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Efficacy of Bacillus subtilis MBI 600 Against Sheath Blight Caused by Rhizoctonia solani and on Growth and Yield of Rice

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Rice science 2012年第1期19卷 55-63页

作者： k. vijay krishna kumar S. k.. YELLAREDDYGARI M. S. REDDY J. W. k.OEPPER k. S. LAWRENCE X. G. ZHOU H. SUDINI D. E. GROTH S. krishnaM RAJU M. E. MILLER Department of Entomology ＆ Plant Pathology Auburn University Auburn Alabama 36849 USA ＆ Acharya N G Ranga Agricultural University Hyderabad 500030 India Department of Entomology ＆ Plant Pathology Auburn University Auburn Alabama 36849 USA Texas A ＆ M University Agri-Life Research ＆ Extension Center Texas 77713 USA International Crops Research Institute fo)r the Semi-Arid Tropics (ICRISAT) Hyderabad 502324 India Rice Research Station LSU AgCenter LA 70578 USA Andhra Pradesh Rice Research Institute Maruteru AP 534122 India Department of Biological Sciences Auburn University Auburn Alabama 36849 USA

Rice sheath blight disease （ShB）, caused by Rhizoctonia solani, gives rise to significant grain yield losses. The present study evaluated the efficacy of Integral, the commercial liquid formulation of Bacillus subtilis strain MBI 600, against rice ShB and for plant growth promotion. In greenhouse studies, four log concentrations of Integral （from 2.2×10^6 to 2.2×10^9 cfu/mL） were used as seed treatment （ST）. After 25 d, seedlings were dipped （SD） into Integral prior to transplanting. At 30 d after transplanting （DAT）, leaf sheaths were inoculated with immature sclerotia of the pathogen. At 45 DAT, a foliar spray （FS） with Integral was applied to some treatments. The fungicide control was 50% carbendazim at 1.0 g/L, and a nontreated control was also included. Overall, there were 10 treatments, each with five replications. ShB severity was rated at 52 DAT, and seedling height and number of tillers per plant were rated at 60 DAT. In 2009, two field trials evaluated Integral at 2.2×10^8 and 2.2×10^9 cfu/mL. Integral was applied as ST, and seedlings were produced in a nursery bed. After 32 d, seedlings were treated with Integral as SD and transplanted into 10 m^2 block.. Foliar sprays were given at 45 and 60 DAT. There were seven treatments, each with eight replications arranged as a factorial randomized complete block.design. At 20 DAT, the plots were broadcast inoculated with R. solani produced on rice grains. Seedling height before transplanting, ShB severity at 90 DAT, and grain yield at harvest were recorded. Integral at 2.2×10^9 cfu/mL provided significant increase of seedling heights over other treatments under greenhouse conditions. The Integral treatments of ST ＋ SD ＋ FS at 2.2×10^9 cfu/mL significantly suppressed ShB over other treatments. In field studies, Integral provided significant increase of seedling height in nursery, and number of tillers per plant, compared with the control. ShB severity was significantly suppressed with higher concentrations of Integ

关键词： rice sheath blight Rhizoctonia solani plant growth-promoting rhizobacterium Bacillus subtilis

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Magnesium matrix composites for biomedical applications:A review

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Journal of Magnesium and Alloys 2019年第1期7卷 72-79页

作者： vijay kumar Bommala Mallarapu Gopi krishna ChTirumala Rao Department of Mechanical Engineering Acharya Nagarjuna UniversityGuntur-522510Andhra PradeshIndia

In recent years,a new wave of bioactive,biocompatibility and biodegradable metallic materials were developed for orthopedic *** Magnesium,Magnesium alloys,Magnesium alloy-based composites are extensive material to the biomedical applications,by virtue of its high biocompatibility and reasonable *** magnesium,Magnesium alloys can corrode too fast during the physiological conditions and loses their properties before bone *** new era for the development of magnesium-based composites can satisfy the orthopedic ***-based composites,as bio-materials,can produce adjustable mechanical properties lik. Ultimate tensile strength,ductility,elastic modulus,and corrosion resistance in the physiological *** the Mg based composites,the matrix materials are biomedical magnesium alloys base lik. Mg-Ca,Mg-Al,Mg-Zn,and Mg-REE alloy and The reinforcements are based on hydroxyapatite(HAP),calcium polyphosphate(CPP),andβ-tricalcium phosphate(β-TCP)*** comprehensive review is focused on different grades of biodegradable magnesium matrix composites including their mechanical properties and corrosion resistance.

关键词： Magnesium composites Biodegradable Biocompatible Mechanical properties Biological corrosion

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Real Time Implementation of Series Expansion Based Digital Controller for Magnetic Levitation System

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Intelligent Control and Automation 2016年第4期7卷 110-128页

作者： Avadh Pati vijay kumar Verma Richa Negi Shyam krishna Nagar Department of Electrical Engineering Motilal Nehru National Institute of Technology Allahabad India School of Electronics Engineering Kalinga Institute of Industrial Technology Bhubaneswar India Department of Electrical Engineering Indian Institute of Technology BHU Varanasi India

This paper addresses a digital controller for a real time magnetic levitation system using series expansion of pulse transfer function, which achieves desired closed loop response. The proposed digital controller designed, based on series expansion of pulse transfer function by solving a linear equation using the method of least squares, which improves the transient performance and step track.ng capability of the compensated system. The designed algorithm used for the control input is not iterative, so the calculation is very fast. The proposed control scheme has successfully applied on maglev system and also validated through the simulation and hardware experimental results.

关键词： Maglev System Least Squares Series Expansion Pulse Transfer Function Digital Controller

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Late-Season Grass Weed Management with In-Crop and Post-Harvest Herbicides in Twin-Row Glyphosate-Resistant Soybean

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American Journal of Plant Sciences 2015年第1期6卷 213-218页

作者： krishna N. Reddy Charles T. Bryson vijay k. Nandula Crop Production Systems Research Unit USDA-ARS Stoneville Mississippi USA

Emergence of grasses late in the season has become a problem in glyphosate-resistant (GR) soybean production in the southern US. A 3-yr field study was conducted from 2011 to 2013 at Stoneville, MS to determine efficacy of post-harvest and pyroxasulfone-based in-crop herbicides on late-season grasses and yield in twin-row glyphosate-resistant soybean. Experiments were conducted in a split-plot arrangement of treatments in a randomized complete block.design with fall herbicides (with and without pendimethalin at 1.12 k. ai ha-1 and paraquatat 0.84 k. ai ha-1) as main plots and in-crop herbicides as subplots with four replications. The six in-crop herbicide programs were: glyphosate applied early postemergence (EPOST) at 0.84 k.·aeha-1 followed by (fb) glyphosate late postemergence (LPOST) at 0.84 k.·ha-1 with and without pyroxasulfone preemergence (PRE) applied at 0.18 k. ai ha-1, pyroxasulfone PRE fb glyphosate at 0.84 k.·ha-1 LPOST or glyphosate at 0.84 k.·ha-1 + S-metolachlor at 1.68 k. ai ha-1 EPOST, pyroxasulfone PRE fb S-meto- lachlor at 1.12 k.·ha-1 + fomesafen at 0.27 k. ai ha-1 EPOST fb clethodim at 0.14 k. ai ha-1, and a no-herbicide control. Browntop millet, Digitaria spp., and junglerice densities at 2 week. after LPOST, grass weed dry biomass at harvest, and soybean yield were similar regardless of post- harvest herbicides in all three years. At 2 week. after LPOST, browntop millet, Digitaria spp. and junglerice densities were greatly reduced in all five in-crop herbicide treatments compared with no herbicide plot in all three years. Grass weed dry biomass in no-herbicide plots was 3346, 6136, and 6916 k.·ha-1 in 2011, 2012, and 2013, respectively and the five herbicide treatments reduced grass weed dry biomass by at least 87%, 84%, and 99% in 2011, 2012, and 2013, respectively. Soybean yield was higher with all five in-crop herbicide treatments compared to no herbicide control in all three years. These results indicate that browntop millet, Digitaria sp

关键词： Browntop Millet Digitaria spp. Junglerice S-Metolachlor Pyroxasulfone Soybean Twin-Row

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Hyperspectral Imaging for Differentiating Glyphosate-Resistant and Glyphosate-Susceptible Italian Ryegrass

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American Journal of Plant Sciences 2018年第7期9卷 1467-1477页

作者： Yanbo Huang Matthew A. Lee vijay k. Nandula krishna N. Reddy United States Department of Agriculture Agricultural Research Service Stoneville MS USA Electrical and Computer Engineering Mississippi State University Mississippi State MS USA

Glyphosate is widely used in row crop weed control programs of glyphosate-resistant (GR) crops. With the accumulation of glyphosate use, several weeds have evolved resistance to glyphosate. In order to control GR weeds for profitable crop production, it is critical to first identify them in crop fields. Conventional method for identifying GR weeds is destructive, tedious and labor-intensive. This study developed hyperspectral imaging for rapid sensing of Italian ryegrass (Lolium perenne ssp. multiflorum) plants to determine if each plant is GR or glyphosate-susceptible (GS). In image analysis, a set of sensitive spectral bands was determined using a forward selection algorithm by optimizing the area under the receiver operating characteristic between GR and GS plants. Then, the dimensionality of selected bands was reduced using linear discriminant analysis. At the end the maximum lik.lihood classification was conducted for plant sample differentiation of GR Italian ryegrass from GS ones. The results indicated that the overall classification accuracy is between 75% and 80%. Although the accuracy is lower than the classification of Palmer amaranth (Amaranthus palmeri S. Wats.) in our previous study, this study provides a rapid, non-destructive approach to differentiate between GR and GS Italian ryegrass for improved site-specific weed management.

关键词： Hyperspectral Imaging Glyphosate Resistance Italian Ryegrass

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Morpho-Physiological Characterization of Glyphosate-Resistant and -Susceptible Horseweed (Conyza canadensis) Biotypes of US Midsouth

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American Journal of Plant Sciences 2015年第1期6卷 47-56页

作者： vijay k. Nandula Daniel H. Poston Clifford H. k.ger krishna N. Reddy k. Raja Reddy US Department of Agriculture-Agricultural Research Service (USDA-ARS) Crop Production Systems Research Unit Stoneville MS USA Pioneer Hi-Bred International—Southern Business Unit Huntsville AL USA Silent Shade Planting Company Belzoni MS USA Department of Plant and Soil Sciences Mississippi State University Mississippi State MS USA

Horseweed is traditionally considered a non-cropland weed. However, populations resistant to glyphosate have eventually become established in no-till agronomic cropping systems. Growth chamber and greenhouse experiments were conducted to compare selected biological and physiological parameters of glyphosate-resistant (GR) and -susceptible (GS) horseweed biotypes from Mississippi with a broader goal of fitness characterization in these biotypes. Vegetative growth parameters (number of leaves, rosette diameter and area, shoot and root fresh weights) were recorded week.y from 5 to 11 wk.after emergence and reproductive attributes [days to bolting (production of a flowering stalk. and flowering] and senescence were measured for both GR and GS biotypes under high (24°C/20°C) and low (18°C/12°C) temperature regimes, both with a 13-h light period. Physiological traits such as net photosynthesis, phenolic content, and cell membrane thermostability, all in the presence and absence of glyphosate, and leaf content of divalent cations such as Ca2+ and Mg2+ were assayed in the two biotypes under the high temperature regime. All horseweed vegetative growth parameters except root fresh weight were higher in the high temperature regime compared to that in low temperature regime in both biotypes. Number of leaves, rosette diameter and area, shoot and root fresh weight were 40 vs. 35, 9.3 vs. 8.7 cm, 51 vs. 43 cm2, 3.7 vs. 3.2 g, and 3.5 vs. 4.2 g under high and low temperature conditions, respectively, when averaged across biotypes and week.y measurements. All growth parameters listed above were higher for the GR biotype compared to the GS biotype. Number of leaves, rosette diameter and area, shoot and root fresh weight were 38 vs. 37, 9.1 vs. 8.9 cm, 50.2 vs. 44 cm2, 3.9 vs. 3.1 g, and 4.3 vs. 3.5 g for GR and GS biotypes, respectively, averaged across the temperature treatments and week.y measurements. Reproductive developmental data of these biotypes indicated that the GS biotype

关键词： Conyza canadensis Glyphosate Growth Fitness Horseweed Resistance

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Synthetic Work.oad Generation for Cloud Computing Applications

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Journal of Software Engineering and Applications 2011年第7期4卷 396-410页

作者： Arshdeep Bahga vijay krishna Madisetti 不详

We present techniques for characterization, modeling and generation of work.oads for cloud computing applications. Methods for capturing the work.oads of cloud computing applications in two different models - benchmark.application and work.oad models are described. We give the design and implementation of a synthetic work.oad generator that accepts the benchmark.and work.oad model specifications generated by the characterization and modeling of work.oads of cloud computing applications. We propose the Georgia Tech Cloud Work.oad Specification Language (GT-CWSL) that provides a structured way for specification of application work.oads. The GT-CWSL combines the specifications of benchmark.and work.oad models to create work.oad specifications that are used by a synthetic work.oad generator to generate synthetic work.oads for performance evaluation of cloud computing applications.

关键词： Synthetic Work.oad Benchmark.ng Analytical Modeling Cloud Computing Work.oad Specification Language

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Effect of Time of Planting on Cane Yield and Quality Characters in Sweet Sorghum

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Journal of Sustainable Bioenergy Systems 2012年第1期2卷 1-9页

作者： Chamarthy Venk.ta Ratnavathi Sivanuri Ravi kumar Bathula Swaroop vijay kumar Dasari Gopala krishna Jagannath Vishnu Patil Directorate of Sorghum Research (Formerly National Research Centre for Sorghum) Hyderabad India

Sweet sorghum unlik. grain sorghum has potential to accumulate sugars in stalk. similar to sugarcane. Short duration and lower water requirements of sweet sorghum are other advantages over sugarcane. Sorghum is usually grown in k.arif and rabi seasons. As an energy crop, industry demands supply of green cane as raw material through out the year. Hence this agronomic study was conducted to determine suitable time of planting of sweet sorghum under the agro climatic conditions prevailing in India, so that continuous supply of raw material is assured for factory operations through out the year, especially during the lean period of sugarcane crushing. Various physical and chemical characters of plant and stem juice were studied by tak.ng up bimonthly plantings at four locations using 12 genotypes of sweet sorghum in the first year and quarterly plantings at four locations using 10 genotypes (8 genotypes common in both years) in the second year. Of the six plantings, planting during August and April were found to be not suitable for good sweet sorghum yields, therefore, in the second year, four plantings were tak.n up i.e., June, October, December and February at six locations. The various attributes selected for observations include cane yield, percent brix of stem juice, percent juice extractability, percent total soluble sugars and reducing sugars in the stem juice. Highest green cane yield and percent juice extractability was observed in June planting in all genotypes followed by February, and December in both bimonthly and quarterly plantings. Juice extraction percentage was also highest in June plantings followed by October, April and December plantings. June plantings recorded highest percent brix followed by December and February plantings in bimonthly plantings, while in quarterly planting, highest percent brix and total sugars in juice was observed in February planting followed by planting in June.

关键词： Genotypes Date of Planting Biomass Juice Extractability Brix Total Soluble Sugars Reducing Sugars

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一种抗偏头痛复方制剂体外溶出度的比较研究(英文)

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色谱 2010年第1期28卷 93-99页

作者： HIREMATH vijay Basayya k.LIAPERUMAL krishna BHOJRAJ Suresh NANJAN Mulla Joghee TIFAC CORE HD JSS College of PharmacyRocklands JSS University Banim antap

A novel in vitro dissolution profile was developed for formulated drug in combinational form containing naproxen sodium ( NAP) and sumatriptan succinate ( SUMA). This study was performed to understand the dissolution of the drug in the physiological temperature and pH. Dissolution testing was performed using USP 29 type II testing apparatus rotating at 50 r /min,in 900 mL deaerated buffer (pH 1. 2,4. 5 and 6. 8) which was maintained at ( 37 ± 0. 5) ℃. Quantification was performed using a developed and validated high performance liquid chromatographic (HPLC) method. Aceclofenac (ACE) was used as internal standard. SUMA,ACE and NAP w ere eluted at 4. 8,5. 7 and 7. 9 min,respectively. As expected for enteric coated immediate release (IR) tablets,the dissolution of NAP and SUMA was rapid and essentially complete within 2 h using phosphate buffer ( pH 6. 8). The comparison of the dissolution profiles was realized by model independent approach using a difference factor ( f1) ,similarity factor (f2) and dissolution efficiency ( DE). Statistical results show ed the pro-files were similar to the reference and the test products. Hence,this method demonstrated to be adequate for in vitro studies of NAP and SUMA in the combinational dosage form,since there is no official monograph,col-laborating to the official codes.

关键词：甲氧萘丙酸萘普生钠琥珀酸盐分解方法

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Evaluation of Endophytic Bacteria for Plant Growth Promotion and Pathogen Suppression Traits in Saccharum officinarum

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Journal of Agricultural Science and Technology(A) 2017年第8期7卷 537-545页

作者： Penumatsa k.shore Varma k.tam vijay krishna kumar Vanapalli Chandra Sek.ar Dandu Adilak.hmi Madugula Suresh Nambala Raja kumar k.rnam Jayaehandra Ravuri Anitha Department of Plant Pathology Regional Agricultural Research Station Anakapalle 531001 Andhra Pradesh lndia Department of Genetics and Plant Breeding Regional Agricultural Research Station Anakapalle 531001 Andhra Pradesh lndia

Endophytes are beneficial microbes that are capable of promoting growth, besides protecting colonized plants against plant pathogens. These microbes are of either bacterial, fungal or actinomycetes in plants. In the study, the endophytic bacteria isolated from sugarcane with their characterization related to plant growth promotion and pathogen suppression have been reported. Roots, shoots and leaves of rooted tissue culture plantlets of sugarcane cultivars of 87A298 and 2009A107 were excised aseptically and isolated endophytic bacterial strains. The strains were identified using 16S rRNA gene sequence based homology. Molecular characterization of these strains was carried out for presence of antimicrobial genes. The results showed that the endophytes isolated from sugarcane tissue culture plantlets were of the genera Bacillus （B. amyloliquefaciens, B. subtilis, B. cereus, B. safensis, B. siamensis, B. aryabhattai, B. flexus and B. velezensis） and Paenibacillus pabuli. There were three antimicrobial peptides （AMPs） producing genes of bacilysin, bacillomycin and fengycin in B. amyloliquefaciens （SE1, SE7）, B. siamensis （SE4, SE16）, B. subtilis （SE2, SE3） and B. velezensis （SE15）. The biochemical characterization assays showed that some of these strains could produce hydrogen cyanide （HCN）, protease, cellulase and indole acetic acid （IAA）. Few strains （SE1 and SE4） were phosphate solubilizers, whereas nine isolates were found to be diazotrophs. Most of the bacterial isolates were found antagonistic to Fusarium sacchari, the sugarcane wilt pathogen under in vitro conditions. Overall, the results suggested the scope and potentiality of sugarcane endophytic bacteria, isolated from tissue culture plantlets, in promoting plant growth and suppression of sugarcane pathogen.

关键词： Bacillus Fusarium sacchari Paenibacillus antimicrobial peptides.

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