component reaction of indoles,aromatic aldehydes and N-methyl aniline offered corresponding 3-aminoalkylated indoles in excellent *** protocol presents some remarkable features such as mild reaction conditions,simple workup procedure and excellent yields.

关键词： 3-Aminoalkylaed indoles 3-Chlorophenylboronic acid Aromatic aldehyde N-Methylaniline Indoles

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