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Identification of Ca2+ signaling components in neural stem/progenitor cells during differentiation into neurons and glia in intact and dissociated zebrafish neurospheres

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Science China(Life Sciences) 2018年第11期61卷 1352-1368页

作者： Man kit Tse Ting Shing Hung Ching Man Chan Tiffany wong Mike Dorothea Catherine Leclerc Marc Moreau Andrew L.Miller Sarah E.Webb Division of Life Science & State key Laboratory of Molecular Neuroscience HKUSTClear Water BayHongKongChina Centre de Biologie du Developpement(CBD) Centre de Biologie Integrative(CBI)Universite de ToulouseCNRSUPSToulouse F-31062France

The development of the CNS in vertebrate embryos involves the generation of different sub-types of neurons and glia in a complex but highly-ordered spatio-temporal manner. Zebrafish are commonly used for exploring the development, plasticity and regeneration of the CNS, and the recent development of reliable protocols for isolating and culturing neural stem/progenitor cells(NSCs/NPCs) from the brain of adult fish now enables the exploration of mechanisms underlying the induction/specification/differentiation of these cells. Here, we refined a protocol to generate proliferating and differentiating neurospheres from the entire brain of adult zebrafish. We demonstrated via RT-qPCR that some isoforms of ip3 r, ryr and stim are upregulated/downregulated significantly in differentiating neurospheres, and via immunolabelling that 1,4,5-inositol trisphosphate receptor(IP3 R) type-1 and ryanodine receptor(RyR) type-2 are differentially expressed in cells with neuron-or radial glial-like properties. furthermore, ATP but not caffeine(IP3 R and RyR agonists, respectively), induced the generation of Ca^(2+) transients in cells exhibiting neuron-or glial-like morphology. These results indicate the differential expression of components of the Ca^(2+) -signaling toolkit in proliferating and differentiating cells. Thus, given the complexity of the intact vertebrate brain, neurospheres might be a useful system for exploring neurodegenerative disease diagnosis protocols and drug development using Ca^(2+) signaling as a read-out.

关键词： Ca^2+ signaling neurospheres zebrafish neural stem/progenitor cells differentiation IP3receptors ryanodine receptors STIM and Orai

CNEReg Interprets Ruminant-specific Conserved Non-coding Elements by Developmental Gene Regulatory Network

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Genomics, Proteomics & Bioinformatics 2023年第3期21卷 632-648页

作者： Xiangyu Pan Zhaoxia Ma Xinqi Sun Hui Li Tingting Zhang Chen Zhao Nini Wang Rasmus Heller Wing Hung wong Wen Wang Yu Jiang Yong Wang key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Department of Medical Research Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Guangdong Cardiovascular Institute Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Academy of Mathematics and Systems Science Chinese Academy of SciencesBeijing 100190China School of Mathematics University of Chinese Academy of SciencesBeijing 100049China State key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Animal Science and TechnologyGuangxi UniversityNanning 530005China Section for Computational and RNA Biology Department of BiologyUniversity of CopenhagenCopenhagen DK-2100Denmark Department of Statistics Department of Biomedical Data ScienceBio-X ProgramStanford UniversityStanfordCA 94305USA Center for Ecological and Environmental Sciences Northwestern Polytechnical UniversityXi’an 710072China State key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China

The genetic information coded in DNA leads to trait innovation via a gene regulatory network(GRN)in ***,we developed a conserved non-coding element interpretation method to integrate multi-omics data into gene regulatory network(CNEReg)to investigate the ruminant multi-chambered stomach *** generated paired expression and chromatin accessibility data during rumen and esophagus development in sheep,and revealed 1601 active ruminantspecific conserved non-coding elements(active-RSCNEs).To interpret the function of these activeRSCNEs,we defined toolkit transcription factors(TTfs)and modeled their regulation on rumenspecific genes via batteries of active-RSCNEs during *** developmental GRN revealed 18 TTfs and 313 active-RSCNEs regulating 7 rumen functional ***,6 TTfs(OTX1,SOX21,HOXC8,SOX2,TP63,and PPARG),as well as 16 active-RSCNEs,functionally distinguished the rumen from the *** study provides a systematic approach to understanding how gene regulation evolves and shapes complex traits by putting evo-devo concepts into practice with developmental multi-omics data.

关键词： Trait innovation Gene regulatory network Conserved non-coding element Toolkit transcription factor Ruminant

Views and Experience on Patient Engagement in Healthcare Professionals and Patients—How Are They Different?

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Open Journal of Nursing 2017年第6期7卷 615-629页

作者： Eliza L. Y. wong Siufai Lui Annie W. L. Cheung Carrie H. k. Yam Nicole f. Huang Wilson W. S. Tam Engkiong Yeoh The Jockey Club School of Public Health and Primary Care Faculty of Medicine The Chinese University of Hong Kong Hong Kong China Alice Lee Centre for Nursing Studies National University of Singapore Singapore

A patient-centered approach is used to build a therapeutic alliance between patients and the healthcare professionals in care process which should be supported by a good engagement of both parties. The study aimed to explore the gap between healthcare professionals and patients on patient engagement in hospital. It was a cross-sectional survey. 2774 doctors and nurses from Department of Medicine of public hospitals completed the self-administered questionnaire and 1042 patients discharged from corresponding wards completed the telephone interviews. Participants were interviewed using structural questionnaires. The Mann-Whitney test or Pearson’s chi-square test was used to analyze the agreement between health-care professionals and patients on the views and experiences of patient engagement. A difference was considered to be statistically significant when the p-value was f patient engagement, there was a discrepancy on understanding, views and experiences. More healthcare professionals particularly in nursing were concerned about the possible negative impact of the engagement. The majority of healthcare professionals reported that they engaged well with patients, and perceived more difficulties than patients did. The findings highlighted the mutual understanding of patient engagement, involvement and challenges encountered by both groups in Department of Medicine, which was crucial in efforts to provide meaningful patient engagement in regards to jurisdictions, health system, specialty, discipline and background of patients. It provided insight that a collaborative strategy involving both healthcare professional and patients might be an alternative approach to improving patient engagement.

关键词： Patient Engagement Patient Satisfaction Patient Experience Patient Centered Care

Models of Source/Drain Bias on Negative Bias Temperature Instability

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Models of Source/Drain Bias on Negative Bias Temperature Ins...

RANDOMIZED TRIAL COMPARING THE EffECT Of BRIMONIDINE AND TIMOLOL ON VISUAL fIELD LOSS AfTER ACUTE PRIMARY ANGLE CLOSURE

2006 8th International Conference on Solid-State and Integrated Circuit Technology

作者： Z.H.Gan C.C.Liao M.Liao J.P.Wang W.wong B.G.Yan J.f.kang Y.Y.wong Semiconductor Manufacturing International Corporation 18 Zhangjiang RoadPudong New AreaShanghai 201203P.R.China Institute of Microelectronics Peking University

ISBN: (纸本)1424401607

＜正＞This paper discusses the influence of source/drain（S/D） bias on negative bias temperature instability（NBTI） in pMOS *** is found that with the S/D bias increase,the NBTI degradation is initially reduced,but it increases with higher S/D *** models are presented to explain the underlying *** is the graded hydrogen density model,which dominates at the low S/D bias;and the other is the energetic hole model,which successfully explains the high threshold voltage（Vth） shift at high S/D bias.

关键词： Negative bias temperature instability Niobium compounds Titanium compounds Degradation Hydrogen Threshold voltage Plasma temperature Thermal stresses MOSfET circuits CMOS technology

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国际眼科杂志 2003年第1期3卷 77-78页

作者： H.T.wong T.Aung f.T.S.Oen Y.H.Chan B.k.khoo Y.P.Liu C.L.Ho L.H.Thean S.k.L.Seah P.T.k.Chew Tan Tock Seng Hospital Singapore National Eye CentreSingapore National Eye CentreClinical Trails & Epidemiology UnitTan Took Seng HospitalClinical Trails & Epidemiology UnitSingapore National Eye CentreNational University Hospital SingaporeSingapore National Eye CentreNational University Hospital Singapore

Aim: To compare the effect of brimonidine and timolol in reducing visual field loss in acute primary angle closure (APAC) patients. Methods: In addition to standard medical treatment, A-PAC patients were randomised to brimonidine 0.2% or timolol 0.5% at presentation, then twice daily for one month. After laser iridotomy, subjects underwent baseline perimetry during the

关键词：急性原发性闭角型青光眼溴莫尼定噻吗洛尔视野损害随机试验

周围性与中枢性第Ⅵ脑神经麻痹的眼急动的动力学比较

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世界核心医学期刊文摘（眼科学分册） 2006年第9期2卷 7-8页

作者： wong A. M. f. McReelis k. Sharpe J. A. 桑延智(译) Elm Wing S102D Hospital for Sick Children 555 University Avenue Toronto Ont. M5G 1X8 Canada 不详

Objective:To investigate differences between peripheral idiopathic and central sixth nerve palsies from brainstem damage by comparing peak velocities and durations of horizontal ***:fourteen patients with unilateral incomplete sixth nerve palsies caused by idiopathic,presumed ischemic,peripheral damage,5 with incomplete central(fascicular)palsy caused by brainstem lesions,and 10 controls were *** under 1 month in duration were designated as acute and those of longer duration were *** peripheral palsies,five were acute,nine were *** central palsies,two were acute,three were *** made ± 10 deg horizontal saccades while wearing search *** recordings were made in seven patients with acute palsy(five peripheral,two central).Results:Centrifugal abducting saccadic velocities in the paretic eye were subnormal in both central and peripheral acute palsies,as anticipated from lateral rectus *** chronic central palsies,abducting velocities in the paretic eye remained ***,in chronic peripheral palsies,velocities became normal in the tested range of excursion,within 2 months of onset,despite persisting abduction ***:Saccade peak velocities are reduced and their durations are prolonged in the field of action of acutely palsied peripheral and central *** remain reduced in chronic central(fascicular)palsies,consistent with limited regeneration within the *** speeds are repaired in chronic peripheral palsies,probably by remyelination and axonal regeneration,and perhaps also by central monocular adaptation of innervation selectively to the paretic eye,in order to drive both eyes rapidly and simultaneously into the paretic field of motion.

关键词：脑神经麻痹周围性麻痹中枢性动力学急性麻痹脑干损伤外周性眼扫视

Capture and fission analysis of various superheavy isotopes with ACN=258-280

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Communications in Theoretical Physics 2020年第2期72卷 65-74页

作者： Gurjit kaur Manoj k Sharma School of Physics and Materials Science Thapar Institute of Engineering and TechnologyPatiala—147004PunjabIndia

The capture and fission analysis of heavy ion induced fusion reactions leading to the formation of Z=107-111 superheavy nuclei has been carried *** have been made to analyze the synthesis traits,such as excitation functions,formation probabilities,barrier characteristics *** l-summed wong model provides a decent description of available data on capture(σCap)and fusion-fission(σff)cross-sections and hence is exploited to make relevant predictions for future *** capture and fusion-fission excitation functions are predicted for the least explored region of superheavy nuclei(SHN)i.e.Z=*** role of massasymmetry(η),Coulomb factor(ZPZT)deformation and orientations,Businari-Gallone massasymmetry(αBG)fission barrier(Bf)etc is duly *** present study concludes that the mass-asymmetric reactions involving 24Mg,30Si,and 36S projectiles are preferred for the synthesis of unknown isotopes of Z=***,the doubly magic 48Ca-projectile also provides a competing alternative to produce neutron-rich isotopes of the abovementioned SHN.

关键词： Capture and fission cross-section compound nucleus formation probability quasi-fission

外周和中枢性第6对脑神经麻痹的扫视运动比较

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世界核心医学期刊文摘（神经病学分册） 2006年第9期2卷 25-26页

作者： wong A. M. f. McReelis k. Sharpe J. A. 王鹏(译) Elm Wing S102D Hospital for Sick Children 555 University Avenue Toronto Ont. M5G 1X8 Canada 不详

Objective: To investigate differences between peripheral idiopathic and central sixth nerve palsies from brainstem damage by comparing peak velocities and durations of horizontal saccades. Methods: fourteen patients with unilateral incomplete sixth nerve palsies caused by idiopathic, presumed ischemic, peripheral damage, 5 with incomplete central (fascicular) palsy caused by brainstem lesions, and 10 controls were studied. Palsies under 1 month in duration were designated as acute and those of longer duration were chronic. Among peripheral palsies, five were acute, nine were chronic. Among central palsies, two were acute, three were chronic. Subjects made ±10 deg horizontal saccades while wearing search coils. Serial recordings were made in seven patients with acute palsy (five peripheral, two central). Results: Centrifugal abducting saccadic velocities in the paretic eye were subnormal in both central and peripheral acute palsies, as anticipated from lateral rectus weakness. In chronic central palsies, abducting velocities in the paretic eye remained reduced. However, in chronic peripheral palsies, velocities became normal in the tested range of excursion, within 2 months of onset, despite persisting abduction deficit. Conclusions: Saccade peak velocities are reduced and their durations are prolonged in the field of action of acutely palsied peripheral and central nerves. Speeds remain reduced in chronic central (fascicular) palsies, consistent with limited regeneration within the brain. Saccade speeds are repaired in chronic peripheral palsies, probably by remyelination and axonal regeneration, and perhaps also by central monocular adaptation of innervation selectively to the paretic eye, in order to drive both eyes rapidly and simultaneously into the paretic field of motion.

关键词：脑神经麻痹运动速度中枢性扫视周围性麻痹急性麻痹峰值速度外周

活动期葡萄膜炎性青光眼患者接受小梁切除术后的炎症反应及眼压情况（英文）

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国际眼科杂志 2015年第6期15卷 939-943页

作者： Doris W.f.Yick Ho Wing Lau Jacky W.Y.Lee wong C.Y.Chad Can Y.f.Yuen 中国香港明爱医院眼科中国香港玛丽医院眼科

目标:分析活动期葡萄膜炎患者在小梁切除术后的炎症反应及眼压情况。方法:我们回顾了2006-10/2011-03期间,接受了小梁切除术的活动期葡萄膜炎性青光眼患者纪录。手术前后的炎症反应、复发频率、类固醇依赖度及眼压均以配对t检验作比较... 详细信息

目标:分析活动期葡萄膜炎患者在小梁切除术后的炎症反应及眼压情况。方法:我们回顾了2006-10/2011-03期间,接受了小梁切除术的活动期葡萄膜炎性青光眼患者纪录。手术前后的炎症反应、复发频率、类固醇依赖度及眼压均以配对t检验作比较。结果:在29例29眼患者中,90%的患眼在手术时正在接受局部类固醇治疗。患者平均年龄为58.3±14.0a,术前眼压为35.7±8.9mmHg。患者平均复诊时期为35.2±18.7mo。术后3mo前房炎症反应分级平均减少了0.4±0.6(P<0.01)。复发频率在复诊期内每年减少了2.3±2.1次(P<0.01)。术后1a的平均眼压为13.1±4.5mmHg,而其中44.8%患者的眼压无需用药亦能维持在21mmHg或以下。结论:小梁切除术后的葡萄膜炎性青光眼患者在炎症反应及眼压方面均有改善,但成功率较原发性青光眼患者低。小梁切除术可以作为活动期葡萄膜炎性青光眼患者早期控制眼压和炎症反应治疗的一个选择。

关键词：葡萄膜炎小梁切除术青光眼眼压