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胜利油田钻井液环保处理技术研究与应用

石油钻探技术 2024年第3期52卷 47-52页

作者：刘均一李公让黄利民马晓勇夏晔中石化胜利石油工程有限公司钻井工艺研究院山东东营257100 中石化胜利石油工程有限公司黄河钻井总公司山东东营257513

在胜利油田钻井过程中,废弃物总量大、污染物含量高,废弃物污染控制集中于末端治理,资源化利用程度低、成本高,无法实现钻井过程中的污染控制,严重影响油田生态环境。针对该油田钻井过程中的环保关键技术难题,以“减量化、无害化、资源... 详细信息

在胜利油田钻井过程中,废弃物总量大、污染物含量高,废弃物污染控制集中于末端治理,资源化利用程度低、成本高,无法实现钻井过程中的污染控制,严重影响油田生态环境。针对该油田钻井过程中的环保关键技术难题,以“减量化、无害化、资源化”为目标,进行了理论研究、装备研发、技术攻关和集成应用,形成了钻井污染物源头控制、全井段钻井废弃物随钻过程减量化和钻井废弃物末端无害化处理与资源化利用等3项核心技术,创新了覆盖“源头控制、过程减量、末端治理”的钻井液环保处理技术。“十三五”期间,该钻井液环保处理技术在胜利油区及新疆、四川等地的中国石化工区实现规模化应用,建成了胜利油田滨105、盐222、桩146等绿色低碳钻井示范工程,为保障胜利油田绿色低碳开发提供了技术支撑,也为我国陆上油气田绿色低碳开发提供了有益借鉴。

关键词：钻井液环境保护废弃物处理固液分离资源化利用胜利油田

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Stem cell niche-inspired microcarriers with ADSCs encapsulation for diabetic wound treatment

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Bioactive Materials 2023年第8期26卷 159-168页

作者： Xiangyi Wu Haofang Zhu junyi Che ye Xu Qian Tan Yuanjin Zhao Department of Burns and Plastic Surgery Institute of Translational MedicineThe Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing210002China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhouZhejiang325000China

Stem cell therapies have made great progress in the treatment of diabetic wounds during recent decades,while their short in vivo residence,alloimmune reactions,undesired behaviors,and dramatic losses of cell functions still hinder the translation of them into clinic.Here,inspired by the natural components of stem cell niches,we presented novel microfluidic hydrogel microcarriers with extracellular matrix(ECM)-like composition and adipose-derived stem cells(ADSCs)encapsulation for diabetic wound healing.As the hydrogel was synthesized by conjugating hyaluronic acid methacryloyl(HAMA)onto the Fibronectin(FN)molecule chain(FN-HAMA),the laden ADSCs in the microcarriers showed improved bioactivities and pro-regenerative capabilities.Based on these features,we have demonstrated that these ADSCs microcarriers exhibited significant promotion of neovascularization,follicular rejuvenation,and collagen deposition in a mouse diabetic wound model.These results indicated that the stem cell niche-inspired FN-HAMA microcarriers with ADSCs encapsulation have great clinical potential for diabetic wound treatment.

关键词： Stem cell Bio-inspired Microfluidics Microcarrier Wound healing Hydrogel

stAPAminer:Mining Spatial Patterns of Alternative Polyadenylation for Spatially Resolved Transcriptomic Studies

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Genomics, Proteomics & Bioinformatics 2023年第3期21卷 601-618页

作者： Guoli Ji Qi Tang Sheng Zhu junyi Zhu Pengchao ye Shuting Xia Xiaohui Wu Pasteurien College Suzhou Medical College of Soochow UniversitySoochow UniversitySuzhou 215000China Department of Automation Xiamen UniversityXiamen 361005China Institute of Neuroscience Soochow UniversitySuzhou 215000China

Alternative polyadenylation(APA)contributes to transcriptome complexity and gene expression regulation and has been implicated in various cellular processes and diseases.Singlecell RNA sequencing(scRNA-seq)has enabled the profiling of APA at the single-cell level;however,the spatial information of cells is not preserved in scRNA-seq.Alternatively,spatial transcriptomics(ST)technologies provide opportunities to decipher the spatial context of the transcriptomic landscape.Pioneering studies have revealed potential spatially variable genes and/or splice isoforms;however,the pattern of APA usage in spatial contexts remains unappreciated.In this study,we developed a toolkit called stAPAminer for mining spatial patterns of APA from spatially barcoded ST data.APA sites were identified and quantified from the ST data.In particular,an imputation model based on the k-nearest neighbors algorithm was designed to recover APA signals,and then APA genes with spatial patterns of APA usage variation were identified.By analyzing wellestablished ST data of the mouse olfactory bulb(MOB),we presented a detailed view of spatial APA usage across morphological layers of the MOB.We compiled a comprehensive list of genes with spatial APA dynamics and obtained several major spatial expression patterns that represent spatial APA dynamics in different morphological layers.By extending this analysis to two additional replicates of the MOB ST data,we observed that the spatial APA patterns of several genes were reproducible among replicates.stAPAminer employs the power of ST to explore the transcriptional atlas of spatial APA patterns with spatial resolution.This toolkit is available at https://***/BMILAB/stAPAminer and https://***/biocode/tools/BT007320.

关键词： Alternative polyadenylation Spatial transcriptomics Single-cell RNA sequencing Spatial pattern Imputation

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运用一站式智能导医平台改善患者就医体验

中国卫生质量管理 2024年第5期31卷 54-57页

作者：陈烨袁骏毅侯旭敏彭红上海市胸科医院/上海交通大学医学院附属胸科医院上海200030 上海交通大学医院发展研究院医疗服务管理研究所上海200030

智能导医是医院智慧服务的重要组成部分。上海市胸科医院借助事件驱动和人工智能技术,以微信公众号为载体,构建了一站式智能导医平台,根据不同患者门诊就医需求,制订个体化就医规划,以任务流形式串联各就诊环节,缩短了患者候诊时间,缓... 详细信息

智能导医是医院智慧服务的重要组成部分。上海市胸科医院借助事件驱动和人工智能技术,以微信公众号为载体,构建了一站式智能导医平台,根据不同患者门诊就医需求,制订个体化就医规划,以任务流形式串联各就诊环节,缩短了患者候诊时间,缓解了导医工作压力,改善了患者就医体验。

关键词：门诊智能导医智慧服务就医体验事件驱动技术

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Prevalence and clinical significance of pathogenic germline BRCA1/2 mutations in Chinese non-small cell lung cancer patients

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Cancer Biology & Medicine 2019年第3期16卷 556-564页

作者： Xingsheng Hu Dongyong Yang Yalun Li Li Li Yan Wang Peng Chen Song Xu Xingxiang Pu Wei Zhu Pengbo Deng junyi ye Hanhan Zhang Analyn Lizaso Hao Liu Xinru Mao Hai Huang Qian Chu Chengping Hu Department of Medical Oncology Cancer HospitalChinese Academy of Medical SciencesBeijing100021China Department of Pulmonary Medicine Second Affiliated Hospital of Fujian Medical UniversityQuanzhou362100China Department of Respiratory Medicine West China HospitalSichuan UniversityChengdu610041China Department ofRespiratory Medicine Daping HospitalChongqing400042China Department of Respiratory Medicine Xinqiao HospitalArmy Medical UniversityChongqing400037China Department of Medical Oncology Tianjin Medical University CancerInstitute and HospitalNational Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin Tianjin's Clinical Research Center for Cancer Tianjin300060China Department of Thoracic Oncology TianjinMedical University General HospitalTianjin300052China Department of Medical Oncology Hunan Cancer HospitalChangsha410006China Department of Pathology Xiangya HospitalCentral South UniversityChangsha410008China Department of Respiratory Medicine Xiangya HospitalCentral South UniversityChangsha410008China Burning RockBiotech Guangzhou510300China Department of Oncology Tongji Hospital of Tongji Medical CollegeHuazhongUniversity of Science and TechnologyWuhan430022China

Objective: Germline alterations in the breast cancer susceptibility genes type 1 and 2, BRCA1 and BRCA2, predispose individuals to hereditary cancers, including breast, ovarian, prostate, pancreatic, and stomach cancers.Accumulating evidence suggests inherited genetic susceptibility to lung cancer.The present study aimed to survey the prevalence of pathogenic germline BRCA mutations(gBRCAm) and explore the potential association between gBRCAm and disease onset in Chinese advanced non-small cell lung cancer(NSCLC) patients.Methods: A total of 6,220 NSCLC patients were screened using capture-based ultra-deep targeted sequencing to identify patients harboring germline BRCA1/2 mutations.Results: Out of the 6,220 patients screened, 1.03%(64/6,220) of the patients harbored the pathogenic gB RCAm, with BRCA2 mutations being the most predominant mutations(49/64, 76.5%).Patients who developed NSCLC before 50 years of age were more likely to carry gBRCAm(P = 0.036).Among the patients harboring classic lung cancer driver mutations, those with concurrent gBRCAm were significantly younger than those harboring the wild-type gBRCA(P = 0.029).By contrast, the age of patients with or without concurrent gBRCAm was comparable to those of patients without the driver mutations(P = 0.972).In addition, we identified EGFR-mutant patients with concurrent gBRCAm who showed comparable progression-free survival but significantly longer overall survival(P = 0.002) compared to EGFR-mutant patients with wild-type germline BRCA.Conclusions: Overall, our study is the largest survey of the prevalence of pathogenic gBRCAm in advanced Chinese NSCLC patients.Results suggested a lack of association between germline BRCA status and treatment outcome of EGFR-TKI.In addition,results showed a positive correlation between pathogenic gB RCAm and an early onset of NSCLC.

关键词： Germline BRCA mutations non-small cell lung cancer prevalence BRCA1 BRCA2

Anisodine hydrobromide alleviates oxidative stress caused by hypoxia/reoxygenation in human cerebral microvascular endothelial cells predominantly via inhibition of muscarinic acetylcholine receptor 4

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BIOCELL 2023年第10期47卷 2255-2263页

作者： WENLI JIANG junyi SHEN XIAOQIANG DU YAN QIU JIAN ZHONG ZHI OUYANG BINGMEI M.FU ye ZENG Institute of Biomedical Engineering West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengdu610041China Department of Biomedical Engineering The City College of the City University of New YorkNew York10031USA

Background:Anisodine hydrobromide(AT3),an anti-cholinergic agent,could be delivered to the brain across the blood-brain barrier and has been used clinically for the treatment of cerebral ischemia/reperfusion injury.Endothelial dysfunction can be caused by hypoxia/reoxygenation(H/R)via oxidative stress and metabolic alterations.The present study investigated whether AT3 regulates the production of nitric oxide(NO)and reactive oxygen species(ROS),and the HIF-1αpathway via regulation of muscarinic acetylcholine receptors(mAChRs)in brain microvascular endothelial cells after H/R exposure.Methods:Under H/R conditions,hCMEC/D3 cerebral microvascular endothelial cells were treated with AT3.Specific inhibitors of M2-and M4-mAChRs were used to explore the mechanism by which AT3 influences oxidative stress in endothelial cells.Then,mAChRs expression was detected by western blotting and NO production was detected by Greiss reaction.The intracellular ROS level was measured using DCFH-DA probes.The expression of hypoxia-inducible transcription factor 1α(HIF-1α)was also detected.Results:While H/R induced the expression of M2-and M4-mAChRs,AT3 suppressed the H/R-upregulated M2-and M4-mAChRs.H/R also induced the production of NO,ROS,and apoptosis.AT3 and M4-mAChR inhibitors inhibited the H/R-induced production of NO and ROS and apoptosis.HIF-1αwas induced by H/R,but was suppressed by AT3.Conclusion:Thus,the in vitro evidence shows that AT3 protects against H/R injury in cerebral microvascular endothelial cells via inhibition of HIF-1α,NO and ROS,predominantly through the downregulation of M4-mAChR.The findings offer novel understandings regarding AT3-mediated attenuation of endothelial cell apoptosis and cerebral ischemia/reperfusion injury.

关键词： Hypoxia/reoxygenation Endothelial cell Anisodine hydrobromide Muscarinic acetylcholine receptors Hypoxia-inducible factor-1α

MEX3A对结肠癌细胞恶性表型的影响及其机制研究

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现代肿瘤医学 2024年第2期32卷 226-233页

作者：周俊邑沙晓锋柳叶韩卓莹丁姝黄豪罗超仲小敏徐州医科大学淮安临床学院江苏淮安223300 淮安市洪泽区人民医院肿瘤科江苏淮安223100 南京医科大学附属淮安第一医院肿瘤科中心实验室江苏淮安223300

目的:探讨MEX3A在结肠癌组织中的表达及其对结肠癌细胞恶性表型的影响并探索其机制。方法:TCGA数据库分析MEX3A在结肠癌组织及正常组织中的差异表达及其与患者临床病理分期和预后的相关性,并通过免疫组化进一步验证。采用慢病毒对结肠... 详细信息

目的:探讨MEX3A在结肠癌组织中的表达及其对结肠癌细胞恶性表型的影响并探索其机制。方法:TCGA数据库分析MEX3A在结肠癌组织及正常组织中的差异表达及其与患者临床病理分期和预后的相关性,并通过免疫组化进一步验证。采用慢病毒对结肠癌细胞中MEX3A的表达进行敲低,检测细胞的增殖、凋亡、迁移及侵袭能力。裸鼠皮下荷瘤后对肿瘤生长进行测量记录。Flag融合表达质粒过表达MEX3A后进行COIP实验。采用质谱进行蛋白质定性检测(Shotgun)与MEX3A互作的蛋白质。生物信息学分析后进行COIP验证,对互作蛋白表达进行挽救实验验证。结果:MEX3A在结肠癌组织中表达显著上调且与患者临床病理特征及预后相关。采用慢病毒敲减MEX3A表达后,结肠癌细胞增殖能力显著被抑制,凋亡则显著增加。细胞转移能力检测结果显示,结肠癌细胞的迁移及侵袭能力显著被抑制。小鼠体内实验结果表明,敲减MEX3A表达后,肿瘤在体内的生长明显被抑制。蛋白质定性检测及COIP验证结果显示,EIF2AK2、LAMB3及MSI2与MEX3A蛋白存在相互作用。在敲减MEX3A表达后分别过表达EIF2AK2、LAMB3及MSI2后细胞增殖能力被不同程度恢复,其中MSI2过表达后细胞增殖能力恢复最明显。MTT及细胞迁移能力验证结果显示过表达MSI2后,细胞增殖及迁移能力显著恢复。结论:MEX3A在结肠癌组织中表达上调且与患者预后呈负相关,其通过与MSI2相互作用促进结肠癌细胞的恶性表型。

关键词： MEX3A 结肠癌预后恶性表型

Engineering J-aggregates for NIR-induced meso-CF_(3)-BODIPY nanoparticles by activated apoptosis mechanism in photothermal therapy

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中国化学快报:英文版 2023年第9期34卷 188-192页

作者： Chujing ye Shan Zhang Dongxiang Zhang Yue Shen Zhan Wang Huan Wang junyi Ren Xin-Dong Jiang Jianjun Du Rong Shang Guiling Wang Liaoning&Shenyang Key Laboratory of Functional Dye and Pigment Shenyang University of Chemical TechnologyShenyang 110142China Department of Cell Biology China Medical UniversityShenyang 110122China State Key Laboratory of Fine Chemicals Dalian University of TechnologyDalian 110624China Department of Chemistry Graduate School of ScienceHiroshima UniversityHigashi-Hiroshima 7398526Japan

Forming J-aggregates by organic monomer is a fascinating strategy to urge spectroscopic redshift with respect to that of the monomer.Herein,we designed 1,7-diphenyl-substituted meso–CF_(3)-BDP monomer confirmed by X-ray crystallographic analysis.The low-barrier rotation of the–CF_(3)group in meso–CF_(3)-BDP1 significantly enhances the non-radiative efficiency,and the photothermal conversion efficiency(PCE)of the self-assembled nanoparticles(1-NPs:λ_(abs)=746 nm)by J-aggregates was 82%.1-NPs could effectively block cell cycle progression,inhibit cancer cell proliferation and trigger cell apoptosis under low power laser irradiation(0.2 W/cm^(2)).This study proposes an alternate molecular design platform by J-aggregates to promote PCE through the insertion of rotating segment and trigger the cancer cells apoptosis in photothermal therapy at low power laser density.

关键词： NIR dye J-aggregate CF_(3)-BODIPY Photothermal therapy Cell apoptosis

Numerical analysis on noise of rotor with unconventional blade tips based on CFD/Kirchhoff method

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Chinese Journal of Aeronautics 2013年第3期26卷 572-582页

作者： Wang Bo Zhao Qijun Xu Guohua ye Liang Wang junyi National Key Laboratory of Science and Technology on Rotorcraft Aeromechanics Nanjing University of Aeronautics and Astronautics

A solver is developed aiming at efficiently predicting rotor noise in hover and forward flight. In this solver, the nonlinear near-field solutions are calculated by a hybrid approach which includes the Navier–Stokes and Euler equations based on a moving-embedded grid system and adaptive grid methodology. A combination of the third-order upwind scheme and flux-difference splitting scheme, instead of the second-order center-difference scheme which may cause larger wake dissipation, has been employed in the present computational fluid dynamics （CFD） method. The sound pressure data in the near field can be calculated directly by solving the Navier–Stokes equations, and the sound propagation can be predicted by the Kirchhoff method. A harmonic expansion approach is presented for rotor far-field noise prediction, which gives an analytical expression for the integral function in the Kirchhoff formula. As a result, the interpolation process is simplified and the efficiency and accuracy of the interpolation are improved. Then, the high-speed impulsive （HIS） noise of a helicopter rotor at different tip Mach numbers and on different observers is calculated and analyzed in hover and forward flight, which shows a highly directional characteristic of the rotor HIS noise with a maximum value in the rotor plane, and the HSI noise weakens rapidly with the increasing of the directivity angle. In order to investigate the effects of the rotor blade-tip shape on its aeroacoustic characteristics, four kinds of blade tips are designed and their noise characteristics have been simulated. At last, a new unconventional CLOR-II blade tip has been designed, and the noise characteristics of the presented CLOR-II model rotor have been simulated and measured compared to the reference rotors with a rectangular or swept-back platform blade tip. The results demonstrate that the unconventional CLOR-II blade tip can significantly reduce the HSI noise of a rotor.

关键词： Aeroacoustics Computational fluid dynam- ics Helicopters Kirchhoff Rotors Unconventional blade tip