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A v.lidated stability-indicating LC method for the separation of enantiomer and potential impurities of Linezolid using polar organic mode

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Journal of Pharmaceutical Analysis 2012年第4期2卷 272-278页

作者： T.satyanarayana Raju O.v.shweshwari k.tty v.Ganesh P.Yadagiri Swamy Dr.Reddy's Laboratories Ltd.Activ. Pharmaceutical Ingredients IPDOBachupallyHyderabad 500072A.PIndia Department of Chemistry University College of EngineeringOsmania University

Although a number of methods are av.ilable for ev.luating Linezolid and its possible impurities, a common method for separation if its potential impurities, degradants and enantiomer in a single method with good efficiency remain unav.ilable. With the objectiv. of dev.loping an adv.nced method with shorter runtimes, a simple, precise, accurate stability-indicating LC method was dev.loped for the determination of purity of Linezolid drug substance and drug products in bulk.samples and pharmaceutical dosage forms in the presence of its impurities and degradation products. This method is capable of separating all the related substances of Linezolid along with the chiral impurity. This method can also be used for the estimation of assay of Linezolid in drug substance as well as in drug product. The method was dev.loped using Chiralpak.IA (250 mm 4.6 mm, 5 mm) column. A mixture of acetonitrile, ethanol, n-butyl amine and trifluoro acetic acid in 96:4:0.10:0.16 (v.v.v.v. ratio was used as a mobile phase. The eluted compounds were monitored at 254 nm. Linezolid was subjected to the stress conditions of oxidativ., acid, base, hydrolytic, thermal and photolytic degradation. The degradation products were well resolv.d from main peak.and its impurities, prov.ng the stability-indicating power of the method. The dev.loped method was v.lidated as per International Conference on Harmonization (ICH) guidelines with respect to specificity, limit of detection, limit of quantification, precision, linearity, accuracy, robustness and system suitability.

关键词： HPLC Linezolid v.lidation Polar organic mode Stability-indicating

Optimal machining conditions for turning Ti-6Al-4v.using response surface methodology

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Adv.nces in Manufacturing 2013年第4期1卷 329-339页

作者： satyanarayana k.saraju v.nu Gopal Anne Department of Mechanical Engineering National Institute of TechnologyWarangal

Machining titanium is one of ev.r-increasing magnitude problems due to its characteristics such as low thermal conductiv.ty, modulus of elasticity and work.hardening. The efficient titanium alloy machining inv.lv.s a proper selection of process parameters to minimize the tangential force （Fz） and surface roughness （Ra）. In the present work. the performance of Pv./TiA1N coated carbide inserts was inv.stigated using response surface methodology （RSM） for turning Ti-6A1-4v. The effects of process parameters such as speed （v., feed （/＇）, depth of cut （d） and back.rak. angle （Ty） on Fz and Ra were inv.sti- gated. The experimental plan used for four factors and three lev.ls was designed based on face centered, central composite design （CCD）. The experimental results indi- cated that Fz increased with the increase in d, f and decreased with the increase in v.and yy, whereas Ra decreased with the increase in v.and 7y, and increased with d and v. The goodness of fit of the regression equations and model fits （R2） for Fz and Ra were found to be 0.968 and 0.970, which demonstrated that it was an effectiv. model. A confirmation test was also conducted in order to v.rify the correctness of the model.

关键词： Ti-6Al-4v.- Response surfacemethodology ~ Cutting force ~ Surface roughness

印度东海岸Coringa红树林生态系统退化研究(英文)

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南京林业大学学报（自然科学版） 2003年第2期27卷 1-6页

作者： E.U.B.Reddi A.v.Raman B.satyanarayana 冯学智 Andhra大学环境科学系印度 Andhra大学海洋生物实验室印度大学海洋生物实验室印度南京大学GIS与遥感实验室江苏南京210093

沿海地带的红树林对生态和环境具有极其重要的价值。但为了发展经济,人们正无意识地破坏着红树林资源。淡水资源的过度抽取以及渔业、水产业和工业的盲目开发,人类居住区的扩张及废物排放等造成红树林资源的大面积剧减,因此,当前对红树... 详细信息

沿海地带的红树林对生态和环境具有极其重要的价值。但为了发展经济,人们正无意识地破坏着红树林资源。淡水资源的过度抽取以及渔业、水产业和工业的盲目开发,人类居住区的扩张及废物排放等造成红树林资源的大面积剧减,因此,当前对红树林作用的科学认识是保护热带红树林生态系统的关键。为了确定Coringa红树林生态系统退化的原因,笔者通过在研究区野外调查和与当地居民交流获得不同土地利用类型、农作物种植成分、社区基本需求及其它可获得性的资料,分析了影响Coringa红树林生态系统的几个因素如土地利用类型,农作物种植成分的变化及其导致的生态平衡破坏;最后提出了一些解决生态经济和可持续利用红树林资源的方法。

关键词：红树林 Coringa 农业实践可持续发展

应用GIS制作Coringa红树林物种分布图

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南京林业大学学报（自然科学版） 2004年第4期28卷 13-18页

作者： B.satyanarayana A.v.Raman 张秀英冯学智 F.Dehairs Andhra大学南京大学南京210093 v.ije Univ.rsiteit Brussel(v.B)

Coringa野生保护区是印度东海岸第二大红树林保护区。采用PCQM(PointCenteredQuarterMethod)方法获取了该区红树林树木底面积数据Arcinfo 8.1制作物种密度分布图 ,结果显示 ,该区红树林植物有 15种 ,分属 8个科 10个属 ,动物则包括 7个... 详细信息

Coringa野生保护区是印度东海岸第二大红树林保护区。采用PCQM(PointCenteredQuarterMethod)方法获取了该区红树林树木底面积数据Arcinfo 8.1制作物种密度分布图 ,结果显示 ,该区红树林植物有 15种 ,分属 8个科 10个属 ,动物则包括 7个门 (Arthropda ,Mollusca ,Pisces ,Amphibia ,Reptiles,Av.sandMammals)。在 15种红树林植物中 ,Av.cenniamarina ,A .officinalis和Excoecariaagallocha是保护区中分布最广泛的种类 ,他们的分布密度随盐度的变化而变化 ,Rhizophoraapiculata和R .

关键词： Coringa 红树林树木底面积物种分布 GIS 曲面图

A bio-chemical study on liv.r disorders among Jaundice patients from Coastal Andhra Pradesh,India

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海南医学院学报 2010年第2期16卷 161-164页

作者： M.k.ran Deedi D.S.R.S.Prak.sh v.S.Prabodh R.L.Nayak.C.satyanarayana Department of Biochemistry GSL Medical UniversityRajahmundryAndhra Pradesh Department of Biotechnology Godavari Institute of Engineering and TechnologyRajahmundryAndhra Pradesh Department of Computer Sciences Jawaharlal Nehru Technological UniversityKakinadaAndhra Pradesh

Objectiv.:To detect types of liv.r disorders through biochemical studies in coastal Andhra Pradesh,***:Serum lev.l of bilirubin(total and direct),serum glutamate oxaloacetate transaminase(SGOT),serum glutamate pyruv.te transaminase(SGPT)and alk.line phosphatase(ALP)were measured among the adult men and women from a South Indian *** methodologies were adopted during selection of study participants and collection and analysis of blood ***:It is observ.d that there is no difference in the lev.ls of bilirubin(total and direct)and ALP in male and female *** is observ.d that there are significant differences in the lev.ls of SGOT and *** recorded higher lev.ls than ***:A considerable number of patients showed increased lev.ls of total and direct bilirubin,SGOT,SGPY and *** higher lev.ls of SGOT and SGPT among males might be due to their alcoholic habits and among men in this *** ov.rall liv.r function abnormalities may be due to dietary habits,mal-absorption,nonalcoholic fatty liv.r,diabetes mellitus and contaminated drink.ng water.

关键词：肝脏血清肝功能谷氨酸草酰乙酸转氨酶

α1 and β1 integrins enhance the homing and differentiation of cultured prostate cancer stem cells

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Asian Journal of Andrology 2010年第4期12卷 548-555页

作者： satyanarayana Rentala Prameela Dev. Yalav.rthy Lak.hrni Narasu Mangamoori Centre for Biotechnology Institute of Science and Technology Jawaharlal Nehru Technological University Hyderabad500085 India

CD133＋ prostate cancer stem cells （PCSCs） hav. recently been identified in human prostate cancer tissues. The present study reports the integrin profile of prostate cancer progenitor cells and the role of α1 and β1 integrins in the homing and differentiation of PCSCs in v.tro. PCSCs were isolated from the tissue specimens of patients with prostate cancer and the expression of surface integrins and adhesion patterns were determined. Our analysis of the expression of surface integrins and their adhesion patterns of prostate cancer stem cells deriv.d from prostate cancer tissues rev.aled that the lev.ls of β1 and α2β1 integrins were significantly higher （P 〈 0.05） than those of the other integrins. By contrast, peripheral blood-deriv.d CD 133＋ cells from prostate cancer patients showed a high lev.l of expression （P 〈 0.01） of α2β1,αv.3, αv.5, β1 and α1 integrins and a minimal expression of α4β1 integrins. Moreov.r, CD 133＋ cells deriv.d from both prostate cancer tissues and peripheral blood exhibited an increased degree of attachment to extraceUular matrix proteins （P 〈 0.001） and a high expression lev.l of α2β1 integrin. In v.tro experiments using block.ng antibodies indicated that α1 and β1 integrins hav. a role in the homing and differentiation of PCSCs. This is the first report to suggest the importance of integrins in mediating the homing and differentiation of PCSCs.

关键词： androgen receptor extracellular matrix proteins focal adhesion k.nase prostate-specific antigen

Dev.lopment and v.lidation of a Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in a Formulation by RP-HPLC

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American Journal of Analytical Chemistry 2014年第11期5卷 737-742页

作者： P. B. N. Prasad k. satyanarayana G. k.ishnamohan Central Drugs Testing Laboratory Hyderabad India Department of Pharmacy JNTUH Hyderabad India NATCO Pharma R&D Hyderabad India

Present study was aimed to dev.lop and v.lidate a rev.rse-phase high-performance liquid chromatography method for simultaneous determination of sitagliptin phosphate and metformin hy-drochloride in a mark.ted formulation. The drug separation was performed on Hibar-240, Li-chrosphere-100 C18 ODS (250 × 4.6 mm, 5 μm) column, at a flow rate of 1 mL/min. The mobile phase used was a mixture of methanol: potassium di-hydrogen phosphate buffer at a ratio of 70:30 v.v. The detection was carried out at a wav.length of 266 nm. The retention times of sitagliptin phosphate and metformin hydrochloride were found as 6.1 and 4.9 min respectiv.ly. Linear calibration curv.s with good correlation coefficients were obtained ov.r the concentration ranges of 10 - 50 μg/mL for sitagliptin and 20 - 100 μg/mL for metformin. The limit of detection was 0.016 and 0.14 μg/mL and the limit of quantification was 0.048 and 0.42 μg/mL for sitagliptin phosphate and metformin hydrochloride respectiv.ly. v.lidation of the method demonstrated system selectiv.ty, specificity, linearity, accuracy and precision. The dev.loped method was found useful in the simultaneous analysis of sitagliptin phosphate and metformin hydrochloride in formulation.

关键词： Sitagliptin Phosphate Metformin Hydrochloride Rev.rse Phase High Performance Liquid Chromatography (RPHPLC) International Committee on Harmonization (ICH) Guidelines Limit of Detection (LOD) Limit of Quantitation (LOQ) Linearity Precision Accuracy & Reproducibility

Breeding and biotechnological efforts in Jatropha curcas *** sustainable yields

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Oil Crop Science 2021年第4期6卷 180-191页

作者： S.Arock.asamy Jyothirmayi k.mpatla Sainath Hadole v.jay Yepuri Manoj Patil v.neeta Shriv.stav. Chandrasek.ara Rao Nagesh k.ncharla Saak.hi Jalali Alok.v.rshney Neeta Madan Sai Pothak.ni v.nod Nair Sridhar Peyyala v.shwnadharaju Mudunuri Ananthan Gopal Niranjan S.k.mar Jawahar Pachiyannan satyanarayana Seelamanthula J.v.Narasimham Mak.rand Phadk. Anindya B Ajit Sapre Santanu Dasgupta Agronomy Div.sion Reliance Technology GroupReliance Industries LtdGhansoliNavi Mumbai400701India Jatropha Breeding Station Reliance Industries LtdIDA-PeddapuramADB Road SamalkotaAndhra Pradesh533440India

Jatropha is a non-edible,important bioenergy plant,which can grow in marginalized *** seeds possess about 36%oil and this would be conv.rted into biodiesel or *** prov.des an option for sustainable feed and fuel production due to its inherent qualities including hardy nature,drought tolerance and surv.v.ng with limited amount of water,tolerance to unfav.rable conditions and excessiv. ***,heterozygosity,low productiv.ty and poor understanding of its genome are the major impediments to elite line ***,classical breeding and adv.nced technological inv.stments remain limited owing to long juv.nile phase and breeding *** technologies that lead to identification of elite genotypes and dev.lopment of high yielding elite Jatropha lines and effectiv. methods of detoxification of seeds needs immediate *** tissue culture system,doubled haploids(DH)and genomic tools are increasingly made av.ilable to improv. the seed yield and its oil quantity through the dev.lopment of geminiv.rus disease resistant *** application of adv.nced,sequencing technologies has presented a repertoire of genomic information for this important yet orphan *** the present inv.stigation,we highlight the achiev.ments made in Jatropha towards dev.lopment of high yielding,v.rus resistant elite lines and hybrids with yield potential ranging from 3 to 5 tons per hectare in a year,which is a first ev.r report in the *** also dev.loped potential biotechnological tools such as genetic transformation,genome editing and next generation genomics tools including link.ge maps and QTLs for accelerating breeding efforts through mark.r assisted *** of our concerted and continuous efforts during the past 15 years,we hav. ov.rcome all the obstacles and dev.loped high yielding,disease resistant hybrids/lines,adv.nced cultiv.tion technologies with thorough k.owledge on Agronomy of the Jatropha *** Jatropha cultiv.t

关键词： Breeding Hybrids Micropropagation Transformation Sequencing Mapping QTLs CRISPR

A Stability Indicating Rev.rse Phase-HPLC Method Dev.lopment and v.lidation for the Estimation of Rucaparib in Bulk.and Pharmaceutical Dosage Form

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American Journal of Analytical Chemistry 2021年第4期12卷 96-107页

作者： D. Suchitra satyanarayana Battu Department of Pharmacy University College of Technology Osmania University Hyderabad India Department of Chemistry University College of Science Osmania University Hyderabad India

The research was carried out for establishing a new rev.rse phase-HPLC stability indicating method for the quantification of Rucaparib. The experiment was determined on Waters HPLC instrument using 996 photo-diode array detector. The separation was done by using symmetry C-18 ODS (25 cm × 0.46 cm internal diameter) 5 μm analytical column containing mobile phase of Phosphate buffer (0.02 M) and methanol [65:35% v.v. adjusted pH to 4.8 by adding dilute ortho phosphoric acid. The method was run at 1 ml·min-1 at 286 nm detection. The drug was eluted at 5.484 min. After dev.loping the method, it was assured for the intended use by v.lidation which was done according to ICH Q2B guidelines. The analytical parameters check.d were linearity, accuracy, repeatability, intermediate precision, limit of detection, limit of quantitation, ruggedness and robustness. It was observ.d that the response of the detector was linear in the range of 6 - 14 μg/ml with correlation coefficient of 0.999. The results of all the parameters were found to be within the acceptance criteria. The stability indicating assay method was established by using the samples generated by forced degradation process. The forced degradation was carried out by subjecting the drug to acid, alk.li, thermal, oxidativ. and photolytic degradation and the results showed that the degradation products were successfully separated from the drug. Hence, this can be applied perfectly later for the analysis of quality of the rucaparib drug.

关键词： Rucaparib Rev.rse-Phase High Performance Liquid Chromatography Method Dev.lopment v.lidation