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QTL Analysis for Grain Iron and Zinc Concentrations in Two O. nivara Derived Back.ross Populations

Rice science 2018年第4期25卷 197-207页

作者： B. P. M. SWAMY k. kaladhar1, k. ANURADHA Anil k. BATCHU T. LONGVAH N. SARLA Indian Institute of Rice Research Rajendranagar Hyderabad 500030 India Plant Breeding Division International Rice Research Institute DAPO Box 7777 Metro Manila the Philippines International Crops Research Institute on Semi Arid Tropics Patancheru Hyderabad 502324 India National Institute of Nutrition Hyderabad 500007 India

Identification of quantitative trait loci （QTLs） for grain mineral elements can assist in faster and more precise development of micronutrient dense rice varieties through mark.r-assisted breeding. In the present study, QTLs were mapped for Fe and Zn concentrations in two BC2F3 mapping populations derived from the crosses of O. sativa cv Swarna with two different accessions of O. nivara. In all, 10 and 8 QTLs were identified for grain Fe and Zn concentrations in population 1, and 7 and 5 QTLs were identified in population 2, respectively. Eighty percent of the QTLs detected in both populations were derived from O. nivara. Five QTLs for Fe and three QTLs for Zn explained more than 15% phenotypic variance either in interval or composite interval mapping. The locations of O. nivara derived QTLs such as qFe2.1, qFe3.1, qFe8.2 and qZn12.1 were consistently identified in both the populations. Epistatic interaction was observed only between RM106 and RM6 on chromosome 2 and between RM22 and RM7 on chromosome 3 for Fe concentration in population 1. Sixteen candidate genes for metal homeostasis were found to co-locate with 10 QTLs for Fe and Zn concentrations in both the populations. Most of the Fe and Zn QTLs were found to co-locate with QTLs for grain yield and grain quality traits. Some of the major effect QTLs identified can be used to improve rice grain Fe and Zn concentrations.

关键词： biofortification micronutrient Fe concentration Zn concentration rice wild rice quantitative trait locus gene mark.r assisted breeding

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ISSR mark.rs based on GA and AG repeats reveal genetic relationship among rice varieties tolerant to drought,flood,or salinity

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第2期10卷 133-141页

作者： Ch Surendhar REDDY A. Prasad BABU B.P. Mallik.rjuna SWAMY k. kaladhar N. SARLA Biotechnology Laboratory Crop Improvement SectionDirectorate of Rice ResearchRajendranagar

Drought, flood, salinity, or a combination of these limits rice production. Several rice varieties are well k.own for their tolerance to specific abiotic stresses. We determined genetic relationship among 12 rice varieties including 9 tolerant to drought, flood, or salinity using inter-simple sequence repeat （ISSR） mark.rs. Based on all mark.rs, the nine tolerant varieties formed one cluster distinct from the cluster of three control varieties. The salt-tolerant varieties were closest to two flood-tolerant varieties, and together they were distinct from the drought-tolerant varieties. （GA）8YG was the most informative primer, showing the highest polymorphic information content （PIC） and resolving power （Rp）. The drought-, flood-, and salt-tolerant varieties grouped in three distinct clusters within the group of tolerant varieties, when （GA）8YG was used. Sabita was the only exception. The two aus varieties, Nagina22 and FR13A, were separated and grouped with the drought- and flood-tolerant varieties, respectively, hut they were together in dendrograms based on other primers. The results show that ISSR mark.rs associated with （GA）sYG delineated the three groups of stress-tolerant varieties from each other and can be used to identify genes/new alleles associated with the three abiotic stresses in rice germplasm.

关键词： Drought Submergence Salinity Inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) (GA)8YG Nagina22 (N22) FR13A Pok.ali

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Molecular Mapping of QTLs for Yield and Yield-Related Traits in Oryza sativa cv Swarna × O. nivara (IRGC81848) Back.ross Population

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Rice science 2011年第3期18卷 178-186页

作者： B. P. MALLIk.RJUNA SWAMY k. kaladhar M. S. RAMESHA B. C. VIRAk.AMATH N. SARLA Directorate of Rice Research Rajendranagar Hyderabad India - 500030 Plant Breeding Genetics and Biotechnology Division International Rice Research Institute DAPO Box 7777 Metro Manila Philippines

Advanced back.ross QTL analysis was used to identify QTLs for seven yield and yield-related traits in a BC2F2 population from the cross between a popular Oryza sativa cv Swarna and O. nivara IRGC81848. Transgressive segregants with more than 15% increased effect over Swarna were observed for all the traits except days to heading and days to 50% flowering. Thirty QTLs were detected for seven yield and yield-related traits using interval and composite interval mapping. Enhancing alleles at 13 (45%) of these QTLs were derived from O. nivara, and enhancing alleles at all the QTLs for stem diameter and rachis diameter were derived from O. nivara. Three stem diameter QTLs, two rachis diameter QTLs and one number of secondary branches QTL identified by both Interval and composite interval mapping contributed more than 15% of the total phenotypic variance. The QTL epistasis was significant for stem diameter and plot yield. The most significant QTLs qSD7.2, qSD8.1 and qSD9.1 for stem diameter, qRD9.1 for rachis diameter and qNSB1.1 for number of secondary branches are good targets to evaluate their use in mark.r-assisted selection. O. nivara is a good source of novel alleles for yield related traits and reveals major effect QTLs suitable for mark.r-assisted selection.

关键词： Oryza sativa Oryza nivara stem diameter rachis diameter yield yield components quantitative trait locus

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Natural convection flow of a couple stress fluid between two vertical parallel plates with Hall and ion-slip effects

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Acta Mechanica Sinica 2012年第1期28卷 41-50页

作者： D.Srinivasacharya k.kaladhar Department of Mathematics National Institute of Technology

The Hall and ion-slip effects on fully developed electrically conducting couple stress fluid flow between vertical parallel plates in the presence of a temperature dependent heat source are investigated. The governing non-linear partial differential equations are transformed into a system of ordinary differential equations using similarity transformations. The resulting equations are then solved using the homotopy analysis method （HAM）. The effects of the magnetic parameter, Hall parameter, ion-slip parameter and couple stress fluid parameter on velocity and temperature are discussed and shown graphically

关键词： Free convection Couple stress fluid Magneto- hydrodynamics Hall and ion-slip effects - HAM

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Nitric oxide regulation of plant metabolism

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Molecular Plant 2022年第2期15卷 228-242页

作者： k.puganti Jagadis Gupta Vemula Chandra kaladhar Teresa B.Fitzpatrick.Alisdair R.Fernie Ian Max Møller Gary J.Loak. National Institute of Plant Genome Research Aruna Asaf Ali MargNew DelhiDelhi 110067 India Vitamins and Environmental Stress Responses in Plants Department of Botany and Plant BiologyUniversity of GenevaGeneva 1211 Switzerland Max-Planck.Institute of Molecular Plant Physiology Am Mühlenberg 1Potsdam-Golm 14476 Germany Department of Molecular Biology and Genetics Aarhus UniversityForsøgsvej 14200 SlagelseDenmark Institute of Molecular Plant Sciences School of Biological SciencesUniversity of EdinburghEdinburgh EH93BFUK

Nitric oxide(NO)has emerged as an important signal molecule in plants,having myriad roles in plant *** addition,NO also orchestrates both biotic and abiotic stress responses,during which intensive cellular metabolic reprogramming *** to these responses is the location of NO biosynthetic and scavenging pathways in diverse cellular compartments,enabling plants to effectively organize signal transduction *** regulates plant metabolism and,in turn,metabolic pathways reciprocally regu-late NO accumulation and ***,these diverse cellular processes are inextricably *** re-view addresses the numerous redox pathways,located in the various subcellular compartments that pro-duce NO,in addition to the mechanisms underpinning NO *** focus on how this molecular dance is integrated into the metabolic state of the *** this context,a reciprocal relationship be-tween NO accumulation and metabolite production is often *** also showcase cellular pathways,including those associated with nitrate reduction,that provide evidence for this integration of NO function and ***,we discuss the potential importance of the biochemical reactions governing NO levels in determining plant responses to a changing environment.

关键词： hypoxi amitochondria metabolism nitric oxide S-nitrosylation pyridoxine reactive nitrogen species reactive oxygen species

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A Mathematical Study for the Stability of Two Predator and One Prey with Infection in First Predator using Fuzzy Impulsive Control

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Annals of Applied Mathematics 2023年第1期39卷 29-48页

作者： k.ushbu Singh k.lla kaladhar Department of Mathematics National Institute of TechnologyWarangal-506004India

In this study,we develop a set of ordinary differential equations that represents the dynamics of an ecosystem with two predators and one prey,but only the first predator population is affected by an infectious *** Lotk.-Volterra predator-prey system’s model stability have been examined using the Tak.gi-Sugeno(T-S)impulsive control model and the Fuzzy impulsive control *** the formulation of the model,the global stabilities and the Fuzzy solution are carried out through numerical simulations and graphical representations with appropriate discussion for better understanding the dynamics of our proposed model.

关键词： Prey-predator system T-S model stability eco-epidemiology

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Data Categorization and Noise Analysis in Mobile Communication Using Machine Learning Algorithms

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Wireless Sensor Network 2012年第4期4卷 113-116页

作者： Raghavendra Phani k.mar Malleswara Rao Dsvgk.kaladhar Department of Bioinformatics GITAM Institute of Science GITAM University Visakhapatnam India Department of Electronics and Communication Engineering GITAM Institute of Technology GITAM University Visakhapatnam India

Machine learning and pattern recognition contains well-defined algorithms with the help of complex data, provides the accuracy of the traffic levels, heavy traffic hours within a cluster. In this paper the base stations and also the noise levels in the busy hour can be predicted. J48 pruned tree contains 23 nodes with busy traffic hour provided in east Godavari. Signal to noise ratio has been predicted at 55, based on CART results. About 53% instances provided inside the cluster and 47% provided outside the cluster. DBScan clustering provided maximum noise from srik.k.lam. MOR (Number of originating calls successful) predicted as best associated attribute based on Apriori and Genetic search 12:1 ratio.

关键词： Traffic MOR Data Mining

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