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Journal of Forensic Science and Medicine 2023年第3期9卷 264-270页

作者： k suprasanna R Anand Sheetal Harakuni S R Ravikiran Department of Radio-Diagnosis Kasturba Medical CollegeMangaloreManipal Academy of Higher EducationManipalIndia Department of Medical Education Kasturba Medical CollegeMangaloreManipal Academy of Higher EducationManipalIndia Department of Respiratory Medicine Kasturba Medical CollegeMangaloreManipal Academy of Higher EducationManipalIndia Department of Microbiology Jawaharlal Nehru Medical CollegeKLE Academy of Higher Education and ResearchBelgaviKarnatakaIndia Department of Medical Education Jawaharlal Nehru Medical CollegeKLE Academy of Higher Education and ResearchBelgaviKarnatakaIndia Department of Pediatrics Kasturba Medical CollegeMangaloreManipal Academy of Higher EducationManipalIndia

Background:Informed consent has medicolegal implications and is imperative in presentday medical *** and Objectives:The objectives of this study were to estimate the awareness regarding informed consent in interns and to determine the effectiveness of sessions in interns’orientation programs about informed *** and Methods:Pretest-Posttest study was conducted with a validated questionnaire distributed through Google Forms to interns attending the orientation program at our *** were recorded on the Likert scale(−2 to+2).Paired t-test was used to compare the mean pretest and posttest *** number of interns with a positive score(+1,+2)for every question in pretest and posttest were also ***:One hundred and seventyone out of 232 interns who attended the program submitted both pretest and posttest *** the pretest,12 interns scoredknowledge regarding various aspects of consent need to be emphasized.

关键词： Informed consent interns medicolegal orientation program

Time course of physiological,biochemical,and gene expression changes under short-term salt stress in Brassica juncea L.

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The Crop Journal 2017年第3期5卷 219-230页

作者： Manish Pandey suprasanna Penna Plant Stress Physiology and Biotechnology Section Nuclear Agriculture and Biotechnology DivisionBhabha Atomic Research CentreTrombayMumbai 400085India

Salinity-imposed limitations on plant growth are manifested through osmotic and ionic imbalances. However, because salinity-induced responses vary considerably among crop plants, monitoring of such responses at an early stage has relevance. In this study,physiological(seed germination, seed vigor index, root length, shoot length, fresh weight,dry weight) and biochemical attributes(osmoprotectants, k^+/Na^+ ratio) were analyzed for a time-course assessment of salt responses in Indian mustard(Brassica juncea L.) with an emphasis on early monitoring. The results showed strong correlations for total soluble sugars at germination phase(24 h), proline content in the seedling establishment phase(48 h) and various physiological parameters including seed vigor index(R^2= 0.901), shoot length(R^2= 0.982), and fresh weight(R^2= 0.980) at 72 h(adaptation under stress). In addition, transcriptional changes were observed under NaCl treatment for key genes belonging to the family of selective ion transporters(NHX, HkT) and abscisic acid synthesis(AAO-3). The status of mitochondrial respiration was also examined as a probe for salinity tolerance at an early stage. The results suggested that although all the analyzed parameters showed correlations(negative or positive) with salt stress magnitude, their critical response times differed, with most of the studied biochemical, physiological, or molecular markers providing valuable information only after radicle emergence, whereas mitochondrial respiration via alternative oxidase was useful for the early detection of salt responses.

关键词： Alternative oxidase Brassica Osmolytes Salinity Seed germination

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Gamma Irradiation of Embryogenic Callus Cultures and In vitro Selection for Salt Tolerance in Sugarcane (Saccharum officinarum L.)

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Agricultural Sciences in China 2008年第9期7卷 1147-1152页

作者： Vikas Y Patade P suprasanna V A Bapat Plant Cell Culture Technology Section Nuclear Agriculture & Biotechnology DivisionBhabha Atomic Research Centre

Radiation induced mutagenesis followed by in vitro selection was employed for salt tolerance in popular Indian sugarcane （Saccharum officinarum L.） cv. CoC-671. Embryogenic calli were gamma irradiated and exposed to different levels of NaCl （42.8, 85.6, 128.3, 171.1,213.9, 256.7, 299.5, or 342.2 mM）. The relative growth rate （RGR） decreased progressively with increasing salt stress and was the least with a salt stress of 256.7 mM （0.25±0.009）, almost 10 fold lesser than the control. The RGR was significantly lower in 85.6 mM and higher salt stressed calli than the control. The survival percent also decreased, with an increase in NaCl concentration. In case of 10 and 20 Gy irradiated calli, regeneration was observed up to 85.6 mM NaCl selection, medium, whereas, higher treatments （128.3 mM and beyond） exhibited browning initially. However, in the subsequent subcultures, regeneration was obtained in the case of 10 and 20 Gy irradiated calli on 128.3 and 171.1 mM NaCl selections. Higher dose of gamma irradiation （40 Gy） also showed regeneration, but only with 85.6 mM NaCI selection. The unirradiated calli regenerated the highest number of plantlets followed by 10 and 20 Gy irradiated calli on salt selection. A total of 147 plantlets were selected from different salt levels. The salt selected plants are being tested for their field performance.

关键词： sugarcane in vitro mutagenesis in vitro selection salt tolerance

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Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane(Saccharum officinarum L.)

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The Crop Journal 2015年第1期3卷 46-56页

作者： Ashok A.Nikam Rachayya M. Devarumath Akash Ahuja Harinath Babu Mahadeo G.Shitole Penna suprasanna Tissue Culture Section Vasantdada Sugar InstituteManjari (Bk) Pune 412307 India Molecular Biology and Genetic Engineering Division Vasantdada Sugar InstituteManjari (Bk) Pune 412307 India Department of Botany Pune University Plant Stress Physiology & Biotechnology Section Nuclear Agriculture & Biotechnology Division Bhabha Atomic Research Centre

Gamma ray-induced in vitro mutagenesis and selection for salt(NaC l) tolerance were investigated in sugarcane(Saccharum officinarum L.). Embryogenic callus cultures were irradiated(10 to 80 Gy) and subjected to in vitro selection by exposure of irradiated callus to NaC l(0, 50, 100,150, 200, and 250 mmol L-1). Increasing NaC l concentrations resulted in growth reduction and increased membrane damage. Salt-selected callus lines were characterized by the accumulation of proline, glycine betaine, and Na+and k+concentration. Higher accumulation of proline and glycine betaine was observed in NaC l stressed callus irradiated at 20 Gy. Na+concentration increased and k+concentration decreased with increasing salt level. Irradiated callus showed50–60% regeneration under NaC l stress, and in vitro-regenerated plants were acclimatized in the greenhouse, with 80–85% survival. A total of 138 irradiated and salt-selected selections were grown to maturity and their agronomic performance was evaluated under normal and saline conditions. Of these, 18 mutant clones were characterized for different agro-morphological characters and some of the mutant clones exhibited improved sugar yield with increased Brix%,number of millable canes, and yield. The result suggest that radiation-induced mutagenesis offers an effective way to enhance genetic variation in sugarcane.

关键词： Gamma-rays Mutagenesis Embryogenic callus Saccharum officinarum L Salt tolerance

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玉米颖片愈伤组织的幼株再生

园艺与种苗 1987年第4期 26-27页

作者： P.suprasanna 赵继海

除了植物的遗传操作外,植物细胞和组织培养正日益广泛地用作生化过程和分子过程研究的重要工具。在玉米中,早期的研究是关于甜质胚乳和非甜质胚乳的培养。此后的报道有关于从二倍体材料来源的愈伤组织诱导和保持,以及成功地从盾片培养... 详细信息

除了植物的遗传操作外,植物细胞和组织培养正日益广泛地用作生化过程和分子过程研究的重要工具。在玉米中,早期的研究是关于甜质胚乳和非甜质胚乳的培养。此后的报道有关于从二倍体材料来源的愈伤组织诱导和保持,以及成功地从盾片培养物和已萌发但未成熟胚的中胚轴切片再生出幼株。

关键词：愈伤组织盾片甜玉米创伤组织甜质型玉米幼株颖片玉米品种