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中华医学杂志（英文版） 2023年第19期136卷 2362-2364页

作者： Weili Qi junyi shen Yu Zhang Fengwei Gao Chuan Li China 610041 China 610041 China 610072 China 614000

Serum hepatitis B virus deoxyribonucleic acid (HBVDNA) load is a critical index used to reflect the degree of viral replication in hepatocytes and to evaluate host hepatitis viral status. The risk of hepatocellular carcinoma (HCC) incidence or recurrence varies depending on whether the HBV-DNA load is high or low. Consistent with previous studies and expert opinion, a HBV-DNA level >2000 IU/mL was defined as a high HBV-DNA load in the current study.

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Safety of elective hepatectomy performed on weekend for patients with hepatocellular carcinoma

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Chinese Medical Journal 2022年第21期135卷 2596-2598页

作者： Junlong Dai junyi shen Xiaoyun Zhang Wei Peng Tianfu Wen Chuan Li Department of Liver Surgery Liver Transplantation CenterWest China HospitalSichuan UniversityChengduSichuan 610041China

The incidence of hepatocellular carcinoma(HCC)is increasing year by year.The International Agency for Research on Cancer(IARC)reported that more than 630,000 people were diagnosed with HCC in 2020 and more than half of new cases of HCC were in China.The increasing number of patients overburdens the medical system and the waiting time before the surgery becomes longer and longer.This poses a new challenge to the applicability of traditional surgical management model.The elective surgery performed on weekend could provide an efficient solution to the problem.However,the safety of weekend surgery is controversial because surgical patients have a higher severe complication incidence and postoperative mortality on the weekend compared to the weekday(ie,weekend effect).

关键词： patients hepatocellular surgery

Tenofovir vs.entecavir on recurrence of hepatitis B virus-related hepatocellular carcinoma beyond Milan criteria after hepatectomy

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Chinese Medical Journal 2022年第3期135卷 301-308页

作者： junyi shen Weili Qi Junlong Dai Shusheng Leng Kangyi Jiang Yu Zhang Shun Ran Chuan Li Tianfu Wen Department of Liver Surgery and Liver Transplantation Center West China HospitalSichuan UniversityChengduSichuan 610041China Laboratory of Liver Transplantation Frontiers Science Center for Disease-Related Molecular NetworkWest China HospitalSichuan UniversityChengduSichuan 610041China Institute of Clinical Pathology West China HospitalSichuan UniversityChengduSichuan 610041China Department of Hepatobiliary and Pancreatic Surgery The Affiliated Hospital of Chengdu UniversityChengduSichuan 610072China Department of Hepatobiliary and Pancreatic Surgery The People's Hospital of LeshanLeshanSichuan 614700China Department of Hepatobiliary Surgery Sichuan Provincial People's HospitalChinese Academy of SciencesChengduSichuan 610072China Department of Hepatobiliary Surgery Affiliated Cancer Hospital of Guizhou Medical UniversityGuiyangGuizhou 550000China

Background:Hepatectomy for hepatocellular carcinoma(HCC)beyond the Milan criteria is shown to be beneficial.However,a high rate of post-operative HCC recurrence hinders the long-term survival of the patients.This study aimed to investigate and compare the impacts of tenofovir(TDF)and entecavir(ETV)on the recurrence of hepatitis B viral(HBV)-related HCC beyond the Milan criteria.Methods:Data pertaining to 1532 patients who underwent hepatectomy and received antiviral therapy between January 2014 and January 2019 were collected from five centers.Recurrence-free survival(RFS)analysis was performed using the Kaplan-Meier method.Cox proportional hazards regression analysis was performed to determine prognostic factors for HCC recurrence.Results:The analysis incorporates 595 HBV-related HCC patients.The overall 5-year RFS was 21.3%.Among them,533 and 62 patients received ETV and TDF treatment,respectively.The 1-,3-,and 5-year RFS rates were 46.3%,27.4%,and 19.6%,respectively,in the ETV group compared with 65.1%,41.8%,and 37.2%,respectively,in the TDF group(P<0.001).Multivariate analysis showed that TDF treatment(hazard ratio[HR]:0.604,P=0.005),cirrhosis(HR:1.557,P=0.004),tumor size(HR:1.037,P=0.008),microvascular invasion(MVI)(HR:1.403,P=0.002),portal vein tumor thrombus(PVTT)(HR:1.358,P=0.012),capsular invasion(HR:1.228,P=0.040),and creatinine levels(CREA)(HR:0.993,P=0.031)were statistically significant prognostic factors associated with RFS.Conclusions:Patients with HCC beyond the Milan criteria exhibited a high rate of HCC recurrence after hepatectomy.Compared to the ETV therapy,TDF administration significantly lowered the risk of HCC recurrence.

关键词： Hepatocellular carcinoma Hepatitis B virus Antiviral drugs Recurrence Hepatectomy

Anisodine hydrobromide alleviates oxidative stress caused by hypoxia/reoxygenation in human cerebral microvascular endothelial cells predominantly via inhibition of muscarinic acetylcholine receptor 4

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BIOCELL 2023年第10期47卷 2255-2263页

作者： WENLI JIANG junyi shen XIAOQIANG DU YAN QIU JIAN ZHONG ZHI OUYANG BINGMEI M.FU YE ZENG Institute of Biomedical Engineering West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengdu610041China Department of Biomedical Engineering The City College of the City University of New YorkNew York10031USA

Background:Anisodine hydrobromide(AT3),an anti-cholinergic agent,could be delivered to the brain across the blood-brain barrier and has been used clinically for the treatment of cerebral ischemia/reperfusion injury.Endothelial dysfunction can be caused by hypoxia/reoxygenation(H/R)via oxidative stress and metabolic alterations.The present study investigated whether AT3 regulates the production of nitric oxide(NO)and reactive oxygen species(ROS),and the HIF-1αpathway via regulation of muscarinic acetylcholine receptors(mAChRs)in brain microvascular endothelial cells after H/R exposure.Methods:Under H/R conditions,hCMEC/D3 cerebral microvascular endothelial cells were treated with AT3.Specific inhibitors of M2-and M4-mAChRs were used to explore the mechanism by which AT3 influences oxidative stress in endothelial cells.Then,mAChRs expression was detected by western blotting and NO production was detected by Greiss reaction.The intracellular ROS level was measured using DCFH-DA probes.The expression of hypoxia-inducible transcription factor 1α(HIF-1α)was also detected.Results:While H/R induced the expression of M2-and M4-mAChRs,AT3 suppressed the H/R-upregulated M2-and M4-mAChRs.H/R also induced the production of NO,ROS,and apoptosis.AT3 and M4-mAChR inhibitors inhibited the H/R-induced production of NO and ROS and apoptosis.HIF-1αwas induced by H/R,but was suppressed by AT3.Conclusion:Thus,the in vitro evidence shows that AT3 protects against H/R injury in cerebral microvascular endothelial cells via inhibition of HIF-1α,NO and ROS,predominantly through the downregulation of M4-mAChR.The findings offer novel understandings regarding AT3-mediated attenuation of endothelial cell apoptosis and cerebral ischemia/reperfusion injury.

关键词： Hypoxia/reoxygenation Endothelial cell Anisodine hydrobromide Muscarinic acetylcholine receptors Hypoxia-inducible factor-1α

Glutamine synthetase-negative hepatocellular carcinoma has better prognosis and response to sorafenib treatment after hepatectomy

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Chinese Medical Journal 2023年第17期136卷 2066-2076页

作者： Mingyang Shao Qing Tao Yahong Xu Qing Xu Yuke Shu Yuwei Chen junyi shen Yongjie Zhou Zhenru Wu Menglin Chen Jiayin Yang Yujun Shi Tianfu Wen Hong Bu Institute of Clinical Pathology Key Laboratory of Transplant Engineering and ImmunologyNHCWest China HospitalSichuan UniversityChengduSichuan 610041China Laboratory of Liver Transplantation Frontiers Science Center for Disease-Related Molecular NetworkWest China HospitalSichuan UniversityChengduSichuan 610041China Department of Liver Surgery West China HospitalSichuan UniversityChengduSichuan 610041China Department of Pathology West China HospitalSichuan UniversityChengduSichuan 610041China

Background:Glutamine synthetase(GS)and arginase 1(Arg1)are widely used pathological markers that discriminate hepatocellular carcinoma(HCC)from intrahepatic cholangiocarcinoma;however,their clinical significance in HCC remains unclear.Methods:We retrospectively analyzed 431 HCC patients:251 received hepatectomy alone,and the other 180 received sorafenib as adjuvant treatment after hepatectomy.Expression of GS and Arg1 in tumor specimens was evaluated using immunostaining.mRNA sequencing and immunostaining to detect progenitor markers(cytokeratin 19[CK19]and epithelial cell adhesion molecule[EpCAM])and mutant TP53 were also conducted.Results:Up to 72.4%(312/431)of HCC tumors were GS positive(GS+).Of the patients receiving hepatectomy alone,GS negative(GS-)patients had significantly better overall survival(OS)and recurrence-free survival(RFS)than GS+patients;negative expression of Arg1,which is exclusively expressed in GS-hepatocytes in the healthy liver,had a negative effect on prognosis.Of the patients with a high risk of recurrence who received additional sorafenib treatment,GS-patients tended to have better RFS than GS+patients,regardless of the expression status of Arg1.GS+HCC tumors exhibit many features of the established proliferation molecular stratification subtype,including poor differentiation,high alpha-fetoprotein levels,increased progenitor tumor cells,TP53 mutation,and upregulation of multiple tumor-related signaling pathways.Conclusions:GS-HCC patients have a better prognosis and are more likely to benefit from sorafenib treatment after hepatectomy.Immunostaining of GS may provide a simple and applicable approach for HCC molecular stratification to predict prognosis and guide targeted therapy.

关键词： Hepatocellular carcinoma Glutamine synthetase Molecular stratification Targeted therapy

Examining the Factors Affecting Personal Income： An Empirical Study Based on Survey Data in Chinese Cities

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Frontiers of Economics in China-Selected Publications from Chinese Universities 2017年第4期12卷 515-544页

作者： Lihui Wang junyi shen Graduate School of Economics Kobe University Nada Kobe 657-8501 Japan Research Institute for Economics and Business Administration Kobe University Nada Kobe 657-8501 Japan

This paper empirically analyzes the factors affecting personal income in urban China using survey data of the ＂Preference and Life Satisfaction Survey＂ conducted by the Global COE project of Osaka University from 2009 to 2013. We consider education level as an endogenous variable, and both ordinary least squares （OLS） regression and instrumental variable （IV） regression are performed. We find a number of factors, such as sex, age, education, and marriage that significantly affect personal income. In addition, differences between different occupations are also investigated.

关键词： personal income earning function urban China discrimination

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基于事件本体的地震知识库构建研究

图书情报工作 2024年第12期68卷 18-30页

作者：刘佳边俊伊沈胜男吉林大学商学与管理学院长春130012

[目的/意义]构建地震事件本体模型与地震事件知识库,揭示地震事件动态演变过程,为地震事件知识组织提供新的思路,为地震应急管理提供支持与辅助。[方法/过程]利用自然语言处理方法对采集的地震事件数据进行处理;依据本体建模方法,基于... 详细信息

[目的/意义]构建地震事件本体模型与地震事件知识库,揭示地震事件动态演变过程,为地震事件知识组织提供新的思路,为地震应急管理提供支持与辅助。[方法/过程]利用自然语言处理方法对采集的地震事件数据进行处理;依据本体建模方法,基于事件本体模型与灾害事件链式演变关系构建地震事件本体模型;运用Protégé和Neo4j开发地震事件本体和知识库。[结果/结论]实验结果表明,所构建的本体模型能够用于对地震事件的表示与描述,实现地震知识库的知识推理、知识检索与可视化分析功能,为地震事件知识获取与地震应急管理提供支持。

关键词：事件本体地震知识库知识服务灾害链

矩形水凝胶微凹槽的空间约束调控人脐静脉内皮细胞的形态和排列

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四川大学学报（医学版） 2024年第1期55卷 87-94页

作者：江文丽钟健欧阳智沈钧怡邱艳曾烨四川大学华西基础医学与法医学院生物医学工程研究室成都610041

目的本研究拟构建微米级矩形水凝胶凹槽,探究人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)在三维空间约束条件下的形态和排列规律。方法使用四臂-聚乙二醇-丙烯酸酯水凝胶制备宽度为60μm、100μm、140μm的矩形... 详细信息

目的本研究拟构建微米级矩形水凝胶凹槽,探究人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)在三维空间约束条件下的形态和排列规律。方法使用四臂-聚乙二醇-丙烯酸酯水凝胶制备宽度为60μm、100μm、140μm的矩形微凹槽,检测其尺寸和纤连蛋白(fibronectin,FN)黏附情况;在FN包被的微凹槽中接种HUVECs,培养48 h后检测细胞的形态和取向,利用鬼笔环肽标记细胞骨架和激光共聚焦显微镜观察水凝胶微凹槽中HUVECs细胞骨架取向,以无微图案平面为对照。结果构建的水凝胶微凹槽形态均一、结构完整,边缘清晰,宽度误差<3.5%,不同宽度水凝胶微凹槽的深度差异较小,FN黏附均匀,为细胞提供了微图案化的生长界面。对照组中细胞排列杂乱,取向随机,细胞取向角为(46.9±1.8)°,而水凝胶微凹槽中细胞取向角显著减小(P<0.001),但随着水凝胶微凹槽宽度增加而增大,60μm、100μm、140μm水凝胶微凹槽中细胞取向角分别为(16.4±2.8)°、(24.5±3.2)°、(30.3±3.5)°;与对照组相比较(35.7%),不同宽度水凝胶微凹槽中取向角<30°的细胞数量增多(P<0.001),但随着水凝胶微凹槽宽度增加,取向角<30°的细胞数量逐渐减少(79.9%、62.3%、54.7%),而取向角60°~90°的细胞数量逐渐增加(P<0.001)。微凹槽中细胞胞体变小、变圆,细胞沿微凹槽方向排列,细胞骨架排列发生相应改变,对照组中细胞骨架纤维丝排列方向随机,取向角为(45.5±3.7)°,各取向角内骨架纤维数量分布均匀,但60μm、100μm、140μm的水凝胶微凹槽中的细胞骨架蛋白纤维取向角显著降低,分别为(14.4±3.1)°、(24.7±3.5)°、(31.9±3.3)°,不同宽度水凝胶微凹槽中取向角<30°的骨架纤维数量明显增加(P<0.001),但随着水凝胶微凹槽宽度增加,取向角<30°的骨架纤维数量逐渐减少,而取向角60°~90°的骨架纤维数量逐渐增加(P<0.001)。结论水凝胶微凹槽可调控HUVECs形态与取向,在一定程度上可模拟血管内皮细胞的在体微环境,为研究血管内皮细胞的独特生理功能提供了更符合生理条件的实验模型,但三维空间约束影响血管内皮细胞形态和组装的分子机制有待进一步研究。

关键词：微图案血管内皮细胞细胞形态细胞取向细胞骨架

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长江中游宜昌江段铜鱼的种群特征和资源量

水产学报 2024年第4期48卷 130-141页

作者：盖帅帅李君轶沈丽方冬冬危起伟中国水产科学研究院长江水产研究所农业农村部淡水生物多样性保护重点实验室西南大学生命科学学院南京农业大学无锡渔业学院武汉长江中华鲟保护中心

为了解三峡水库蓄水后，长江中游宜昌江段铜鱼的种群动态变化特征，基于2003—2005年、2009—2011年和2017—2019年用流刺网在宜昌江段收集的9 120尾铜鱼样本的基础生物学数据，运用FISATⅡ软件对种群参数和资源量进行估算。结果显示，2... 详细信息

为了解三峡水库蓄水后，长江中游宜昌江段铜鱼的种群动态变化特征，基于2003—2005年、2009—2011年和2017—2019年用流刺网在宜昌江段收集的9 120尾铜鱼样本的基础生物学数据，运用FISATⅡ软件对种群参数和资源量进行估算。结果显示，2003—2005年、2009—2011年和2017—2019年宜昌江段铜鱼体长分布范围分别为118～416、111～409和107～380mm，平均体长（平均值±标准差）为（230.7±49.8）、（217.5±54.3）和（267.2±34.1） mm；体重分布范围分别为10～1 000、19～889和16～816 g，平均体重（平均值±标准差）分别为（195.8±123.6）、（153.2±118.6）和（250.3±84.2） g；生长指数b值分别为3.015 1、3.031 9和2.977 5，均属匀速生长型。由Powell-Wetherall plot法和ELEFANⅠ法拟合渐进体长L∞分别为535、530、500 mm；生长系数k值分别为0.18、0.14和0.15，属中等速率生长型；由体长结构实际种群分析估算铜鱼年资源量分别为1.14×10～6、1.89×10～6和2.10×10～6尾。研究表明，三峡水库蓄水后，宜昌江段铜鱼为匀速生长型，资源数量增加明显，但生长速率减缓，存在小型化趋势。研究结果将为长江禁渔后的渔业资源保护与利用提供科学依据。

关键词：铜鱼种群参数资源量体长股分析宜昌江段

A novel nine gene signature integrates stemness characteristics associated with prognosis in hepatocellular carcinoma

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BIOCELL 2021年第6期45卷 1425-1448页

作者： XINGHONG YAO CHENGQUAN LU junyi shen WENLI JIANG YAN QIU YE ZENG LIANG LI Institute of Biomedical Engineering West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengdu610041China Department of Radiation Oncology Sichuan Cancer Hospital&InstituteSchool of Medicine of University of Electronic Science and Technology of ChinaChengdu610041China Jinzhou City Center for Disease Control and Prevention Jinzhou121000China

Cancer stem cells(CSCs)are heterogeneous with self-renewal and differentiation ability.The mRNA expression-based stemness index(mRNAsi)described the similarity between tumor cells and CSCs,which is positively associated with the poor prognosis of cancer patients.However,the key prognostic genes related to mRNAsi in hepatocellular carcinoma(HCC)remains unclear.A 9-gene signature related to mRNAsi and HCC prognosis including PSMG3,SNRPD1,DTYMK,PIGU,NME1,TXNL4A,IPO4,PES1,and REXO4 was obtained.High expression of this signature indicates poor prognosis of HCC.PIGU was an independent prognostic factor of HCC,which was significantly associated with progression of HCC.Among them,DTYMK and NME1 enriched in pyrimidine metabolism,SNRPD1 and TXNL4A enriched in spliceosome and PIGU enriched in glycosyl phosphatidylinositol(GPI)-anchor biosynthesis pathways.High levels of IPO4,NME1,PES1,PIGU and SNRPD1 were closely associated with metastasis of HCC,and low levels of IPO4,PIGU and REOX4 were significantly associated with sorafenib resistance of HCC.High expression of the 9-gene signature was negatively correlated with the stromal cell infiltration,and positively correlated with specific immune subtypes-related to angiogenesis,M1/M2 macrophage polarization,and M2 response.The 9-gene signature was negatively correlated with the stroma,and SNRPD1 and TXNL4 were positively correlated with immune infiltrate.NME1 was negatively correlated with tumor purity.Therefore,a 9-gene signature related to mRNAsi and poor prognosis in HCC were identified,which can be used as biomarkers for the diagnosis of HCC and functional mechanism exploration of CSCs in HCC.These genes such as IPO4 and PIGU might drive the transition of tumor cells into CSCs which possibly controls the balance between metastasis and drug resistance in HCC.The challenge on balance between metastasis and drug resistance for tumor therapy was firstly reported by the present study.

关键词： Stemness index Hepatocellular carcinoma Prognosis Microenvironment