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Calcium Signaling during Reproduction and Biotrophic Fungal Interactions in Plants

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Molecular Plant 2015年第4期8卷 595-611页

作者： junyi chen Caroline Gutjahr Andrea Bleckmann Thomas Dresselhaus Cell Biology and Plant Biochemistry Biochemie-Zentrum Regensburg University of Regensburg Universitatsstrabe 31 D-93053 Regensburg Germany Faculty of Bio o cly Genet cs Biocenter Martinsried University of Munich (LMU) Grosshaderner Strasse 2-4 D-82152 Martinsried Germany

Many recent studies have indicated that cellular communications during plant reproduction, fungal invasion, and defense involve identical or similar molecular players and mechanisms. Indeed, pollen tube invasion and sperm release shares many common features with infection of plant tissue by fungi and oomycetes, as a tip-growing intruder needs to communicate with the receptive cells to gain access into a cell and tissue. Depending on the comPatibility between cells, interactions may result in defense, inva- sion, growth support, or cell death. Plant cells stimulated by both pollen tubes and fungal hyphae secrete, for example, small cysteine-rich proteins and receptor-like kinases are activated leading to intracellular signaling events such as the production of reactive oxygen species （ROS） and the generation of calcium （Ca^2＋） transients. The ubiquitous and versatile second messenger Ca^2＋ thereafter plays a central and crucial role in modulating numerous downstream signaling processes. In stimulated cells, it elicits both fast and slow cellular responses depending on the shape, frequency, amplitude, and duration of the Ca^2＋ transients. The various Ca^2＋ signatures are transduced into cellular information via a battery of Ca^2＋-binding proteins. In this review, we focus on Ca^2＋ signaling and discuss its occurrence during plant reproduction and interactions of plant cells with biotrophic filamentous microbes. The participation of Ca^2＋ in ROS signaling pathways is also discussed.

关键词： pollen tube fertilization calcium fungal hyphae mycorrhiza compatibility

The Mystery of Mendel＇s Stay-Green： Magnesium Stays Chelated in Chlorophylls

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Molecular Plant 2016年第12期9卷 1556-1558页

作者： chen, junyi Ren, Guodong Kuai, Benke Fudan Univ State Key Lab Genet Engn Sch Life Sci Songhu Rd 2005 Shanghai 200433 Peoples R China Fudan Univ Fudan Ctr Genet Divers & Designing Agr Sch Life Sci Songhu Rd 2005 Shanghai 200433 Peoples R China

Large-scale chlorophyll （Chl） degradation, along with concomi- tant anthocyanin biosynthesis, creates the magnificent canopy scenery in the autumn. Dynamic Chl degradation greatly facili- tates plant growth and development by remobilizing nutrients from senescing organs to rapidly developing ones, seeds and storage organs in particular. In the last decade or so, the major Chl degradation pathway, termed the pheophorbide a oxygenase （PAO） pathway, has been extensively characterized by identifying Chl catabofic genes （CCGs） or enzymes （CCEs）. Chl a is the degradable form of Chls; while Chl b needs to be converted to Chl a before being channeled for degradation （Figure 1A）.

关键词： Revealing Native Transposable

Boosted sustained-release with iron-based MOF-derived mesoporouscarbon-spheres as a nitroimidazole drugs carrier

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Particuology 2024年第3期86卷 269-280页

作者： Danping Wu Jiaying Zou junyi chen Yan Li Na Ma Wei Dai College of Chemistry and Materials Science Zhejiang Normal UniversityJinhua321004China College of Life Sciences Zhejiang Normal UniversityJinhua321004China College of Geography and Environmental Sciences Zhejiang Normal UniversityJinhua321004China

Metal-organic framework(MoF)with a buildable internal structure has aroused great interest focus as self-sacrificing precursors of porous carbon(PC).However,as a drug carrier,the MOF-derived PC developed thus far are generally composed of irregular powder shape due to their crystalline nature,which consequently causing the cerebral infarction,cerebral thrombosis,and other blood *** this article,we propose a novel approach to constructing amorphous carbon microspheres(AcMs)by dis-torting the topological network through hydrothermal treatment precursors of MIL-101(Fe).Then,a distinctive MIL-101(Fe)-derived spherical porous carbons(MSPC)is achieved through high temperature calcination toward *** of the glucose initial concentration and hydrothermal treatment time on the sphericity of the as-prepared mesoporous MsPC were investigated in *** the loading capacities and sustained-release performances of nitroimidazole drugs over MsPC through simulation internal environment of human body at different pH values was systematically *** nitro-imidazole drugs loading rate and release time of MsPC are 10% and 17 h under preferred ***,the MSPC exhibited very low toxicity on Hela cells and 293T cells at the concentrations tested(10-800μg mL^(-1)).This study,therefore,supports the potential of the mesoporous carbon spheres as a carrierfor nitroimidazole drug delivery.

关键词： Ornidazole Metronidazole Carbon spheres Sustained-release performance Biocompatibility

Multimodal critical-scenarios search method for test of autonomous vehicles

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Journal of Intelligent and Connected Vehicles 2022年第3期5卷 167-176页

作者： Tianyue Feng Lihao Liu Xingyu Xing junyi chen Tongji University ShanghaiChina

Purpose–The purpose of this paper is to search for the critical-scenarios of autonomous vehicles(AVs)quickly and comprehensively,which is essential for verification and validation(V&V).Design/methodology/approach–The author adopted the index F1 to quantitative critical-scenarios’coverage of the search space and proposed the improved particle swarm optimization(IPSO)to enhance exploration ability for higher *** with the particle swarm optimization(PSO),there were three *** the initial phase,the Latin hypercube sampling method was introduced for a uniform distribution of *** the iteration phase,the neighborhood operator was adapted to explore more modals with the particles divided into *** the convergence phase,the convergence judgment and restart strategy were used to explore the search space by avoiding local *** with the Monte Carlo method(MC)and PSO,experiments on the artificial function and critical-scenarios search were carried out to verify the efficiency and the application effect of the ***–Results show that IPSO can search for multimodal critical-scenarios comprehensively,with a stricter threshold and fewer samples in the experiment on critical-scenario search,the coverage of IPSO is 14%higher than PSO and 40%higher than ***/value–The critical-scenarios’coverage of the search space is firstly quantified by the index F1,and the proposed method has higher search efficiency and coverage for the critical-scenarios search of AVs,which shows application potential for V&V.

关键词： Autonomous driving system Virtual test Scenario Optimization algorithm

Inhibition of post-trabeculectomy fibrosis via topically instilled antisense oligonucleotide complexes co-loaded with fluorouracil

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Acta Pharmaceutica Sinica B 2020年第9期10卷 1754-1768页

作者： Kuan Jiang junyi chen Lingyu Tai Chang Liu Xishan chen Gang Wei Weiyue Lu Weisan Pan Key Laboratory of Smart Drug Delivery Ministry of EducationDepartment of PharmaceuticsSchool of PharmacyFudan UniversityShanghai 201203China Department of Ophthalmology Eye&ENT HospitalFudan UniversityShanghai 200031China School of Pharmacy Shenyang Pharmaceutical UniversityShenyang 110016China The Institutes of Integrative Medicine of Fudan University Shanghai 200040China Shanghai Engineering Research Center of Immunotherapeutics Shanghai 201203China

Trabeculectomy is the mainstay of surgical glaucoma treatment,while the success rate was unsatistying due to postoperative scarring of the filtering *** countermeasures for scar prevention are intraoperative intervention or repeated subconjunctival ***,we designed a codelivery system capable of transporting fluorouracil and anti-TGF-β2 oligonucleotide to synergistically inhibit fibroblast proliferation via topical *** co-delivery system was built based on a cationic dendrimer core(PAMAM),which encapsulated fluorouracil within hydrophobic cavity and condensed oligonucleotide with surface amino groups,and was further modified with hyaluronic acid and cell-penetrating peptide *** co-delivery system was self-assembled into nanoscale complexes with increased cellular uptake and enabled efficient inhibition on proliferation of fibroblast *** vivo studies on rabbit trabeculectomy models further confirmed the anti-fibrosis efficiency of the complexes,which prolonged survival time of filtering blebs and maintained their height and extent during wound healing process,exhibiting an equivalent effect on scar prevention compared to intraoperative infiltration with *** observation by immunohistochemistry staining and quantitative analysis by Western blotting both suggested that TGF-β2 expression was inhibited by the co-delivery *** study provided a potential approach promising to guarantee success rate of trabeculectomy and prolong survival time of filtering blebs.

关键词： Trabeculectomy Fibrosis prevention Gene delivery Penetratin Fluorouracil

Targeting the LSD1-G9a-ER Stress Pathway as a Novel Therapeutic Strategy for Esophageal Squamous Cell Carcinoma

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Research 2022年第3期2022卷 367-384页

作者： Hongxiao Wang Zijun Song Enjun Xie junyi chen Biyao Tang Fudi Wang Junxia Min The First Affiliated Hospital The Fourth Affiliated HospitalInstitute of Translational MedicineSchool of Public HealthCancer CenterState Key Laboratory of Experimental HematologyZhejiang University School of MedicineHangzhou 310058China The First Affiliated Hospital The Second Affiliated HospitalBasic Medical SciencesSchool of Public HealthSchool of Pharmaceutical ScienceHengyang Medical SchoolUniversity of South ChinaHengyang 421001China Department of Pathology Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityPeople’s Hospital of Henan UniversityZhengzhou 450003China

Despite recent advances in the management and treatment of esophageal squamous cell carcinoma(ESCC),the prognosis remains extremely poor,and current nonsurgical treatment options are *** identify new therapeutic targets,we screened a curated library of epigenetic compounds using a panel of cancer cell lines and found that coinhibiting the histone demethylase LSD1 and the histone methyltransferase G9a potently suppresses cell growth;similar results were obtained by knocking down both LSD1 and G9a ***,we also found that inhibiting LSD1 and G9a significantly decreased tumor growth in a xenograft mouse model with ESCC cell *** examine the clinical relevance of these findings,we performed immunohistochemical analyses of microarray profiling data obtained from human esophageal squamous cancer tissues and found that both LSD1 and G9a are upregulated in cancer tissues compared to healthy tissues,and this increased expression was significantly correlated with poor ***,we discovered that inhibiting LSD1 and G9a induces cell death via S-phase arrest and apoptosis,and cotargeting ER stress pathways increased this effect both in vitro and in *** together,these findings provide compelling evidence that targeting LSD1,G9a,and ER stress-related pathways may serve as a viable therapeutic strategy for ESCC.

关键词： inhibiting Carcinoma viable

On the origin of the low immunogenicity and biosafety of a neutralα-helical polypeptide as an alternative to polyethylene glycol

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Bioactive Materials 2024年第2期32卷 333-343页

作者： Jialing Sun junyi chen Yiming Sun Yingqin Hou Zhibo Liu Hua Lu Beijing National Laboratory for Molecular Sciences Center for Soft Matter Science and EngineeringKey Laboratory of Polymer Chemistry and Physics of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing100871China Beijing National Laboratory for Molecular Sciences Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental ScienceNMPA Key Laboratory for Research and Evaluation of RadiopharmaceuticalsKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing100871China Peking University–Tsinghua University Center for Life Sciences Peking UniversityBeijing100871China

Poly(ethylene glycol)(PEG)is a prominent synthetic polymer widely used in *** its notable success,recent clinical evidence highlights concerns regarding the immunogenicity and adverse effects associated with PEG in PEGylated proteins and lipid *** studies have found a neutral helical polypeptide poly(γ-(2-(2-(2-methoxyethoxy)ethoxy)ethyl L-glutamate),namely L-P(EG3Glu),as a potential alternative to PEG,displaying lower *** comprehensively assess the immunogenicity,distribution,degradation,and biosafety of L-P(EG3Glu),herein,we employ assays including enzyme-linked immunosorbent assay,positron emission tomography-computed tomography,and fluorescent resonance energy *** investigations involve in vivo immune responses,biodistribution,and macrophage activation of interferon(IFN)conjugates tethered with helical L-P(EG3Glu)(L20k-IFN),random-coiled DL-P(EG3Glu)(DL20k-IFN),and PEG(PEG20k-IFN).Key findings encompass:minimal anti-IFN and anti-polymer antibodies elicited by L20k-IFN;length-dependent affinity of PEG to anti-PEG antibodies;accelerated clearance of DL20k-IFN and PEG20k-IFN linked to anti-IFN and anti-polymer IgG;complement activation for DL20k-IFN and PEG20k-IFN but not L20k-IFN;differential clearance with L20k-IFN kidney-based,and DL20k-IFN/PEG20k-IFN accumulation mainly in liver/spleen;enhanced macrophage activation by DL20k-IFN and PEG20k-IFN;L-P(EG3Glu)resistance to proteolysis;and safer repeated administrations of L-P(EG3Glu)in ***,this study offers comprehensive insights into the lower immunogenicity of L-P(EG3Glu)compared to DL-P(EG3Glu)and PEG,supporting its potential clinical use in protein conjugation and nanomedicines.

关键词： helical neutral activation

Isolation of ^(212)Pb from natural thorium for targeted alpha-therapy

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Chinese Chemical Letters 2022年第7期33卷 3474-3477页

作者： junyi chen Mengxin Xu Yu Liu Dongban Duan Yuxiang Han Zhibo Liu Beijing National Laboratory for Molecular Sciences Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental ScienceCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Peking UniversityBeijing 100871China

Targeted alpha-therapy(TAT) is increasingly attractive due to its extraordinary antitumor ***,the supply of α-emitters for TAT is insufficient and under control by a limited number of countries.^(212)Pb is a promising α-emitter with an optimal half-life(10.6 h) and favored decay *** interest,^(212)Pb can be extracted directly from natural thorium,which may be abundant in the mining waste of rare-earth,uranium,***,radioactive thorium waste has been a longstanding environmental challenge that needs immediate *** an on-demand and facile process to isolate ^(212)Pb from natural thorium would be ideal to meet the above challenges,yet is *** theory,the ratio of ^(212)Pb tonatTh is below 10^(-13)in commercially available thorium *** a pilot study,2.2 MBq of ^(212)Pb was successfully extracted from a 5 L solution of thorium nitrate by using a Pb-selective *** radiochemical purity of ^(212)Pb is over 99.9% according to gamma-ray *** purified^(212)Pb was applied to radiolabel a couple of peptides used in clinics(***,TATE and FAPI-04),and the radiochemical yields are>85%.Of note,^(212)Pb can be repeatedly separated from the thorium solution every 2 *** summary,a practical and scalable method was developed to isolate^(212)Pb for potentially clinical use,which may be of great importance as it does not require either cyclotron or nuclear reactor.

关键词： ^(212)Pb Targeted alpha-therapy Radiolabeling Radionuclide generator Solid-phase extraction

Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging

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Chinese Chemical Letters 2024年第9期35卷 183-186页

作者： Zhixue Liu Haiqi chen Lijuan Guo Xinyao Sun Zhi-Yuan Zhang junyi chen Ming Dong Chunju Li Tianjin Key Laboratory of Structure and Performance for Functional Molecules College of ChemistryTianjin Normal UniversityTianjin 300387China

Multicharged supramolecular assemblies based on luminescent macrocycle play an important role in extending their optical properties and ***,we reported macrocyclic supramolecular assemblies based on luminescent terphen[3]arene sulfate(TP[3]AS)and tetraphenylethylene pyridinium(TPE-4Py)through electrostatic interactions,host-guest encapsulation andπ-πstacking interactions.F?rster resonance energy transfer(FRET)process from TP[3]AS to TPE-4Py was achieved with the energy transfer efficiency of 99.9%,accompanied by TPE-4Py fluorescence emission bathochromic shifted of 15 nm and enhanced by 1.68 times in PBS *** contrast,other non-luminescent sulfato-β-cyclodextrin and sulfobutylether-β-cyclodextrin only can enhance the fluorescence intensity of TPE-4Py without bathochromic *** to the strong fluorescence and good stability of TPE-4Py@TP[3]AS,it can be used for optical imaging in living cells,which provided an effective approach for the construction of assembling-confined luminescent biomaterials.

关键词： Luminescent macrocycle Terphenarene sulfate Multicharged assembly Multivalent interactions Forster resonance energy transfer Cell imaging