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Co-Orelation of Histological Dating and Glycogen Content by Histochemical Stain during Various Phases of Menstrual Cycle in Primary Infertility

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Open Journal of Pathology 2013年第2期3卷 65-68页

作者： Anshu Gupta S. k. Mathur anjali Gupta Department of Pathology Institute of Human Behaviour & Allied Sciences (IHBAS) Delhi India

Glycogen and its products of metabolism are considered to be the most important and direct source of nutrient for the early conceptus. The premenstrual biopsies were taken from 100 women with primary infertility whose male partners had normal semenograms. The biopsies were then subjected to Haematoxylin and Eosin (H & E) and Periodic Acid Schiff (PAS) staining with and without diastase to study histological and histochemical changes particularly glycogen content in the endometrium respectively. Histolomorphologic spectrum of endometrium on H & E stained sections revealed early proliferative phase (4%), late proliferative phase (7%) and early secretory phase (7%), mid secretory phase (33%) and late secretory phase (49%). PAS staining with or without diastase for glycogen was performed and glycogen content, as demonstrated by magenta color, was graded from + to ++++. Glycogen content was observed to be “+” to “++” in proliferative phase, where as in secretory phase, it varied from “++” to “+++”. Glycogen was confirmed by treatment with diastase enzyme, after that PAS stain gave negative staining, i.e. loss of magenta color as glycogen was digested by diastase. Glycogen deficiency was observed in 24.7% of secretory phase endometrium of infertile women. Depletion of glycogen results in inadequate preparation of endometrium at the time of implantation, and hence, may be one of the causes of infertility.

关键词： Glycogen Endometrium Primary Infertility

Diabetes Treatment-Related Myths and the Impact of COVID-19 on Diabetes knowledge—An Indian Survey-Based Study

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Journal of Diabetes Mellitus 2023年第2期13卷 130-141页

作者： anjali Vijaya Subhash kumar Jaipur National University Medical College Jaipur Rajasthan Diabetes and Obesity Care Center Boring Canal Road Patna Bihar

Objective: The main aim of the survey was to assess diabetes treatment-related myths prevalent in the Indian population and if COVID-19 pandemic improved their knowledge about diabetes. Results: The survey was completed by 309 participants;66% did not have diabetes. The responses of people with diabetes and those without diabetes were similar. Survey results of the total population showed that the majority believed that diabetes treatment should start early (92.6%);87.4% believed that the treatment should start within three months of diagnosis with modern medicines;67.3% of the participants felt that allopathic medicines for diabetes were safe, 69.6% believed that if started these medications continue lifelong, and 40.5% thought they damaged all major organs. Insulin was thought to be safe by 65% of the surveyed population;60.8% believed that if they started insulin, they would need it life-long;51.5% thought that insulin was started at the last stage of diabetes;and 58.6% believed that insulin caused kidney damage. A total of 58.6% believed that herbal medicines for diabetes were safer than allopathic;76.4% did not believe that the “diabetic cure” shown through television/newspapers was safe and effective;67.3% did not believe that ayurvedic medicines cured diabetes. Of the surveyed population, 67% felt that their knowledge about diabetes improved during the pandemic and 89.3% knew that PWDs have more serious problems with covid infection. Conclusions: Our survey shows that many diabetes treatment-related myths are prevalent in the Indian population even though the COVID-19 pandemic improved their knowledge about diabetes.

关键词： Diabetes Myths Misconceptions Survey Insulin Treatment COVID-19 Barriers

Bacterial Surface Layer Proteins: A Promising Nano-Technological Tool for Bio-Sensing Applications

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Advances in Bioscience and Biotechnology 2019年第3期10卷 42-58页

作者： anjali Sharma Barkha Singhal School of Biotechnology Gautam Buddha University Greater Noida (U.P.) India

The phenomenal rise in the demand of biosensors accelerated their rapid development and immersive applications in the myriads of fields. The essential requirement of developing efficient bio-sensing platform is to find stable well organized interfacial architecture that can serve as an excellent matrix for binding and recognizing biomolecules. In this context, the enormous potential has been envisaged in surface layer proteins that represented themselves as most primitive and simplest self-assembled system with repetitive physicochemical properties for the molecular functionalization of surfaces and various interfaces. The prominence of S-layer proteins has been broadened by integrating genetic engineering approaches for the fine tuning of functional groups and protein domains in geometrically well-defined manner. The efficient and stable binding of various nanomaterials with S-layers in regular arrays has led to paradigmatic shift in their nano-biotechnological sensing applications. More recently, functional S-layer supported lipid membranes have been generated through covalent binding of lipid molecules either with native or recombinant S-layer proteins at nano-scale dimensions serving as “proof of concept” for the development of bio-sensing platform. Thus, in the light of benefits conferred by surface layer proteins for the development of highly efficient biosensors, an exciting path has been opened for broadening their translational applications in drug delivery, disease diagnosis, vaccines development, lab-on-chip devices etc. Therefore, this review intends to describe about the importance of surface layer proteins in the development of biosensors.

关键词： Surface Layer Proteins Nano-Patterning Self-Assembly Liposome Biosensors

Ambient Air Non-Methane Volatile Organic Compound (NMVOC) Study Initiatives in India–A Review

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Journal of Environmental Protection 2011年第1期2卷 21-36页

作者： Amrita Talapatra anjali Srivastava 不详

Different aspects of Volatile Organic Compounds (VOCs) are being investigated in details by different research groups in Indian institutes. The spectrum covers measuring technologies, source apportionment and variability studies, all these are in due process of preparing a guideline for the sustainable development in terms of industrial, infrastructural as well as overall growth of the country. Both the outdoor and indoor air quality has significant impact on human health. With special concentration on BTEX and HAPs, the health related investigations are conducted as part of interdisciplinary studies of environmental science. Newer technologies to remove VOCs under specific industrial and practical conditions are getting emerged as a comparatively new era. It addresses fields like adsorption, condensation, pervaporation, biodegradation and catalytic combustion. Besides, different kinds of biofilters have drawn significant attention nowadays. The final selection of appropriate technology depends on type and concentrations of VOCs, extent of separation required and cost involved. All these technologies are although well studied, but could not be adopted for regular commercial usage till date. There is scope to explore new horizons as well as regular monitoring on the introduced pathways to limit VOC emission in the ambient air. This review aims at a concise discussion on all the areas that come under the umbrella of non-methane VOC technologies.

关键词： VOC India BTEX HAP Biofilters Catalytic Combustion

Transport Properties of the Layer Manganite La1.5Ca1.5Mn2-xFexO7

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World Journal of Condensed Matter Physics 2011年第4期1卷 153-156页

作者： anjali krishnamurthy M. P. Sharma Bipin k. Srivastava 不详

Layered perovskite manganite ceramics with a nominal chemistry La1.5Ca1.5Mn2-xFexO7(x = 0.0, 0.05, 0.1, 0.3, 0.5) were prepared using sol-gel method. The manganese ions are highly mixed states of Mn3+ and Mn4+. It is found that the Mn3+/Mn4+ ratio decreases with the Fe doping content increasing. The conductivity and magnetoresistance (MR) were studied. The sample of x = 0.05 shows metal insulator transition (MIT) at 135 k. The MIT peak temperature (Tp) shifts towards higher temperature with increasing applied magnetic field. All the samples can be well fitted to the variable-range hopping (VRH) model. The maximum value of MR (%) [ρ(0) - ρ(H)]/ρ(0) × 100 for x = 0.05 is 34% (105 k, 7 kOe).

关键词： Layered Perovskite M-I Transition GMR

Detection of Biofilm Forming Bacterial Communities from Urinary Catheter of Patients with Change in Its Antibiotic Susceptibility Pattern and Triclosan Effect from Different Hospitals of Amravati City Maharashtra, India

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Open Journal of Medical Microbiology 2017年第3期7卷 51-66页

作者： anjali A.Tiwari Niraj Ghnawate Department of Microbiology Sant Gadge Baba Amravati UniversityAmravatiIndia

Purpose: Bacterial biofilm develop on the surfaces of urinary catheter and proceed to cause full blown bacterial infections and sepsis. Urinary catheters, infection rates increase with the duration of catheterization at rates of per day with virtually all of those who undergo long-term catheterization becoming infected. Also antibiotics results in the adaptation and development of resistance leading to treatment failure, prolonged hospitalization, increased costs of care, and increased mortality. Methods: In the present study total 200 used urinary catheters were studied from the different hospitals of Amravati city in 2015-2016. Different bacterial uropathogens were isolated by conventional method and biofilm formation was studied by tissue culture plate (TCP). Antibiotic sensitivity was performed by disc diffusion method. Minimum inhibitory concentration (MIC) and Minimum biofilm eradicating concentration (MBEC) of triclosan was determined by TCP. Results: Out of total samples 93% are contaminated. Around 59% urinary catheters contain mixed consortia. Pseudomonas aeruginosa was found to be the strong biofilm forming and multidrug resistant organism. The most effective drug over seven bacteria isolates were chloramphenicol. Triclosan was used to test against the strong and moderate biofilm forming isolates the MIC of triclosan ranged between 1.5 and 1000 μg/ml and MBEC was between 800 and 3200 μg/ml Conclusions: From the study it was concluded that female are more prone to be infected with catheter associated infection. Pseudomonas aeruginosa was found to be deadly caused of infection, as it is highly resistant to antibiotics. Also triclosan showed effective result on the bacterial uropathogens.

关键词： CAUTI Biofilm Uropathogens Antibiotic Susceptibility Triclosan

Effect of PVP, PVA and POLE surfactants on the size of iridium nanoparticles

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Open Journal of Inorganic Chemistry 2012年第3期2卷 67-73页

作者： anjali Goel Neetu Rani Department of Chemistry Kanya Gurukul Mahavidyalaya Gurukul Kangri University Hardwar (U.K) India

Commonly transition metal nano particle are synthesized by physical, chemical or electrochemical methods. In the present work colloidal iridium nanoparticles were synthesized by chemical oxidation method with different surfactants like poly vinyl pyrrolidone (PVP), poly vinyl alcohol (PVA) and poly oxyethylene lauryl ether (POLE). It was found that shape and size of Ir-nano particles resulted were related to kind of capping agent (surfactant) used. The characterization of the synthesized nano particle has been carried out by UV-vis, X-ray diffraction (XRD), FT-IR, scanning electron microscopy (SEM) and transmission electron microscopic (TEM) techniques. UV-vis and FT-IR confirm the oxidation of IrCl3 into IrO2 while XRD confirms the amorphous nature of the iridium nanoparticles synthesized. The morphology and size of the particle were confirmed by TEM. The average particle size determined by Scherrer equation was about 4.12 nm to 4.23 nm with PVP, 2.74 to 3.36 nm with PVA and 20.41 to 42.25 nm with POLE. Poly oxyethylene lauryl ether particles were not further analyzed because of their large size and less stability. Further particle size was confirmed with TEM, which was 4.5 nm with PVP and 7.0 nm with PVA. The particles are spherical with no agglomeration tendency.

关键词： Nano-Materials Surfactant Agglomeration PVP PVA POLE

Effects of lead(Pb)-induced oxidative stress on morphological and physio-biochemical properties of rice

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BIOCELL 2021年第5期45卷 1413-1423页

作者： MURTAZA kHAN IBA NAZAR IBRAHIM AL AZZAWI MUHAMMAD IMRAN ADIL HUSSAIN BONG-GYU MUN anjali PANDE BYUNG-WOOk YUN Laboratory of Plant Functional Genomics School of Applied BiosciencesKyungpook National UniversityDaegu41566South Korea Ministry of Agriculture State Company for Agriculture Supplies Baghdad10064Iraq Laboratory of Crop Physiology School of Applied BiosciencesKyungpook National UniversityDaegu41566South Korea Department of Entomology Abdul Wali Khan University MardanKhyber Pakhtunkhwa23200Pakistan

In rice,high concentration of lead(Pb)can cause phyto-toxicity affecting several physiological *** of rice varieties that are resistant to Pb-induced oxidative stress is an important management strategy in Pb-contaminated *** the current study,we evaluated four different rice cultivars for their response to Pb-induced *** japonica type cultivars X-Jigna,Ediget,and Furat,and one Indica type cultivar Amber 33 were grown in soil containing different Pb concentrations(0 mM,0.6 mM,and 1.2 mM).The soil was treated with 0 mM or 0.6 mM or 1.2 mM Pb solution one month prior to rice seedling ***,four-week-old rice seedlings were transplanted into the treated soil and their responses were observed until *** data revealed that a highest concentration of Pb(1.2 mM)induced significant reduction in agronomic traits such as plant height,number of tillers per plant,number of panicles per plant,and number of spikelets per panicle in all the rice ***,least reduction in the agronomic traits was observed in X-Jigna,whereas the highest reduction in the agronomic traits was observed in *** activity of catalase(CAT),peroxidase(POD),polyphenol oxidase(PPO),and superoxide dismutase(SOD),was evaluated along with the accumulation of superoxide ions(O2.-),protein,proline,chlorophyll,sucrose,glucose,and fructose contents in all the rice cultivars.A significant increase in antioxidant activity and in the accumulation of proline and sucrose contents with the least reduction in the chlorophyll and protein contents was observed in X-Jigna suggesting that X-Jigna is the most tolerant among all the rice cultivars tested against *** the other hand,non-significant and slightly significant increase in the antioxidant activity,less accumulation of proline and sucrose contents,and higher reduction in the chlorophyll and protein contents was observed in Ediget,which further suggest that Ediget is the most s

关键词： Lead(Pb)toxicity Oxidative stress Morphological and physio-biochemical properties of rice

Pharmaceutical amorphous solid dispersion: A review of manufacturing strategies

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Acta Pharmaceutica Sinica B 2021年第8期11卷 2505-2536页

作者： Sonal V.Bhujbal Biplob Mitra Uday Jain Yuchuan Gong anjali Agrawal Shyam karki Lynne S.Taylor Sumit kumar Qi(Tony)Zhou Department of Industrial and Physical Pharmacy College of PharmacyPurdue University Oral Product Development Bristol Myers Squibb Material Science and Engineering Bristol Myers Squibb

Amorphous solid dispersions(ASDs)are popular for enhancing the solubility and bioavailability of poorly water-soluble *** approaches have been employed to produce ASDs and novel techniques are *** review provides an updated overview of manufacturing techniques for preparing *** physical stability is a critical quality attribute for ASD,the impact of formulation,equipment,and process variables,together with the downstream processing on physical stability of ASDs have been *** strategies are proposed to identify suitable manufacturing methods,which may aid in the development of ASDs with satisfactory physical stability.

关键词： Amorphous solid dispersions Stability Drug delivery Manufacturing Solvent evaporation Melting process Co-precipitation Downstream processing Selection criteria