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Matrix metalloproteinase-9 in the initial injury after hepatectomy in mice

World Journal of Gastroenterology 2013年第20期19卷 3027-3042页

作者： Norifumi Ohashi Tomohide Hori Florence chen Sura Jermanus Akimasa Nakao Shinji Uemoto justin H Nguyen Gastroenterological Surgery Nagoya University Graduate School of MedicineNagoyaAichi 466-8550Japan Divisions of Hepato-PancreatoBiliary Transplant and Pediatric SurgeryDepartment of SurgeryKyoto University Graduate School of MedicineKyoto 606-8507Japan Department of Neuroscience Mayo ClinicJacksonvilleFL 32224United States Division of Transplant Surgery Department of TransplantationMayo Clinic in FloridaJacksonvilleFL 32224United States

AIM:To investigate the role of matrix metalloproteinase(MMP)-9 in the pathogenesis of postoperative liver failure(PLF) after extended hepatectomy(EH).METHODS:An insufficient volume of the remnant liver(RL) results in higher morbidity and mortality,and a murine model with 80%-hepatectomy was *** investigations were performed 6 h after *** were first divided into two groups based on the postoperative course(i.e.,the PLF caused or did not),and MMP-9 expression was measured by Western *** source of MMP-9 was then determined by immunohistological *** inhibitor of metalloproteinase(TIMP)-1 is the endogenous inhibitor of MMP-9,and MMP-9 behavior was assessed by the experiments in wild-type,MMP-9(-/-) and TIMP-1(-/-) mice by Western blotting and gelatin *** behavior of neutrophils was also assessed by immunohistological *** anti-MMP-9 monoclonal antibody and a broadspectrum MMP inhibitor were used to examine the role of ***:Symptomatic mice showed more severe PLF(histopathological assessments:2.97 ± 0.92 vs 0.11 ± 0.08,P < 0.05) and a higher expression of MMP-9(71085 ± 18274 vs 192856 ± 22263,P < 0.01).Nonnative leukocytes appeared to be the main source of MMP-9,because MMP-9 expression corresponding with CD11b positive-cell was observed in the findings of immunohistological *** the histopathological findings,the PLF was improved in MMP-9(-/-) mice(1.65% ± 0.23% vs 0.65% ± 0.19%,P < 0.01) and it was worse in TIMP-1(-/-) mice(1.65% ± 0.23% vs 1.78% ± 0.31%,P < 0.01).Moreover,neutrophil migration was disturbed in MMP-9(-/-) mice in the immunohistological *** methods of MMP-9 inhibition revealed reduced PLF,and neutrophil migration was strongly disturbed in MMP-9-blocked mice in the histopathological assessments(9.6 ± 1.9 vs 4.2 ± 1.2,P < 0.05,and 9.9 ± 1.5 vs 5.7 ± 1.1,P < 0.05).CONCLUSION:MMP-9 is important for the process of *** initial injury is associated with MMP-9 derived from neutrophil

关键词： Matrix metalloproteinase Shear stress Sinusoidal injury Hepatectomy Portal hypertension

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A 24 hour naproxen dose on gastrointestinal distress and performance during cycling in the heat

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Sports Medicine and Health Science 2020年第1期2卷 19-24页

作者： Dawn M.Emerson J.Mark Davis Stephen CL.chen Toni M.Torres-McGehee Craig E.Pfeifer Charles C.Emerson Joseph D.Bivona justin V.Stone Department of Health Sportand Exercise SciencesUniversity of KansasLawrenceKansasUSA Department of Exercise Science University of South CarolinaColumbiaSCUSA Division of Exercise and Sport Science Bob Jones UniversityGreenvilleSouth CarolinaUSA Applied Sport and Exercise Sciences The ParkUniversity of GloucestershireCheltenham GL502RHUnited Kingdom Department of Athletics University of Missouri-Kansas CityKansas CityUSA

Using a double-blind,randomized and counterbalanced,cross-over design,we assessed naproxen's effects on gastrointestinal(GI)distress and performance in eleven volunteers(6 male,5 female).Participants completed 4 trials:1)placebo and ambient);2)placebo and heat;3)naproxen and ambient;and 4)naproxen and *** variables were one placebo or 220 mg naproxen pill every 8 h(h)for 24 h and ambient(22.7±1.8℃)or thermal environment(35.7±1.3℃).Participants cycled 80 min at a steady heart rate then 10 min for maximum *** exertion was measured throughout *** distress was assessed pre-,during,post-,3 h post-,and 24 h post-cycling using a GI index for upper,lower,and systemic *** statistically significant differences occurred between conditions at any time for GI symptoms or perceived exertion,distance,or heart rate during maximum effort.A 24 h naproxen dose did not significantly affect performance or cause more frequent or serious GI distress when participants were euhydrated and cycling at moderate intensity in a thermal environment.

关键词： NSAID Gastrointestinal bleeding Heart rate Perceived exertion

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Adenoviral transduction of PTEN induces apoptosis of cultured hepatic steUate cells

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Chinese Medical Journal 2009年第23期122卷 2907-2911页

作者： HAO Li-sen ZHANG Xiao-lan AN Jun-yan YAO Dong-mei justin Karlin FANG Shu-ming JIANG Hui-qing BAI Wen-yuan chen Shuang Department of Gastroenterology Second Hospital of HebeiMedical University Hebei Key Laboratory of GastroenterologyHebei Institute of Gastroenterology Shijiazhuang Hebei 050000China Department of Pediatric UCLA Medical School Cedars-SinaiMedical Center Los Angeles CA 90048 USA

Hepatic fibrosis is the liver＇s wound healing response to virtually all forms of chronic liver injury： toxic insult, viral infection, immunological conditions and metabolic diseases. Uncontrolled liver fibrosis eventually results in cirrhosis and associated complications, such as cancer and liver failure. Given the enormous public health implications, recent improvements in the treatment of chronic liver disease have accelerated interest in uncovering the mechanisms underlying hepatic fibrosis and its resolution. Currently, it is believed that activation of quiescent hepatic stellate cells （HSC） into proliferative, contractile, and fibrogenic cells in response to liver injury appears to be the dominant driving force in fibrosis.

关键词： PTEN protein hepatic stellate cells fibrosis apoptosis

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Growth and fabrication of semi-polar InGaN/GaN multi-quantum well light-emitting diodes on microstructured Si(001) substrates

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Chinese Physics B 2015年第11期24卷 530-533页

作者：陈龙裴嘉鼎史达特李成张建明俞文杰狄增峰王曦 State Key Laboratory of Functional Materials for Informatics Institute of Microsystem and Information TechnologyChinese Academy of Sciences Shanghai Sim?ra Incorporated

Semi-polar （1 - 101 ） InGaN/GaN light-emitting diodes were prepared on standard electronic-grade Si （100） substrates. Micro-stripes of GaN and InGaN/GaN quantum wells on semi-polar facets were grown on intersecting { 111 } planes of microscale V-grooved Si in metal-organic vapor phase epitaxy, covering over 50% of the wafer surface area. In-situ optical reflectivity and curvature measurements demonstrate that the effect of the thermal expansion coefficient mismatch was greatly reduced. A cross-sectional analysis reveals low threading dislocation density on the top of most surfaces. On such prepared （1-101） GaN, an InGaN/GaN LED was fabricated. Electroluminescence over 5 mA to 60 mA is found with a much lower blue-shift than that on the c-plane device. Such structures therefore could allow higher efficiency light emitters with a weak quantum confined Stark effect throughout the visible spectrum.

关键词： metalorganic chemical vapor deposition semipolar light emitting diodes

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CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin chen Danping chen Fuqing chen Fusan chen Gang chen Guoming chen Hua-Xing chen Huirun chen Jinhui chen Ji-Yuan chen Kai chen Mali chen Mingjun chen Mingshui chen Ning chen Shanhong chen Shanzhen chen Shao-Long chen Shaomin chen Shiqiang chen Tianlu chen Wei chen Xiang chen Xiaoyu chen Xin chen Xun chen Xurong chen Ye chen Ying chen Yukai chen Zelin chen Zilin chen Gang chen Boping chen Chunhui chen Hok Chuen cheng Huajie cheng Shan cheng Tongguang cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi Ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding Haiyi Dong Jianin AGH University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui University HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu University Institute of ScienceVaranasi Bandirma Onyedi Eylul University Bandirma Beihang University Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal University Beijing Brown University ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad University Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo University Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central China Normal University WuhanHubei Central South University ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang University HangzhouZhejiang China Nuclear Instrument CO. LTDBeijing China University of Geosciences Beijing China University of Geosciences WuhanHubei China University of Mining and Technology XuzhouJiangsu China West Normal University NanchongSichuan Chongqing University Department of PhysicsChongqing Chulalongkorn University Bangkok City University of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell University IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu University DalianLiaoning Dalian University of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East China University Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

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全局检验与事后检验之间的联系:ANOVA方差分析中F检验结果的一项调查（英文）

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上海精神医学 2018年第1期30卷 60-64页

作者： chen T Xu M Tu J Wang H Niu X Department of Mathematics and Statistics University of Toledo Shanghai Mental Health Center Shanghai Jiao Tong University Medical College Department of Physical Medicine and Rehabilitation University of Virginia School of Medicine Department of Biostatistics and Computational Biology University of Rochester College of Informatics Huazhong Agriculture University

多组比较在许多生物医学和心理社会学研究中是一种常见的统计检验。当组数为两个以上时,首先对组间的整体差异进行全局检验。如果零假设被拒绝,则接着进行下一步的事后两两组间比较,以确定差异的来源。相反,第一步停止即可声明没有组间... 详细信息

多组比较在许多生物医学和心理社会学研究中是一种常见的统计检验。当组数为两个以上时,首先对组间的整体差异进行全局检验。如果零假设被拒绝,则接着进行下一步的事后两两组间比较,以确定差异的来源。相反,第一步停止即可声明没有组间差异。一个共同认识是如果全局检测结果是显著的,那么至少存在两个组之间是有显著差异的,反之亦然。因此,当全局检测结果显著,但没有事后组间两两比较没有显著差异,人们会感到困惑发生了什么并想知道如何来解释该结果。总之,当全局检测结果不显著时,人们会想知道是否所有的事后检测也都不显著?同样,全局检测结果不显著而停止事后检测是否会导致发现组差差异的丢失?在这篇报告中,我们研究了这个令人费解的现象,并且讨论了如何解释这样的结果。

关键词：全局检测事后检验 F检验图基(Tukey)检验

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产业创新生态系统的形成与演进:“架构者”变迁及其战略行为演变

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管理世界 2021年第9期37卷 167-190,235页

作者：谭劲松宋娟陈晓红约克大学舒力克商学院中南大学商学院湖南工商大学

架构者变迁及其战略行为演变如何促进产业创新生态系统形成和演进?本文以中国轨道交通装备产业为对象,采用纵向案例方法探究这个问题。研究结果表明:在产业创新生态系统演进的不同阶段,"架构者"动态变迁,不同架构者战略行为及其作用机... 详细信息

架构者变迁及其战略行为演变如何促进产业创新生态系统形成和演进?本文以中国轨道交通装备产业为对象,采用纵向案例方法探究这个问题。研究结果表明:在产业创新生态系统演进的不同阶段,"架构者"动态变迁,不同架构者战略行为及其作用机制不同;同一架构者不同时期战略行为和作用机制也不同。新生期,政府作为"架构者",通过行政干预自上而下构建产业创新生态系统雏形。扩展期和提升期,政府角色跨层次分离:向上成为宏观制度构建者,通过不断减弱的行政管理、增强的产业政策和科技资金资助,作为外部力量推动产业创新生态系统演进;向下作为产业创新生态系统参与主体——关键用户,促进产业创新生态系统演进。成熟期核心企业成为产业创新生态系统架构者,自底向上推动产业创新生态系统演进。本研究从中观产业层次拓展了创新生态系统战略和架构演进研究,深化并丰富了大型复杂产品产业中政府角色和作用机制研究;为政府、企业培育产业创新生态系统提供启示。

关键词：产业创新生态系统形成与演进架构者变迁战略行为演变政府角色干预组合中国轨道交通装备产业

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Hypothermia predicts hepatic failure after extensive hepatectomy in mice

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World Journal of Hepatology 2013年第4期5卷 170-181页

作者： Norifumi Ohashi Tomohide Hori Shinji Uemoto Sura Jermanus Feng chen Akimasa Nakao justin H Nguyen Gastroenterological Surgery Nagoya University Graduate School of MedicineNagoyaAichi 466-8550Japan Division of Hepato-BiliaryPancreatic and Transplant Surgery Department of SurgeryKyoto University Graduate School of MedicineKyoto 606-8507Japan Department of Neuroscience Mayo ClinicJacksonvilleFL 32224United States Division of Transplant Surgery Department of TransplantationMayo ClinicJacksonvilleFL 32224United States

AIM:To investigate the effect of hypothermia on the function of the liver remnant(LR)after extended ***:We performed a 75% partial hepatectomy(PH)in male C57BL/6J *** temperature was measured with a rectal *** study mice were prospectively grouped as hypothermic(HT)or normothermic(NT)if their body temperature was < 34 ℃ vs≥ 34 ℃,*** and liver samples were obtained at 24 and 48 h after 75% *** factors during and after 75% PH were compared at each time point and the most important factor for a good outcome after 75% PH was ***:At 24 and 48 h after 75% PH,LR weight was decreased in HT mice compared with that in NT mice and the assay results in the HT mice were consistent with liver *** mice had normal liver *** intra-and post-operative factor which showed statistical significance in univariate analysis was evaluated by multivariate *** most important factor for a good outcome after 75% PH was body temperature at both 24 and 48 h after ***:Hypothermia after an extensive hepatectomy predicts impending liver failure and may be a useful clinical marker for early detection of liver failure after extended hepatectomy.

关键词： Hypothermia Predictor Hepatectomy Liver failure Mortality

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A Microarray Based Genomic Hybridization Method for Identification of New Genes in Plants：Case Analyses of Arabidopsis and Oryza

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Journal of Integrative Plant Biology 2007年第6期49卷 915-926页

作者： Chuanzhu Fan Maria D. Vibranovski Ying chen Manyuan Long Department of Ecology and Evolution The University of Chicago Chicago IL 60637 USA

To systematically estimate the gene duplication events In closely related species, we have to use comparative genomlc approaches, either through genomlc sequence comparison or comparative genomlc hybridization （CGH）. Given the scarcity of complete genomlc sequences of plant species, in the present study we adopted an array based CGH to Investigate gene duplications In the genus Arabldopsls. Fragment genomlc DNA from four species, namely Arabidopsls thallana, A. lyrata subsp, lyrata, A. lyrata subsp, petraea, and A. halleri, was hybridized to Affymetrlx （Santa Clara, CA, USA） tiling arrays that are designed from the genomlc sequences of A. thallana. Pairwlse comparisons of signal intensity were made to infer the potential duplicated candidates along each phylogenetic branch. Ninety-four potential candidates of gene duplication along the genus were Identified. Among them, the majority （69 of 94） were A. thallana lineage specific. This result indicates that the array based CGH approach may be used to Identify candidates of duplication In other plant genera containing closely related species, such as Oryza, particularly for the AA genome species. We compared the degree of gene duplication through retrotransposon between O. satlva and A. thallana and found a strikingly higher number of chimera retroposed genes In rice. The higher rate of gene duplication through retroposltlon and other mechanisms may Indicate that the grass species Is able to adapt to more diverse environments.

关键词： Arabidopsis comparative genomic hybridization microarray new genes Oryza retroposition.

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High-Throughput Chemotyping of Cannabis and Hemp Extracts Using an Ultraviolet Microplate Reader and Multivariate Classifiers

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Journal of Analysis and Testing 2018年第3期2卷 210-222页

作者： Zewei chen Peter de Boves Harrington Steven F.Baugh Clippinger Laboratories Department of Chemistry and BiochemistryCenter for Intelligent Chemical InstrumentationOhio UniversityAthensOH 45701USA Chemistry Mapping Inc.5902 McIntyre St.GoldenCO 80403USA

As the use of Cannabis products as natural medicines burgeons,it is also appearing as a food *** is important to screen Cannabis samples as ingredients by profiling their chemical compositions,which is referred to as *** sets of botanical extracts were *** first set is referred to as Cannabis contained plant materials from 15 samples of the sativa,indica,and hybrids of the two *** second set contained 20 extracts from the variety of Cannabis sativa with low tetrahydrocannabinol(THC)concentrations,i.e.,below 0.3%,and,henceforth,will be referred to as *** ultraviolet(UV)microplate reader provides a cost-effective and high-throughput method for identifying chemotypes of plant extracts by their *** microplate reader affords rapid measurements of small volumes,e.g.,50μL,which demonstrates a potential to significantly reduce the analysis time and cost for Cannabis and hemp chemotyping or chemi-cal *** samples were measured on different days to demonstrate the robustness of the *** difference resolution(PDR)maps were used to visualize the separations among the *** multivariate classifiers,fuzzy rule-building expert system(FuRES),super partial least squares-discriminant analysis(sPLS-DA),support vector machine(SVM),and two tree-based support vector machines(SVMtreeG and SVMtreeH)were *** classifiers were validated with ten bootstrapped Latin partitions(BLPs).For the Cannabis extracts,the SVMtreeG yielded the best performance and the classification accuracy was 99.1±0.4%for spectra collected in the nonlinear absorbance *** the hemp extracts,the SVM classifier performed the best with a 97.4±0.6%classification *** results demonstrate that the UV microplate reader coupled with multivariate classifiers can be used as a high-throughput and cost-effective approach for chemotyping Cannabis.

关键词： Cannabis extracts Hemp extracts Ultraviolet microplate reader Multivariate models High-throughput chemotyping Chemometrics Projected difference resolution map

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