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Co-culture with cardiomyocytes induces mesenchymal stem cells to differentiate into cardiomyocyte-like cells and express heart development-associated genes

Co-culture with cardiomyocytes induces mesenchymal stem cell...

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第一届全国中西医结合心血管病中青年医师论坛

作者： junyi zeng~1 yang wang~2 yunfeng wei~1 an xie~2 yuanlei lou~2 mei zhang~1 1 department of cardiology, 2 institute of urological surgery,the first affiliated hospital of nanchang university,nanchang 330006

Recent studies have demonstrated that bone marrow mesenchymal stem cells(MSCs)have the potential to transdifferentiate into cardiomyocytes(CMs)in an appropriate condition both in vivo and in vitro,and our previous studies also confirmed ***,there exists a controversy among a variety of studies about whether MSCs can differentiate into CMs without physical contacts with CMs,and the mo- lecular signals that underlie this process are not fully *** the present study,MSCs isolated from adult rats were cocuhured with CMs obtained from neonatal rat ventricles at 1:5 ratio in a dual chamber dish separated by a semipermeable membrane for 2 *** this non-direct contact cocuhure procedure,cardiomyogenic differentiation and relative genes expression in MSCs were evalua- *** coculture with CMs,MSCs showed a stick-like or elliptical morphology,lmmunofluorescent stain results revealed that α-aetin and cardiac troponin T(cTnT)positive cells were found in MSCs at 5 days after cocuhure with CMs,and 29.63% of MSCs were positive for α-actin and 27.38% for cTnT at 14 *** from RT-PCR demonstrated the expression level of TGF-β,Nkx-2.5,GATA-4 and MEF-2C genes began to increase at 1 day and reached the peak at 7 days after *** conclu- sion,non-direct contact with CMs(1:5)is conducive for MSCs to differentiate into CMs in vitro,and TGF-β,Nkx-2.5,GATA-4 and MEF-2C genes may play a crucial role during the transdifferentia- tion.

关键词： bone marrow mesenchymal stem cells non-direct contact differentiation genes cardiomyocytes

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Co-culture with cardiomyocytes induces mesenchymal stem cells to differentiate into cardiomyocyte-like cells and express heart development-associated genes

Co-culture with cardiomyocytes induces mesenchymal stem cell...

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江西省第四次中西医结合心血管学术交流会

作者： junyi zeng~1,yang wang~2,yunfeng wei~1,An xie~2,yuanlei lou~2,mei zhang~1 1 department of cardiology,2 institute of urological surgery,the first affiliated hospital of nanchang university

＜正＞Recent studies have demonstrated that bone marrow mesenchymal stem cells（MSCs） have the potential to transdifferentiate into cardiomyocytes（CMs） in an appropriate condition both in vivo and in vitro,and our previous studies also confirmed ***,there exists a controversy among a variety of studies about whether MSCs can differentiate into CMs without physical contacts with CMs,and the molecular signals that underlie this process are not fully *** the present study,MSCs isolated from adult rats were cocultured with CMs obtained from neonatal rat ventricles at 1:5 ratio in a dual chamber dish separated by a semipermeable membrane for 2 *** this non-direct contact coculture procedure,cardiomyogenic differentiation and relative genes expression in MSCs were evaluated. After coculture with CMs,MSCs showed a stick-like or elliptical *** stain results revealed that a -actin and cardiac troponin T（cTnT） positive cells were found in MSCs at 5 days after coculture with CMs,and 29.63%of MSCs were positive forα-actin and 27.38%for cTnT at 14 days. Results from RT-PCR demonstrated the expression level of TGF-β,Nkx-2.5,GATA-4 and MEF-2C genes began to increase at 1 day and reached the peak at 7 days after *** conclusion,non-direct contact with CMs（1:5） is conducive for MSCs to differentiate into CMs in vitro,and TGF-β,Nkx-2.5, GATA-4 and MEF-2C genes may play a crucial role during the transdifferentiation.

关键词： bone marrow mesenchymal stem cells non - direct contact differentiation genes cardiomyocytes

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2021 Chinese consensus on the diagnosis and management of primary immune thrombocytopenia in pregnancy

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Chinese Medical Journal 2022年第8期135卷 887-889页

作者： zhang Xiaohui Chen Fangping Chen xiequn Cheng yunfeng Fang meiyun Feng Jianming Fu Haixia Gao Hong Han Yue He Aili Hou Ming Hu Yu Huang Ruibin Huang Wenrong Jing Zhicheng Kong Peiyan Liang Aibin Liang meiying Liu Daihong Liu Junling Liu Lin Liu Xiaowei Ma Liangming mei Heng Ni Heyu Niu Ting Peng Jun Qiao Jianlin Ren Jinhai Song Yongping Tang Liang V Tong Tong wang Shaoyuan wang Xin wang Zhao wei Hui Wu Depei Wu Guangsheng Xu Caigang Xu Xue Xu Yajing yang Linhua yang Renchi yang Tonghua Yin Chenghong Yu Li zhang Guangsen zhang Lei zhang Liansheng zhang Xi Zhao weili Zhao Yongqiang Zhou Daobin Zhou Hu Zhou Zeping Zhu Tienan wang Jianliu Huang Xiaojun department of Hematology Peking University People’s HospitalPeking University Institute of HematologyBeijing 100044China department of Hematology Xiangya Third HospitalCentral South UniversityChangshaHunan 410013China department of Hematology Xi Jing Hospital Affiliated to the Fourth Military Medical UniversityXi’anShaanxi 710032China department of Hematology Qingpu Branch of Zhongshan HospitalFudan UniversityShanghai 528404China department of Hematology Affiliated Zhongshan Hospital of Dalian UniversityDalianLiaoning 116001China department of Hematology and Rheumatology Qinghai Provincial People’s HospitalXiningQinghai 810007China department of Internal Medicine Beijing Obstetrics and Gynecology HospitalCapital Medical UniversityBeijing Maternal and Child Health Care HospitalBeijing 100026China department of Hematology Jiangsu Institute of HematologyNational Clinical Research Center for Hematologic DiseasesThe First Affiliated Hospital of Soochow UniversitySuzhouJiangsu 215006China department of Hematology The Second Affiliated HospitalMedical School of Xi’an Jiaotong UniversityXi’anShaanxi 710004China department of Hematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinanShandong 250063China department of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China department of Hematology The First Affiliated Hospital of Nanchang UniversityNanchangJiangxi 330006China department of Hematology Chinese PLA General HospitalMedical School of Chinese PLABeijing 100039China department of cardiology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China department of Hematology Medical Center of HematologyXinqiao HospitalThe Third Military Medical UniversityChongqing 400037China department of Hematology Tongji Hospital of Tongji UniversityShanghai 200092China department of Obstetrics and Gynecology Peking University People

Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during *** with ITP who have severe thrombocytopenia are at an increased risk for life-threatening obstetric ***,we established this consensus statement on the diagnosis and management of ITP during pregnancy(detailed information is available in the Supplementary File,http://***7CM9/A978).

关键词： diagnosis pregnancy ITP

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双荧光素酶报告基因技术验证miR-31对LATS2的靶向调控作用

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检验医学与临床 2019年第15期16卷 2113-2116,2119页

作者：谢飞曾俊义张婉魏云峰郑泽琪丁露文通易达松南昌大学第一附属医院心血管内科江西南昌330006 南昌大学第一附属医院江西省高血压病研究所江西南昌330006

目的通过双荧光素酶报告基因技术验证miR-31对大肿瘤抑制因子2(LATS2)的靶向调控作用,观察miR-31在心肌细胞肥大过程中的可能影响。方法运用Targetscan软件预测大鼠miR-31对LATS2的靶向调控作用;以双荧光素酶报告质粒为工具载体,分别插... 详细信息

目的通过双荧光素酶报告基因技术验证miR-31对大肿瘤抑制因子2(LATS2)的靶向调控作用,观察miR-31在心肌细胞肥大过程中的可能影响。方法运用Targetscan软件预测大鼠miR-31对LATS2的靶向调控作用;以双荧光素酶报告质粒为工具载体,分别插入人工合成的大鼠LATS2基因野生型3′UTR(LATS2-3′UTR-WT)及突变型3′UTR(LATS2-3′UTR-MU)片段,构建LATS2-3′UTR-WT及LATS2-3′UTR-MU双荧光素酶报告质粒;重组双荧光素酶报告质粒分别与miR-31过表达质粒共转染293T细胞,检测荧光素酶活性并分析miR-31对LATS2的靶向调控作用;血管紧张素Ⅱ(AngⅡ)诱导体外心肌细胞肥大模型,RT-qPCR检测miR-31、LATS2及心肌细胞肥大基因心房钠尿肽(ANP)、β-肌球蛋白重链(β-MHC)的表达,F肌动蛋白荧光探针观察心肌细胞形态变化。结果Targetscan软件预测结果显示,大鼠miR-31与LATS2基因3′UTR存在互补结合位点;经酶切及基因测序鉴定,成功构建LATS2-3′UTR-WT及LATS2-3′UTR-MU双荧光素酶报告质粒;与LATS2-3′UTR-MU+miR-31组相比,LATS2-3′UTR-NC+miRNA-31组荧光素酶活性无明显变化(0.98±0.03vs.1.00±0.03,P>0.05),而LATS2-3′UTR-MT+miR-31组与LATS2-3′UTR-NC+miR-31组相比,荧光素酶活性显著降低(0.74±0.02vs.1.00±0.03,P1);AngⅡ诱导48h后可检测到心肌细胞肥大基因ANP(P1)及β-MHC表达上调(P1表达显著上调(P1),LATS2基因明显下调(P1可通过与LATS2基因3′UTR互补结合实现其对LATS2靶向调控作用,miR-31靶向作用LATS2可能参与调控心肌细胞的肥大。

关键词：双荧光素酶报告基因微小核糖核酸-31 大肿瘤抑制因子2 靶向调控心肌细胞肥大

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原发性高血压左室肥厚患者循环miRNA的高通量测序分析

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临床心血管病杂志 2018年第6期34卷 549-555页

作者：曾俊义易达松张婉魏云峰郑泽琪彭景添丁露郑耀富高智明南昌大学第一附属医院江西省高血压病研究所南昌330006 南昌大学第一附属医院心血管内科

目的:探讨原发性高血压左室肥厚患者循环miRNA的特异性表达,为高血压左室肥厚的诊断和预测寻找新的候选生物学标志。方法:入选原发性高血压合并左室肥厚及原发性高血压不合并左室肥厚患者各5例,采集患者血浆标本并进行miRNA提取,通过高... 详细信息

目的:探讨原发性高血压左室肥厚患者循环miRNA的特异性表达,为高血压左室肥厚的诊断和预测寻找新的候选生物学标志。方法:入选原发性高血压合并左室肥厚及原发性高血压不合并左室肥厚患者各5例,采集患者血浆标本并进行miRNA提取,通过高通量二代测序技术检测两组患者循环miRNA表达变化,对测序数据进行分析筛选出差异性表达miRNA,进一步通过生物信息学分析差异表达miRNA的靶基因并进行功能富集分析,初步探讨差异表达miRNA的功能。结果:筛选出原发性高血压左室肥厚患者差异化特异性表达miRNA共64个,其中表达上调的35个,表达下调的29个。生物信息学分析显示差异性表达miRNA的功能主要涉及心肌细胞肾上腺素信号、神经活化配体/受体相互作用、免疫炎症反应、脂肪酸代谢等与高血压左室肥厚密切相关信号通路的调控。变化差异最大的10个miRNA中,hsa-mir-100、hsa-mir-202、hsa-mir-181a-2、hsa-mir-450b富集的功能最为显著。结论:原发性高血压左室肥厚患者外周循环中存在特异性miRNA表达谱,可为原发性高血压左室肥厚的诊断和预测提供候选生物学标志。

关键词：原发性高血压左室肥厚循环miRNA 高通量测序

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