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Dendritic cell-mimicking scaffolds for ex vivo T cell expansion

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Bioactive Materials 2023年第3期21卷 241-252页

作者： Hye Sung kim Tzu-Chieh Ho Moshe J.Willner Michael W.becker Hae-Won kim kam W.Leong Department of Biomedical Engineering Columbia UniversityNew YorkNYUSA Institute of Tissue regeneration Engineering Dankook UniversityCheonanRepublic of Korea Wilmot Cancer Institute University of Rochester Medical CenterRochesterNYUSA Department of regenerative Dental Medicine College of DentistryDankook UniversityCheonanRepublic of Korea Department of Nanobiomedical Science and Bk21 PLUS NBM Global research Center for regenerative Medicine Dankook UniversityCheonanRepublic of Korea Mechanobiology Dental Medicine research Center Dankook UniversityCheonanRepublic of Korea Department of Systems Biology Columbia University Irving Medical CenterNew YorkNY10032USA

We propose an ex vivo T cell expansion system that mimics natural antigen-presenting cells(APCs)for adoptive cell therapy(ACT).Microfiber scaffolds coated with dendritic cell(DC)membrane replicate physicochemical properties of dendritic cells specific for T cell activation such as rapid recognition by T cells,long duration of T cell tethering,and DC-specific co-stimulatory *** DC membrane-coated scaffold is first surface-immobilized with T cell stimulatory ligands,anti-CD3(αCD3)and anti-CD28(αCD28)antibodies,followed by adsorption of releasable interleukin-2(IL-2).The scaffolds present both surface and soluble cues to T cells ex vivo in the same way that these cues are presented by natural APCs in *** demonstrate that the DC-mimicking scaffold promotes greater polyclonal expansion of primary human T cells as compared toαCD3/αCD28-func-tionalized *** importantly,major histocompatibility complex molecules derived from the DC membrane of the scaffold allow antigen-specific T cell expansion with target cell-specific killing *** addition,most of the expanded T cells(~97%)can be harvested from the scaffold by density gradient ***,the DC-mimicking scaffold offers a scalable,modular,and customizable platform for rapid expansion of highly functional T cells for ACT.

关键词： Adoptive cell therapy Ex vivo T cell expansion Cytotoxic T cell Dendritic cell membrane Cell membrane coating Scaffold

The Properties and Applications of MoS_2/Ti Composite Coatings in Dry Cutting and Forming

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材料热处理学报 2004年第05B期25卷 894-898页

作者： XiaolingZhang HailinSun D.becker S.Wagner D.G.Teer TeerCoatingsLtd. WestStineHouseBerryHillIndustrialEstateDroitwichWorcestershireUK InstituteforMetalFormingTechnology stuttgartUniversityStuttgartGermany

MoSi/Ti composite coatings (MoST?), have been deposited by a closed-field unbalanced magnetron sputter ion-plating system. The high ion-current densities at low substrate bias voltage of the system ensures that the MoST? coatings are characteristic of dense coherent structures, with high adhesion (critical scratch load above 120 N), high load bearing capacity up to 5 GPa., low friction (0.02-0.1), high wear resistance (less than 4xlO"17 m3/N m) even at a relative humidity of 40%, and high temperature resistance up to 350 °C. The successful applications of MoST? coatings in large- scale productions for dry cutting and forming, with increasing lifetime and productivity are presented.

关键词： MoS2/Ti复合涂层干切割低摩擦力磁控管溅射

A Transcriptome Atlas of Physcomitrella patens Provides Insights into the Evolution and Development of Land Plants

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Molecular Plant 2016年第2期9卷 205-220页

作者： Carlos Ortiz-ramirez Marcela Hernandez-Coronado Anna Thamm Bruno Catarino Mingyi Wang Liam Dolan Jose A.Feijo Jorg D. becker Instituto Gulbenklan de Ciencia Rua da Quinta Grande 6 2780-156 Oeiras Portugal Department of Plant Sciences University of Oxford South Parks Road Oxford OX1 3RB UK Division of Plant Biology The Samuel Roberts Noble Foundation Ardmore OK 73401 USA Department of Cell Biology and Molecular Genetics University of Maryland 0118 BioSclence Research Building College Park MD 20742-5815 USA These authors contributed equally to this article

Identifying the genetic mechanisms that underpin the evolution of new organ and tissue systems is an aim of evolutionary developmental biology. Comparative functional genetic studies between angiosperms and bryophytes can define those genetic changes that were responsible for developmental innovations. Here, we report the generation of a transcriptome atlas covering most phases in the life cycle of the model bryo- phyte Physcomitrella patens, including detailed sporophyte developmental progression. We identified a comprehensive set of sporophyte-specific transcription factors, and found that many of these genes have homologs in angiosperms that function in developmental processes such as flowering and shoot branching. Deletion of the PpTCP5 transcription factor results in development of supernumerary sporangia attached to a single seta, suggesting that it negatively regulates branching in the moss sporophyte. Given that TCP genes repress branching in angiosperms, we suggest that this activity is ancient. Finally, compar- ison of P. patens and Arabidopsis thaliana transcriptomes led us to the identification of a conserved core of transcription factors expressed in tip-growing cells. We identified modifications in the expression patterns of these genes that could account for developmental differences between P. patens tip-growing cells and A. thaliana pollen tubes and root hairs.

关键词： land plant evolution Physcomitrella patens transcriptome atlas TCP branching tip growth

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验证LTE基带接收机

电子技术应用 2009年第10期35卷 4-5页

作者： randy becker 安捷伦科技公司

在传统意义上的无线通信系统中，射频领域的模拟信号可使用模拟技术解调为同相（I）和正交（Q）分量。但在当今软件定义的无线技术时代，中频信号经过变频处理后通常会使用模数转换器（ADC）进行数字化处理，然后再将数字信号馈入基带... 详细信息

在传统意义上的无线通信系统中，射频领域的模拟信号可使用模拟技术解调为同相（I）和正交（Q）分量。但在当今软件定义的无线技术时代，中频信号经过变频处理后通常会使用模数转换器（ADC）进行数字化处理，然后再将数字信号馈入基带进行解调和解码。现在，通过将信号发生器的输出频率设为目标中频来生成恰当的中频（IF）信号，可以在射频领域以外测试基带接收机。

关键词：接收机基带 LTE 验证无线通信系统中频信号模拟信号数字化处理

Positive predictors for gastroesophageal reflux disease and the therapeutic response to proton-pump inhibitors

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World Journal of Gastroenterology 2014年第14期20卷 4017-4024页

作者： Valentin becker Stefan Grotz Christoph Schlag Simon Nennstiel Analena Beitz Bernhard Haller roland M Schmid Alexander Meining Monther Bajbouj Ⅱ. Medizinische klinik und Poliklinik Technische Universit?t München Institut für Medizinische Statistik und Epidemiologie Technische Universit?t München

AIM: To identify objective and subjective predictors for the reliable diagnosis of gastroesophageal reflux disease (GErD) and the response to proton pump inhibitor (PPI) therapy.

关键词： Gastroesophageal reflux Gastroesophageal reflux disease Non-erosive reflux disease Impedance pH measurement Follow-up Therapy Proton pump inhibitor

构词法对无意识加工的影响——中德数字启动效应的比较研究

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心理科学 1993年第1期16卷 8-14页

作者：马红骊方芸秋 G.Luer U.Laβ D.becker r.Heuermann 华东师范大学心理系 Georg August Universitat GottingenGermany

本文采用客观觉察阈值作为启动刺激呈现时间、以数字(阿拉伯数字)和数词(以中、德两种文字写出的数词)为刺激比较研究了在掩蔽刺激条件下中国与德国被试的启动效应.结果表明:1)中国被试的启动效应显著,在德国被试中未发现显著的启动效应... 详细信息

本文采用客观觉察阈值作为启动刺激呈现时间、以数字(阿拉伯数字)和数词(以中、德两种文字写出的数词)为刺激比较研究了在掩蔽刺激条件下中国与德国被试的启动效应.结果表明:1)中国被试的启动效应显著,在德国被试中未发现显著的启动效应;2)除数字—数字刺激组外,中国被试的启动效应显著地大于德国被试;3)中国被试的数词—数字刺激组比其他三个刺激组产生的启动效应大。我们从无意识加工特征、构词法的差异以及并行—连续加工理论的观点对上述结果分别进行了讨论.

关键词：构词法无意识加工启动效应

In-Game Ads Put Brands in Play——How disappearing demographics can be recovered through their video game consoles

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成功营销 2008年第8期 46-48页

作者： Grant Jenman Stephanie becker

Your audience will continue to ***'s that ***'ve already lost young men to digital *** demographics disappear from traditional media every *** the problem gets worse:Married women are spending more time playing online... 详细信息

关键词：营销策略广告企业管理品牌

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Salt Stress Triggers Phosphorylation of the Arabidopsis Vacuolar k＋ Channel TPk1 by Calcium-Dependent Protein kinases （CDPks）

作者： becker等

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Molecular Plant 2013年第4期6卷 1274-1289页

作者： Andreas Latz Norbert Mehlmer Simone Zapf Thomas D. Mueller Bernhard Wurzinger Barbara Pfister Edina Csaszar rainer Hedrich Markus Teige Dirk becker Department of Molecular Plant Physiology and Biophysics Julius-von-Sachs-lnstitute University of Wurzburg Julius-von-Sachs-Platz 2 D-97082 Wurzburg Germany Department of Biochemistry and Cell Biology Max F. Perutz Laboratories University of Vienna Dr. Bohrgasse 9 A-1030 Vienna Austria Present address: Department of Biology I Botany Munich Center for Integrated Protein Science CiPSM Ludwig-Maximilians-Universitat Munchen D-82152 Planegg-Martinsried Germany College of Science King Saud University Riyadh 11451 Saudi Arabia

14-3-3 proteins play an important role in the regulation of many cellular processes. The Arabidopsis vacuolar two-pore k＋ channel 1 （TPk1） interacts with the 14-3-3 protein GrF6 （GF14-λ）. Upon phosphorylation of the putative binding motif in the N-terminus of TPk1, GrF6 binds to TPk1 and activates the potassium channel. In order to gain a deeper understanding of this 14-3-3-mediated signal transduction, we set out to identify the respective kinases, which regulate the phosphorylation status of the 14-3-3 binding motif in TPk1. Here, we report that the calcium-dependent protein kinases （CDPks） can phosphorylate and thereby activate the 14-3-3 binding motif in TPk1. Focusing on the stress-activated kinase CPk3, we visualized direct and specific interaction of TPk1 with the kinase at the tonoplast in vivo. In line with its proposed role in k＋ homeostasis, TPk1 phosphorylation was found to be induced by salt stress in planta, and both cpk3 and tpkl mutants displayed salt-sensitive phenotypes. Molecular modeling of the TPk1-CPk3 interaction domain provided mechanistic insights into TPk1 stress-regulated phosphorylation responses and pinpointed two arginine residues in the N-terminal 14-3-3 binding motif in TPk1 critical for kinase interaction. Taken together, our studies provide evidence for an essential role of the vacuolar potassium channel TPk1 in salt-stress adaptation as a target of calcium-regulated stress signaling pathways involving Ca2＋, Ca2＋-dependent kinases, and 14-3-3 proteins.

关键词： potassium channel vacuole calcium calcium-dependent kinase 14-3-3 protein salt stress.