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A non-ACE2-blocking neutralizing antibody against Omicron-included SARS-CoV-2 variants

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Signal Transduction and Targeted Therapy 2022年第2期7卷 372-374页

作者： xiaomin Duan Rui Shi Pulan Liu Qingrui Huang Fengze Wang Xinyu Chen Hui Feng Weijin Huang junyu xiao Jinghua Yan CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking-Tsinghua Center for Life SciencesBeijing Advanced Innovation Center for GenomicsPeking UniversityBeijing 100871China Shanghai Junshi Biosciences Co.Ltd Shanghai 200126China Division of HIV/AIDS and Sextransmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China

Dear Editor,The emergence of severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)variants threatens efforts to contain the coronavirus disease 2019(COVID-19)pandemic.Omicron(B.1.1.529),the fifth novel SARS-CoV-2 variant of concern(VOC),harbors 15 mutations in the receptor-binding domain(RBD)of the spike(S)protein.1 These mutations include almost all the sites of existing VOCs(Alpha/B.1.1.7,Beta/B.1.351,Gamma/P.1,and Delta/B.1.617.2).2–4 Importantly,the key mutations directly interact with the ACE2 receptor and constitute the main target of neutralizing antibodies(NAbs),which is believed to alter the sensitivity to a large number of NAbs.

关键词： ACE2 acute neutral

Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry

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Science China(Life Sciences) 2022年第4期65卷 701-717页

作者： Shiyou Zhu Ying Liu Zhuo Zhou Zhiying Zhang Xia xiao Zhiheng Liu Ang Chen xiaojing Dong Feng Tian Shihua Chen Yiyuan Xu Chunhui Wang Qiheng Li Xuran Niu Qian Pan Shuo Du junyu xiao Jianwei Wang Wensheng Wei Biomedical Pioneering Innovation Center Beijing Advanced Innovation Center for GenomicsPeking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking UniversityBeijing100871China State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing Advanced Innovation Center for GenomicsPeking UniversityBeijing 100871China NHC Key Laboratory of Systems Biology of Pathogens and Christophe Merieux Laboratory Institute of Pathogen BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeKey Laboratory of Respiratory Disease PathogenomicsChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Academy for Advanced Interdisciplinary Studies Peking UniversityBeijing 100871China

The outbreak of coronavirus disease 2019(COVID-19) caused by SARS-CoV-2 has created a global health crisis. SARS-CoV-2 infects varieties of tissues where the known receptor ACE2 is low or almost absent, suggesting the existence of alternative viral entry pathways. Here, we performed a genome-wide barcoded-CRISPRa screen to identify novel host factors that enable SARS-CoV-2 infection. Beyond known host proteins, i.e., ACE2, TMPRSS2, and NRP1, we identified multiple host components,among which LDLRAD3, TMEM30A, and CLEC4G were confirmed as functional receptors for SARS-CoV-2. All these membrane proteins bind directly to spike’s N-terminal domain(NTD). Their essential and physiological roles have been confirmed in either neuron or liver cells. In particular, LDLRAD3 and CLEC4G mediate SARS-CoV-2 entry and infection in an ACE2-independent fashion. The identification of the novel receptors and entry mechanisms could advance our understanding of the multiorgan tropism of SARS-CoV-2, and may shed light on the development of COVID-19 countermeasures.

关键词： CRISPRa screen SARS-CoV-2 novel receptors

Transcriptome analyses of insect cells to facilitate baculovirus-insect expression

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Protein & Cell 2016年第5期7卷 373-382页

作者： Kai Yu Yang Yu xiaoyan Tang Huimin Chen junyu xiao xiao-Dong Su Biodynamic Optical Imaging Center School of Life Science Peking University Beijing 100871 China State Key Laboratory of Protein and Plant Gene Research Peking University Beijing 100871 China Peking-Tsinghua Center for Life Sciences Peking University Beijing 100871 China

The High Five cell line （BTI-TN-5B1-4） isolated from the cabbage looper, Trichoplusia ni is an insect cell line widely used for baculovirus-mediated recombinant protein expression. Despite its widespread application in industry and academic laboratories, the genomic background of this cell line remains unclear. Here we sequenced the transcriptome of High Five cells and assembled 25,234 transcripts. Codon usage analysis showed that High Five cells have a robust codon usage capacity and therefore suit for expressing proteins of both eukaryotic- and prokaryotic-origin. Genes involved in glycosylation were profiled in our study, providing guidance for engineering glycosylated proteins in the insect cells. We also predicted signal peptides for transcripts with high expression abundance in both High Five and Sf21 cell lines, and these results have important implications for optimizing the expression level of some secretory and membrane proteins.

关键词： High Five cell line baculovirus-insect cellsystem codon usage glycosylation signal peptide

不同能流密度下中等能量发散式冲击波治疗跖筋膜炎的短期疗效分析

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中国康复医学杂志 2024年第5期39卷 681-686页

作者：肖健朱俊宇闫君李浩胡帆贾海光梁豪君邢更彦安徽医科大学武警总医院临床学院安徽省合肥市230032 中国人民解放军总医院第三医学中心

目的:分析不同能流密度下中等能量发散式冲击波对跖筋膜炎的短期影响。方法:回顾性分析2017年1月—2021年10月经中等能量发散式冲击波治疗的213例跖筋膜炎患者的临床资料,按0.12m J/mm^(2)治疗者73例(A组);0.14 m J/mm^(2)治疗者71例(B... 详细信息

目的:分析不同能流密度下中等能量发散式冲击波对跖筋膜炎的短期影响。方法:回顾性分析2017年1月—2021年10月经中等能量发散式冲击波治疗的213例跖筋膜炎患者的临床资料,按0.12m J/mm^(2)治疗者73例(A组);0.14 m J/mm^(2)治疗者71例(B组);0.16 m J/mm^(2)治疗者69例(C组)。评价指标为治疗后第4周疼痛视觉模拟评分(visual analogue scale,VAS)及美国矫形外科足踝协会(American Orthopedic Foot and Ankle Society Score,AOFAS)踝—后足功能评分较治疗前的变化;通过治疗前后AOFAS评分差值评价组间疗效差异。建立回归模型探究疗效优良的相关因素。结果:三组在性别、年龄、单双侧、患病时间及BMI无显著性差异,所有患者治疗后无不良反应。三组患者的VAS和AOFAS评分治疗前后均有显著改善(P<0.001);组间AOFAS评分的差值有显著性差异(P<0.05)。逻辑回归表明:年龄(OR=0.946,P<0.05)和患足的单双侧(OR=0.102,P<0.05)可能是影响疗效的危险因素。结论:中等能量发散式冲击波治疗跖筋膜炎能在短期内取得较好疗效,但能流密度(0.12—0.16m J/mm^(2))可能不是决定疗效的关键因素。

关键词：跖筋膜炎冲击波能流密度

3,3',4,4'-联苯四胺改性邻苯二甲腈树脂及其复合材料性能

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高分子材料科学与工程 2024年第2期40卷 65-73页

作者：董俊宇赵星诺章宇琳刘小僮王文蓓周权吴霄华东理工大学材料科学与工程学院特种功能高分子材料及相关技术教育部重点实验室上海200237 中国航空工业集团公司济南特种结构研究所山东济南250023

采用3,3',4,4'-联苯四胺(LBS)与联苯型邻苯二甲腈树脂(BPh)进行预聚反应制备改性联苯型邻苯二甲腈树脂预聚物(BLBS)。利用红外光谱法、差示扫描量热仪、旋转流变仪和热重分析对BLBS的固化行为、流变性能和耐温性能进行研究。结果表明,B... 详细信息

采用3,3',4,4'-联苯四胺(LBS)与联苯型邻苯二甲腈树脂(BPh)进行预聚反应制备改性联苯型邻苯二甲腈树脂预聚物(BLBS)。利用红外光谱法、差示扫描量热仪、旋转流变仪和热重分析对BLBS的固化行为、流变性能和耐温性能进行研究。结果表明,BLBS最低可在240℃前开始固化,但仍需要较高的温度才能固化完全;BLBS-3固化物在氮气和空气氛围中的质量损失5%时的温度(Td5)分别为549.4℃和555.6℃。石英纤维增强BLBS复合材料(QF/BLBS)具有优异的力学性能和耐热性能,玻璃化转变温度(T_(g))大于500°C,室温弯曲强度和层间剪切强度分别为711.5 MPa和48.5 MPa,400℃热处理2 h后弯曲强度和层间剪切强度分别为680.5 MPa和31.3 MPa。

关键词：联苯型邻苯二甲腈 3,3',4,4'-联苯四胺耐热性能力学性能复合材料

Structure of unliganded membrane-proxima domains FN4-FN5-FN6 of DCC

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Protein & Cell 2017年第9期8卷 701-705页

作者： Lorenzo I. Finci Jie Zhang Xiaqin Sun Robert G. Smock Rob Meijers Yan Zhang junyu xiao Jia-huai Wang State Key Laboratory of Biomembrane and Membrane Biotechnology College of Life Sciences Peking University Dana-Farber Cancer Institute Harvard Medical School Boston MA 02215 USA European Molecular Biology Laboratory (EMBL) Hamburg Out-station Hamburg Germany PKU-IDG/McGovern Institute for Brain Research Peking University Beijing 100871 China State Key Laboratory of Protein and Plant Gene Research School of Life Sciences and Peking-Tsinghua Center for Life Sciences Peking University Beijing 100871 China Beijing Advanced Innovation Center for Struc tural Biology Tsinghua-Peking Center for Life Sciences School of Life Sciences and School of Medicine Tsinghua University Beijing 100084 China The Francis Crick Institute London NW12BE UK

Dear Editor, Deleted in colorectal cancer （DCC） is a single pass transmembrane glycoprotein that was originally identified in humans as a candidate tumor suppressor （Fearon et al., 1990）. DCC belongs to the immunoglobulin superfamily, and the extracellular fragment is composed of four immunoglobu- lin-like （Ig-like） domains followed by six fibronectin type III （FN） domains. DCC plays a pivotal role in axon guidance by mediating a combination of attractive and repulsive effects through interactions with the diffusible guidance cue,

关键词： AXON GUIDANCE RECEPTOR DCC NETRIN-1 ASSOCIATION MECHANISM INSIGHT DRAXIN GENE

柔性水凝胶细胞筛药基底评估清热凉血中药复方的抗菌效果

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中国兽医学报 2024年第3期44卷 587-594页

作者：吕若一王潇穆君宇司晓慧孙志刚刘晓晔北京农学院、兽医学(中医药)、北京市重点实验室、北京市中兽药工程技术研究中心北京昌平102206 北京农学院动物科学技术学院北京昌平102206

抗菌药物的筛选技术对于兽医临床合理精准制定抗菌策略至关重要,传统的筛药技术较多关注药物和病原菌、细胞之间的相互作用,没有考虑到宿主体内微环境对药物、细菌和细胞的影响,所以在筛选具有多靶点宿主导向特点的中药复方时参考性较... 详细信息

抗菌药物的筛选技术对于兽医临床合理精准制定抗菌策略至关重要,传统的筛药技术较多关注药物和病原菌、细胞之间的相互作用,没有考虑到宿主体内微环境对药物、细菌和细胞的影响,所以在筛选具有多靶点宿主导向特点的中药复方时参考性较低。用柔性水凝胶在体外重塑细胞生存的微环境模型,筛选4种经典多靶点中药复方的抗菌效果。利用聚丙烯酰胺凝胶、结合微图案复刻的方法,建立类组织细胞微环境的水凝胶,在水凝胶上形成细胞培养芯片,建立金黄色葡萄球菌侵染细胞的感染互作模型,之后加入中药复方治疗。通过荧光技术可视化检测细胞、细菌的数量变化,实现对中药复方可视化和定量化的药效评价。结果显示,牛角地黄汤、清营汤和清瘟败毒饮均有显著的抗菌作用,其中清瘟败毒饮的抗菌效果最佳且对细胞损伤较小,而白头翁汤对细胞毒性较大且抗菌作用不显著。

关键词：水凝胶基底抗菌中药复方细胞筛药方法可视化定量化处理

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聚乳酸对肉鸡生长性能和肠道功能的影响

饲料工业 2024年第5期45卷 32-38页

作者：阿依姑·苏来曼白俊宇武慧敏胡忠泽付松肖徽胡全喜安徽科技学院动物科学学院安徽凤阳233100 动物营养调控与健康安徽省重点实验室安徽凤阳233100 安徽丰原福泰来聚乳酸有限公司安徽蚌埠233000

试验旨在研究聚乳酸(PLA)对AA肉鸡生长性能、肠道功能的影响。选取192只1日龄AA肉鸡随机分成4组,对照组饲喂基础饲粮,试验1、2、3组分别在基础饲粮中添加0.5%、1.0%、2.0%PLA,试验期42 d。分别于肉鸡21、42日龄测定了肉鸡生长性能、肠... 详细信息

试验旨在研究聚乳酸(PLA)对AA肉鸡生长性能、肠道功能的影响。选取192只1日龄AA肉鸡随机分成4组,对照组饲喂基础饲粮,试验1、2、3组分别在基础饲粮中添加0.5%、1.0%、2.0%PLA,试验期42 d。分别于肉鸡21、42日龄测定了肉鸡生长性能、肠道屏障功能、肠道消化酶活性、小肠组织结构、小肠食糜pH和盲肠微生物。结果表明:①与对照组相比,1~21日龄时,1组平均日采食量(ADFI)显著升高(P0.05);③2、3组21日龄的十二指肠胰蛋白酶活性显著升高(P<0.05),1、2组42日龄的十二指肠淀粉酶(AMS)活性显著升高(P<0.05);④1、2组21日龄的十二指肠绒毛高度(VH)显著升高(P<0.05),3组21日龄的空肠绒毛高度/隐窝深度(V/C)显著升高(P<0.05),2组21日龄的回肠VH显著升高(P<0.05),2、3组42日龄十二指肠VH、V/C显著升高(P<0.05),1、2组42日龄空肠VH显著升高(P<0.05);⑤1、2组21日龄的空肠pH显著降低(P<0.05),1、2组42日龄的十二指肠pH显著降低(P<0.05);⑥2组21日龄的盲肠乳酸杆菌、双歧杆菌数量显著升高(P<0.05),1、2组42日龄的盲肠乳酸杆菌数量显著升高(P<0.05)。综上所述,饲粮中添加PLA可以提高肉鸡十二指肠消化酶活性,降低肠道pH,促进有益菌群繁殖,且以添加1.0%PLA效果显著。

关键词：聚乳酸肉鸡生长性能肠道功能消化酶活性

Enlightenment from the COVID-19 Pandemic:The Roles of Environmental Factors in Future Public Health Emergency Response

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Engineering 2022年第1期8卷 108-115页

作者： xiaolei Wang Fengchang Wu xiaoli Zhao xiao Zhang junyu Wang Lin Niu Weigang Liang Kenneth Mei Yee Leung John P.Giesy State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China State Key Laboratory of Marine Pollution&Department of Chemistry City University of Hong KongHong KongChina Department of Veterinary Biomedical Sciences and Toxicology Centre University of SaskatchewanSaskatoonSK S7N 5E2Canada Department of Environmental Sciences Baylor UniversityWacoTX 76798USA

The coronavirus disease 2019(COVID-19)pandemic is challenging the current public health emergency response systems(PHERSs)of many countries.Although environmental factors,such as those influencing the survival of viruses and their transmission between species including humans,play important roles in PHERSs,little attention has been given to these factors.This study describes and elucidates the roles of environmental factors in future PHERSs.To improve countries’capability to respond to public health emergencies associated with viral infections such as the COVID-19 pandemic,a number of environmental factors should be considered before,during,and after the responses to such emergencies.More specifically,to prevent pandemic outbreaks,we should strengthen environmental and wildlife protection,conduct detailed viral surveillance in animals and hotspots,and improve early-warning systems.During the pandemic,we must study the impacts of environmental factors on viral behaviors,develop control measures to minimize secondary environmental risks,and conduct timely assessments of viral risks and secondary environmental effects with a view to reducing the impacts of the pandemic on human health and on ecosystems.After the pandemic,we should further strengthen surveillance for viruses and the prevention of viral spread,maintain control measures for minimizing secondary environmental risks,develop our capability to scientifically predict pandemics and resurgences,and prepare for the next unexpected resurgence.Meanwhile,we should restore the normal life and production of the public based on the“One Health”concept,that views global human and environmental health as inextricably linked.Our recommendations are essential for improving nations’capability to respond to global public health emergencies.

关键词： Public health emergency Public health emergency response system Environmental factors Prevention and control Viral infections