检索结果-南通市图书馆

宅基地禀赋与乡城家庭化迁移——基于分期动态家庭迁移决策机制分析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

华中农业大学学报（社会科学版） 2024年第3期 213-222页

作者：邱骏吕萍中国人民大学公共管理学院北京100872

家庭化迁移已成为中国乡城迁移的主要模式,具有分期性和动态性的特征。以往的研究主要集中于土地对个体迁移决策的影响,对家庭化迁移的关注较少,也很少有研究考察土地在家庭化迁移不同阶段的影响。基于新迁移经济学理论的视角,在托达罗... 详细信息

家庭化迁移已成为中国乡城迁移的主要模式,具有分期性和动态性的特征。以往的研究主要集中于土地对个体迁移决策的影响,对家庭化迁移的关注较少,也很少有研究考察土地在家庭化迁移不同阶段的影响。基于新迁移经济学理论的视角,在托达罗模型的基础上构建分期动态的家庭化迁移决策机制,将家庭化迁移分为两个阶段:家庭离散阶段和家庭聚合阶段,并利用2017年流动人口动态监测调查数据进行实证分析。研究结果表明:(1)拥有宅基地对家庭化迁移有显著的负向影响;(2)对于拥有宅基地的家庭,宅基地面积对家庭化迁移的影响为显著的“正U型”;(3)相比于家庭离散阶段,宅基地禀赋在家庭聚合阶段的影响更大。据此建议进一步保障农民土地产权的稳定性、探索构建农村宅基地流转制度、制定满足迁移家庭基本生活需求的公共服务和户籍制度。

关键词：家庭化迁移土地禀赋土地流转乡城迁移

序列相似性家族107成员A抑制前列腺癌进展的实验研究

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中华实验外科杂志 2024年第9期41卷 2045-2050页

作者：关迪占云路全勇俊刘丹谷翊群刘跃新平浩首都医科大学附属北京同仁医院泌尿外科北京100176 北京市顺义区医院泌尿外科北京101300 国家卫健委男性生殖健康重点实验室国家卫健委科学技术研究所北京100081

目的探讨序列相似性家族107成员A(fam107A)调控缺氧诱导因子3A(H1F3A)对前列腺癌(PCa)迁移、侵袭、凋亡的影响.方法通过生物信息学技术筛选目的基因并进行分析.体外培养人PCa细胞PC-3,采用反转录病毒稳定转染,采用蛋白质印迹法(Wester... 详细信息

目的探讨序列相似性家族107成员A(fam107A)调控缺氧诱导因子3A(H1F3A)对前列腺癌(PCa)迁移、侵袭、凋亡的影响.方法通过生物信息学技术筛选目的基因并进行分析.体外培养人PCa细胞PC-3,采用反转录病毒稳定转染,采用蛋白质印迹法(Western blot)技术验证HIF3A及DNA甲基转移酶1(DNMT1)的表达量;流式细胞仪检测细胞周期及凋亡;焦磷酸测序检测启动子甲基化程度;细胞功能试验验证对PCa迁移和侵袭的影响.两组间差异表达采用独立样本t检验方法比较.结果生信分析提示fam107A基因在PCa组织中表达低于正常前列腺组织(Pfam107A表达量越低(Pfam107A会抑制 PCa 细胞的迁移(C4-2:0.337±8.930 比 29.334±2.651,t=-7.260,Pfam107A-ENZ组细胞G1期比例高于对照组(80.410±0.786比35.583±0.714,t=73.130,Pfam107A组的DNMT1表达量低于对照组(0.277±0.032 比 0.900±0.026,t=25.93,Pfam107A-5-aza 组的 HIF3A 的表达量明显恢复(0.550±0.020比0.427±0.067,t=3.073,Pfam107A时E-钙黏蛋白(E-cadherin)表达量高于对照组(0.773±0.095 比 0.307±0.046,t=7.683,Pfam107A高表达可抑制HIF3A启动子甲基化促进其表达,进而抑制PCa的迁移、侵袭,是PCa进展的生物标志物.

关键词：前列腺癌序列相似性家族107成员A 缺氧诱导因子3A DNA甲基转移酶1 细胞功能

Cost-effectiveness analysis of chemotherapy for advancedgastric cancer in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第17期14卷 2715-2722页

作者： Xin-Zu Chen Kun Jiang Jian-Kun Hu Bo Zhang Hong-Feng Gou Kun Yang Zhi-Xin Chen Jia-ping Chen Department of Gastrointestinal Surgery and Multidisciplinary Treatment Team of Gastrointestinal TumorsWest China Hospital Sichuan University Chengdu 610041Sichuan Province China Department of Integrated Western and TraditionalChinese Medicine West China Hospital Sichuan UniversityChengdu 610041 Sichuan Province China Department of Oncology West China HospitalSichuan University Chengdu 610041 Sichuan Province China

AIM： To assess the economics of various chemotherapeutic regimens for advanced gastric cancer （AGC）, and to select the best cost-effective regimen for the common Chinese patients. METHODS： Data source used in this study was the Chinese Biomedical Disk Database. Patients were diagnosed as AGC and any regimen was eligible. Outcome measures included median survival time （MST） and percentage of complete and partial response （CR＋PR）. Economic statistics was per capita direct medical cost （DMC） of a single cycle. TreeAge Pro Healthcare 2007 software was used to carry out costeffectiveness and incremental cost-effectiveness analysis. Sensitivity analyses were applied by altering willingness- to-pay and annual discount rate, and also re-analyzed by excluding the studies with apparent heterogeneity. RESULTS： Seven retrospective economics studies on 760 patients were included. S-fluorouracil-based regimens were universal, and also some new agents were involved, such as docetaxel, paclitaxel, andoxaliplatin. By processing analysis, we could recommend etoposide, leucovorin and 5-fluorouracil （ELF） regimen as preference, with a DMC/MST ratio of 2543 RBM/11.7 mo and a DMC/CR＋PR ratio of 2543 RMB/53.3%. Uraciltegafur, etoposide and cisplatin （FEP） or 5-fluorouracil, adrimycin/epirubin and mitomycin （fam） regimens could be regarded as optional first-line chemotherapy for AGC in common Chinese patients. With no regard for willingness-to-pay, the docetaxel, cisplatin and 5-fluorouracil （DCF） regimen could be chosen as either a first- or a second-line chemotherapy, with a DMC/CR＋PR ratio of 9979 RMB/56.3%. CONCLUSION： 5-fluorouracial regimens are still considered the mainstream for AGC, while new agents such as taxanes are optional. More randomized clinical trials are required before any mandatory recommendation of certain regimens for patients with AGC in China is made.

关键词： Advanced gastric cancer Chemotherapy 5-fluorouracil Taxanes Cost-effectiveness

人WASH468和WASH465蛋白质特性辨析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

医学分子生物学杂志 2024年第2期21卷 93-99页

作者：郑皑雪张鲁平汤拓李萍洪鲜邓志会王涛齐齐哈尔医学院医药科学研究院蛋白质结构与功能研究室黑龙江省齐齐哈尔市161000 齐齐哈尔医学院医学技术学院黑龙江省齐齐哈尔市161000

目的探讨两种较为公认但序列不同的Wiskott-Aldrich综合征蛋白和富含脯氨酸同源物(Wiskott-Aldrich syndrome protein and SCAR homolog,WASH)的差异。方法使用免疫荧光、免疫共沉淀和激光微辐射等实验分析两种WASH在定位模式、与fam21... 详细信息

目的探讨两种较为公认但序列不同的Wiskott-Aldrich综合征蛋白和富含脯氨酸同源物(Wiskott-Aldrich syndrome protein and SCAR homolog,WASH)的差异。方法使用免疫荧光、免疫共沉淀和激光微辐射等实验分析两种WASH在定位模式、与fam21或Ku蛋白的相互作用、向DNA损伤位点募集速率和蛋白质稳定性方面的差异;比较多种生物中的WASH蛋白质序列和检测多种在生物和医学研究中常用的细胞中的WASH编码序列,分析两种人WASH序列的普遍性和保守性。结果两种WASH展现出类似的内体(endosome)定位模式。WASH468表现出与fam21更强的相互作用,并且WASH468展现出更强的稳定性。WASH465表现出与Ku蛋白更强的相互作用,并且WASH465展现出向DNA损伤位点更强的募集。多种生物中的WASH序列与人WASH468序列的一致性明显高于WASH465,并且多种在生物和医学研究中常用的细胞中的WASH氨基酸序列均与WASH468一致。结论WASH468和WASH465的生物学特性存在差异,WASH468序列的普遍性和保守性明显高于WASH465,因此WASH468是更保守的人WASH序列。

关键词： Wiskott-Aldrich综合征蛋白和富含脯氨酸同源物蛋白序列保守性相互作用 DNA修复

碳交易政策如何影响工业碳生产率:来自中国省级数据的准自然实验

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

环境科学 2023年第5期44卷 2983-2994页

作者：房琪李绍萍东北石油大学经济管理学院大庆163318

当前,在中国奋力实现“碳达峰”“碳中和”目标的背景下,不仅给碳排放量相对较高的工业企业带来较大环境治理压力,同时也给其在经济增长方面带来较大冲击.对其随着碳交易政策的提出,是否能够产生“减碳促经”效果展开深入研究.基于2005~... 详细信息

当前,在中国奋力实现“碳达峰”“碳中和”目标的背景下,不仅给碳排放量相对较高的工业企业带来较大环境治理压力,同时也给其在经济增长方面带来较大冲击.对其随着碳交易政策的提出,是否能够产生“减碳促经”效果展开深入研究.基于2005~2019年30个省市数据,通过双重差分等方法和多重中介效应模型对碳交易政策与工业碳生产率的影响效果和作用路径展开深入分析.结果表明,碳交易政策能够明显提升工业碳生产率水平;同时该种作用效果会随着试点区域的不同存在明显差异;该政策能够产生显著的环境规制、结构优化、技术和能源结构优化效应.建议通过加速完善环境规制层面法律制度、加快构建环保产业发展新体系、加强绿色技术创新研发力度和加速优化能源结构方面促进中国工业经济向低碳化发展转型.

关键词：碳交易政策工业碳生产率双重差分模型多重中介效应模型碳达峰碳中和

Clinical and genetic features of Kenny-Caffey syndrome type 2 with multiple electrolyte disturbances:A case report

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2023年第10期11卷 2290-2300页

作者： Ning Yuan Lin Lu Xiao-ping Xing Ou Wang Yue Jiang Ji Wu Ming-Hai He Xiao-Juan Wang Le-Wei Cao Department of Endocrinology Nanchong Central HospitalThe Second Clinical CollegeNorth Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina Department of Endocrinology Key Laboratory of National health commissionPeking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical ScienceBeijing 100730China Department of Urology Nanchong Central HospitalThe Second Clinical CollegeNorth Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina

BACKGROUND Hypoparathyroidism,which can be sporadic or a component of an inherited syndrome,is the most common cause of *** hypocalcemia is accompanied by other electrolyte disturbances,such as hypokalemia and hypomagnesemia,then the cause,such as renal tubular disease,should be carefully *** SUMMARY An 18-year-old female visited our clinic because of short stature and facial deformities,including typical phenotypes,such as low ear position,depression of the nasal bridge,small hands and feet,and loss of *** lab results suggested normal parathyroid hormone but *** addition,multiple electrolyte disturbances were found,including hypokalemia,hypocalcemia and *** physical signs showed a short fourth metatarsal bone of both *** X-ray images showed cortical thickening of long bones and narrowing of the medulla of the *** computed tomography indicated calcification in the bilateral basal ***,the genetic investigation showed a de novo heterogenous mutation of“fam111A”(c.G1706A:p.R569H).Through a review of previously reported cases,the mutation was found to be the most common mutation site in Kenny-Caffey syndrome type 2(KCS2)cases reported thus far(16/23,69.6%).The mutation was slightly more prevalent in females than in males(11/16,68.8%).Except for hypocalcemia,other clinical manifestations are *** As a rare autosomal dominant genetic disease of hypoparathyroidism,the clinical manifestations of KCS2 are atypical and *** girl presented with short stature,facial deformities and skeletal *** laboratory results revealed hypocalcemia as the main electrolyte *** though her family members showed normal phenotypes,gene detection was performed to find the mutation of the fam111A gene and confirmed the diagnosis of KCS2.

关键词： Hypocalcemia Hypomagnesemia Hypoparathyroidism Kenny-Caffey syndrome type 2 fam111A gene Case report

NS1-binding protein radiosensitizes esophageal squamous cell carcinoma by transcriptionally suppressing c-Myc

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Communications 2018年第1期38卷 352-365页

作者： Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang ping Wang Dong Qian Department of Radiotherapy Tianjin Medical University Cancer Institute and HospitalTianjin 300060P.R.China National Clinical Research Center for Cancer Tianjin Medical University Cancer Institute and HospitalTianjin 300060P.R.China Key Laboratory of Cancer Prevention and Therapy TianjinTianjin’s Clinical Research Center for CancerHuanhu West StreetTianjin 300060P.R.China State Key Laboratory of Oncology in South China Guangzhou 510060GuangdongP.R.China Sun Yat-sen University Cancer Center Guangzhou 510060GuangdongP.R.China

Background:Cisplatin-based chemotherapy with concurrent radiotherapy is a standard treatment for advanced esophageal squamous cell carcinoma(ESCC).NS1-binding protein(NS1-BP),a member of the BTB-kelch protein fam-ily,has been shown to inhibit the proliferation of Hela cells by suppressing *** the present study,we examined the potential function role of NS1-BP expression in ESCC,and particularly,the sensitivity of ESCC to ***:NS1-BP expression was examined using immunohistochemistry in two cohorts(n=98 for the training cohort;n=46 for independent validation cohort)of ESCC patients receiving cisplatin-based chemotherapy and con-current *** esophageal mucosal tissue blocks were used as a *** also conducted a series of in vitro and in vivo experiments to examine the potential effects of over-expressing NS1-BP on ESCC cells,and particu-larly their sensitivity to ionizing ***:In the training cohort,NS1-BP downregulation was observed in 59%(85/144)of the ESCC ***1-BP downregulation was associated with chemoradiotherapeutic resistance and shorter disease-specific survival(DSS)in both the training and validation ***-expressing NS1-BP in cultured ESCC cells substantially increased the cellular response to irradiation both in vitro and in ***1-BP also significantly enhanced IR-induced apoptosis,and abrogated IR-induced G2/M cell-cycle arrest and ATM/Chk1 *** assays indicated that NS1-BP could interact with c-Myc promoter regions to inhibit its *** ESCC tissues,c-Myc expression was inversely correlated with NS1-BP levels,and was associated with a shorter ***:Our findings highlight the role and importance of NS1-BP in radiosensitivity of *** the NS1-BP/c-Myc pathway may provide a novel therapeutic strategy for ESCC.

关键词： Esophageal squamous cell carcinoma NS1-BP Prognostic biomarker Radiotherapy c-Myc

A target-recycling amplification strategy for sensitive and accurate detection of microRNA in cells combined with gold-based hybridization assay and generalized multivariate standard addition model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A target-recycling amplification strategy for sensitive and ...

一种突变型循环肿瘤DNA荧光检测方法的构建

2019中国化学会第十五届全国计算（机）化学学术会议

作者：闫晓梅 Zeng-ping Chen 湖南大学 College of Chemistry and Chemical Engineering Hunan University

Here, we developed a simple but reliable fluorescence signal-on platform with unique separation and purification for detection of miRNA-155 by an integration of target-recycling amplification and gold-assisted signal amplification. In this study, we indirectly detect mi RNA-155 by quantifying the target DNA which is reverse transcribed from miRNA-155. The idea is to take the target DNA open the probe DNA with a hairpin structure, then initiate the enzymatic cleavage of probe DNA, while the target DNA and residual DNA with fluorescent group（fam） are released. The released target DNA can continue to react with unreacted probe DNA and trigger target-recycling amplification to release many residual DNA. The released residual DNA, complementary with the capture DNA immobilized on the surface of pieces of gold, can trigger the second cycle--gold-assisted signal amplification. And then, the residual DNA can be separated from the original complex system to another new buffer system. It allow for a significantly enhanced fluorescence signal. In addition, a calibration model named generalized multivariate standard addition（GMSA） wsa employed to analyze spectral data, which can eliminate some unavoidable interference. This amplification method has been successfully applied to the quantitative analysis of mi RNA-155 in three types of cells, and the accuracy was comparable to the reference method--quantitative reverse transcriptase polymerase chain reaction（qRT-PCR）. The limit of detection and limit of quantification of the proposed for the detection of miRNA-155 were estimated to be 36.3 fM and 0.11 pM, respectively. Moreover, the recovery rates ranging from 93.3% to 116% were quite acceptable. Therefore, it may have great potential for early clinical diagnosis of miRNAs-related disease.

关键词： Fluorescence miRNA-155 target-recycling gold-recycling generalized multivariate standard addition

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

广州化工 2024年第11期52卷 53-55页

作者：包京京李雨鸣陈志平夏江浩田媛媛于妍妍李瑾徐州医科大学医学技术学院江苏徐州221004 徐州医科大学第一临床医学院江苏徐州221004 徐州医科大学护理学院江苏徐州221004 徐州医科大学药学院江苏徐州221004

首先合成二氧化硅包覆Fe_(3)O_(4)磁性纳米粒子(Fe_(3)O_(4)-SiO_(2)),然后连接目标物T790M的识别核酸链(DNA1),制备T790M的捕获探针(Fe_(3)O_(4)-SiO_(2)-DNA1)。进一步加入荧光素fam标记的DNA2探针。当T790M存在时,DNA2,Fe_(3)O_(4)-SiO_(2)-DNA1与T790M发生DNA杂交。磁性分离后,上清液中DNA2上连接的fam的荧光强度显著下降,且荧光强度与T790M浓度之间具有良好的线性相关性,线性回归方程为F=-142.56lgC+739.24(R^(2)=0.994),检测限为0.02 nmol/L。本方法为恶性肿瘤的精确诊断提供了一种有效的技术。

关键词：循环肿瘤DNA(ctDNA) DNA杂交磁性分离荧光分析恶性肿瘤