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Transcriptome analyses of insect cells to facilitate baculovirus-insect expression

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Protein & Cell 2016年第5期7卷 373-382页

作者： Kai Yu Yang Yu xiaoyan Tang Huimin Chen junyu xiao xiao-Dong Su Biodynamic Optical Imaging Center School of Life Science Peking University Beijing 100871 China State Key Laboratory of Protein and Plant Gene Research Peking University Beijing 100871 China Peking-Tsinghua Center for Life Sciences Peking University Beijing 100871 China

The High Five cell line （BTI-TN-5B1-4） isolated from the cabbage looper, Trichoplusia ni is an insect cell line widely used for baculovirus-mediated recombinant protein expression. Despite its widespread application in industry and academic laboratories, the genomic background of this cell line remains unclear. Here we sequenced the transcriptome of High Five cells and assembled 25,234 transcripts. Codon usage analysis showed that High Five cells have a robust codon usage capacity and therefore suit for expressing proteins of both eukaryotic- and prokaryotic-origin. Genes involved in glycosylation were profiled in our study, providing guidance for engineering glycosylated proteins in the insect cells. We also predicted signal peptides for transcripts with high expression abundance in both High Five and Sf21 cell lines, and these results have important implications for optimizing the expression level of some secretory and membrane proteins.

关键词： High Five cell line baculovirus-insect cellsystem codon usage glycosylation signal peptide

A non-ACE2-blocking neutralizing antibody against Omicron-included SARS-CoV-2 variants

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Signal Transduction and Targeted Therapy 2022年第2期7卷 372-374页

作者： xiaomin Duan Rui Shi Pulan Liu Qingrui Huang Fengze Wang Xinyu Chen Hui Feng Weijin Huang junyu xiao Jinghua Yan CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking-Tsinghua Center for Life SciencesBeijing Advanced Innovation Center for GenomicsPeking UniversityBeijing 100871China Shanghai Junshi Biosciences Co.Ltd Shanghai 200126China Division of HIV/AIDS and Sextransmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China

Dear Editor,The emergence of severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)variants threatens efforts to contain the coronavirus disease 2019(COVID-19)***(B.1.1.529),the fifth novel SARS-CoV-2 variant of concern(VOC),harbors 15 mutations in the receptor-binding domain(RBD)of the spike(S)protein.1 These mutations include almost all the sites of existing VOCs(Alpha/B.1.1.7,Beta/B.1.351,Gamma/P.1,and Delta/B.1.617.2).2–4 Importantly,the key mutations directly interact with the ACE2 receptor and constitute the main target of neutralizing antibodies(NAbs),which is believed to alter the sensitivity to a large number of NAbs.

关键词： ACE2 acute neutral

Structure of unliganded membrane-proxima domains FN4-FN5-FN6 of DCC

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Protein & Cell 2017年第9期8卷 701-705页

作者： Lorenzo I. Finci Jie Zhang Xiaqin Sun Robert G. Smock Rob Meijers Yan Zhang junyu xiao Jia-huai Wang State Key Laboratory of Biomembrane and Membrane Biotechnology College of Life Sciences Peking University Dana-Farber Cancer Institute Harvard Medical School Boston MA 02215 USA European Molecular Biology Laboratory (EMBL) Hamburg Out-station Hamburg Germany PKU-IDG/McGovern Institute for Brain Research Peking University Beijing 100871 China State Key Laboratory of Protein and Plant Gene Research School of Life Sciences and Peking-Tsinghua Center for Life Sciences Peking University Beijing 100871 China Beijing Advanced Innovation Center for Struc tural Biology Tsinghua-Peking Center for Life Sciences School of Life Sciences and School of Medicine Tsinghua University Beijing 100084 China The Francis Crick Institute London NW12BE UK

Dear Editor, Deleted in colorectal cancer （DCC） is a single pass transmembrane glycoprotein that was originally identified in humans as a candidate tumor suppressor （Fearon et al., 1990）. DCC belongs to the immunoglobulin superfamily, and the extracellular fragment is composed of four immunoglobu- lin-like （Ig-like） domains followed by six fibronectin type III （FN） domains. DCC plays a pivotal role in axon guidance by mediating a combination of attractive and repulsive effects through interactions with the diffusible guidance cue,

关键词： AXON GUIDANCE RECEPTOR DCC NETRIN-1 ASSOCIATION MECHANISM INSIGHT DRAXIN GENE

Production of a human single-chain variable fragment antibody against esophageal carcinoma

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World Journal of Gastroenterology 2004年第18期10卷 2619-2623页

作者： Ming-YanXu xiao-HuXu Geng-ZhenChen xiao-LingDeng JonathanLi xiao-junyu Mei-ZhenChen DepartmentofForensicMedicine MedicalCollegeShantouUniversityShantou515041GuangdongProvinceChina SecondAffiliatedHospitalofMedicalCollege ShantouUniversityShantou515041GuangdongProvinceChina DepartmentofPreventiveMedicine MedicalCollegeShantouUniversityShantou515041GuangdongProvinceChina UniversityofCalifornia SanFranciscoUSA

AIM: To construct a phage display library of human singlechain variable fragment (scFv) antibodies associated with esophageal cancer and to preliminarily screen a scFvantibody against esophageal ***: Total RNA extracted from metastatic lymph nodes of esophageal cancer patients was used to construct a scFv gene library. Rescued by M13K07 helper phage, the scFv phage display library was constructed, esophageal cancer cell line Eca 109 and normal human esophageal epithelial cell line (NHEEC) were used for panning and subtractive panning of the scFv phage display library to obtain positive phage clones. Soluble scFv was expressed in ***2151 which was transfected with the positive phage clone, then purified by affinity *** molecular mass of soluble scFv was estimated by Western blotting, its bioactivity was detected by cell ELISA assay. Sequence of scFv was determined using the method of dideoxynucleotide ***: The size of scFv gene library was approximately 9×10^6 clones. After four rounds of panning with Eca109 and three rounds of subtractive panning with NHEEC cells,25 positive phage clones were obtained. Soluble scFv was found to have a molecular mass of 31 ku and was able to bind to Ecal09 cells, but not to HeLa and NHEEC *** heavy (VH) gene from one of the positive clones was shown to be derived from the γ chain subgroup IV of immunoglobulin, and variable light (VL) gene from the κ chain subgroup I of ***: A human scFv phage display library can be constructed from the metastatic lymph nodes of esophageal cancer patients. A whole human scFv against esophageal cancer shows some bioactivity.

关键词：单链可变片段抗体食道癌肿瘤上皮细胞

Characteristics of Elderly-Onset Rheumatoid Arthritis Patients With COVID-19

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Infectious Microbes & Diseases 2024年第1期6卷 36-42页

作者： Lanlan xiao Xinlei Ma Jinzhi Wu junyu Liang Weiqian Chen Jin Lin Department of Rheumatology the First Affiliated HospitalZhejiang University School of MedicineHangzhouChina

This study aimed to investigate the clinical characteristics of elderly-onset rheumatoid arthritis(EORA)patients with *** ret-rospectively enrolled EORA patients diagnosed with COVID-19 from December 2022 through February 2023 and followed them *** characteristics of the patients and clinical data on COVID-19 were collected,and EORA-related data at the onset of rheu-matoid arthritis and during COVID-19 episodes were evaluated.A total of 102 EORA outpatients were enrolled and stratified into the COVID-19 group(n=60)and the non–COVID-19 group(n=42).In the COVID-19 group,56(93.3%)had symptoms,8(13.3%)had viral pneumonia,and 4(6.7%)developed severe *** logistic regression analysis showed that lack of COVID-19 vac-cination(P=0.016 and P=0.030,respectively)and having interstitial lung disease(P=0.013 and P=0.001,respectively)were inde-pendent risk factors for viral pneumonia and severe *** with the general population,EORA patients did not show significantly higher susceptibility to COVID-19 or a higher risk of severe ***,EORA patients with interstitial lung disease require special consideration during COVID-19 episodes.

关键词： COVID-19 elderly-onset rheumatoid arthritis COVID-19 vaccine interstitial lung disease

Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry

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Science China(Life Sciences) 2022年第4期65卷 701-717页

作者： Shiyou Zhu Ying Liu Zhuo Zhou Zhiying Zhang Xia xiao Zhiheng Liu Ang Chen xiaojing Dong Feng Tian Shihua Chen Yiyuan Xu Chunhui Wang Qiheng Li Xuran Niu Qian Pan Shuo Du junyu xiao Jianwei Wang Wensheng Wei Biomedical Pioneering Innovation Center Beijing Advanced Innovation Center for GenomicsPeking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking UniversityBeijing100871China State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing Advanced Innovation Center for GenomicsPeking UniversityBeijing 100871China NHC Key Laboratory of Systems Biology of Pathogens and Christophe Merieux Laboratory Institute of Pathogen BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeKey Laboratory of Respiratory Disease PathogenomicsChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Academy for Advanced Interdisciplinary Studies Peking UniversityBeijing 100871China

The outbreak of coronavirus disease 2019(COVID-19) caused by SARS-CoV-2 has created a global health crisis. SARS-CoV-2 infects varieties of tissues where the known receptor ACE2 is low or almost absent, suggesting the existence of alternative viral entry pathways. Here, we performed a genome-wide barcoded-CRISPRa screen to identify novel host factors that enable SARS-CoV-2 infection. Beyond known host proteins, i.e., ACE2, TMPRSS2, and NRP1, we identified multiple host components,among which LDLRAD3, TMEM30A, and CLEC4G were confirmed as functional receptors for SARS-CoV-2. All these membrane proteins bind directly to spike’s N-terminal domain(NTD). Their essential and physiological roles have been confirmed in either neuron or liver cells. In particular, LDLRAD3 and CLEC4G mediate SARS-CoV-2 entry and infection in an ACE2-independent fashion. The identification of the novel receptors and entry mechanisms could advance our understanding of the multiorgan tropism of SARS-CoV-2, and may shed light on the development of COVID-19 countermeasures.

关键词： CRISPRa screen SARS-CoV-2 novel receptors

Tunable terahertz absorber based on transparent and flexible metamaterial

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Chinese Optics Letters 2020年第9期18卷 69-73页

作者： junyu xiao Ruiwen xiao Rongxuan Zhang Zhixiong Shen Wei Hu Lei Wang Yanqing Lu College of Electronic and Optical Engineering&College of Microelectronics Nanjing University of Posts and TelecommunicationsNanjing 210023China National Laboratory of Solid State Microstructures Key Laboratory of Intelligent Optical Sensing and Manipulationand College of Engineering and Applied SciencesNanjing UniversityNanjing 210093China

We demonstrate a tunable terahertz(THz) absorber based on an indium tin oxide(ITO) metamaterial. The upper ITO cross-shaped metasurface with different arm lengths is fabricated by direct femtosecond laser *** thickness of the middle dielectric layer is only 60 μm, which makes the absorber very transparent and flexible. The experimental results show that the THz resonant peaks have a high performance near 1 THz. By setting spacers of different thicknesses between the middle layer and the ITO mirror, a new type of tunable THz absorber is proposed. Its absorption peak frequency can be continuously adjusted from 0.92 to 1.04 THz between TE and TM polarization. This transparent THz metamaterial absorber is expected to be widely used in THz imaging, sensing, and biological detection.

关键词： tunable terahertz absorber transparent metamaterial flexible metamaterial

Enhancing layered perovskite ferrites with ultra-high-density nanoparticles via cobalt doping for ceramic fuel cell anode

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Journal of Energy Chemistry 2024年第9期96卷 39-48页

作者： Shuo Zhai Rubao Zhao Hailong Liao Ling Fu Senran Hao junyu Cai Yifan Wu Jian Wang Yunhong Jiang Jie xiao Tao Liu Heping Xie State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering Shenzhen University&Sichuan UniversityShenzhen 518060GuangdongChina National and Local Joint Engineering Research Center for Lithium-ion Batteries and Materials Preparation Technology Key Laboratory of Advanced Battery Materials of Yunnan ProvinceFaculty of Metallurgical and Energy EngineeringKunming University of Science and TechnologyKunming 650093YunnanChina School of Energy and Environment City University of Hong KongKowloon 999077Hong KongChina

Nanoparticles anchored on the perovskite surface have gained considerable attention for their wide-ranging applications in heterogeneous catalysis and energy conversion due to their robust and integrated structural ***,we employ controlled Co doping to effectively enhance the nanoparticle exsolution process in layered perovskite ferrites *** alloy nanoparticles with ultra-high-density are exsolved on the(PrBa)_(0.95)(Fe_(0.8)Co_(0.1)Nb_(0.1))2O_(5+δ)(PBFCN_(0.1))surface under reducing atmosphere,providing significant amounts of reaction sites and good durability for hydrocarbon *** a reducing atmosphere,cobalt facilitates the reduction of iron cations within PBFCN_(0.1),leading to the formation of CoFe alloy *** formation is accompanied by a cation exchange process,wherein,with the increase in temperature,partial cobalt ions are substituted by ***,Co doping significantly enhance the electrical conductivity due to the stronger covalency of the Cosingle bondO bond compared with Fesingle bondO bond.A single cell with the configuration of PBFCN_(0.1)-Sm_(0.2)Ce_(0.8)O_(1.9)(SDC)|SDC|Ba_(0.5)Sr_(0.5)Co_(0.8)Fe_(0.2)O_(3−δ)(BSCF)-SDC achieves an extremely low polarization resistance of 0.0163Ωcm^(2)and a high peak power density of 740 mW cm^(−2)at 800℃.The cell also shows stable operation for 120 h in H_(2)with a constant current density of 285 mA cm^(−2).Furthermore,employing wet C_(2)H_(6)as fuel,the cell demonstrates remarkable performance,achieving peak power densities of 455 mW cm^(−2)at 800℃and 320 mW cm^(−2)at 750℃,marking improvements of 36%and 70%over the cell with(PrBa)_(0.95)(Fe_(0.9)Nb_(0.1))_(2)O_(5+δ)(PBFN)-SDC at these respective *** discovery emphasizes how temperature influences alloy nanoparticles exsolution within doped layered perovskite ferrites materials,paving the way for the development of high-performance ceramic fuel cell anodes.

关键词： Solid oxide fuel cell Anode Ethane fuel Nanoparticle Exsolution Layered perovskite Ferrites

Gut microbial metabolite targets HDAC3-FOXK1-interferon axis in fibroblast-like synoviocytes to ameliorate rheumatoid arthritis

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Bone Research 2024年第2期12卷 421-437页

作者： Hongzhen Chen Xuekun Fu xiaohao Wu Junyi Zhao Fang Qiu Zhenghong Wang Zhuqian Wang Xinxin Chen Duoli Xie Jie Huang junyu Fan Xu Yang Yi Song Jie Li Dongyi He Guozhi xiao Aiping Lu Chao Liang Department of Systems Biology School of Life SciencesSouthern University of Science and TechnologyGuangdong Provincial Key Laboratory of Cell Microenvironment and Disease ResearchShenzhen Key Laboratory of Cell MicroenvironmentShenzhen 518055China Institute of Integrated Bioinfomedicine and Translational Science(IBTS) School of Chinese MedicineHong Kong Baptist UniversityHong Kong SAR 999077China Department of Biochemistry School of MedicineSouthern University of Science and TechnologyGuangdong Provincial Key Laboratory of Cell Microenvironment and Disease ResearchShenzhen Key Laboratory of Cell MicroenvironmentShenzhen 518055China Division of Immunology and Rheumatology Stanford UniversityStanfordCA 94305USA VA Palo Alto Health Care System Palo AltoCA 94304USA Institute of Plant and Food Science Department of BiologySouthern University of Science and TechnologyShenzhen 518055China Department of Rheumatology Guanghua Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Department of Computational Biology St.Jude Children’s Research HospitalMemphisTNUSA Department of Laboratory Medicine Peking University Shenzhen HospitalShenzhenChina Guangdong-Hong Kong-Macao Joint Lab on Chinese Medicine and Immune Disease Research Guangzhou 510006China Shanghai University of Traditional Chinese Medicine Shanghai 200032China State Key Laboratory of Proteomics National Center for Protein Sciences(Beijing)Beijing Institute of Lifeomics100850 BeijingChina

Rheumatoid arthritis(RA)is an autoimmune *** studies hold an opinion that gut microbiota is environmentally acquired and associated with RA ***,accumulating evidence demonstrates that genetics also shape the gut *** is known that some strains of inbred laboratory mice are highly susceptible to collagen-induced arthritis(CIA),while the others are resistant to ***,we show that transplantation of fecal microbiota of CIA-resistant C57BL/6J mice to CIA-susceptible DBA/1J mice confer CIA resistance in DBA/1J mice.C57BL/6J mice and healthy human individuals have enriched *** than DBA/1J mice and RA *** of *** prevents CIA in DBA/1J *** identify that *** mainly produces propionate and C57BL/6J mice and healthy human individuals have higher level of ***-like synoviocytes(FLSs)in RA are activated to undergo tumor-like *** disrupts HDAC3-FOXK1 interaction to increase acetylation of FOXK1,resulting in reduced FOXK1 stability,blocked interferon signaling and deactivation of *** treat CIA mice with propionate and show that propionate attenuates ***,a combination of propionate with anti-TNF etanercept synergistically relieves *** results suggest that *** or propionate could be an alternative or complementary approach to the current therapies.

关键词： HDAC3 cytes interferon