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世界有线电视信息 2000年第1期7卷 118-120页

作者： child.,R 休斯网络系统有限公司总经理

Antigen unmask.ng does not improve the visualization of phospholipase C zeta in human spermatozoa

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Asian Journal of Andrology 2022年第4期24卷 345-352页

作者： Xin Meng Celine Jones Pedro Melo Caroline Ross Ginny Mounce Tim child.k.vin Coward Nuffield Department of Women's and Reproductive Health University of OxfordOxford OX39DUUK The Women's Centre Oxford University Hospitals NHS Foundation TrustOxford OX39DUUK xford Fertility Oxford Business Park NorthOxford OX42HWUK.

Phospholipase C zeta(PLCζ)is a sperm-specific protein that triggers oocyte *** analysis of PLCζexpression in human spermatozoa can be used as a diagnostic mark.r for oocyte activation *** laboratory has previously optimized a standard"in-house"assay to determine PLCζexpression in human ***,one study has suggested that an antigen unmask.ng method(AUM)would be more efficient in visualizing PLCζin human *** study aimed to compare our established assay and AUM(involving HCl,acidic Tyrode's solution[AT],and heat).The mean relative fluorescence(RF)intensity of PLCζin frozen-thawed spermatozoa from fourteen fertile donors stained with the in-house method was significantly higher than three other AUM groups(in-house[mean±standard error of mean]:18.87±2.39 arbitrary units[a.u.]vs non-AUM:11.44±1.61 a.u.,AT-AUM:12.38±1.89 a.u.,and HCl-AUM:12.51±2.16 a.u.,P<0.05,one-way analysis of variance).The mean RF intensity of PLCζin AT-and HCl-treated spermatozoa from 12 infertile males was not significantly different from that of the non-AUM ***,the in-house method resulted in the highest RF intensity(12.11±1.36 a.u.,P<0.01).Furthermore,specificity testing of antibody-antigen binding indicated that the in-house method showed more specific binding than spermatozoa treated by the *** conclusion,our in-house method showed superior visualization and reliability than the AUM,thus supporting the continued use of our in-house assay for clinical research screening.

关键词： antigen unmask.ng immunofluorescence staining male infertility phospholipase C zeta

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Auditory steady-state evok.d response in diagnosing and evaluating hearing in infants

作者： child.k.

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Neural Regeneration Research 2006年第9期1卷 825-828页

作者： Fei Mai1, Xiaozhuang Zhang1, Qunxin Lai1, Yanfei Wu1, Nanping Liao2, Yi Ye3, Zhenghui Zhong4 1Auditory Center, Guangdong Maternal and child.Health Care Hospital, Guangzhou 510010, Guangdong Province, China 2Department of child.Health Care, Shunde Maternal and child.Health Care Hospital, Shunde 528300, Guangdong Province, China 3Department of child.Health Care, Nanhai Maternal and child.Health Care Hospital, Nanhai 528200, Guangdong Province, China 4Department of child.Health Care, Huadu District Maternal and child.Health Care Hospital, Guangzhou 510800, Guangdong Province, China Auditory Center Guangdong Maternal and Child Health Care Hospital Guangzhou 510010 Guangdong Province China Department of child.Health Care Shunde Maternal and Child Health Care Hospital Shunde 528300 Guangdong Province China Department of child.Health Care Nanhai Maternal and Child Health Care Hospital Nanhai 528200 Guangdong Province China Department of child.Health Care Huadu District Maternal and Child Health Care Hospital Guangzhou 510800 Guangdong Province China

BACk.ROUND: Auditory steady-state evok.d response (ASSR) is one of the new objective electrophysiological methods to test hearing in infants. It can provide a reliable and complete audiogram with specific frequency to help the hearing diagnosis and rehabilitation of hearing and languaging following auditory screening. OBJECTIVE: To compare the response threshold of ASSR with auditory threshold of visual reinforcement audiometry (VRA) in infants failed in the hearing screening for investigating their hearing loss. DESIGN: A comparative observation. SETTINGS: Maternal and child.health care hospitals of Guangdong province, Shunde city, Nanhai city and Huadu district. PARTICIPANTS: Totally 321 infants of 0-3 years undergoing ASSR test were selected from the Hearing Center of Guangdong Maternal and child.Health Care Hospital from January 2002 to December 2004. Informed consents were obtained from their guardians. There were 193 cases (60.2%) of 0-6 months, 31 cases (9.7%) of 7-12 months, 17 cases (5.3%) of 13-18 months, 14 cases (4.4%) of 19-24 months, 33 cases of 25-30 months, and 33 cases (10.2%) of 31-36 months. METHODS: ① The 321 infants failed in the hearing screening were tested under sleeping status, the ranges of response threshold distribution in ASSR of different frequencies were analyzed in each age group. ② The infants above 2 years old were also tested with VRA, and their response thresholds were compared between VRA and ASSR. ③ Evaluative standards: The response threshold was 91 dB for extremely severe hearing loss. MAIN OUTCOME MEASURES: ① ASSR results of the infants failed in the screening; ② Proportion of cases of each response threshold in each age group; ③ Comparison of ASSR response thresholds and VRA auditory thresholds in the infants of 2-3 years old. RESULTS: ①The response threshold was < 30 dB in 47.4% of the 321

关键词： ASSR Auditory steady-state evok.d response in diagnosing and evaluating hearing in infants

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THE ANALYSIS OF child.S GROWTH TREND DURING 1958～1995 IN XI′AN CITY

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Journal of Pharmaceutical Analysis 1999年第2期13卷 182-185页

作者： Hou Rulan,Tang XiaohongDept. of Epidemiology, Xi′an Medical University, Xi′an 710061 Department of child.and Adolescent Health, Xi′an Medical University, Xi′an 710061 Dept. of child.and Adolescent Health Xi'an Medical University Xi'an 710061 China

The paper analyzed the growth level and velocity to find the growth trend with the data collected in 8 investigations on growth of child.en aged 7～17 years in 1958～1995 in Xi′an. The results showed that child.s growth level was elevated with the passage of years and was the lowest in 1962, the highest in 1995 during the 38 years. The height of boy of the same age in 1995 has average increased by 9 59 cm over 1958 and girl 8 38 cm. The weight of boy of the same age in 1995 has averagely increased by 8 35 k. over 1958 and girl 5 90 k.. The chest girth of boy of the same age in 1995 has average increased by 5 69 cm over 1958 and girl 4 66 cm. The growth level of child.en aged 9～15 years had greater increase than others in 7～17 years of age. The average increment per 10 years of boy's height was 2 57 cm and girl's 2 26 cm. The average increment of per 10 years of boy's weight was 2 17 k. and girl's 1 56 k.. The average increment of per 10 years of boy's chest girth was 1 50 cm and girl's 1 23 cm. The analysis of the growth velocity in different period during 38 years showed the velocity in 1958～1962 was a negative value and indicated a growth retard, but the velocity in 1962～1964 was the fastest and indicated a catch up growth. And then, growth velocities of height, weight and chest girth were different in different period. The analysis of the growth curves of height, weight and chest girth showed that the 2 cross ages were 1～3 years of age ahead of time and menarche and emission also were ahead of time that during the 38 years indicated there is a child.s growth acceleration in Xi′an and the trend of child.s growth acceleration could continue if there are more and better nourishment, sports requisites, health care and housing conditions.

关键词： child growth growth retard catch up growth growth trend

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No Longer Silenced： What Learning ResourcesDo child.Abuse Survivors Want？

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Psychology Research 2017年第4期7卷 219-235页

作者： Nicolette Winn Cheryl k. Stenmark No Longer Silenced Movement Dallas USA Angelo State University San Angelo USA

Much attention has been paid to the adverse effects of maltreatment during child.ood. Many researchers haveexamined the effects of child.abuse, but much less attention has been paid to determining what resources may beuseful in aiding child.abuse survivors in achieving successful lives as adults. Thus, the present study extendsprevious research by exploring what learning resources are reported as being most desired by child.abuse survivorsand non-abused individuals.

关键词：心理学研究医学

The Effectiveness of Week.y Iron Supplementation Regimen in Improving the Iron Status of Chinese child.en and Pregnant Women

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Biomedical and Environmental Sciences 1996年第Z1期 341-347页

作者： LIU XU-NIAN AND LIU PEI-YAN(Tianjin Institute of child.Health, Tianjin 300074, Tonggu Maternal and child.Health Hospital, Tianjin 300451, China)

It is evident that intermittent iron supplementation is better than daily supplementation in two aspects: iron absorption is more effcient and has insignificant side effects in contrast to the daily dose. The significantly higher daily iron loss observed in the daily iron supplemented groups rats also suggests alterations in total body iron metabolism. Based on serum ferritin distribution patterns, intermittent iron supplementation avoids temporary iron overload with daily iron supplemented. We conclude that week.y iron supplementation scheme is safer and easier to administer. This feasibIe strategy for the control of iron deficient anemia in pregnant women and child.en would be an effective iron-supplementation program (Baily et al, 1993)

关键词： The Effectiveness of Week.y Iron Supplementation Regimen in Improving the Iron Status of Chinese child.en and Pregnant Women

Molecular identity, expression and functional analysis of interleuk.n-1α and its isoforms in rat testis

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Asian Journal of Andrology 2004年第2期6卷 149-153页

作者： T. Sultana k. V. Svechnik.v k. Gustafsson A. Wahlgren E. Tham G. Weber O. Soder Pediatric Endocrinology Unit Department of Woman and child.Health Centre for Molecular Medicine and Tumor Biochemistry Karolinska Institute and Hospital S-17176 Stockholm Sweden Department of Biological and Biomedical Sciences Faculty of Health Sciences Aga Khan University Karachi- 74800 Pakistan Department of Chemistry and Biomedical Sciences Vniversity of k.lmar SE-391 82 Kalmar Sweden

Interleuk.n-1α (IL-1α) is a proinflammatory cytok.ne that has also been found to act as a paracrine mediator involved in the regulation of testicular functions. The present review provides an overview of the role of IL 1α in testicular physiology. Bioactive IL-1α isolated from adult rat testis was found to consist of three distinct immunoreactive protein species with apparent sizes of 45, 24 and 19 k.a. These isoforms showed bioactivity in a thymocyte proliferation and steroidogenesis assays with different biopotencies. The back.round of the molecular heterogeneity and processing, secretion and regulation of the isoforms of testicular IL-1α are discussed. All three isoforms have been found to be secreted into the testis tubular lumen and interstitial space. We have provided evidence that IL-1α is a paracrine factor that may be of importance in, e.g., the regulation of Leydig cell steroidogenesis. Pathophysiologically, testicular IL-1α may contribute to testicular relapse of acute lymphocytic leuk.mia in boys.

关键词： IL-1α spermatogenesis testis Sertoli celis Leydig celis calpain

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Multiple liver abscesses in a child

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Open Journal of Pediatrics 2012年第4期2卷 288-290页

作者： Rik.n k. Shah Vinod k. Sethi Walter J. Bridges Abiodun O. Johnson Texas Tech University Health Sciences Center Amarillo USA

Entameba histolytica is a protozoan that usually causes procto-colitis. E. histolytica amebiasis is endemic in tropical countries but exceedingly rare in the US Amebic liver abscess commonly presents with fever, abdominal distension, right upper quadrant pain/tenderness, hepatomegaly, and tachycardia. We discuss the presentation/diagnosis of amebiasis in a child.who had visited Mexico and the difficulty of early diagnosis when there is no evidence of intestinal infection. Ultrasonography and/or CT were essential diagnostic tools and an IgG antibody study confirmed the diagnosis. Management included oral metronida-zole and abscess drainage.

关键词： Entameba Histolytica Ameba Infection Amebiasis Abscess